scholarly journals EVALUATION OF VARIOUS NATURAL DIETS FOR MASS REARING OF Spodoptera frugiperda J.E SMITH (LEPIDOPTERA: NOCTUIDAE)

2021 ◽  
Vol 21 (1) ◽  
pp. 43-48
Author(s):  
Sempurna Ginting ◽  
Tri Sunardi ◽  
Chaincin Buana Sari ◽  
Risky Hadi Wibowo

Evaluation of various natural diets for mass rearing of Spodoptera frugiperda J.E Smith (Lepidoptera: Noctuidae). Spodoptera frugiperda is one of the pests that attack corn in Indonesia. This study aimed to evaluate the most suitable diet for rearing of S. frugiperda from various natural diets. The study was conducted in vitro. The treatments were consisted of variation on S. frugiperda natural diets, such as maize leaf, green mustard leaf, water spinach, sweet potato leaf, sugar cane leaf, and soybeans leaf. The observed variables were life cycle period, pupa size, and pupa weight. The results showed that the shortest life cycle period was on corn leaves diet (40.92 days), and the longest was on sugarcane leaves (45.01 days). The longest size of pupa were S. frugiperda on mustard leaves diet (12.86 mm) and corn leaves (12.56 mm), The heaviest pupa weights were observed in S. frugiperda on mustard leaves diet (0.18 mg), and corn leaves (0.16 mg). Based on the data, it could be concluded that corn leaves were the most suitable type of diet for the growth and development of S. frugiperda.

2021 ◽  
Vol 21 (1) ◽  
pp. 43-48
Author(s):  
Sempurna Ginting ◽  
Tri Sunardi ◽  
Chaincin Buana Sari ◽  
Risky Hadi Wibowo

Evaluation of various natural diets for mass rearing of Spodoptera frugiperda J.E Smith (Lepidoptera: Noctuidae).Spodoptera frugiperda is one of the pests that attack corn in Indonesia. This study aimed to evaluate the most suitable dietfor rearing of S. frugiperda from various natural diets. The study was conducted in vitro. The treatments were consisted ofvariation on S. frugiperda natural diets, such as maize leaf, green mustard leaf, water spinach, sweet potato leaf, sugar caneleaf, and soybeans leaf. The observed variables were life cycle period, pupa size, and pupa weight. The results showed thatthe shortest life cycle period was on corn leaves diet (40.92 days), and the longest was on sugarcane leaves (45.01 days). Thelongest size of pupa were S. frugiperda on mustard leaves diet (12.86 mm) and corn leaves (12.56 mm), The heaviest pupaweights were observed in S. frugiperda on mustard leaves diet (0.18 mg), and corn leaves (0.16 mg). Based on the data, it couldbe concluded that corn leaves were the most suitable type of diet for the growth and development of S. frugiperda.


2019 ◽  
Vol 11 (4) ◽  
pp. 126
Author(s):  
Lauren M. Barcelos ◽  
Fabrício O. Fernandes ◽  
Caroline Lopes ◽  
Beatriz M. Emygdio ◽  
Ricardo Valgas ◽  
...  

Saccharine sorghum has been analyzed as a supplementary prime matter for ethanol production, especially during the sugarcane off-season period. However, it has proven to be highly susceptible to insect attacks during the cultivation cycle. The fall armyworm should be emphasized due to its voracity and high damage capacity enhanced by feeding-caused decrease in photosynthetic area. Current analysis studies the biology and determines the nutritional indexes of Spodoptera frugiperda in saccharine sorghum. Cultivars of saccharine sorghum BRS 506, BRS 509 and BRS 511were evaluated. Duration and survival of the egg, caterpillar, pre-pupal and pupal phases were determined, coupled to weight of pupae and caterpillar, life span, fecundity and pre-egg laying period. Although S. frugiperda completed its life cycle on cultivars BRS 506 and BRS 511, egg-laying and egg feasibility rates were low, whereas insects did not lay eggs on cultivar BRS 509. There was no significant difference in feeding intake by S. frugiperda among these three sorghum cultivars. Results suggest that saccharine sorghum is not a suitable host for S. frugiperda. Biological data reveal that the three saccharine sorghum cultivars are recommended for the grain production system since the number of specimens of the next generation is low or null.


