scholarly journals Effectiveness of salicylic acid, Pseudomonas fluorescens CHA0 and Trichoderma viride to control Meloidogyne incognita race 2 on different tomato cultivars

2016 ◽  
Vol 9 (1) ◽  
pp. 35-43 ◽  
Author(s):  
L. Esfahani ◽  
S. Jamali ◽  
A. Saeedizadeh ◽  
H. Pedramfar
Keyword(s):  
Salicylic Acid ◽  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Meloidogyne Incognita ◽  
Trichoderma Viride ◽  
Biocontrol Agents ◽  
Egg Mass ◽  
Root Weight ◽  
Tomato Cultivars ◽  
Race 2

Summary The effects of salicylic acid (SA), Trichoderma viride and Pseudomonas fluorescens CHA0 were studied on the root-knot nematode Meloidogyne incognita race 2 in resistant and susceptible tomato cultivars (Gina VF, Falat CH, Falat 111, Karoon) during 2012-2013. Four-leaf tomato seedlings were used, grown in pots containing 1000 g of sterilized soil; each seedling receiving 20 ml of T. viride suspension containing 1×106 spores, 30 ml of P. fluorescens CHA0 with 109 cfu/ml, 5mM of salicylic acid and 2000 second stage nematode juveniles. Parameters relevant to nematode population and plant growth were evaluated. The biocontrol agents and salicylic acid were effective in nematode control in combined and single treatments. High reductions in root galling and egg mass indices were observed with combination of SA and biocontrol agents. The greatest increase in plant growth was obtained when cv. Falat CH was treated with SA followed by P. fluorescens CHA0 and T. viride. The highest number of galls was recorded in cv. Karoon, followed by cvs. Falat 111, Gina VF and Falat CH. Pseudomonas fluorescens CHA0 provoked the highest increase in fresh and dry root weight, fresh and dry shoot weight and plant length in all free nematode treatments. The results indicated that chemical inducer (salicylic acid), in combination with biocontrol agents (T. viride and P. fluorescens CHA0), stimulated and eventually increased plant growth.

scholarly journals Management of Root Knot Nematode Meloidogyne incognita in Banana (Cv. Robusta) through Biocontrol Agents and Neem Cake

2020 ◽  
Author(s):  
P. Saravanan ◽  
N. Ilavarasan ◽  
A. Karthikeyan ◽  
B. Padmanaban
Keyword(s):  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Growth Parameters ◽  
Trichoderma Viride ◽  
Control Plant ◽  
Biocontrol Agents ◽  
Root Knot Nematode ◽  
Banana Plant ◽  
Neem Cake ◽  
Gm Plant

A pot culture experiment was performed to study the effect of native promising biocontrol agents and neem cake separately and in combination against root-knot nematode Meloidogyne incognita infecting banana cv. Robusta during 2016-2017. Treatments treated with bioagents and neem cake enhanced plant growth and root characters and suppressed root gall development in banana plant as compared to nematode alone and untreated control treatments. Among the various treatments, the combined application of Pseudomonas fluorescens @ 10 g/plant + Trichoderma viride @ 10 gm/plant + neem cake @ 100 gm/plant resulted in maximum increase of plant height (38.8 cm), number of leaves (7), pseudostem girth (15 cm), root length (25.6 cm), highest number of healthy roots (17.0), poor number of infected roots (3.3), lowest root gall index (1 scale) and significantly reduced root-knot nematode population both soil (55.0 nos) and roots (90.0 nos). The application of single bioagent either P.fluorescens @ 10 gm/plant or T.viride @ 10 gm/plant along with neem cake were also recorded highest plant growth parameters and poor nematode infestation when compared to nematode alone treated control plant.

scholarly journals Effect of oil cakes and garlic aqueousbased formulations of trichoderma viride on management of meloidogyne incognita in chilli

2018 ◽  
Vol 3 (1) ◽  
pp. 214-219
Author(s):  
A. Nirosha ◽  
S. Rajeshkanna ◽  
G. Mikunthan
Keyword(s):  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Trichoderma Viride ◽  
Neem Oil ◽  
Root Knot Nematode ◽  
Chemical Application ◽  
Spore Yield ◽  
Mahua Oil ◽  
Madhuca Longifolia ◽  
Oil Cakes

Abstract Root-knot nematode, Meloidogyne incognita (Kofoid and White) is a major threat to chilli (Capsicum annum) cultivation, by forming root galls and subsequent wilting. These nematodes live in soil, roots debris and reported surviving in other Solanaceae crops. Chemical application of nematicides cause impact on environment, therefore biocontrol using antagonistic fungi is desired to tackle this problem. This research is aimed to evaluate the effect of Trichoderma viride and its formulations on management of M. incognita. Oil cakes such as neem (Azadiracta indica), gingelly (Sesamum indicum), Mahua (Madhuca longifolia) and garlic (Allium sativum) extracts were used to evaluate the growth and sporulation of T. viride. Chilli variety MI-2 was used. Among the four preparations, neem oil cake recorded high spore yield of 1.75 ×107 spores/ml. Similarly gingelly oil cake and garlic produced the spore yields of 1.57 ×107 spores/ml and 1.368 ×107 spores/ml, respectively. Comparatively Mahua oil cake obtained low spore yield of 7.87×106 spores/ml. Plant growth was significant (P<0.05) in the application of neem oil cake formulation (30.42 cm). Extent of galling was significantly low with garlic (9.00) and neem (9.25). These results confirmed that the formulations of neem, gingelly oil cake extracts and garlic aqueous extract stimulated the chilli plant growth, productivity and reduced the nematode infestation. Above all, neem oil cake and garlic are the best formulations that can be used to manage M. incognita

scholarly journals Eco-friendly based Integrated Management Practice against Root-Knot Nematode, Meloidogyne incognita on Cluster bean (Cyamopsis tetragonoloba L.)

