scholarly journals Bacteremia caused by Helicobacter cinaedi: a case report

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Kateryna Sidak ◽  
Ramón Pérez-Tanoira ◽  
Peña Gomez-Herruz
Keyword(s):  
Ulcerative Colitis ◽  
Blood Culture ◽  
Direct Analysis ◽  
Mucosal Damage ◽  
Culture Conditions ◽  
Culture Bottle ◽  
Gram Negative ◽  
Helicobacter Cinaedi ◽  
Maldi Tof ◽  
Immunosuppressed Patients

Abstract Objectives Helicobacter cinaedi is a Gram-negative, spiral-shaped bacterium that primarily affects immunosuppressed patients. Case presentation A 49-year-old patient with ulcerative colitis diagnosed in 1992, who presented to the ED of our hospital with fever and testicular complaints. The patient was discharged with a diagnosis of left-sided acute epididymitis, which was probably sexually transmitted. At the ED, he was administered intravenous Ceftriaxone and discharged with a prescription of doxycycline for 10 days, with a good progress. Aerobic cultures were positive at three days from collection. Gram staining showed Gram-negative, corkscrew-shaped bacteria. The analysis of the blood culture bottles, and the colonies grown in Campylosel agar incubated in microaerophilic conditions at 42 °C were identified as H. cinaedi on the Maldi-TOF Biotyper 3.0 system (Bruker Diagnostics Inc.). Conclusions Direct analysis of the blood culture bottle on the Maldi-TOF system allowed for the identification of the etiology of the bacteremia since H. cinaedi could not have been grown in standard culture conditions. The treatment of this infection is a matter of debate; however, the combination of ceftriaxone with doxycycline can be ineffective for bacteremia caused by H. cinaedi infection since it occurs by the translocation of the bacteria from the gastrointestinal tract. This type of bacteremia is associated with intestinal mucosal damage secondary to ulcerative colitis, and it primarily affects immunosuppressed patients.

Acid-fast bacilli smear test of a blood culture sample for the diagnosis of disseminated Mycobacterium genavense infection: A case report

2021 ◽  
Vol 32 (5) ◽  
pp. 483-485
Author(s):  
Yo Murata ◽  
Nobuaki Mori ◽  
Narito Kagawa ◽  
Kentaro Okuma ◽  
Shinji Yoshida ◽  
...  
Keyword(s):  
Blood Culture ◽  
Culture Conditions ◽  
Nontuberculous Mycobacterium ◽  
Sequencing Analysis ◽  
Culture Methods ◽  
Smear Test ◽  
Acid Fast Bacilli ◽  
Mycobacterium Genavense ◽  
Old Japanese ◽  
Immunosuppressed Patients

Mycobacterium genavense, a nontuberculous Mycobacterium, is found in immunosuppressed patients, particularly in those with HIV. Mycobacterium genavense incubation under standard culture conditions is difficult, and its identification is challenging using routine culture methods. Herein, we report the case of a 40-year-old Japanese man with HIV presenting with disseminated M. genavense infection. An analysis using an automated blood culture system did not show positive signals during 6 weeks of incubation. However, an acid-fast bacilli smear of his blood sample was positive for the bacterium. Mycobacterium genavense was identified using sequencing analysis, targeting the heat shock protein 65 gene. The patient recovered from the infection, following antibiotic therapy for 18 months. Under suspicion of disseminated M. genavense infection and the absence of bacterial growth in blood culture samples, an acid-fast bacilli smear test of the sample may be useful for timely diagnosis.

scholarly journals Lysinibacillus massiliensis Isolated from the Synovial Fluid: A Case Report

2020 ◽  
Vol 25 (4) ◽  
pp. 595-598
Author(s):  
Canan Eryıldız ◽  
Kıymet Tabakçıoğlu ◽  
Sezgin Kehaya ◽  
Nermin Şakru ◽  
Şaban Gürcan
Keyword(s):  
Synovial Fluid ◽  
Blood Culture ◽  
Blood Culture Bottle ◽  
Negative Staining ◽  
Phenotypic Characterization ◽  
Local Alignment ◽  
Identification System ◽  
Culture Bottle ◽  
Gram Negative ◽  
Microbiological Methods

Lysinibacillus massiliensis is an aerobic, endospore-forming, gram-negative staining bacterium with peritrichous flagella belonging to the Bacillaceae family. A few cases of L. massiliensis isolated from the cerebrospinal fluid and tissue have been reported. In this study, we aimed to describe a case of L. massiliensis isolated from the synovial fluid. The synovial fluid from a 74-year-old female patient was inoculated into blood culture bottle. Gram-negative rods were observed in a gram-stained smear from a positive blood culture bottle. The bacterium was identified as Lysinibacillus sphaericus/Lysinibacillus fusiformis, with a probability of 89% using an automated bacterial identification system (VITEK2; Biomerieux, France). Subsequently, 16S rRNA gene sequencing was performed, and the sequence was analyzed using the Basic Local Alignment Search Tool. The sequence had 99.9% (1426/1427) identity with the strain L. massiliensis (GenBank ID: NR_043092.1). To our knowledge, this is the first reported case of L. massiliensis isolated from the synovial fluid. When an endospore-forming gram-negative staining bacterium can not be identified by phenotypic characterization, L. massiliensis should be considered, and different microbiological methods should be used for identification.

