Response of nitrogen assimilating enzymes during in vitro culture of Argyrolobium roseum

Biologia ◽  
2015 ◽  
Vol 70 (4) ◽  
Author(s):  
Darima Habib ◽  
Muhammad Zia ◽  
Yamin Bibi ◽  
Bilal Haider Abbasi ◽  
Muhammad Fayyaz Chaudhary
Keyword(s):  
Life Cycle ◽  
Seed Germination ◽  
In Vitro Culture ◽  
Growth And Development ◽  
Plant Cells ◽  
Root Explants ◽  
Reverse Pattern ◽  
Developmental Phases ◽  
Root Tissues

AbstractNitrogen assimilating enzymes play curtail role during un-differentiation and re-differentiation of plant cells. To investigate role and pattern of glutamine synthetase (GS), nitrate reductase (NR) and glutamate dehydrogenase (GDH) during in vitro life cycle of Argyrolobium roseum this study was conducted. The concentrations of these enzymes were determined during seed germination; callus induction from leaf, stem and root explants; shoot regeneration from callus; root development and acclimatization stages. GS and NR enzymes showed ascending pattern during in vitro plant development from seed while GDH concentration decreased during this process. Completely reverse pattern was showed by these enzymes during callogenesis and proliferation phase. Increase in GS and NR activities was noticed in regenerated leaves and stem during shoots and roots developmental phases; and vice verse for GDH. The acclimatization stress also up lifted NR and GS activities in leaf, stem and root tissues. This study highlights the importance of nitrogen assimilating enzymes (NR, GS, and GDH) during growth and development of A. roseum in vitro culture.

In vitro propagation of the bay laurel (Laurus nobilis.L) in Morocco

2014 ◽  
Vol 4 (3) ◽  
pp. 96-103
Author(s):  
Abdelali Chourfi ◽  
Tajelmolk Alaoui ◽  
Ghizlane Echchgadda
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Aerial Part ◽  
Basal Medium ◽  
Axillary Buds ◽  
Laurus Nobilis

Laurus nobilis L. is among the species which are most threatened by massive degradation in Morocco. The multiplication by seed or by cuttings gives very low percentages of recovery that is insufficient to meet the demand of growing market. In vitro culture proves to be a tremendous asset to solve this problem. Our work has focused on the study of seed germination of this species and its multiplication from microcuttings. Finally, we studied the ac-climatization ability of the plantlets resulting from this germination. The study of the germination, via the further measurement of the length of the aerial part and the roots and the number of axillary buds for nine weeks, showed that the MS basal medium was more efficient than media 1/2M.S and WPM. Among the eight tested hormones, IAA yielded the best growth of the plantlets. Hormonal combination of NAA and kinetin resulted into a per-centage of the greatest success in reaching 67 % micropropagation. The study also revealed that the MS basal medium in the presence of the IAA plants can acclimate most easily in two types of substrates with improved development in the peat alone.

scholarly journals EVALUATION OF VARIOUS NATURAL DIETS FOR MASS REARING OF Spodoptera frugiperda J.E SMITH (LEPIDOPTERA: NOCTUIDAE)

2021 ◽  
Vol 21 (1) ◽  
pp. 43-48
Author(s):  
Sempurna Ginting ◽  
Tri Sunardi ◽  
Chaincin Buana Sari ◽  
Risky Hadi Wibowo
Keyword(s):  
Life Cycle ◽  
Spodoptera Frugiperda ◽  
Sugar Cane ◽  
Growth And Development ◽  
Mass Rearing ◽  
Cycle Period ◽  
Water Spinach ◽  
Potato Leaf ◽  
Lepidoptera Noctuidae

Evaluation of various natural diets for mass rearing of Spodoptera frugiperda J.E Smith (Lepidoptera: Noctuidae). Spodoptera frugiperda is one of the pests that attack corn in Indonesia. This study aimed to evaluate the most suitable diet for rearing of S. frugiperda from various natural diets. The study was conducted in vitro. The treatments were consisted of variation on S. frugiperda natural diets, such as maize leaf, green mustard leaf, water spinach, sweet potato leaf, sugar cane leaf, and soybeans leaf. The observed variables were life cycle period, pupa size, and pupa weight. The results showed that the shortest life cycle period was on corn leaves diet (40.92 days), and the longest was on sugarcane leaves (45.01 days). The longest size of pupa were S. frugiperda on mustard leaves diet (12.86 mm) and corn leaves (12.56 mm), The heaviest pupa weights were observed in S. frugiperda on mustard leaves diet (0.18 mg), and corn leaves (0.16 mg). Based on the data, it could be concluded that corn leaves were the most suitable type of diet for the growth and development of S. frugiperda.

scholarly journals Enhanced Seed Germination and Seedling Growth of Tillandsia Eizii In Vitro

HortScience ◽  
2003 ◽  
Vol 38 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Kimberly A. Pickens ◽  
James M. Affolter ◽  
Hazel Y. Wetzstein ◽  
Jan H.D. Wolf
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
Seedling Growth ◽  
Culture Medium ◽  
Habitat Destruction ◽  
Light Conditions ◽  
Physiological Constraints ◽  
Large Numbers ◽  
In The Wild

Tillandsia eizii is an epiphytic bromeliad that due to over-collection, habitat destruction, and physiological constraints has declined to near threatened status. This species exhibits high mortality in the wild, and seed are characterized by low percentages of germination. As a means to conserve this species, in vitro culture protocols were developed to enhance seed germination and seedling growth. A sterilization protocol using 70% ethanol for 2 minutes followed by 2.6% NaOCl for 40 minutes disinfested seed and promoted seedling growth. Sucrose incorporated into the culture medium had no effect on germination or growth, while NAA inhibited growth, but not germination. Cultures maintained under a 16-hour photoperiod at 22 °C exhibited greater growth than those grown at 30 °C. Seed that germinated in the dark remained etiolated and failed to develop even after transfer to light conditions. Plants grown in vitro were successfully acclimatized and transferred to the greenhouse. Over 86% survival and rapid growth were obtained with either an all-pine-bark medium, or a mixture of 2 redwood bark: 2 fir bark: 2 potting mix: 1 perlite. This demonstrated that in vitro culture of seed may be used to rapidly produce large numbers of T. eizii, and thus can be used for the conservation and reintroduction of this species.

