24-Epibrassinolide Restores the Synthesis of Proteins and Amino Acids in Brassica juncea L. Leaves Under Imidacloprid Stress

Abstract Pesticides are applied to protect crops from a variety of insect pests but their application cause toxicity to plants that results, among others, in reduction of protein as well as amino acid contents. The present study is aimed at observing the effect of seed pre-soaking with 24-epibrassinolide (EBL) on the protein and amino acid content in the leaves of Brassica juncea L. grown in soil that is amended with pesticide im-idacloprid (IMI). Soil amendment with IMI resulted in a decrease in the contents in leaves of total proteins and 21 amino acids studied. Seed soaking with 100 nM of EBL resulted in the recovery of total protein as well as amino acid contents in leaves, when compared with plants grown in only IMI amended soils.

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AMINO ACID AND PROTEIN CONTENT OF Poa pratensis AS RELATED TO NITROGEN APPLICATION AND COLOR

Canadian Journal of Plant Science ◽

10.4141/cjps84-095 ◽

1984 ◽

Vol 64 (3) ◽

pp. 691-697 ◽

Cited By ~ 3

Author(s):

S. H. NELSON ◽

F. W. SOSULSKI

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Total Protein ◽

Acid Content ◽

Soluble Protein ◽

Poa Pratensis ◽

Amino Acid Content ◽

Kentucky Bluegrass ◽

Proline Content ◽

Nitrogen Application

Kentucky bluegrass (Poa pratensis L.) plots receiving 2.5, 2.0, 1.7, 1.2, or 0.8 kg N∙100 m−2 each season in three equal amounts on approximately 15 May, 1 July, and 15 Aug. were analyzed for protein and amino acid content, as well as for the proportions of alcohol-soluble and salt-soluble proteins. Aspartic acid content of protein was the only amino acid that showed a positive regression with both the amount of nitrogen applied and turfgrass color, while the proline content of the dormant grass was highly dependent upon the amount of nitrogen applied during the summer. Total protein was highly dependent upon the nitrogen applied throughout the season and may, in part, explain the greener spring and fall color but, more probably, the high levels of salt-soluble protein in spring and fall mediated the spring and fall greening effects observed. At least 1.7 kg N∙100 m−2 season was required to ensure early greening the following spring.Key words: Kentucky bluegrass, Poa pratensis, nitrogen, amino acids, protein fractions, color

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Relation of wheat species and genomes in amino-acid composition

Review on Agriculture and Rural Development ◽

10.14232/rard.2018.1-2.16-22 ◽

2019 ◽

Vol 7 (1-2) ◽

pp. 16-22

Author(s):

Pal Pepo

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Winter Wheat ◽

Acid Content ◽

Essential Amino Acid ◽

Amino Acid Content ◽

Total Amino Acid ◽

Wheat Varieties ◽

Amino Acid Contents ◽

Winter Wheat Varieties

As regards wheat varieties constituting a natural ploid series the issue of analysing diploid, tetraploid and hexaploid species is topical since ancient varieties can play significant roles in contemporary agriculture as well. Seventeen winter wheat varieties, out of which 2 diploid varieties carried genome A, 9 diploidic types had genomes AB, two varieties had genomes AG and four varieties were hexaploid ones with genomes ABD, were analysed from the point of view of their amino acid compositions. The amino acid contents of Asp, Thr, Ser, Glu, Gly, Ala, Cys, Val, Met, Ile, Leu, Tyr, Phe, His, Lys, Arg, Pro (a total of 17) were determined in the varieties listed above. It has been found that the amino acid contents of the grains genotype AA Triticum boeticum and T. monococcum exceeded the amino acid content of T. aestivum in respect of all the amino acids analysed in this experiment, with Glu being the only exception. In comparison with the aestivum wheat, essential amino acid contents showed a similarly favourable picture in the diploidic varieties mentioned. As regards type AB tetraploid varieties excesses of 13-16%, in comparison to the aestivum wheat, were found in essential amino acid contents. The amounts of non-essential amino acids in all the winter wheat varieties showed decreases irrespective of the ploid level. What concerns the total amino acid content, all the winter wheat varieties with the exception of T. monococcum (A), T. dicoccoides (AB) and T. dicoccum (AB) contained less amino acid than the aestivum wheat. All the monocarbonic acid and aromatic as well as heterocyclic amino acid contents of the wildly growing Triticum boeticum (A) and the grown Triticum monococcum (A) (with polaric, apolaric R groups, diamino radicles) exceeded the same contents of T. aestivum. The value of the monoamino-dicarbonic acid, however, was lower in our experiment.

