Investigation of Consecutive Fouling and Cleaning Cycles of Ultrafiltration Membranes Used for Whey Processing

2014 ◽  
Vol 10 (3) ◽  
pp. 367-381 ◽  
Author(s):  
Thilo H. A. Berg ◽  
Jes C. Knudsen ◽  
Richard Ipsen ◽  
Frans van den Berg ◽  
Hans H. Holst ◽  
...  
Keyword(s):  
Protein Concentration ◽  
Ultrafiltration Membranes ◽  
Filtration Performance ◽  
Development Of Resistance ◽  
High Protein Concentration ◽  
Resistance Reduction ◽  
Layer Resistance ◽  
Cleaning Procedures ◽  
Feed Protein ◽  
Industrial Cleaning

Abstract Development of resistance during multiple foulings and three-step Cleaning-In-Place (CIP) operations, simulating an industrial cleaning regime of polysulfone ultrafiltration membranes, was investigated. The study explored how trans-membrane pressure (150 and 300 kPa) and feed protein concentration (0.9 and 10%) influenced resistance reduction during filtration and flux recovery by the cleaning procedures. New membranes, pre-cleaned with a full CIP cycle, were used for each experiment. Subsequent fouling (simulating production) and CIP were done three times in a row and the development of fouling layer resistance was monitored and evaluated. Results show that filtration performance decreased during the first days of usage, possibly related to build-up of internal fouling. Cleaning success based on flux recovery was negatively influenced by a high protein concentration in the feed, but independent of the trans-membrane pressure during filtration.

scholarly journals The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Olga Ilinskaya ◽  
Vera Ulyanova ◽  
Irina Lisevich ◽  
Elena Dudkina ◽  
Nataliya Zakharchenko ◽  
...  
Keyword(s):  
Conformational Changes ◽  
Protein Concentration ◽  
Bacillus Pumilus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Culture Fluid ◽  
Size Exclusion ◽  
High Protein Concentration ◽  
Exclusion Chromatography ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Hypochromic Effect

Supported by crystallography studies, secreted ribonuclease of Bacillus pumilus (binase) has long been considered to be monomeric in form. Recent evidence obtained using native polyacrylamide gel electrophoresis and size-exclusion chromatography suggests that binase is in fact dimeric. To eliminate ambiguity and contradictions in the data we have measured conformational changes, hypochromic effect, and hydrodynamic radius of binase. The immutability of binase secondary structure upon transition from low to high protein concentration was registered, suggesting the binase dimerization immediately after translocation through the cell membrane and leading to detection of binase dimers only in the culture fluid regardless of ribonuclease concentration. Our results made it necessary to take a fresh look at the binase stability and cytotoxicity towards virus-infected or tumor cells.

Challenges in the development of high protein concentration formulations

2004 ◽  
Vol 93 (6) ◽  
pp. 1390-1402 ◽  
Author(s):  
Steven J. Shire ◽  
Zahra Shahrokh ◽  
Jun Liu
Keyword(s):  
Protein Concentration ◽  
High Protein ◽  
High Protein Concentration

scholarly journals Filtration performance and pore size distribution of hypochlorite aged PES/PVP ultrafiltration membranes

2015 ◽  
Vol 474 ◽  
pp. 175-186 ◽  
Author(s):  
Bastien Pellegrin ◽  
Fernanda Mezzari ◽  
Yamina Hanafi ◽  
Anthony Szymczyk ◽  
Jean-Christophe Remigy ◽  
...  
Keyword(s):  
Pore Size Distribution ◽  
Pore Size ◽  
Size Distribution ◽  
Ultrafiltration Membranes ◽  
Filtration Performance

AAC Richard Soybean

2022 ◽  
Author(s):  
Kangfu Yu ◽  
Lorna Woodrow ◽  
M. Chun Shi
Keyword(s):  
Glycine Max ◽  
Research And Development ◽  
Soybean Cyst Nematode ◽  
Protein Concentration ◽  
Heterodera Glycines ◽  
Cyst Nematode ◽  
Processing Quality ◽  
Food Grade ◽  
High Protein Concentration ◽  
Glycine Max L

