Radical-scavenging activity of glutathione, chitin derivatives and their combination‡

AbstractSince chitosan and its amino-, cinnamo- or cinnamo-amino- derivatives are acid-soluble, the effect of acetic acid on hyaluronan (HA) macromolecules degraded by Cu(II) ions and ascorbate was examined to produce reactive oxygen species (ROS). Further, the effects of glutathione (GSH), chitosan and its derivatives, added individually or in combination, on the quenching of ROS and ABTS

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Determination of Total Phenolic, Tannin, Flavonoid Contents and Evaluation of Antioxidant Property of Amaranthus tricolor (L)

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/phyto.v9i6.8184 ◽

2017 ◽

Vol 9 (6) ◽

Cited By ~ 3

Author(s):

Sowjanya Pulipati ◽

P. Srinivasa Babu ◽

U. Naveena ◽

S.K. Rafeeka Parveen ◽

S.K. Sumaya Nausheen ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reactive Oxygen ◽

Antioxidant Property ◽

Total Phenolic ◽

Oxygen Species ◽

Amaranthus Tricolor

Free radicals or reactive oxygen species are involved in various pharmacological conditions. As synthetic antioxidants possess numerous adverse health effects, the medicinal plants possessing antioxidant components can be used to prevent harmful effects of reactive oxygen species. In the present study leaves of Amaranthus tricolor Linn were used to prepare chloroform (CEAT), methanolic (MEAT) and aqueous (AEAT) extracts, analyze the presence of phytochemicals and evaluation of in-vitro antioxidant property. Quantitative determination of phenols, tannins and flavonoids in leaves A.tricolor was carried out using spectrophotometric methods. The antioxidant activity was performed by DPPH, p-NDA radical scavenging methods for different extracts of the plant. The plant species showed that methanolic extract (MEAT) on higher concentration possess better antioxidant potential when compared with reference standard ascorbic acid. The plant extracts exhibited strong antioxidant DPPH radical scavenging activity with the IC50 values 290, 657, 830 and 130μg/ml of MEAT, CEAT, AEAT and ASA respectively. In scavenging hydroxyl radical by p-NDA method the MEAT showed maximum activity, CEAT showed moderate and AEAT showed minimum activity. The strongest antioxidant activity of MEAT could be due to the presence of flavonoids and phenols.

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A Novel Peptide, Nicotinyl–Isoleucine–Valine–Histidine (NA–IVH), Promotes Antioxidant Gene Expression and Wound Healing in HaCaT Cells

Marine Drugs ◽

10.3390/md16080262 ◽

2018 ◽

Vol 16 (8) ◽

pp. 262 ◽

Cited By ~ 3

Author(s):

Dong Son ◽

Dong Yang ◽

Ji Sun ◽

Seul Kim ◽

Namju Kang ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Wound Healing ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reactive Oxygen ◽

Water Soluble ◽

Oxygen Species ◽

Protective Effects ◽

Healing Effect ◽

Wound Healing Effect

Nicotinamide (NA), a water-soluble vitamin B3, has been shown to exert cellular-protective effects against reactive oxygen species (ROS). In order to improve the cellular-protective effects of NA, we synthesized a novel compound, nicotinyl–isoleucine–valine–histidine (NA–IVH), by combining NA with jellyfish peptides’ IVH. In the present study, we examined the cellular-protective effects of the novel synthetic nicotinyl-peptide, NA–IVH. We found that NA–IVH enhances the radical scavenging activity with a robust increase of the nuclear factor (erythroid-derived 2)-like factor (Nrf2) expression in human HaCaT keratinocytes. In addition, NA–IVH protected the cells from hydrogen peroxide (H2O2)-induced cell death. Interestingly, NA–IVH exhibited an improved wound-healing effect in a high glucose condition, possibly through the regulation of reactive oxygen species (ROS). Collectively, our results imply that a novel nicotinyl-peptide, NA–IVH, has a wound-healing effect in a hyperglycemic condition, possibly by modulating excessive ROS.

