DNA Marker-Based High-Throughput Identification of Downy Mildew Infected and Non-Infected Sunflower Plants

Helia ◽  
2019 ◽  
Vol 42 (70) ◽  
pp. 37-43
Author(s):  
Anzhella Solodenko

AbstractDeveloping hybrids, resistant to causal pathogen of Downy mildew (Plasmopara halstedii (Farl.) Berl. & de Toni), is one of the critical tasks in sunflower breeding. Molecular markers have advanced breeding practice in the past decades, however there are still unmet needs for reliable high-throughput (HT) selection of the pathogen resistant starting material and differentiation of the plants infected by different pathogens. In this study, we tested the known DNA marker (308 bp fragment from ribosomal DNA of P. halstedii) for detection of pathogen in different tissues of sunflower plant and at different stages of plant development. Specified DNA marker was re-validated in the total DNA, isolated from sporangium as well as from seedlings of infected pathogen resistant/susceptible inbred lines of Ukrainian breeding and 60 F2 crosses. An independent set of field grown plants with unknown resistance to P. halstedii, having symptoms of the bacterial/fungal/viral pathology were used for HT screening and genotypes, infected with downy mildew, were successfully identified. Pathogen appeared to be concentrated in the vessels of sunflower leaves, in contrast to parenchymal tissue. Our study demonstrates an addition to whole seedling inoculation technique of P. halstedii detection, which allows HT identification of the pathogen infected and non-infected sunflower plants.

2018 ◽  
Vol 22 (6) ◽  
pp. 703-707 ◽  
Author(s):  
E. T. Ilnitskaya ◽  
M. V. Makarkina ◽  
S. V. Tokmakov ◽  
L. G. Naumova

Downy mildew is one of the most common fungal diseases of the vine, caused by Plasmopara viticola. An effective way to control the spread of the pathogen is to cultivate resistant varieties. Cultivars of Vitis vinifera, being the basis of high-quality viticulture, practically do not possess genetic resistance to P. viticola, so screening for resistance donors is an important stage in breeding. One of the major resistance loci to downy mildew, the Rpv3 gene, was identified in the genotype of a complex interspecific hybrid of grapes Bianca. Later, it was found that this gene had seven haplotypes of resistance inherited from North American grape species, and that it was possible to identify the allelic status of the gene using DNA-markers UDV305, UDV737. However, only two haplotypes can be combined in one diploid form. To determine the Rpv3 gene in the grape gene pool we, using these markers, studied 35 different genotypes of grapevines, most of which are interspecies cultivars. Three varieties with known allelic status of the Rpv3 gene (Dunavski lazur, Noah, Seyve Villard 12-375) were included in the study as reference genotypes. The genotypes were studied through polymerase chain reaction with separation of amplification products by capillary electrophoresis in automatic genetic analyzer ABI Prism 3130. In the studied grape cultivars DNA marker analysis indentified the Rpv3 gene in sixteen genotypes of interspecific origin, including haplotype Rpv3299-279 found in twelve varieties, Rpv3321-312 – in three, and haplotype Rpv3null-271 – in one variety. Seyve Villard 12-375 turned out to be the donor of resistance gene in the most of the genotypes carrying Rpv3 in this study. The obtained data can be useful in selection of mildew resistant grape varieties and screening for hybridization pairs.


2020 ◽  
pp. 133-139
Author(s):  
Sanatan Ratna ◽  
B Kumar

In the past few decades, there has been lot of focus on the issue of sustainability. This has occurred due to the growing concerns related to climate change and the growing awareness about environmental concerns. Also, the competition at global level has led to the search for the most sustainable route in the industries. The current research work deals with the selection of green supplier in a Nickle coating industry based on certain weighted green attributes. For this purpose, a hybrid tool comprising of Fuzzy AHP (Fuzzy Analytical Hierarchy) and VIKOR (VlseKriterijumska Optimizacija I Kompromisno Resenje) is used. The Fuzzy AHP is used for assigning proper weights to the selected criteria for supplier evaluation, while VIKOR is used for final supplier selection based on the weighted criteria. The three criterions for green supplier selection are, Ecological packaging, Corporate socio-environmental responsibility and Staff Training. The outcome of the integrated model may serve as a steppingstone to other SMEs in different sectors for selecting the most suitable supplier for addressing the sustainability issue.


2019 ◽  
Vol 26 (13) ◽  
pp. 2330-2355 ◽  
Author(s):  
Anutthaman Parthasarathy ◽  
Sasikala K. Anandamma ◽  
Karunakaran A. Kalesh

Peptide therapeutics has made tremendous progress in the past decade. Many of the inherent weaknesses of peptides which hampered their development as therapeutics are now more or less effectively tackled with recent scientific and technological advancements in integrated drug discovery settings. These include recent developments in synthetic organic chemistry, high-throughput recombinant production strategies, highresolution analytical methods, high-throughput screening options, ingenious drug delivery strategies and novel formulation preparations. Here, we will briefly describe the key methodologies and strategies used in the therapeutic peptide development processes with selected examples of the most recent developments in the field. The aim of this review is to highlight the viable options a medicinal chemist may consider in order to improve a specific pharmacological property of interest in a peptide lead entity and thereby rationally assess the therapeutic potential this class of molecules possesses while they are traditionally (and incorrectly) considered ‘undruggable’.