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Amino acids through developmental stages of sunflower leaves

Acta Botanica Croatica ◽

10.2478/v10184-012-0009-5 ◽

2013 ◽

Vol 72 (1) ◽

pp. 23-33 ◽

Cited By ~ 3

Author(s):

Nayan Roy ◽

Subrata Laskar ◽

Anandamay Barik

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Total Protein ◽

Acid Content ◽

Free Amino Acids ◽

Free Amino ◽

Developmental Stages ◽

Amino Acid Content ◽

Free Amino Acid Content ◽

Protein Levels

Abstract The PICO-TAGanalysis of proteins revealed that 17 protein-bound and 18 free amino acids were present throughout the developmental stages of sunflower leaves. The total protein-bound amino acid content was much higher than total free amino acid content throughout the development of sunflower leaves. The contents of protein-bound and free amino acids as well as essential and non-essential ones displayed different patterns with leaf maturation, suggesting that total protein levels are poor predictors of the nutritive status of leaves.

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Evaluation of seed amino acid content and its correlation network analysis in wild soybean (Glycine soja) germplasm in Japan

Plant Genetic Resources ◽

10.1017/s1479262121000071 ◽

2021 ◽

Vol 19 (1) ◽

pp. 35-43

Author(s):

Awatsaya Chotekajorn ◽

Takuyu Hashiguchi ◽

Masatsugu Hashiguchi ◽

Hidenori Tanaka ◽

Ryo Akashi

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Free Amino Acid ◽

Acid Content ◽

Free Amino ◽

Glycine Soja ◽

Wild Soybean ◽

Amino Acid Content ◽

Soybean Seeds ◽

Free Amino Acid Content

AbstractWild soybean (Glycine soja) is a valuable genetic resource for soybean improvement. Seed composition profiles provide beneficial information for the effective conservation and utilization of wild soybeans. Therefore, this study aimed to assess the variation in free amino acid abundance in the seeds of wild soybean germplasm collected in Japan. The free amino acid content in the seeds from 316 accessions of wild soybean ranged from 0.965 to 5.987 mg/g seed dry weight (DW), representing a 6.2-fold difference. Three amino acids had the highest coefficient of variation (CV): asparagine (1.15), histidine (0.95) and glutamine (0.94). Arginine (0.775 mg/g DW) was the predominant amino acid in wild soybean seeds, whereas the least abundant seed amino acid was glutamine (0.008 mg/g DW). A correlation network revealed significant positive relationships among most amino acids. Wild soybean seeds from different regions of origin had significantly different levels of several amino acids. In addition, a significant correlation between latitude and longitude of the collection sites and the total free amino acid content of seeds was observed. Our study reports diverse phenotypic data on the free amino acid content in seeds of wild soybean resources collected from throughout Japan. This information will be useful in conservation programmes for Japanese wild soybean and for the selection of accessions with favourable characteristics in future legume crop improvement efforts.

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The Oocytes of the Goby Pomatoschistus minutus. III. Determination of Amino Acid Component

Zeitschrift für Naturforschung C ◽

10.1515/znc-1980-11-1239 ◽

1980 ◽

Vol 35 (11-12) ◽

pp. 1094-1095

Author(s):

Rüdiger Riehl

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Glutamic Acid ◽

Acid Content ◽

Amino Acid Content ◽

Acid Component ◽

Pomatoschistus Minutus ◽

Protein Amino Acids ◽

Amino Acid Component

Abstract The oocytes of the marine goby Pomatoschistus minutus were analyzed for their amino acid content. Most of the amino acids exist as protein, only a little part is free or peptide-bound. Among the protein-bound amino acids, high levels of glutamic acid, proline, alanine, aspartic acid, valine and leucine were detected. These represent more than 60% of the protein amino acids. Among the free acids, glutamic acid, serine and alanine, are dominant. There are no certain proofs of the occurrence of peptide pools in the oocytes of Pomatoschistus minutus.

