Kinetics of biodegradation of phenol and a polyphenolic compound by a mixed culture containing Pseudomonas aeruginosa and Bacillus subtilis

ZusammenfassungZentrales Bauteil des Genius® 90 Therapie Systems ist der sogenannte Genius-Tank, dem die frische Dialyseflüssigkeit entnommen und in den die verbrauchte Lösung nach der Dialyse zurückgeführt wird. Daher kommt der sicheren Aufbereitung des Systems eine besondere Bedeutung zu. Hierfür wird ein Aufbereitungsverfahren unter Verwendung von UV-Licht in Kombination mit einem chemischen Desinfektionsmittel angewendet. Ziel der hier beschriebenen Untersuchung war es, die Wirkungsbreite und Wirkungstiefe dieses Aufbereitungsverfahrens unter praxisnahen Phase-3-Bedingungen zu ermitteln. Dazu wurde das Gerät mit Mikroorganismen und Viren künstlich kontaminiert und die Wirkung der einzelnen Verfahrensschritte ermittelt. Im Gegensatz zu der üblichen Vorgehensweise praxisnaher Untersuchungen machen Aufbereitungsverfahren medizinischer Geräte unter Phase-3-Kriterien meist eine neuartige Arbeitsweise erforderlich – im Falle der hier vorgestellten Untersuchung sogar die Konstruktion eines speziellen Geräts zur Platzierung von Keimträgen im Genius-Tank. Im Ergebnis konnte gezeigt werden, dass bereits UV-Licht allein sowie in Kombination mit einem chemischen Desinfektionsmittel unter praxisnahen Bedingungen eine sichere Wirksamkeit gegen Bakterien (Pseudomonas aeruginosa) und bakterielle Sporen (Bacillus subtilis), Schimmelpilze (Aspergillus brasiliensis) und Viren (Murines Parvovirus) besitzt.

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In Vitro Evaluation of Antimicrobial Efficacy of Iontophoresis against Entercoccus faecalis, Candida albicans, Pseudomonas aeruginosa and Bacillus subtilis

Journal of Oral Biosciences ◽

10.2330/joralbiosci.51.91 ◽

2009 ◽

Vol 51 (2) ◽

pp. 91-96

Author(s):

Takao Oyama ◽

Masako H. Nakano ◽

Takashi Arai ◽

Daisuke Kato ◽

Nobuko Maeda

Keyword(s):

Pseudomonas Aeruginosa ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Antimicrobial Efficacy ◽

In Vitro Evaluation

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The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.9.2.12 ◽

2019 ◽

Vol 9 (02) ◽

Author(s):

Hussein A Kadhum ◽

Thualfakar H Hasan2

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Bacterial Growth ◽

Broad Spectrum ◽

Inhibitory Activity ◽

Bacterial Pathogens ◽

Inhibitory Ability ◽

Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

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Production of Carrot Pomace Fortified with Mucilage, Fibrinolytic Enzyme and Probiotics by Solid-state Fermentation Using the Mixed Culture of Bacillus subtilis and Leuconostoc mesenteroides

Preventive Nutrition and Food Science ◽

10.3746/jfn.2009.14.4.335 ◽

2009 ◽

Vol 14 (4) ◽

pp. 335-342 ◽

Cited By ~ 4

Author(s):

Hye-Won Jung ◽

Sam-Pin Lee

Keyword(s):

Bacillus Subtilis ◽

Solid State ◽

Mixed Culture ◽

Solid State Fermentation ◽

Leuconostoc Mesenteroides ◽

Fibrinolytic Enzyme

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Kinetic Analysis of tRNA-Directed Transcription Antitermination of the Bacillus subtilis glyQS Gene In Vitro

Journal of Bacteriology ◽

10.1128/jb.186.16.5392-5399.2004 ◽

2004 ◽

Vol 186 (16) ◽

pp. 5392-5399 ◽

Cited By ~ 38

Author(s):

Frank J. Grundy ◽

Tina M. Henkin

Keyword(s):

Bacillus Subtilis ◽

Assay System ◽

Nascent Transcript ◽

Vitro Assay ◽

T Box ◽

Transcription Antitermination ◽

Transcriptional Pausing ◽

Transcription Complexes ◽

Kinetics Of

ABSTRACT Binding of uncharged tRNA to the nascent transcript promotes readthrough of a leader region transcription termination signal in genes regulated by the T box transcription antitermination mechanism. Each gene in the T box family responds independently to its cognate tRNA, with specificity determined by base pairing of the tRNA to the leader at the anticodon and acceptor ends of the tRNA. tRNA binding stabilizes an antiterminator element in the transcript that sequesters sequences that participate in formation of the terminator helix. tRNAGly-dependent antitermination of the Bacillus subtilis glyQS leader was previously demonstrated in a purified in vitro assay system. This assay system was used to investigate the kinetics of transcription through the glyQS leader and the effect of tRNA and transcription elongation factors NusA and NusG on transcriptional pausing and antitermination. Several pause sites, including a major site in the loop of stem III of the leader, were identified, and the effect of modulation of pausing on antitermination efficiency was analyzed. We found that addition of tRNAGly can promote antitermination as long as the tRNA is added before the majority of the transcription complexes reach the termination site, and variations in pausing affect the requirements for timing of tRNA addition.