scholarly journals Evaluation of a new Rapid Antimicrobial Susceptibility system for Gram-negative and Gram-positive bloodstream infections: Speed and accuracy of Alfred 60 AST

2019 ◽  
Author(s):  
Vanesa Anton-Vazquez ◽  
Adjepong Samuel ◽  
Suarez Cristina ◽  
Planche Timothy
Keyword(s):  
Blood Culture ◽  
Patient Care ◽  
Antimicrobial Susceptibility ◽  
Antibiotic Sensitivity ◽  
Positive Culture ◽  
Bloodstream Infections ◽  
Blood Stream ◽  
Gram Positive ◽  
Culture Bottle ◽  
Gram Negative

Abstract Background Blood stream infections (BSIs) are a major cause of morbidity and mortality. The time from taking blood cultures to obtain results of antibiotic sensitivity can be up to five days which impacts patient care. The Alfred 60 AST™ can reduce laboratory time from positive culture bottle to susceptibility results from 16-25 hours to 5-6 hours, transforming patient care. Objective To evaluate the diagnostic accuracy of a rapid antimicrobial susceptibility system, the Alfred 60 AST™, in clinical isolates from patients with BSIs and confirm time to results. Methods 301 Gram-negative and 86 Gram-positive isolates were analysed directly from positive blood culture bottles following Gram staining. Antimicrobial susceptibility results and time-to-results obtained by rapid Alfred 60 AST system and BD Phoenix were compared . Results A total of 2,196 antimicrobial susceptibility test results (AST) were performed: 1,863 Gram-negative and 333 Gram-positive. AST categorical agreement (CA) for Alfred 60 AST™ was 95% (1772/1863) for Gram-negative and 89% (295/333) for Gram-positive isolates. Gram-negative CA: ampicillin 96% (290/301); ciprofloxacin 95% (283/297); ceftriaxone 96% (75/78); meropenem 97% (288/297); piperacillin-tazobactam 95% (280/295); gentamicin 94% (279/297) and amikacin 93% (277/298). The median time to susceptibility results from blood culture flagging positive was 6.3 h vs 20 h (p<0.01) for Alfred system vs BD Phoenix™. Conclusion Alfred 60 AST system greatly reduced time to antimicrobial susceptibility results in Gram-negative and Gram-positive BSIs with good performance and cost, particularly for Gram-negative bacteraemia.

scholarly journals Evaluation of a new Rapid Antimicrobial Susceptibility system for Gram-negative and Gram-positive bloodstream infections: Speed and accuracy of Alfred 60 AST

2019 ◽  
Author(s):  
Vanesa Anton-Vazquez ◽  
Adjepong Samuel ◽  
Suarez Cristina ◽  
Planche Timothy
Keyword(s):  
Blood Culture ◽  
Patient Care ◽  
Antimicrobial Susceptibility ◽  
Antibiotic Sensitivity ◽  
Positive Culture ◽  
Bloodstream Infections ◽  
Rapid Diagnostics ◽  
Gram Positive ◽  
Culture Bottle ◽  
Gram Negative

Abstract Background: Blood stream infections (BSIs) are a major cause of morbidity and mortality. The time from taking blood cultures to obtain results of antibiotic sensitivity can be up to five days which impacts patient care. The Alfred 60 AST™ can reduce laboratory time from positive culture bottle to susceptibility results from 16-25 hours to 5-6 hours, transforming patient care. To evaluate the diagnostic accuracy of a rapid antimicrobial susceptibility system, the Alfred 60 AST™, in clinical isolates from patients with BSIs and confirm time to results. 301 Gram-negative and 86 Gram-positive isolates were analysed directly from positive blood culture bottles following Gram staining. Antimicrobial susceptibility results and time-to-results obtained by rapid Alfred 60 AST system and BD Phoenix were compared . Results: A total of 2,196 antimicrobial susceptibility test results (AST) were performed: 1,863 Gram-negative and 333 Gram-positive. AST categorical agreement (CA) for Alfred 60 AST™ was 95% (1772/1863) for Gram-negative and 89% (295/333) for Gram-positive isolates. Gram-negative CA: ampicillin 96% (290/301); ciprofloxacin 95% (283/297); ceftriaxone 96% (75/78); meropenem 97% (288/297); piperacillin-tazobactam 95% (280/295); gentamicin 94% (279/297) and amikacin 93% (277/298). The median time to susceptibility results from blood culture flagging positive was 6.3 h vs 20 h (p<0.01) for Alfred system vs BD Phoenix™. Conclusion: Alfred 60 AST system greatly reduced time to antimicrobial susceptibility results in Gram-negative and Gram-positive BSIs with good performance and cost, particularly for Gram-negative bacteraemia. Keywords: Rapid diagnostics. Bloodstream infection. Bacteraemia. Antimicrobial susceptibility testing. Gram-negative bacteria. Gram-positive bacteria

