Symmetry Element

2016 ◽  
Author(s):  
Vladimir I. Minkin
Keyword(s):  
1987 ◽  
Vol 7 (3) ◽  
pp. 1217-1225
Author(s):  
M E Greenberg ◽  
Z Siegfried ◽  
E B Ziff

In vitro mutagenesis of a 61-base-pair DNA sequence element that is necessary for induction of the c-fos proto-oncogene by growth factors revealed that a small region of dyad symmetry within the sequence element is critical for c-fos transcriptional activation. The same c-fos dyad symmetry element was found to bind a nuclear protein in vitro, causing a specific mobility shift of this c-fos regulatory sequence. An analysis of insertion and deletion mutants established a strict correlation between the ability of the dyad symmetry element to promote serum activation of c-fos transcription and in vitro nuclear protein binding. These experiments suggest that the DNA mobility shift assay detects a nuclear protein that mediates growth factor stimulation of c-fos expression. In vitro competition experiments indicate that the c-fos regulatory factor also binds to sequences within another growth factor-inducible gene, the beta-actin gene.


Blood ◽  
1991 ◽  
Vol 77 (1) ◽  
pp. 55-63 ◽  
Author(s):  
D Trouche ◽  
P Robin ◽  
P Sassone-Corsi ◽  
WL Farrar ◽  
A Harel-Bellan

Abstract The c-fos proto-oncogene seems to play an important role during differentiation and activation of cells from the hematopoietic lineage. Therefore, it is of interest to investigate the mechanism underlying its transcriptional activation in these cells. To delineate the sequences and factors involved in c-fos transcriptional activation during the course of myeloid cell differentiation, we have used the K 562 chronic leukemic cell line as a model. K 562 cells were transfected with chloramphenicol transacetylase (CAT) reporter constructs, including various regions of the human c-fos promoter, and induced to differentiate by two distinct agents: 12-O-tetradecanoyl phorbol-13- acetate (TPA), which activates a differentiation program along the megakaryoblastic pathway; and hemin, which induces erythroid differentiation. We show here that TPA treatment of K 562 cells induces fos CAT reporter constructs activation, whereas treatment with hemin does not. Furthermore, predifferentiation of the cells with hemin blocks a subsequent induction by TPA, in correlation with the inhibition by hemin of megakaryoblastic differentiation markers appearance. Both the induction by TPA and the inhibition by hemin are mediated by a dyad symmetry element (DSE) located in the upstream regulatory region, between -318 and -296. These results suggest that the protein complex binding to the DSE regulatory element is the target for c-fos activation by TPA and inhibition by hemin in K 562 cells. However, no modulation of protein affinity for the DSE sequence was detected by gel shift assay during the course of induction or inhibition, suggesting that the structural change responsible for the transcriptional modulation is too unstable or too subtle to be detected by this method.


2012 ◽  
Vol 68 (4) ◽  
pp. o1037-o1038 ◽  
Author(s):  
A. S. Dayananda ◽  
Ray J. Butcher ◽  
Mehmet Akkurt ◽  
H. S. Yathirajan ◽  
B. Narayana

In the triprolidinium cation of the title compound {systematic name: 2-[1-(4-methylphenyl)-3-(pyrrolidin-1-ium-1-yl)prop-1-en-1-yl]pyridin-1-ium bis(2,5-dichloro-4-hydroxy-3,6-dioxocyclohexa-1,4-dien-1-olate)–2,5-dichloro-3,6-dihydroxycyclohexa-2,5-diene-1,4-dione–methanol–water (2/1/2/2)}, C19H24N22+·2C6HCl2O4−·0.5C6H2Cl2O4·CH3OH·H2O, the N atoms on both the pyrrolidine and pyridine groups are protonated. The neutral chloranilic acid molecule is on an inversion symmetry element and its hydroxy H atoms are disordered over two positions with site-occupancy factors of 0.53 (6) and 0.47 (6). The methanol solvent molecule is disordered over two positions in a 0.836 (4):0.164 (4) ratio. In the crystal, N—H...O, O—H...O and C—H...O interactions link the components. The crystal structure also features π–π interactions between the benzene rings [centroid–centroid distances = 3.5674 (15), 3.5225 (15) and 3.6347 (15) Å].


1980 ◽  
Vol 20 (04) ◽  
pp. 293-303 ◽  
Author(s):  
Jacques Hagoort ◽  
Brian D. Weatherill ◽  
Antonin Settari

