Distribution of telocytes in the corpus and cervix of human uterus: an immunohistochemical study

Biologia ◽  
2017 ◽  
Vol 72 (10) ◽  
Author(s):  
Martin Klein ◽  
Ladislav Urban ◽  
Ivan Deckov ◽  
Lubos Danisovic ◽  
Stefan Polak ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Morphological Study ◽  
Immunohistochemical Study ◽  
Interstitial Cells ◽  
Human Uterus ◽  
Cd34 Antigen ◽  
Immunohistochemical Methods ◽  
Tissue Specimens ◽  
Immunohistochemical Identification ◽  
Uterine Body

AbstractOver the last few years, researchers have been studying telocytes, recently described interstitial cells, voraciously. This morphological study focused on the immunohistochemical identification of telocytes and the study of their topographical relations in the wall of the human uterine body and cervix. This study attempted to find the most specific and therefore, the most suitable monoclonal antibody for the study of telocytes via the immunohistochemical methods. Tissue specimens from human uteruses were stained with eight different primary antibodies, to detect the expression of c-kit (CD117), CD34 antigen, vimentin,

scholarly journals Primitive hematopoietic cells in murine bone marrow express the CD34 antigen

Blood ◽  
1996 ◽  
Vol 88 (10) ◽  
pp. 3774-3784 ◽  
Author(s):  
F Morel ◽  
SJ Szilvassy ◽  
M Travis ◽  
B Chen ◽  
A Galy
Keyword(s):  
Stem Cells ◽  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Significant Proportion ◽  
Hematopoietic Cells ◽  
Full Detail ◽  
Hematopoietic Stem ◽  
Cd34 Antigen

The CD34 antigen is expressed on most, if not all, human hematopoietic stem cells (HSCs) and hematopoietic progenitor cells, and its use for the enrichment of HSCs with repopulating potential is well established. However, despite homology between human and murine CD34, its expression on subsets of primitive murine hematopoietic cells has not been examined in full detail. To address this issue, we used a novel monoclonal antibody against murine CD34 (RAM34) to fractionate bone marrow (BM) cells that were then assayed in vitro and in vivo with respect to differing functional properties. A total of 4% to 17% of murine BM cells expressed CD34 at intermediate to high levels, representing a marked improvement over the resolution obtained with previously described polyclonal anti-CD34 antibodies. Sixty percent of CD34+ BM cells lacked lineage (Lin) markers expressed on mature lymphoid or myeloid cells. Eighty-five percent of Sca-1+Thy-1(10)Lin- /10 cells that are highly enriched in HSCs expressed intermediate, but not high, levels of CD34 antigen. The remainder of these phenotypically defined stem cells were CD34-. In vitro colony-forming cells, day-8 and -12 spleen colony-forming units (CFU-S), primitive progenitors able to differentiate into B lymphocytes in vitro or into T lymphocytes in SCID mice, and stem cells with radioprotective and competitive long-term repopulating activity were all markedly enriched in the CD34+ fraction after single-parameter cell sorting. In contrast, CD34-BM cells were depleted of such activities at the cell doses tested and were capable of only short-term B-cell production in vitro. The results indicate that a significant proportion of murine HSCs and multilineage progenitor cells express detectable levels of CD34, and that the RAM34 monoclonal antibody is a useful tool to subset primitive murine hematopoietic cells. These findings should facilitate more direct comparisons of the biology of CD34+ murine and human stem and progenitor cells.

scholarly journals Immunoistochemical and morphological study of periodont tissues when predicting the results of dental implantation in patients with chronic parodontitis

2019 ◽  
Vol 488 (4) ◽  
pp. 452-456
Author(s):  
A. A. Kulakov ◽  
E. A. Kogan ◽  
T. V. Brailovskaya ◽  
A. P. Vedyaeva ◽  
N. V. Zharkov
Keyword(s):  
Proliferative Activity ◽  
Morphological Study ◽  
Immunohistochemical Study ◽  
Additional Treatment ◽  
Paraffin Sections ◽  
Regenerative Capacity ◽  
Ki 67 ◽  
Dental Implantation ◽  
Growth Zones ◽  
Severe Degree

A morphological and immunohistochemical study of 24 gums biopsies was conducted in 19 patients aged 35-60 years with a diagnosis of partial secondary edentulous, chronic generalized periodontitis of moderate and severe degree (14 patients), and also without pathological changes in the periodontal disease (5 patients), who underwent dental implantation. Immunohistochemical reactions with antibodies to Ki-67, VEGF, SMA were performed on serial paraffin sections. It has been established that chronic periodontitis is characterized by a higher proliferative activity of the epithelium, which reflects its hyperplastic changes, as well as a lower content of SMA positive cells and the practical absence of the formation of privascular couplings from SMA-positive cells that are associated in tissues with growth zones, which indirectly indicates reduced tissue regenerative capacity. Therefore, in the case of the operation of dental implantation requires additional treatment aimed at anti-inflammatory and pro-regenerative effects.

scholarly journals MONOCLONAL ANTIBODY TO DESMOSOME ASSOCIATED 34kD PROTEIN AND IMMUNOHISTOCHEMICAL STUDY IN RELATION TO THE MALIGNANCY OF TRANSITIONAL CELL CARCINOMAS OF URINARY BLADDER

