Organosolv pretreatment of olive tree biomass for fermentable sugars

Holzforschung ◽  
2011 ◽  
Vol 65 (2) ◽  
Author(s):  
Manuel J. Díaz ◽  
Wouter J.J. Huijgen ◽  
Ron R. van der Laan ◽  
Johannes H. Reith ◽  
Cristóbal Cara ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aqueous Ethanol ◽  
Sugar Content ◽  
Olive Tree ◽  
Main Process ◽  
Organosolv Pretreatment ◽  
Ethanol Content ◽  
Mediterranean Countries ◽  
Hydrolysis Of ◽  
Tree Pruning

Abstract Olive tree pruning biomass is one of the main agricultural residues available in Mediterranean countries and is currently lacking commercial applications. To take advantage of its sugar content, a pretreatment is necessary to enhance enzyme accessibility of the cellulose fraction of the residue. This paper describes for the first time the use of organosolv pretreatment in this regard. The main process variables such as pretreatment temperature, residence time, and solvent composition (aqueous ethanol) are studied. Results show that organosolv pretreatment causes delignification and hydrolysis of hemicelluloses and improves the enzymatic digestibility of olive tree pruning biomass. A higher pretreatment severity and ethanol content of the solvent were found to increase delignification (up to 64% at 66% w/w aqueous ethanol, 210°C, 60 min). By contrast, xylan hydrolysis was promoted by a lower ethanol content (maximum 92%). The highest enzymatic hydrolysis yield (90% of the structural glucan present in the raw material) has been obtained after pretreatment with 43% w/w aqueous ethanol at 210°C for 15 min. Organosolv pretreatment was found to be the most effective pretreatment for enzymatic hydrolysis of olive tree pruning biomass.

scholarly journals Endophytic Fungi as Pretreatment to Enhance Enzymatic Hydrolysis of Olive Tree Pruning

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Raquel Martín-Sampedro ◽  
Juan Carlos López-Linares ◽  
Úrsula Fillat ◽  
Guillermo Gea-Izquierdo ◽  
David Ibarra ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
Olive Tree ◽  
Wood Decomposition ◽  
Mild Acid Hydrolysis ◽  
Acid Pretreatment ◽  
Mediterranean Countries ◽  
Fungal Pretreatment ◽  
Hydrolysis Of ◽  
Tree Pruning ◽  
Mild Acid

Olive tree pruning, as one of the most abundant lignocellulosic residues in Mediterranean countries, has been evaluated as a source of sugars for fuel and chemicals production. A mild acid pretreatment has been combined with a fungal pretreatment using either two endophytes (Ulocladium sp. and Hormonema sp.) or a saprophyte (Trametes sp. I-62). The use of endophytes is based on the important role that some of them play during the initial stages of wood decomposition. Without acid treatment, fungal pretreatment with Ulocladium sp. provided a nonsignificant enhancement of 4.6% in glucose digestibility, compared to control. When a mild acid hydrolysis was carried out after fungal pretreatments, significant increases in glucose digestibility from 4.9% to 12.0% (compared to control without fungi) were observed for all fungal pretreatments, with maximum values yielded by Hormonema sp. However, despite the observed digestibility boost, the total sugar yields (taking into account solid yield) were not significantly increased by the pretreatments. Nevertheless, based on these preliminary improvements in digestibility, this work proves the potential of endophytic fungi to boost the production of sugar from olive tree pruning, which would add an extra value to the bioeconomy of olive crops.

scholarly journals Effect of Steam Explosion Pretreatment on Sugar Production by Enzymatic Hydrolysis of Olive Tree Pruning

2015 ◽  
Vol 81 ◽  
pp. 146-154 ◽  
Author(s):  
Marco Barbanera ◽  
Cinzia Buratti ◽  
Franco Cotana ◽  
Daniele Foschini ◽  
Elisa Lascaro
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Olive Tree ◽  
Sugar Production ◽  
Steam Explosion Pretreatment ◽  
Hydrolysis Of ◽  
Tree Pruning

scholarly journals Increase in Fermentable Sugars of Olive Tree Pruning Biomass for Bioethanol Production: Application of an Experimental Design for Optimization of Alkaline Pretreatment

2022 ◽  
Author(s):  
Arminda Mamaní ◽  
Yolanda Maturano ◽  
Laura Herrero ◽  
Laura Montoro ◽  
Fabiana Sardella
Keyword(s):  
Enzymatic Hydrolysis ◽  
Calcium Hydroxide ◽  
Sugar Content ◽  
Olive Tree ◽  
Operating Conditions ◽  
Alkaline Pretreatment ◽  
Fractional Factorial ◽  
Fermentable Sugars ◽  
Good Representation ◽  
Tree Pruning