scholarly journals Proof of Concept of Culturomics Use of Time of Care

2020 ◽  
Vol 10 ◽  
Author(s):  
Sabrina Naud ◽  
Saber Khelaifia ◽  
Maxime Descartes Mbogning Fonkou ◽  
Niokhor Dione ◽  
Jean-Christophe Lagier ◽  
...  
Keyword(s):  
Blood Culture ◽  
Stool Sample ◽  
Incubation Time ◽  
Bacterial Species ◽  
Blood Culture Bottle ◽  
Culture Bottle ◽  
Prolonged Incubation ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Culturomics, a high throughput culture method with rapid identification of the colonies by Matrix Assisted Laser Desorption Ionization/Time Of Flight Mass Spectrometry (MALDI-TOF MS), has demonstrated its contribution to the exploration of the gut microbiota over the past 10 years. However, the cost, work time and workload, considerably limit its use on a large scale or emergency context. Here, by testing two different stool samples, including a stool sample from a patient requiring rapid immunotherapy treatment, we tested a new fast culturomic protocol using two pre-incubation media, blood culture bottle and YCFA modified medium. Both media were supplemented with 2 ml of rumen fluid filtered at 0.2 μm and 2 ml of defibrinated and sterile sheep blood. Unlike the standard culturomics, subculturing of blood culture bottle were performed at reduced incubation time (3 h, 6 h, 9 h, 24 h) and at a longer incubation time (3 days, 7 days, and 10 days) at 37°C. By testing 5,200 colonies per MALDI-TOF MS and obtaining a comparable number of cultured bacterial species (131 to 143) in a stool sample, this new protocol reduced the number of colonies tested by 57%, working time by 78.6% and cost by 72.2%. In addition, we highlighted that the proportion of strict anaerobic species has increased by 24%, known to be the preferential targets for biotherapy, including Faecalibacterium prausnitzii, Akkermansia muciniphila, Christensenella minuta, and Phascolarctobacterium faecium. Finally, this work showed that some bacterial species grew earlier but disappeared with prolonged incubation times.

Superiority of SDS lysis over saponin lysis for direct bacterial identification from positive blood culture bottle by MALDI-TOF MS

2016 ◽  
Vol 11 (5-6) ◽  
pp. 1600131 ◽  
Author(s):  
Yvan Caspar ◽  
Cécile Garnaud ◽  
Mariya Raykova ◽  
Sébastien Bailly ◽  
Marie Bidart ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Blood Culture Bottle ◽  
Bacterial Identification ◽  
Culture Bottle ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Evaluation of a new Rapid Antimicrobial Susceptibility system for Gram-negative and Gram-positive bloodstream infections: Speed and accuracy of Alfred 60 AST

2019 ◽  
Author(s):  
Vanesa Anton-Vazquez ◽  
Adjepong Samuel ◽  
Suarez Cristina ◽  
Planche Timothy
Keyword(s):  
Blood Culture ◽  
Patient Care ◽  
Antimicrobial Susceptibility ◽  
Antibiotic Sensitivity ◽  
Positive Culture ◽  
Bloodstream Infections ◽  
Rapid Diagnostics ◽  
Gram Positive ◽  
Culture Bottle ◽  
Gram Negative

Abstract Background: Blood stream infections (BSIs) are a major cause of morbidity and mortality. The time from taking blood cultures to obtain results of antibiotic sensitivity can be up to five days which impacts patient care. The Alfred 60 AST™ can reduce laboratory time from positive culture bottle to susceptibility results from 16-25 hours to 5-6 hours, transforming patient care. Objective: To evaluate the diagnostic accuracy of a rapid antimicrobial susceptibility system, the Alfred 60 AST™, in clinical isolates from patients with BSIs and confirm time to results. Methods: 301 Gram-negative and 86 Gram-positive isolates were analysed directly from positive blood culture bottles following Gram staining. Antimicrobial susceptibility results and time-to-results obtained by rapid Alfred 60 AST system and BD Phoenix were compared . Results : A total of 2,196 antimicrobial susceptibility test results (AST) were performed: 1,863 Gram-negative and 333 Gram-positive. AST categorical agreement (CA) for Alfred 60 AST™ was 95% (1772/1863) for Gram-negative and 89% (295/333) for Gram-positive isolates. Gram-negative CA: ampicillin 96% (290/301); ciprofloxacin 95% (283/297); ceftriaxone 96% (75/78); meropenem 97% (288/297); piperacillin-tazobactam 95% (280/295); gentamicin 94% (279/297) and amikacin 93% (277/298). The median time to susceptibility resultsfrom blood culture flagging positive was 6.3 h vs 20 h ( p<0.01 ) for Alfred system vs BD Phoenix™. Conclusion: Alfred 60 AST system greatly reduced time to antimicrobial susceptibility results in Gram-negative and Gram-positive BSIs with good performance and cost, particularly for Gram-negative bacteraemia. Keywords: Rapid diagnostics. Bloodstream infection. Bacteraemia. Antimicrobial susceptibility testing. Gram-negative bacteria. Gram-positive bacteria

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