Use of immature seed germination technique as an alternative to in vitro culture of sunflower (Helianthus annuus L.) embryos

Euphytica ◽  
1996 ◽  
Vol 91 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Adriana Torresán ◽  
Juan Kesteloot ◽  
Fernando Castaño ◽  
Raúl Rodríguez ◽  
Mabel Colabelli
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
Helianthus Annuus ◽  
Immature Seed ◽  
Helianthus Annuus L

scholarly journals In vitro seed germination and Growth of Three Varieties of Black Gram after Ultraviolet-B Radiation

2016 ◽  
Vol 4 (1) ◽  
pp. 117-129 ◽  
Author(s):  
K Rajendiran ◽  
K Thiruvarasan ◽  
R Vijayalakshmi
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
Plant Biomass ◽  
Shoot Length ◽  
Ultraviolet B ◽  
Black Gram ◽  
Root And Shoot Length ◽  
Dry And Wet Conditions ◽  
Germination And Growth

In vitro seed germination and growth of seedlings was tried with three varieties of black gram (Vigna mungo (L.) Hepper) viz. Vamban-3, Nirmal-7 and T-9 after ultraviolet-B irradiation (UV-B = 2 hours once with 1 hour recovery time @ 12.2 kJ m-2 d-1; ambient = 10 kJ m-2 d-1). Unstressed and UV-B stressed VAMBAN-3 and T-9 seeds both in dry and wet conditions responded to in vitro germination. Unstressed NIRMAL-7 failed to germinate under in vitro culture. UV-B stressed NIRMAL-7 responded to in vitro culture. UV-B irradiation enhanced seedling height at both dry and wet conditions in VAMBAN-3 followed by NIRMAL-7 compared with controls. Root and shoot length of UV-B stressed VAMBAN-3 and NIRMAL-7 performed five to six times better than control. Root and shoot length of T-9 was reduced (2.61 to 8.69 %) below control after UV-B exposure. UV-B stressed VAMBAN-3 under dry and wet exposure accumulated three to six times more plant biomass over controls. UV-B stressed NIRMAL-7 and T-9 dry seeds accumulated less plant biomass by 70.86 % and 12.39 % respectively than their controls. NIRMAL-7 and T-9 under dry UV-B exposure produced two times more leaves than control.Int J Appl Sci Biotechnol, Vol 4(1): 117-129

scholarly journals The photoperiodic control of growth and development of Chenopodium rubrum L. plants in vitro

2007 ◽  
Vol 59 (3) ◽  
pp. 203-208 ◽  
Author(s):  
Aleksandra Mitrovic ◽  
Z. Giba ◽  
Ljubinka Culafic
Keyword(s):  
Life Cycle ◽  
Plant Growth ◽  
Seed Development ◽  
Growth And Development ◽  
Reproductive Development ◽  
Day Length ◽  
Chenopodium Rubrum ◽  
Development In Vitro ◽  
Photoperiod Sensitive

Influence of the photoperiod on growth, flowering, and seed development in vitro of Chenopodium rubrum L., a short day annual, was examined. Chenopodium rubrum plants modify their growth and reproductive development in accordance with the photoperiod. With an increase of day length, growth was stimulated, flowering was delayed, seed development occurred earlier, and the plants produced more seeds. By altering photoperiods during induction and evocation of flowering, it is shown that the photoperiod experienced by seedlings during early reproductive development determines the pattern of plant growth to the end of ontogenesis, the time to flowering, and the course of seed development. It is therefore concluded that growth and reproductive development of C. rubrum are photoperiod-sensitive to during a precise short part of its life cycle. .

scholarly journals How Carbon Source and Seedcoat Influence the In Vitro Culture of Peach (Prunus persica L. Batsch) Immature Seeds

HortScience ◽  
2021 ◽  
pp. 1-2
Author(s):  
Margarita Pérez-Jiménez ◽  
Alfonso Guevara-Gázquez ◽  
Antonio Carrillo-Navarro ◽  
José Cos-Terrer
Keyword(s):  
Seed Germination ◽  
Carbon Source ◽  
In Vitro Culture ◽  
Seedling Growth ◽  
Woody Plant ◽  
Prunus Persica ◽  
In Vitro Germination ◽  
Immature Seeds ◽  
Woody Plant Medium

The effects of carbon source and concentration and of seedcoat were tested on the in vitro germination of peach seeds derived from crosses performed in the field. Seeds were extracted from the fruit and cultured in Woody Plant Medium (WPM) supplemented with sucrose, glucose, or sorbitol at concentrations of 15, 30, and 45 g·L−1. The percentage of germination as well as the root and hypocotyl lengths were measured after the stratification process and before acclimatization. Seedcoat did not have any influence on seed germination in any tested media and genotype. Glucose at a concentration of 15 g·L−1 and sucrose at 15, 30, and 45 g·L−1 resulted in greater stem seedling growth. The root developed the most when seeds were cultured in media with 15 or 30 g·L−1 of sucrose.

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