AAC Richard is a food grade soybean [Glycine max (L.) Merr] cultivar with yellow hilum, high protein concentration, and good processing quality for foreign and domestic soymilk, tofu, and miso markets. It has resistance to SCN (soybean cyst nematode) (Heterodera Glycines Ichinohe). AAC Richard was developed at the Agriculture and Agri-Food Canada (AAFC) Harrow Research and Development Centre (Harrow-RDC), Harrow, Ontario and is adapted to areas of southwest Ontario with 3100 or more crop heat units and has a relative maturity of 2.3 (MG 2.3).

Recovery from increased pressure or increased leakiness edema in perfused sheep lungs

1994 ◽  
Vol 77 (1) ◽  
pp. 184-189 ◽  
Author(s):  
M. Fukue ◽  
V. B. Serikov ◽  
E. H. Jerome
Keyword(s):  
Pulmonary Edema ◽  
Osmotic Pressure ◽  
Pulmonary Circulation ◽  
Protein Concentration ◽  
Lymph Flow ◽  
High Protein ◽  
Osmotic Gradient ◽  
Low Protein ◽  
High Protein Concentration ◽  
Interstitial Pulmonary Edema

Two routes by which interstitial pulmonary edema liquid may leave the lung during recovery are reabsorption into the pulmonary circulation and clearance by lung lymphatics. We hypothesized that reabsorption of edema liquid of low protein concentration into the pulmonary circulation would be greater than reabsorption of edema liquid of high protein concentration because of the greater protein osmotic gradient in the former. On the basis of previous studies, lymph flow should contribute minimally to the recovery. In 22 in situ perfused sheep lungs with lymph fistulas, we produced approximately 100 g of osmotic or hydrostatic edema (low protein) or increased leakiness edema by calcium depletion (high protein). To induce reabsorption, we changed the perfusate from low- (1% albumin, osmotic pressure = 4 cmH2O) to high-protein (7% albumin, osmotic pressure = 22 cmH2O) solution in the osmotic group, decreased capillary pressure from 29 +/- 9 to 11 +/- 6 cmH2O in the hydrostatic group, or reversed leakiness by adding CaCl2 to the perfusate in the increased leakiness group. Reabsorption occurred only during recovery from osmotic (40 +/- 22% of filtered liquid) and hydrostatic (15 +/- 11%) edema. Total lung lymph flow during recovery from osmotic, hydrostatic, or increased leakiness edema was 4.9 +/- 3.4, 4.3 +/- 3.4, or 3.5 +/- 1.9 g, respectively. We conclude that during recovery from pulmonary edema interstitial liquid is reabsorbed into the circulation in inverse proportion to its protein concentration. We confirm that only a small fraction of the interstitial edema liquid is cleared by the lymphatics during recovery from any type of edema.

Registration of HS-182 and HS-183 food-grade soybean [Glycine max (L.) Merr.] germplasm

2019 ◽  
Vol 99 (4) ◽  
pp. 568-571
Author(s):  
K. Yu ◽  
L. Woodrow ◽  
M. Chun Shi ◽  
D. Anderson
Keyword(s):  
Glycine Max ◽  
Food Processing ◽  
Protein Concentration ◽  
Seed Protein ◽  
Protein Profiles ◽  
Processing Quality ◽  
Food Grade ◽  
High Protein Concentration ◽  
Glycine Max L ◽  
Maturity Groups

HS-182 and HS-183 are food-grade soybean lines [Glycine max (L.) Merr.] with distinct seed protein profiles and food processing quality. HS-182 is a 7S β-conglycinin α’ and 11S glycinin A4 null with a high protein concentration of 45.7% and good processing quality. HS-183 is a 7S β-conglycinin α’ and 11S glycinin null with a protein concentration of 42.7% and poor tofu processing quality. They are adapted to areas of southwestern Ontario with 3100 or more crop heat units and have relative maturity groups of 2.5 and 2.4, respectively.

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