scholarly journals Monitoring of Therapeutic Serum Concentrations of Antiepileptic Drugs by a Newly Developed Gas Chromatographic Procedure and Enzyme Immunoassay (EMIT): A Comparative Study

1981 ◽  
Vol 19 (5) ◽  
Author(s):  
W. R. Külpmann ◽  
M. Oellerich
Keyword(s):  
Comparative Study ◽  
Enzyme Immunoassay ◽  
Antiepileptic Drugs ◽  
Serum Concentrations ◽  
Chromatographic Procedure

scholarly journals Salivary Theophylline Concentrations in Neonates

1994 ◽  
Vol 31 (3) ◽  
pp. 277-280
Author(s):  
Susan J Thompson
Keyword(s):  
Enzyme Immunoassay ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Serum Concentrations ◽  
Serum Theophylline ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic ◽  
Free Serum ◽  
Homogeneous Enzyme Immunoassay

Salivary theophylline concentrations in neonates were measured by a homogeneous enzyme immunoassay and compared with serum concentrations. Free serum theophylline concentrations were also compared with salivary concentrations and the values obtained were almost identical. Results using the immunoassay agreed well with those obtained using a high-performance liquid chromatographic procedure (HPLC).

Enzymatic Immunoassay vs. Gas/Liquid Chromatography for Determination of Phenobarbital and Diphenylhydantoin in Serum

1975 ◽  
Vol 21 (12) ◽  
pp. 1766-1768 ◽  
Author(s):  
Harold E Booker ◽  
Barbara A Darcey
Keyword(s):  
Liquid Chromatography ◽  
Enzyme Immunoassay ◽  
Antiepileptic Drugs ◽  
False Positives ◽  
Large Series ◽  
Gas Liquid Chromatography ◽  
False Negatives ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic

Abstract The antiepileptic drugs diphenylhydantoin and phenobarbital were measured in serum by a new commercially available enzyme immunoassay procedure ("EMIT," Syva Corp.). The procedure requires <5 min and no more than 50 µl of serum per determination. It is simple; only four steps (pipetting and diluting with an automatic pipettor-dilutor) are required before spectrophotometry. Twenty replicate analyses of a serum containing phenobarbital and diphenylhydantoin gave results with a CV of 6.8% and 9.1%, respectively. Results attained in a large series of patients were compared with results by a gas/liquid chromatographic procedure. For phenobarbital r = 0.97, and for diphenylhydantoin r = 0.98. No false negatives or false positives were encountered.

scholarly journals Serum concentrations of antidepressants, antipsychotics, and antiepileptics over the bariatric surgery procedure

2021 ◽  
Author(s):  
Susanna M. Wallerstedt ◽  
Karin Nylén ◽  
Magnus A. B. Axelsson
Keyword(s):  
Bariatric Surgery ◽  
Antiepileptic Drugs ◽  
Therapeutic Drug ◽  
Serum Concentrations ◽  
Consistent Finding ◽  
Low Calorie ◽  
Post Surgery ◽  
Low Calorie Diet ◽  
Calorie Diet ◽  
The Impact

Abstract Purpose As a substantial proportion of bariatric surgery patients use psychotropic/antiepileptic drugs, we investigated the impact of this procedure on serum concentrations. Methods In a naturalistic, longitudinal, prospective case series, we compared dose-adjusted trough concentrations of antidepressants, antipsychotics, or antiepileptics in consecutive patients before and after bariatric surgery. Adherence to treatment over 2 weeks preceding each sampling was considered. Results In all, 85 participants were included (86% female, median age 45 years, median body mass index 42 kg/m2). They were being treated with 18 different psychotropic/antiepileptic drugs (7 substances: 6–17 individuals, 11 substances: 1–4 individuals) and contributed 237 samples over a median of 379 days after surgery. For four out of seven substances with pre-/post-surgery samples available from six or more individuals, the dose-adjusted concentration was reduced (sertraline: 51%, mirtazapine: 41%, duloxetine: 35%, citalopram: 19%). For sertraline and mirtazapine, the low-calorie-diet before surgery entirely explained this reduction. A consistent finding, irrespective of drug, was the association between the mean ratio of the post-/pre-diet dose-adjusted concentration and the lipophilicity of the drug (logD; correlation coefficient: −0.69, P = 0.0005), the low-calorie diet often affecting serum concentration more than the surgery itself. Conclusions Serum concentrations of psychotropic/antiepileptic drugs vary after bariatric surgery and can be hard to predict in individual patients, suggesting that therapeutic drug monitoring is of value. Conversely, effects of the pre-surgery, low-calorie diet appear generalizable, with decreased concentrations of highly lipophilic drugs and increased concentrations of highly hydrophilic drugs. Interaction effects (surgery/dose/concentration) were not evident but cannot be excluded.

scholarly journals The influence of concomitant antiepileptic drugs on lamotrigine serum concentrations in Northwest Chinese Han population with epilepsy

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0210600 ◽  
Author(s):  
Xiaonian Han ◽  
Jing Huang ◽  
Jianhua Lv ◽  
Li Ma ◽  
Lirong Peng ◽  
...  
Keyword(s):  
Antiepileptic Drugs ◽  
Chinese Han Population ◽  
Serum Concentrations ◽  
Chinese Han ◽  
Han Population

scholarly journals Studies on viomycin. XV. Comparative study on the specificities of two anti-viomycin antisera by enzyme immunoassay.

