The consequences of deglycosylation of recombinant intra-melanosomal domain of human tyrosinase
Keyword(s):
AbstractTyrosinase, a melanosomal glycoenzyme, catalyzes initial steps of the melanin biosynthesis. While glycosylation was previously studiedin vivo, we present three recombinant mutant variants of human tyrosinase, which were obtained using multiple site-directed mutagenesis, expressed in insect larvae, purified and characterized biochemically. The mutagenesis demonstrated the reduced protein expression and enzymatic activity due to possible loss of protein stability and protein degradation. However, the complete deglycosylation of asparagine residuesin vitro, including the residue in position 371, interrupts tyrosinase function, which is consistent with a melanin loss in oculocutaneous albinism type 1 (OCA1) patients.
2001 ◽
Vol 183
(1)
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pp. 250-256
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2003 ◽
Vol 185
(20)
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pp. 6205-6208
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2013 ◽
Vol 57
(3)
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pp. 1379-1384
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Keyword(s):
1998 ◽
Vol 66
(8)
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pp. 3626-3634
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