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Free Radical Scavenging Activity of Newly Designed Sesamol Derivatives

New Journal of Chemistry ◽

10.1039/d1nj02225c ◽

2021 ◽

Author(s):

Laura Maria Castro Gonzalez ◽

Annia Galano ◽

J. Raul Alvarez-Idaboy

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Free Radical ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Chemical Compounds ◽

Free Radical Scavenging ◽

Scavenging Activity ◽

Oxygen Species ◽

Harmful Effects

Chemical compounds with antioxidant behavior are recognized as efficient protectors against the harmful effects of reactive oxygen species (ROS) and other chemicals overproduced due to oxidative stress (OS). Sesamol has...

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Neuroprotective Effect of 1,4-Naphthoquinones in an In Vitro Model of Paraquat and 6-OHDA-Induced Neurotoxicity

International Journal of Molecular Sciences ◽

10.3390/ijms22189933 ◽

2021 ◽

Vol 22 (18) ◽

pp. 9933

Author(s):

Ekaterina Menchinskaya ◽

Ekaterina Chingizova ◽

Evgeny Pislyagin ◽

Galina Likhatskaya ◽

Yuri Sabutski ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Radical Scavenging Activity ◽

Neuronal Cells ◽

Radical Scavenging ◽

Reactive Oxygen ◽

Neuroprotective Activity ◽

Nonspecific Esterase ◽

Oxygen Species ◽

6 Hydroxydopamine

Targeted screening using the MTT cell viability test with a mini-library of natural and synthetic 1,4-naphthoquinones and their derivatives was performed in order to increase the survival of Neuro-2a neuroblastoma cells in in vitro paraquat and 6-hydroxydopamine models of Parkinson’s disease. As a result, 10 compounds were selected that could protect neuronal cells from the cytotoxic effects of both paraquat and 6-hydroxydopamine. The five most active compounds at low concentrations were found to significantly protect the activity of nonspecific esterase from the inhibitory effects of neurotoxins, defend cell biomembranes from lytic destruction in the presence of paraquat and 6-hydroxydopamine, and normalize the cell cycle. The protective effects of these compounds are associated with the suppression of oxidative stress, decreased expression of reactive oxygen species and nitric oxide formation in cells and normalization of mitochondrial function, and restoration of the mitochondrial membrane potential altered by neurotoxins. It was suggested that the neuroprotective activity of the studied 1,4-NQs is attributable to their pronounced antioxidant and free radical scavenging activity and their ability to reduce the amount of reactive oxygen species formed by paraquat and 6-hydroxydopamine action on neuronal cells. The significant correlation between the neuroprotective properties of 1,4-naphthoquinones and Quantitative Structure–Activity Relationship descriptors describing the physicochemical properties of these compounds means that the hydrophobicity, polarity, charge, and shape of the molecules can be of decisive importance in determining the biological activity of studied substances.

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Vesicular Formation of Trans-Ferulic Acid: an Efficient Approach to Improve the Radical Scavenging and Antimicrobial Properties

Journal of Pharmaceutical Innovation ◽

10.1007/s12247-021-09543-8 ◽

2021 ◽

Author(s):

Anahita Rezaeiroshan ◽

Majid Saeedi ◽

Katayoun Morteza-Semnani ◽

Jafar Akbari ◽

Akbar Hedayatizadeh-Omran ◽

...

Keyword(s):