Plant Disease ◽  
2003 ◽  
Vol 87 (11) ◽  
pp. 1283-1289 ◽  
Author(s):  
J. M. Stein ◽  
W. W. Kirk

The sensitivities of 11 isolates of Phytophthora infestans to dimethomorph were examined at all stages of the asexual life cycle and when inoculated onto potato leaf discs. In vitro zoospore encystment and cyst germination were highly sensitive to dimethomorph with 50% reduction of mycelial growth and cyst germination (EC50) values for most isolates <0.20 μg/ml, whereas direct sporangia germination and in vitro hyphal growth and sporulation were less sensitive (means of 0.45 and 0.22 μg/ml, respectively). Zoosporogenesis was not significantly inhibited at the maximum dimethomorph concentration examined, 10 μg/ml. Significant differences (Fisher's least significant difference, P = 0.05) in the EC50 values were present between isolates for all stages of the asexual life cycle, except direct sporangia germination and zoosporogenesis. Sensitivity ratios between the least- and most-sensitive isolates were 6.11, 12.14, 12.36, and 10.56 for hyphal growth, in vitro sporulation, zoospore encystment, and cyst germination, respectively. Application of dimethomorph at 1,000 μg/ml to potato leaf discs at 24 or 48 h before inoculation completely inhibited symptom incidence for most isolates, whereas application after inoculation generally was not significantly different from the untreated control, regardless of concentration. Sporulation from leaf discs treated with dimethomorph at 24 or 48 h after inoculation was completely inhibited for all isolates with dimethomorph at 1,000 μg/ml, even when symptom incidence was not significantly reduced.


2016 ◽  
Vol 69 (1) ◽  
pp. 7763-7771 ◽  
Author(s):  
Ana Milena Castro Marquez ◽  
Daniel Rodriguez Caicedo

This study describes the life cycle of Copitarsia uncilata Burgos & Leiva (Lepidoptera: Noctuidae) under laboratory conditions without photophase and a second experiment with photophase of 12 hours on three natural diets. The life cycle of C. uncilata was significantly shorter for females (76.46 ± 1.01 days, p=0.033) reared on alstroemeria (Alstroemeria sp.) diet without photophase, and for males (79.78 ± 0.36 days, p=0.046) reared on broccoli (Brassica oleracea italica), with photophase. The emergence of the adults was 100% and 73.33% from larvae fed on alstroemeria, 90.9% and 88.88% for individuals fed on broccoli, 86.2% and 50% for those fed on cauliflower (Brassica oleracea var. botrytis), without and with photophase respectively. The sex ratio (male:female) of individuals reared without photophase, evidenced a higher rate of females on alstroemeria (1:1.3), followed by cauliflower (1:0.6) and broccoli (1:0.5). In the experiment with photophase, the sex ratio was higher on alstroemeria (1:1.5), followed by cauliflower (1:0.9) and broccoli (1:0.6). As a conclusion, the most suitable diet for laboratory mass rearing in terms of life cycle parameters of C. uncilata is broccoli followed by alstroemeria and cauliflower.


2007 ◽  
Vol 59 (3) ◽  
pp. 203-208 ◽  
Author(s):  
Aleksandra Mitrovic ◽  
Z. Giba ◽  
Ljubinka Culafic

Influence of the photoperiod on growth, flowering, and seed development in vitro of Chenopodium rubrum L., a short day annual, was examined. Chenopodium rubrum plants modify their growth and reproductive development in accordance with the photoperiod. With an increase of day length, growth was stimulated, flowering was delayed, seed development occurred earlier, and the plants produced more seeds. By altering photoperiods during induction and evocation of flowering, it is shown that the photoperiod experienced by seedlings during early reproductive development determines the pattern of plant growth to the end of ontogenesis, the time to flowering, and the course of seed development. It is therefore concluded that growth and reproductive development of C. rubrum are photoperiod-sensitive to during a precise short part of its life cycle. .


2021 ◽  
Author(s):  
Lin Xu ◽  
Xiao‐Long Meng ◽  
Saqib Hussain Bangash ◽  
Fan Zhang ◽  
Dong‐Qiang Zeng ◽  
...  

Biologia ◽  
2015 ◽  
Vol 70 (4) ◽  
Author(s):  
Darima Habib ◽  
Muhammad Zia ◽  
Yamin Bibi ◽  
Bilal Haider Abbasi ◽  
Muhammad Fayyaz Chaudhary

AbstractNitrogen assimilating enzymes play curtail role during un-differentiation and re-differentiation of plant cells. To investigate role and pattern of glutamine synthetase (GS), nitrate reductase (NR) and glutamate dehydrogenase (GDH) during in vitro life cycle of Argyrolobium roseum this study was conducted. The concentrations of these enzymes were determined during seed germination; callus induction from leaf, stem and root explants; shoot regeneration from callus; root development and acclimatization stages. GS and NR enzymes showed ascending pattern during in vitro plant development from seed while GDH concentration decreased during this process. Completely reverse pattern was showed by these enzymes during callogenesis and proliferation phase. Increase in GS and NR activities was noticed in regenerated leaves and stem during shoots and roots developmental phases; and vice verse for GDH. The acclimatization stress also up lifted NR and GS activities in leaf, stem and root tissues. This study highlights the importance of nitrogen assimilating enzymes (NR, GS, and GDH) during growth and development of A. roseum in vitro culture.


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