scholarly journals Cytotoxic and Micronuclei Inducing Effects of Petroleum Ether Fraction of Leaf Aqueous Extract of Clerodendrum viscosum Vent. in Allium cepa Root Tip Cells

CYTOLOGIA ◽  
2021 ◽  
Vol 86 (3) ◽  
pp. 261-266
Author(s):  
Sujit Roy ◽  
Lalit Mohan Kundu ◽  
Gobinda Chandra Roy ◽  
Manabendu Barman ◽  
Sanjib Ray
Keyword(s):  
Petroleum Ether ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Root Tip ◽  
Petroleum Ether Fraction ◽  
Leaf Aqueous Extract ◽  
Ether Fraction ◽  
Clerodendrum Viscosum ◽  
Tip Cells ◽  
Root Tip Cells

scholarly journals Cytotoxic and micronuclei inducing effects of petroleum ether fraction of leaf aqueous extract of Clerodendrum viscosum Vent. in Allium cepa root tip cells

2021 ◽  
Author(s):  
Sujit Roy ◽  
Lalit Mohan Kundu ◽  
Gobinda Chandra Roy ◽  
Manabendu Barman ◽  
Sanjib Ray
Keyword(s):  
Petroleum Ether ◽  
Allium Cepa ◽  
Aqueous Extract ◽  
Root Tip ◽  
Petroleum Ether Fraction ◽  
Leaf Aqueous Extract ◽  
Ether Fraction ◽  
Clerodendrum Viscosum ◽  
Tip Cells ◽  
Root Tip Cells

Clerodendrum viscosum is a traditionally used medicinal plant. The present study aimed to analyze a detailed cytotoxic effect of the nonpolar petroleum ether fraction (AQPEF) of leaf aqueous extract of C. viscosum Vent. (LAECV) in Allium cepa root tip cells. The LAECV was fractionated with petroleum ether and tested for A. cepa toxicity at early hours (2 and 4 h treatment) at concentrations 0, 0.050, 0.100, 0.150 and 0.200 mg mL-1. The highest aberrant cell percentage (10.45%) was scored from 0.150 mg mL-1 followed by0.100 mg mL-1 (8.75%) concentration at 4 h treated samples. The AQPEF treatment induced a significant (p<0.0001) increase in micronuclei frequency; 4.31, 5.08, 5.05 and 3.05% respectively at concentrations 0.50, 0.100, 0.150, and 0.200 mg mL-1. The highest polyploid frequency (20.14%) induced with 0.100 mg mL-1 of AQPEF at 16 h recovery. 0.150 mg mL-1 is the most effective concentration of AQPEF to decipher its activity similar to colchicine (0.150 mg mL-1). In summary, the present study indicates petroleum ether is suitable for extraction of the active phytochemicals of LAECV having cytotoxic effects on A. cepa root tip cells. The AQPEF has colchicine like micronuclei, polyploidy, and mitotic abnormality inducing potentials in A. cepa root apical meristem cells.

scholarly journals Pro-metaphase arrest, polyploidy, micronuclei, and mitotic abnormality inducing agents’ isolation from leaf aqueous extract of Clerodendrum viscosum Vent

2020 ◽  
Author(s):  
Sujit Roy ◽  
Lalit Mohan Kundu ◽  
Gobinda Chandra Roy ◽  
Manabendu Barman ◽  
Sanjib Ray
Keyword(s):  
Root Growth ◽  
Petroleum Ether ◽  
Aqueous Extract ◽  
Root Tip ◽  
Root Growth Inhibition ◽  
Leaf Aqueous Extract ◽  
Clerodane Diterpenoids ◽  
Clerodendrum Viscosum ◽  
Tip Cells ◽  
Root Tip Cells

AbstractClerodendrum viscosum is a traditionally used medicinal plant and the earlier reports indicate its leaf aqueous extract (LAECV) contains metaphase arresting, cell cycle delay, and mitotic abnormality inducing active principles. The present study aimed to isolate pro-metaphase arresting, polyploidy, micronuclei, and mitotic abnormality inducing active principles of LAECV. The LAECV was successively fractionated as petroleum ether (PEF), chloroform (CHF), and ethyl acetate (EAF) fractions. All the extract fractions were tested for Allium cepa and Triticum aestivum root swelling and root growth inhibition analyses. The petroleum ether fraction was selected for further cytotoxicity analysis on A. cepa root tip cells and was processed for detection of the active principles through HPLC, LC-MS, GC-MS, and IR analyses. The comparative seedlings’ root growth and swelling patterns indicate the bioactive principles are effectively fractionated in PEF and GC-MS analysis revealed the presence of Clerodin (m/z 434.3), 15-hydroxy-14, 15-dihydroclerodin (m/z 452), 15-methoxy-14, 15-dihydroclerodin (m/z 466), and 14, 15-dihydroclerodin (m/z 436) with a retention time of 14.038, 14.103, 14.480 and 14.655 respectively. Thus the present study explores clerodane diterpenoids of LAECV as pro-metaphase arresting, polyploidy, micronuclei, and mitotic abnormality inducing active principles.

Sunlight decreased genotoxicity of azadirachtin on root tip cells of Allium cepa and Eucrosia bicolor

2010 ◽  
Vol 73 (5) ◽  
pp. 949-954 ◽  
Author(s):  
W. Kwankua ◽  
S. Sengsai ◽  
C. Kuleung ◽  
N. Euawong
Keyword(s):  
Allium Cepa ◽  
Root Tip ◽  
Tip Cells ◽  
Root Tip Cells

Cytotoxicity of Spent Pot Liner on Allium cepa root tip cells: A comparative analysis in meristematic cell type on toxicity bioassays

2016 ◽  
Vol 133 ◽  
pp. 442-447 ◽  
Author(s):  
Marcel José Palmieri ◽  
Larissa Fonseca Andrade-Vieira ◽  
José Marcello Salabert Campos ◽  
Leonardo dos Santos Gedraite ◽  
Lisete Chamma Davide
Keyword(s):  
Comparative Analysis ◽  
Allium Cepa ◽  
Meristematic Cell ◽  
Root Tip ◽  
Cell Type ◽  
Tip Cells ◽  
Root Tip Cells

EFFECTS OF NICKEL SULFATE ON ROOT GROWTH AND NUCLEOLI IN ROOT TIP CELLS OF ALLIUM CEPA

1994 ◽  
Vol 42 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Donghua Liu ◽  
Wusheng Jiang ◽  
Lin Guo ◽  
Yuqing Hao ◽  
Cheng Lu ◽  
...  
Keyword(s):  
Root Growth ◽  
Allium Cepa ◽  
Silver Staining ◽  
Nickel Sulfate ◽  
Inhibitory Effect ◽  
Root Tip ◽  
Staining Reaction ◽  
Nucleolar Material ◽  
Tip Cells ◽  
Root Tip Cells

The effects of different concentrations of nickel sulfate on root growth and nucleoli in root tip cells of Allium cepa were studied. The concentrations of nickel sulfate (NiSO4 · 7H2O) used were in the range of 10−7-10−1M. The results showed that nickel sulfate has a stimulatory effect on root growth at lower concentrations, and an inhibitory effect at higher concentrations. Nickel has toxic effects on nucleoli at higher concentrations. Phenomena we observed were irregularly shaped nucleoli, weaker silver staining reaction at the periphery of the nucleolus, and extrusion of nucleolar material from nuclei into the cytoplasm after treatment with higher concentrations of Ni.

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