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Bactericidal Activity, Absence of Serum Effect, and Time-Kill Kinetics of Ceftazidime-Avibactam against β-Lactamase-Producing Enterobacteriaceae and Pseudomonas aeruginosa

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02894-14 ◽

2014 ◽

Vol 58 (9) ◽

pp. 5297-5305 ◽

Cited By ~ 55

Author(s):

Tiffany R. Keepers ◽

Marcela Gomez ◽

Chris Celeri ◽

Wright W. Nichols ◽

Kevin M. Krause

Keyword(s):

Pseudomonas Aeruginosa ◽

Human Serum ◽

Bactericidal Activity ◽

Minimum Bactericidal Concentration ◽

Wild Type ◽

Content Type ◽

Positive Isolate ◽

New Treatment ◽

Time Kill ◽

Kinetics Of

ABSTRACTAvibactam, a non-β-lactam β-lactamase inhibitor with activity against extended-spectrum β-lactamases (ESBLs), KPC, AmpC, and some OXA enzymes, extends the antibacterial activity of ceftazidime against most ceftazidime-resistant organisms producing these enzymes. In this study, the bactericidal activity of ceftazidime-avibactam against 18Pseudomonas aeruginosaisolates and 15Enterobacteriaceaeisolates, including wild-type isolates and ESBL, KPC, and/or AmpC producers, was evaluated. Ceftazidime-avibactam MICs (0.016 to 32 μg/ml) were lower than those for ceftazidime alone (0.06 to ≥256 μg/ml) against all isolates except for 2P. aeruginosaisolates (1blaVIM-positive isolate and 1blaOXA-23-positive isolate). The minimum bactericidal concentration/MIC ratios of ceftazidime-avibactam were ≤4 for all isolates, indicating bactericidal activity. Human serum and human serum albumin had a minimal effect on ceftazidime-avibactam MICs. Ceftazidime-avibactam time-kill kinetics were evaluated at low MIC multiples and showed time-dependent reductions in the number of CFU/ml from 0 to 6 h for all strains tested. A ≥3-log10decrease in the number of CFU/ml was observed at 6 h for allEnterobacteriaceae, and a 2-log10reduction in the number of CFU/ml was observed at 6 h for 3 of the 6P. aeruginosaisolates. Regrowth was noted at 24 h for some of the isolates tested in time-kill assays. These data demonstrate the potent bactericidal activity of ceftazidime-avibactam and support the continued clinical development of ceftazidime-avibactam as a new treatment option for infections caused byEnterobacteriaceaeandP. aeruginosa, including isolates resistant to ceftazidime by mechanisms dependent on avibactam-sensitive β-lactamases.

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Growth of Salmonella Enteritidis in the presence of quorum sensing signaling compounds produced by Pseudomonas aeruginosa

International Journal of Food Engineering ◽

10.1515/ijfe-2021-0089 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Weijia He ◽

Huamei Yang ◽

Xiang Wang ◽

Hongmei Li ◽

Qingli Dong

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Salmonella Enteritidis ◽

Culture Supernatant ◽

Lag Time ◽

Bacterial Communication ◽

Homoserine Lactones ◽

Positive Effects ◽

Kinetics Of

Abstract Quorum sensing (QS) can exist in food-related bacteria and potentially affect bacterial growth through acyl-homoserine lactones (AHLs). To verify the role of QS compounds in the cell-free supernatant, this study examined the effect of supernatant extracted from Pseudomonas aeruginosa culture on the growth kinetics of Salmonella Enteritidis. The results showed that the lag time (λ) of S. Enteritidis was apparently reduced (p < 0.05) under the influence of P. aeruginosa culture supernatant compared with the S. Enteritidis culture supernatant. HPLC-MS/MS test demonstrated that AHLs secreted by P. aeruginosa were mainly C14-HSL with a content of 85.71 μg/mL and a small amount of 3-oxo-C12-HSL. In addition, the commercially synthetic C14-HSL had positive effects on the growth of S. Enteritidis, confirming once again that the growth of S. Enteritidis was affected by AHL metabolized by other bacteria and the complexity of bacterial communication.

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