Author(s):  
John Hunsley ◽  
Eric J. Mash

Evidence-based assessment relies on research and theory to inform the selection of constructs to be assessed for a specific assessment purpose, the methods and measures to be used in the assessment, and the manner in which the assessment process unfolds. An evidence-based approach to clinical assessment necessitates the recognition that, even when evidence-based instruments are used, the assessment process is a decision-making task in which hypotheses must be iteratively formulated and tested. In this chapter, we review (a) the progress that has been made in developing an evidence-based approach to clinical assessment in the past decade and (b) the many challenges that lie ahead if clinical assessment is to be truly evidence-based.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Ross M. Lawrence ◽  
Eric W. Bridgeford ◽  
Patrick E. Myers ◽  
Ganesh C. Arvapalli ◽  
Sandhya C. Ramachandran ◽  
...  

AbstractUsing brain atlases to localize regions of interest is a requirement for making neuroscientifically valid statistical inferences. These atlases, represented in volumetric or surface coordinate spaces, can describe brain topology from a variety of perspectives. Although many human brain atlases have circulated the field over the past fifty years, limited effort has been devoted to their standardization. Standardization can facilitate consistency and transparency with respect to orientation, resolution, labeling scheme, file storage format, and coordinate space designation. Our group has worked to consolidate an extensive selection of popular human brain atlases into a single, curated, open-source library, where they are stored following a standardized protocol with accompanying metadata, which can serve as the basis for future atlases. The repository containing the atlases, the specification, as well as relevant transformation functions is available in the neuroparc OSF registered repository or https://github.com/neurodata/neuroparc.


1998 ◽  
Vol 22 ◽  
pp. 120-122
Author(s):  
A. S. Chaudhry

The need to develop an in vitro method to simulate ruminal digestion of protein foods has long been recognized. An in vitro method must be more rapid, consistent and convenient than in sacco methods. Purified enzymes have been examined in the past to estimate in vitro degradability of protein foods (Poos-Floyd et al., 1985; Aufrere et al., 1991; Luchini et al., 1996) or their fractions (Chaudhry and Webster, 1994). However, the selection of an appropriate enzyme for a range of foodstuffs remains to be realized. This study examined the potential of two enzymes and a centrifuged rumen fluid (CRF) to estimate proteolysis of food proteins.


Plant Disease ◽  
1999 ◽  
Vol 83 (5) ◽  
pp. 482-485 ◽  
Author(s):  
Margaret J. Green ◽  
Dan A. Thompson ◽  
Donald J. MacKenzie

A simple and efficient procedure for the extraction of high-quality DNA from phytoplasma-infected woody and herbaceous plants for polymerase chain reaction (PCR) detection is described. This procedure does not require phenol, chloroform, or alcohol for the precipitation of nucleic acids. Herbaceous and woody plant material are extracted in an identical manner with no additional purification or enrichment steps required. The method utilizes commercially available microspin-column matrices, and the extraction of total DNA can be achieved in less than 1 h. The method has been used to successfully purify phytoplasma DNA from whole leaves, leaf petioles and midribs, roots, and dormant wood from a diverse selection of plant material. The phytoplasmas detected by PCR include pear decline, western X-disease, peach yellow leaf roll, peach rosette, apple proliferation, Australian grapevine yellows, and Vaccinium witches'-broom.


1899 ◽  
Vol 45 (191) ◽  
pp. 713-724
Author(s):  
F. Ashby Elkins ◽  
Jas. Middlemass

We think it will be generally acknowledged that the problem which the treatment of noisy, destructive, and dirty patients sets to their medical officers is greatest as regards their management at night. It is then undoubtedly that noise, destructiveness, and dirty habits have the greatest chance of getting free play, and it is then that the efforts for reformation have to be greatest. If these efforts are successful considerably more than half the problem will have been solved. It is to this part of the question, viz. the supervision of such patients during the night, that we desire in this paper to direct attention. At the outset it may be stated that our proposals are not theoretical. They are the result of practical experience gained during the past four years in the Sunderland Asylum. The special arrangements we propose to describe were instituted by one of us at the opening of the institution four years ago. At first a few cases were dealt with tentatively, but, as the first results were so encouraging, the number of cases was gradually increased, until all the patients who were restless, noisy, destructive, or of dirty habits came without exception to be dealt with. The asylum, situated at Ryhope, is a small one, containing only 350 beds, and on this account, as well as because it was new, it was conveniently suited for such an experiment. It may be well before going further to describe the arrangements now in existence there. There are 175 beds for each sex, made up as follows:—45 single rooms, one fully padded, and 2 half-padded; 2 small dormitories of 7 each, 2 of 13 each, 2 of 19 each, and 2 of 26 each. In the last two there is a night attendant, and one also in one of the dormitories for 19, which is the hospital ward. There is, in addition, a head night attendant who visits the patients in these dormitories and also all the remaining patients every hour, or oftener when necessary. There are thus 4 of a night staff for 175 patients. Though this is probably a large proportion compared to most public asylums, it is not claimed as a new departure in asylum management, as we are aware that in a number of asylums the advantage of having a large night staff is fully realised and acted on. The essential feature of the arrangements at Ryhope, to which we wish to direct attention, is the selection of cases placed in dormitories under constant supervision. Of course, all epileptics and suicidal patients are placed there. But, in addition, all recent cases of whatever kind, all dirty and destructive cases, and those who sleep badly and are in consequence inclined to chatter or be noisy, are also placed under constant supervision. Looked at from the other side, all single rooms and dormitories not under constant supervision are reserved for quiet and well-behaved patients who do not require any special attention during the night. This plan has been found to work exceedingly well, and since it was organised we have never had occasion to think of adopting any other. Another testimony to its effectiveness is that those of the staff who have the actual supervision of the patients and have had experience in other asylums are unanimous in their opinion that the arrangement is a very decided improvement. This opinion, let it be observed, is not based on the ground that now their duties are lighter than they were, because, as a matter of fact, they are more onerous.


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