1989 ◽  
Vol 80 (12) ◽  
pp. 1769-1775
Author(s):  
Atushi Takenaka ◽  
Sohei Kitazawa ◽  
Takashi Koyama ◽  
Takahiro Fujimori ◽  
Akinobu Gotoh ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Urinary Bladder ◽  
Immunohistochemical Study ◽  
Transitional Cell

IMMUNOHISTOCHEMICAL IDENTIFICATION OF PENICILLIUM MARNEFFEI BY MONOCLONAL ANTIBODY

1992 ◽  
Vol 31 (6) ◽  
pp. 410-412 ◽  
Author(s):  
JORGE ARRESE ESTRADA ◽  
DIRK STYNEN ◽  
JAN CUTSEM ◽  
CLAUDINE PIÉRARD-FRANCHIMONT ◽  
GERALD E. PIÉRARD
Keyword(s):  
Monoclonal Antibody ◽  
Penicillium Marneffei ◽  
Immunohistochemical Identification

The Role of Neurogenic Bioamines in Nerve Fibers of Uterus during the Postpartum Involution in Experimental Animal Models

2021 ◽  
Vol 27 ◽  
Author(s):  
Sergey V. Dindyaev ◽  
Narasimha M Beeraka ◽  
Denis V. Kasatkin ◽  
Elizaveta V. Mikhaylenko ◽  
Siva G. Somasundaram ◽  
...  
Keyword(s):  
Glyoxylic Acid ◽  
Sympathetic Nerves ◽  
Biological Action ◽  
Nerve Fibers ◽  
Structural Elements ◽  
Rat Uterus ◽  
Postpartum Involution ◽  
Tissue Specimens ◽  
Nulliparous Female ◽  
Uterine Body

Background: Biogenic amines (BAs) secreted by the sympathetic neural apparatus of rat uterus is reported to be conducive to the uterine functional activity during postpartum involution; the imbalance in BAs ratio could confer postpartum reproductive disorders including improper postpartum involution. Objective: The changes in density of uterine sympathetic nerves implicated in the pathology of endometriosis, adenomyosis, and delayed uterine involution. The present study is aimed to ascertain ‘serotonin’ and ‘catecholamine’ concentrations in mesenteric mast cells (MCs), and structural elements of nerve fibers across the perivascular plexuses (PPs) and single sympathetic nerve terminals (SST). Methods: Furthermore, the density of their spatial distribution (SDP and SDT) in the uterine body, cervix, and mesometrium was determined during postpartum involution. Tissue specimens of postpartum uterus were obtained from 55 nulliparous female Wistar outbred strain rats, which were grouped according to the days after parturition at the time of sacrifice. The nerve fibers of PP and SST exhibited emerald green fluorescence, which was detected by glyoxylic acid fluorescence technique; the fluorescence invoked by BAs was identified by microspectrofluorimetry. Results: Concentrations of BAs were extensive in the varicosities of PP and SST on the 10th day. However, the highest BA concentrations were found in structural elements of PP in the uterine mesometrium in the initial days of postpartum. In mesenteric MC, serotonin and catecholamines were at the highest concentration on 10th day postpartum. Histamines peaked on the 6th day. Conclusion: SDP and SDT were increased significantly in all structural elements of uterine nerve fibers in the uterine body and cervix compared to SDP in mesentery. Considering that catecholamines and serotonin are antagonists in many aspects of their biological action, the ratio of BAs should be well-balanced to maintain anabolic-catabolic equilibrium in the rat uterus.

scholarly journals Immunohistochemical identification of resistin in the uterus of ewes subjected to different diets: Preliminary results

2019 ◽  
Vol 63 (2) ◽  
Author(s):  
Cecilia Dall'Aglio ◽  
Paola Scocco ◽  
Margherita Maranesi ◽  
Linda Petrucci ◽  
Gabriele Acuti ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Adipose Tissue ◽  
Nutritional Status ◽  
Reproductive Activity ◽  
Epithelial Lining ◽  
Male And Female ◽  
Endogenous Production ◽  
Uterine Glands ◽  
Ovine Uterus ◽  
Immunohistochemical Identification

Resistin is a polypeptide hormone of the adipokine-family, primarily, but not exclusively, produced by the adipose tissue. Recent studies suggested that resistin may affect the male and female reproductive activity. The study aim was to immunohistochemically evaluate the presence and distribution of resistin in the ovine uterus. Uterine samples were collected from two groups of ewes at the end of an experimental trial during which the animals of the first group (CTRL) were fed only by grazing while those of the second one (EXP) were supplemented with barley and corn. Using a monoclonal antibody against resistin, tested by Western Blot, the immunopositive reaction was identified in the cytoplasm of epithelial lining cells and uterine glands. The endogenous production of resistin seemed to be affected by different diet, as evidenced by staining differences between the CTRL and EXP groups. Our findings support the existence of a peripheral resistin system in the sheep uterus. It is possible that this system is involved in the functionality of the uterus, which is also affected by the animal’s nutritional status. 

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