2021 ◽  
Author(s):  
Chandra Prakash Nama ◽  
B.L. Baheti
Keyword(s):  
Ascorbic Acid ◽  
Salicylic Acid ◽  
Meloidogyne Incognita ◽  
Management Practice ◽  
Nematode Population ◽  
Egg Mass ◽  
Cyamopsis Tetragonoloba ◽  
Root Knot Nematode ◽  
Cluster Bean ◽  
Neem Leaves

Abstract AimThe experiment was carried out during two consecutive Kharif seasons to test the combined efficacy of biochemicals i.e. salicylic acid, ascorbic acid and L-arginine each at 2 % w/w as seed treatment and botanicals viz. neem, lantana and parthenium leaves powder each at 5 g per plant for the management of root-knot nematode, Meloidogyne incognita infecting cluster bean..Method All treatments applied as soil application at the time of sowing. Before sowing observation on initial nematode population/100 cc soil and at the termination of experiment number of galls/plant, number of egg masses/plant, number of eggs and larvae/egg mass, final nematode population/100 cc soil and yield (q/ha) were recorded. ResultAmong different combinations, minimum nematode population were observed with ascorbic acid at 2 per cent w/w + neem leaves powder at 5 g per plant followed by ascorbic acid at 2 per cent + lantana leaves powder at 5 g per plant and salicylic acid at 2 per cent + neem leaves powder at 5 g per plant.Conclusion: Results of experiment showed that application of biochemicals along with botanicals significantly reduced galls per plant as compared to untreated check.

Effect of Some Bacterial and Fungal Bioagents for Management of Root-knot Nematode and Soil Born Fungus in Cucumber under Protected Conditions

2021 ◽  
Author(s):  
Jaydeep Ashok Patil ◽  
Saroj Yadav ◽  
Sewak Ram
Keyword(s):  
Plant Growth ◽  
Complex Disease ◽  
Trichoderma Viride ◽  
Growth Parameter ◽  
Shoot Length ◽  
Root Weight ◽  
Liquid Formulation ◽  
Root Knot Nematode ◽  
Untreated Check ◽  
Shoot Weight

Abstract Background: Complex disease caused by root-knot nematode and Fusarium fungus in cucumber is the most destructive disease under polyhouse conditions. The study was conducted to determine the potential of biocontrol agents viz. Trichoderma viride, Pseudomonas fluorescence, Purpureocillium lilacinum against root-knot nematode, Meloidogyne incognita and Fusarium oxysporum f. sp. cucumerinum disease complex on cucumber.Results: All bioagents were taken @ 0.3 and 0.5 g/kg soil and liquid formulation of bioagents, @ 0.5 and 1 ml/ kg soil, Chemical checks as well as untreated check were also maintained. The bio-agents were mixed with the potted soil treatment wise. All the treatments significantly improved the plant growth parameter, viz., shoot length, root length, fresh shoot weight, fresh root weight, dry shoot weight and dry root weight as compared to untreated check. However, maximum improvement in plant growth parameter was recorded in case of carbofuran followed by higher dose of liquid formulation of bioagents. Among bioagents, liquid formulation of bioagents was significantly improved the plant growth parameter, viz., shoot length (147.3), fresh shoot weight (55.6), dry shoot weight (22.51) and dry root weight (4.50) and most effective in suppressing root knot nematode galling (43) and final population in soil (131) and fungus wilt incidence (25 %) at 30th day of after germination followed by P. lilacinum T. viride and P. fluorescence. Conclusion: The results suggest that the liquid formulation of bioagents was more effective in suppression of root-knot nematode and fungus complex disease than the powder formulations of bioagents. More studies should be needed in future to evaluate the efficacy of bioagents as seed treatments and soil applications under field conditions.

Evaluation of the effects of composted broiler litter enriched with Trichoderma viride in management of Meloidogyne incognita (Kofoid and White) in spinach (Spinacia oleracea)

2012 ◽  
Vol 2 (3) ◽  
pp. 90-96
Author(s):  
Lalithanjalie D. Amarasinghe ◽  
Thilini N. Madurusinghe
Keyword(s):  
Plant Growth ◽  
Meloidogyne Incognita ◽  
Spinacia Oleracea ◽  
Trichoderma Viride ◽  
Nematophagous Fungus ◽  
Broiler Litter ◽  
Naturally Occurring ◽  
Native Soil ◽  
Soil Microfauna ◽  
Recommended Level

In this study, repeat screen‐house experiments were carried out to evaluate the effects of composted broiler litter enriched with Trichoderma viride in management of Meloidogyne incognita (Kofoid and White) in spinach(Spinacia oleracea). A significant increase of plant growth in S. oleracea and adecrease in the population of M. incognita were observed in the amendedsoil (soil:composted broiler litter 22:1 v/v). Infested plants that were grownin native soil showed a significant reduction in growth, whereas in soilstreated with nematicide, carbofuran 3% G the growth was moderate. Theabundance of soil microfauna and naturally occurring nematophagous fungus,T. viride significantly increased in amended soil when compared to thesoil treated with chemical nematicides. This study concludes that compostedbroiler litter (soil:composted broiler litter 22:1 v/v) enriched with T. virideincreased the plant growth and abundance of soil microfauna and reducedthe population of M. incognita. Therefore, treatment with T. viride might bemore effective than the use of recommended level of chemical nematicides.

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