1982 ◽  
Vol 30 (7) ◽  
pp. 2487-2491 ◽  
Author(s):  
TSUNEHIRO KITAGAWA ◽  
HIDEAKI TANIMORI ◽  
KENSEI YOSHIDA ◽  
HISATSUGU ASADA ◽  
TAKAKO MIURA ◽  
...  
Keyword(s):  
Comparative Study ◽  
Enzyme Immunoassay

scholarly journals Comparative Study of the Pharmacokinetics of Inorganic and Organic Iron Compounds in Broiler Chickens

2014 ◽  
Vol 37 (1) ◽  
pp. 75-81
Author(s):  
Dimitrichka Dimitrova ◽  
Anna Arnaudova-Matey ◽  
Petar Dilov ◽  
Geno Angelov ◽  
Tandju Mehmedov ◽  
...  
Keyword(s):  
Comparative Study ◽  
Intravenous Injection ◽  
Ferrous Sulfate ◽  
Broiler Chickens ◽  
Alimentary Tract ◽  
Ferrous Sulphate ◽  
Compartmental Analysis ◽  
Serum Concentrations ◽  
Iron Compounds ◽  
Silicone Tube

Abstract The pharmacokinetics of ferrous methionate and ferrous sulphate was investigated in broiler chickens after intravenous injection and crop intubation. The iron compounds were injected intravenously in v. brachialis. After a 20-day “wash-out” period the ferrous methionate and ferrous sulphate were administered again by an elastic silicone tube into the crop. The serum concentrations of the iron were determined using a bioanalyser. Two pharmacokinetic approaches were used - compartmental and non-compartmental analysis. After i.v. injection we found statistically significantly longer and better distribution of the iron contained in the ferrous methionate compared to the ferrous sulphate. The АUC0→∞ was statistically significantly higher in the ferrous methionate. In the alimentary tract of broiler chickens, ferrous methionate was absorbed more rapidly than ferrous sulfate. It was also distributed at a higher volume as compared to the ferrous sulfate

Avidin-Biotin Enzyme Immunoassay of Osteocalcin in Serum or Plasma

1992 ◽  
Vol 38 (10) ◽  
pp. 1968-1974 ◽  
Author(s):  
J Jaouhari ◽  
F Schiele ◽  
S Dragacci ◽  
P Tarallo ◽  
J P Siest ◽  
...  
Keyword(s):  
Human Serum ◽  
Enzyme Immunoassay ◽  
Healthy Subjects ◽  
Limit Of Detection ◽  
Serum Concentrations ◽  
Calcium Dependent ◽  
Standard Curve ◽  
Microtiter Plates ◽  
Analytical Recovery ◽  
High Concentrations

Abstract We describe a competitive enzyme immunoassay, the ExtrAvidin-biotin system, for determining osteocalcin in human serum or plasma. Antibodies were raised against bovine osteocalcin. Binding of the antibodies to osteocalcin was calcium-dependent. Limit of detection is 0.07 nmol/L (0.4 microgram/L). The standard curve for method is linear between 0.3 and 17.6 nmol/L (1.9 and 100 micrograms/L). Interassay CV over the range 0.9 to 14.8 nmol/L (5.3 to 84 micrograms/L) is 7.5% to 11.7%. Analytical recovery is 105% +/- 5% (mean +/- SD). The measurement, which is adapted to microtiter plates, requires only 20 microL of serum and 5 h. The coefficient of correlation between the concentrations measured by this method and by a commercially available radioimmunoassay kit (CIS Biointernational) is 0.91. Osteocalcin can be measured in serum or heparinized plasma. Hemolysis (174 mumol/L hemoglobin) reduces osteocalcin concentration by 54%. High concentrations of triglycerides (7 mmol/L) give an overestimation of 63%. Serum concentrations of osteocalcin measured in 130 healthy subjects (ages 15-64 years) and 86 children (ages 4-14 years) were 1.4 +/- 0.8 and 4.0 +/- 1.5 nmol/L (8.1 +/- 4.6 and 22.5 +/- 8.6 micrograms/L), respectively (mean +/- SD).

Comparative study of five antiepileptic drugs on a translational cognitive measure in the rat: relationship to antiepileptic property

2009 ◽  
Vol 207 (4) ◽  
pp. 513-527 ◽  
Author(s):  
Guy A. Higgins ◽  
Nathalie Breysse ◽  
Elijus Undzys ◽  
D. Richard Derksen ◽  
Melanie Jeffrey ◽  
...  
Keyword(s):  
Comparative Study ◽  
Antiepileptic Drugs ◽  
Cognitive Measure
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