Escherichia Coli ◽

Reactive Oxygen Species ◽

Ferulic Acid ◽

Reactive Oxygen Species Production ◽

Radical Scavenging ◽

Reactive Oxygen ◽

Entrapment Efficiency ◽

The Body ◽

Oxygen Species ◽

Species Production

Abstract Purposes Reactive oxygen species production is harmful to human’s health. The presence of antioxidants in the body may help to diminish reactive oxygen species. Trans-ferulic acid is a good antioxidant, but its low water solubility excludes its utilization. The study aims to explore whether a vesicular drug delivery could be a way to overcome the poor absorption of trans-ferulic acid hence improving its antimicrobial efficiency and antioxidant effect. Methods Niosomal vesicles containing the drug were prepared by film hydration method. The obtained vesicles were investigated in terms of morphology, size, entrapment efficiency, release behavior, cellular cytotoxicity, antioxidant, cellular protection study, and antimicrobial evaluations. Results The optimized niosomal formulation had a particle size of 158.7 nm and entrapment efficiency of 21.64%. The results showed that the optimized formulation containing 25 μM of trans-ferulic acid could enhance the viability of human foreskin fibroblast HFF cell line against reactive oxygen species production. The minimum effective dose of the plain drug and the niosomal formulation against Staphylococcus aurous (ATCC 29213) was 750 µg/mL and 375 µg/mL, respectively, and for Escherichia coli (ATCC 25922), it was 750 µg/mL and 187/5 µg/mL, respectively. The formulation could also improve the minimum bactericidal concentration of the drug in Staphylococcus aurous, Escherichia coli, and Acinobacter baumannii (ATCC 19606). Conclusion These results revealed an improvement in both antibacterial and antioxidant effects of the drug in the niosomal formulation.

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Role of Anti-Inflammatory, Reactive Oxygen Species Scavenging Activity and Nematicidal Properties of Myrtle Berry Seeds on Helminthiasis Treatment

Journal of Medicinal Food ◽

10.1089/jmf.2020.0089 ◽

2020 ◽

Author(s):

Mohamed-Amine Jabri ◽

Soumaya Hajaji ◽

Kaïs Rtibi ◽

Hichem Sebai

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Scavenging Activity ◽

Oxygen Species ◽

Anti Inflammatory

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Scavenging activity of indole compounds against cisplatin-induced reactive oxygen species

Life Sciences ◽

10.1016/j.lfs.2006.09.011 ◽

2006 ◽

Vol 80 (3) ◽

pp. 254-257 ◽

Cited By ~ 29

Author(s):

Junya Fukutomi ◽

Atsushi Fukuda ◽

Satoshi Fukuda ◽

Masayuki Hara ◽

Atsushi Terada ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Reactive Oxygen ◽

Scavenging Activity ◽

Oxygen Species ◽

Indole Compounds

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Increased levels of thiols protect antimony unresponsive Leishmania donovani field isolates against reactive oxygen species generated by trivalent antimony

Parasitology ◽

10.1017/s0031182007003150 ◽

2007 ◽

Vol 134 (12) ◽

pp. 1679-1687 ◽

Cited By ~ 71

Author(s):

G. MANDAL ◽

S. WYLLIE ◽

N. SINGH ◽

S. SUNDAR ◽

A. H. FAIRLAMB ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Leishmania Donovani ◽

Indian Subcontinent ◽

Current Trend ◽

Reactive Oxygen ◽

Clinical Isolates ◽

Ros Generation ◽

Scavenging Activity ◽

Oxygen Species ◽

Field Isolates

SUMMARYThe current trend of antimony (Sb) unresponsiveness in the Indian subcontinent is a major impediment to effective chemotherapy of visceral leishmaniasis (VL). Although contributory mechanisms studied in laboratory-raised Sb-R parasites include an up-regulation of drug efflux pumps and increased thiols, their role in clinical isolates is not yet substantiated. Accordingly, our objectives were to study the contributory role of thiols in the generation of Sb unresponsiveness in clinical isolates. Promastigotes were isolated from VL patients who were either Sb responsive (n=2) or unresponsive (n=3). Levels of thiols as measured by HPLC and flow cytometry showed higher basal levels of thiols and a faster rate of thiol regeneration in Sb unresponsive strains as compared with sensitive strains. The effects of antimony on generation of reactive oxygen species (ROS) in normal and thiol-depleted conditions as also their H2O2 scavenging activity indicated that in unresponsive parasites, Sb-mediated ROS generation was curtailed, which could be reversed by depletion of thiols and was accompanied by a higher H2O2 scavenging activity. Higher levels of thiols in Sb-unresponsive field isolates from patients with VL protect parasites from Sb-mediated oxidative stress, thereby contributing to the antimony resistance phenotype.

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