Abstract A mathematical reservoir model is presented to simulate the propagation of waterflood-induced hydraulic fractures in a symmetry element of a waterflood pattern. The model consists of a conventional single-phase reservoir simulator coupled with an analytical fracture model.The model is capable of simulating fracture propagation as a function of (1) injection and propagation as a function of (1) injection and production rates or pressures, (2) reservoir and fluid production rates or pressures, (2) reservoir and fluid properties, and (3) formation-fracturing pressures. properties, and (3) formation-fracturing pressures. Examples are given that clearly illustrate the characteristics of hydraulic-fracture growth. The key variables are injection rate and voidage/replacement ratio. Fractures can be contained by restricting the injection rate and by imposing a voidage/ replacement ratio equal to or less than 1.The modeling technique presented here also may be applied to other fracturing problems, particularly in cases where leakoff is significant and, thus, should be taken into account rigorously. Introduction Injection pressures in water-injection wells often exceed the formation-fracturing pressures, either unintentionally or by design. Although this leads to improved injectivity, it might jeopardize the flooding efficiency of waterfloods; induced hydraulic fractures can grow to be very long, thereby adversly affecting areal sweep efficiency.At present, quantitative information on the propagation of waterflood-induced hydraulic propagation of waterflood-induced hydraulic fractures is scarce. Theories of hydraulic fracturing do exist but are geared to controlled fracture-stimulation techniques. In these techniques the induced fractures and propagation times are relatively short and fluid losses are kept low by using additives in the fracture fluids. Waterflood-induced fractures, on the other hand, may grow for many years and are characterized by high fluid losses.In this paper a mathematical model for simulating hydraulic fracture growth under waterflood conditions is presented. The model is capable of calculating fracture propagation in a two-dimensional symmetry element of a unit mobility ratio waterflood containing one injector and one or more producers. With this model, fracture growth can be investigated as a function of (1) injection rates and production rates or pressures, (2) reservoir and fluid properties, and (3) formation-fracturing pressures. pressures. Several examples are given that clearly illustrate the general growth characteristics of waterflood-induced hydraulic fractures.The model presented is relatively simple in scope and is certainly open to improvements. Yet in its present form it is already a valuable diagnostic and present form it is already a valuable diagnostic and predictive tool that can be used to manage fractured predictive tool that can be used to manage fractured waterfloods better. Physical Model Physical Model Fig. 1 depicts the physical model we wish to simulate. It consists of (1) a symmetry element of a waterflood reservoir containing injection and production wells, and (2) a fracture extending from an injection well in a chosen direction.In this model the reservoir has a uniform or slightly varying thickness that is small relative to the areal dimensions. The mobility ratio of the waterflood is M = 1.0. In this system fluid flow in the reservoir can be approximated by the equation for two-dimensional single-phase compressible flow. SPEJ P. 293


1998 ◽  
Vol 54 (5) ◽  
pp. 507-514 ◽  
Author(s):  
V. Schomaker ◽  
K. N. Trueblood

The simple one-parameter Dunitz–White model for internal torsional motion accompanying overall molecular motion in crystals ignores the correlations between this torsion and the overall translation and libration. These correlations are explicitly considered here, in quadratic approximation (based on the linear approximation for displacements). For each attached rigid group (ARG) undergoing a torsional libration, there are, in addition to the mean-square libration amplitude, six correlations to be considered, three with the overall molecular libration and three with the overall translation. Because it is impossible from the observed quadratic mean displacements to distinguish the torsional motion from the overall molecular libration parallel to the torsional axis, the present analysis emphasizes the overall libration parallel to the torsional axis, which we term \Lambda. In the general case there are only six determinable parameters for each ARG. If the torsional axis of the ARG lies on a molecular symmetry element, the number of parameters is reduced to three or fewer. Examples of analyses with these correlations included, and without them, are compared.


Blood ◽  
1991 ◽  
Vol 77 (1) ◽  
pp. 55-63
Author(s):  
D Trouche ◽  
P Robin ◽  
P Sassone-Corsi ◽  
WL Farrar ◽  
A Harel-Bellan

The c-fos proto-oncogene seems to play an important role during differentiation and activation of cells from the hematopoietic lineage. Therefore, it is of interest to investigate the mechanism underlying its transcriptional activation in these cells. To delineate the sequences and factors involved in c-fos transcriptional activation during the course of myeloid cell differentiation, we have used the K 562 chronic leukemic cell line as a model. K 562 cells were transfected with chloramphenicol transacetylase (CAT) reporter constructs, including various regions of the human c-fos promoter, and induced to differentiate by two distinct agents: 12-O-tetradecanoyl phorbol-13- acetate (TPA), which activates a differentiation program along the megakaryoblastic pathway; and hemin, which induces erythroid differentiation. We show here that TPA treatment of K 562 cells induces fos CAT reporter constructs activation, whereas treatment with hemin does not. Furthermore, predifferentiation of the cells with hemin blocks a subsequent induction by TPA, in correlation with the inhibition by hemin of megakaryoblastic differentiation markers appearance. Both the induction by TPA and the inhibition by hemin are mediated by a dyad symmetry element (DSE) located in the upstream regulatory region, between -318 and -296. These results suggest that the protein complex binding to the DSE regulatory element is the target for c-fos activation by TPA and inhibition by hemin in K 562 cells. However, no modulation of protein affinity for the DSE sequence was detected by gel shift assay during the course of induction or inhibition, suggesting that the structural change responsible for the transcriptional modulation is too unstable or too subtle to be detected by this method.


1982 ◽  
Vol 46 (339) ◽  
pp. 265-268 ◽  
Author(s):  
W. A. Wooster

AbstractBy constructing ball-and-spoke models an attempt has been made to arrive at the atomic arrangements on the twin boundaries of four twins of calcite and one of aragonite. The Miller indices of the twins of calcite are (111), (110), (100) and (11). The twin plane in aragonite is (110). Each of the models of calcite twins has the same plane for twin plane and composition plane, and in each case it is a true mirror plane. Aragonite has a pseudo glide plane parallel to (110) gliding in the [001] direction. By making this pseudo symmetry element into a true glide plane a good model of the twin is obtained. In the (111), (110) and (100) twins of calcite all the CO3 groups in the composition plane are parallel to it. In the (11) twin the composition plane contains only CO3 groups and these are perpendicular to it.


1989 ◽  
Vol 8 (9) ◽  
pp. 2559-2566 ◽  
Author(s):  
H. König ◽  
H. Ponta ◽  
U. Rahmsdorf ◽  
M. Büscher ◽  
A. Schönthal ◽  
...  

Tetrahedron ◽  
2021 ◽  
pp. 132340
Author(s):  
Haoran Xue ◽  
Haleigh Svatek ◽  
Ariane F. Bertonha ◽  
Keighley Reisenauer ◽  
Joshua Robinson ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document