Olive Tree Pruning (OTP) biomass can be considered a suitable source of fermentable sugars for the production of second-generation bioethanol. The present study proposes a remarkable alternative for the valorization of olive tree pruning residues. OTP biomass was processed using a sequential calcium hydroxide pretreatment/enzymatic hydrolysis. A 24–1 half fractional factorial design was adopted for the screening of process variables and a central composite design was used for the optimization stage. Temperature and lime loading resulted statistically significant. The following optimal conditions were obtained: 0.01 g of Ca(OH)2/g of dry material, 20 g of H2O/g of dry material at 160 °C for 2 h. The mathematical model that governs this alkaline pretreatment was obtained with a 76% adjusted determination coefficient, which means that it is a good representation of the process. Under optimal operating conditions, 13% of the cellulose and 88% of the hemicellulose was solubilized. Moreover, the fermentable sugar content increased 1800% compared with the initial conditions, obtaining 240 g of glucose per kg of OTP residue. The fermentable sugars obtained after the calcium hydroxide pretreatment and enzymatic hydrolysis of OTP biomass yielded 2.8 g of ethanol/100 g of raw material.

Enhanced enzymatic hydrolysis of olive tree wood by steam explosion and alkaline peroxide delignification

2006 ◽  
Vol 41 (2) ◽  
pp. 423-429 ◽  
Author(s):  
Cristóbal Cara ◽  
Encarnación Ruiz ◽  
Ignacio Ballesteros ◽  
María J. Negro ◽  
Eulogio Castro
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Olive Tree ◽  
Tree Wood ◽  
Alkaline Peroxide ◽  
Hydrolysis Of ◽  
Peroxide Delignification

Influence of solid loading on enzymatic hydrolysis of steam exploded or liquid hot water pretreated olive tree biomass

2007 ◽  
Vol 42 (6) ◽  
pp. 1003-1009 ◽  
Author(s):  
Cristóbal Cara ◽  
Manuel Moya ◽  
Ignacio Ballesteros ◽  
Ma José Negro ◽  
Alberto González ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Olive Tree ◽  
Hot Water ◽  
Solid Loading ◽  
Tree Biomass ◽  
Liquid Hot Water ◽  
Hydrolysis Of

scholarly journals Saccharification Yield through Enzymatic Hydrolysis of the Steam-Exploded Pinewood

Energies ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4552 ◽  
Author(s):  
Merve Nazli Borand ◽  
Asli Isler Kaya ◽  
Filiz Karaosmanoglu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Liquid Fraction ◽  
Sugar Content ◽  
Release Time ◽  
Variable Parameters ◽  
Glucose Yield ◽  
Steam Explosion Pretreatment ◽  
Saccharification Yield ◽  
Hydrolysis Of

Pressure, temperature, and retention time are the most studied parameters in steam explosion pretreatment. However, this work aimed to fix these parameters and to evaluate the influences of several less investigated steam explosion parameters on the saccharification yield in hydrolysis. In this study, firstly, pinewood samples smaller than 200 µm were treated with steam explosion at 190 °C for 10 min. The variable parameters were biomass loading, N2 pressure, and release time. Steam-exploded samples were hydrolyzed with the Trichoderma reesei enzyme for saccharification for 72 h. The sugar content of the resultant products was analyzed to estimate the yield of sugars (such as glucose, xylose, galactose, mannose, and arabinose). The best glucose yield in the pulp was achieved with 4 g of sample, N2 pressure of 0.44 MPa, and short release time (22 s). These conditions gave a glucose yield of 97.72% in the pulp, and the xylose, mannose, galactose, and arabinose yields in the liquid fraction were found to be 85.59%, 87.76%, 86.43%, and 90.3%, respectively.

Comparison of Enzymatic Hydrolysis of Leaves, Husks and Pith of Corn Stalk

2011 ◽  
Vol 365 ◽  
pp. 240-244 ◽  
Author(s):  
Yu Fei Dong ◽  
Jie Lu ◽  
Hui Jin ◽  
Rui Feng Yang ◽  
Teng Fei Yin
Keyword(s):  
Chemical Composition ◽  
Enzymatic Hydrolysis ◽  
Sugar Content ◽  
Morphological Structure ◽  
Morphological Characters ◽  
Corn Stalk ◽  
Enzyme Adsorption ◽  
Hydrolysis Of ◽  
Adsorption Capability

Corn stalk was divided into fractions by morphological characters, and the enzymatic hydrolysis of these fractions was investigated. Chemical composition and the proportions of cells of different compositions were very variable in different morphological structure fractions. This has a fundamental effect on the enzyme adsorption capability and hydrolysis performance. Cellulase, β-glucanase and xylanase were added to the husks, leaves, pith and whole stalks of cornstalks. The results of enzymatic hydrolysis of different morphological structure fractions of corn stalk were compared. It can be seen that the content of sugar degraded by β-glucanase is the highest. The fastest hydrolytic rate and the highest sugar content were obtained in the pith of corn stalks.

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