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THE AMINO ACID COMPOSITION OF HYPERTENSIN II AND ITS BIOCHEMICAL RELATIONSHIP TO HYPERTENSIN I

Journal of Experimental Medicine ◽

10.1084/jem.104.2.183 ◽

1956 ◽

Vol 104 (2) ◽

pp. 183-191 ◽

Cited By ~ 105

Author(s):

Kenneth E. Lentz ◽

Leonard T. Skeggs ◽

Kenneth R. Woods ◽

Joseph R. Kahn ◽

Norman P. Shumway

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Ion Exchange ◽

Amino Acid Composition ◽

Acid Content ◽

Amino Acid Content ◽

Converting Enzyme ◽

Terminal Sequence ◽

Carboxyl Terminal ◽

Hydrolysis Of

Preparations of hypertensin II, obtained from the treatment of hypertensin I by the action of the hypertensin converting enzyme of plasma and purified by countercurrent distribution, were quantitatively analyzed for their amino acid content. Chromatography on ion exchange columns showed the presence of equimolar amounts of aspartic acid, proline, valine, isoleucine, tyrosine, phenylalanine, histidine, and arginine. Hypertensin I was found to contain one mole of leucine and one mole of histidine in addition to the amino acids of hypertensin II. These two amino acids were isolated from the conversion products of hypertensin I and identified as the peptide histidylleucine. Carboxypeptidase digestion of hypertensin I showed the carboxyl terminal sequence of amino acids to be residue-phenylalanyl-histidylleucine. Similar studies of hypertensin II demonstrated residue-phenylalanine. It was concluded that the conversion of hypertensin I by the plasma hypertensin converting enzyme involved hydrolysis of the phenylalanyl-histidine bond to form hypertensin II and histidylleucine. The further removal by carboxypeptidase of phenylalanine from hypertensin II destroyed all of the vasoconstrictor activity.

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COMPARISON OF AMINO ACIDS IN 8 DIFFERENT BOILED TROPICAL FRUITS

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2019v11i1.31995 ◽

2019 ◽

pp. 21-23

Author(s):

Radha Palaniswamy ◽

Dhanyasri Selvaraj ◽

Sandhiya Renganathan

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Acid Content ◽

Protein Quality ◽

Amino Acid Content ◽

Essential Amino Acids ◽

Tropical Fruits ◽

Good Balance

Objective: To determine the protein quality, especially the amino acid content of 8 tropical fruits both raw and boiled samples. Eight different tropical fruits were used in the study (Apricot, Jamun, Dragonfruit, Pomegranate, Mangustan, Litchi, Jackfruit, and Kiwi.Methods: Ninhydrin method was used for the estimation of the concentration of amino acids present in the above fruits. Raw and boiled fruits were used for the study.Results: Both raw and boiled forms which showed thats Jamun and Mangustan contained highest concentration amino acids whereas apricot shows the lowest concentration of amino acids except in Jamun which showed higher values in the raw fruit whereas in others the boiled samples showed higher values.Conclusion: It was evident that tropical fruits have a good balance of the essential amino acids (both raw and boiled fomr) which provide significant sources of protein in our diet.

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THE METABOLISM OF YEAST SPORULATION: VI. CHANGES IN AMINO ACID CONTENT DURING SPOROGENESIS

Canadian Journal of Microbiology ◽

10.1139/m64-082 ◽

1964 ◽

Vol 10 (5) ◽

pp. 623-631 ◽

Cited By ~ 10

Author(s):

C. Ramirez ◽

J. J. Miller

Keyword(s):

Saccharomyces Cerevisiae ◽

Amino Acids ◽

Amino Acid ◽

Acid Content ◽

Free Amino Acids ◽

Free Amino ◽

Amino Acid Content ◽

Proline Content ◽

Sporulation Medium ◽

Free Proline

During 6-day exposures of cells of Saccharomyces cerevisiae to acetate sporulation medium, the content of free amino acids declined to approximately one-third of that of vegetative cells, but proline was exceptional in that it increased conspicuously in amount. The content of combined amino acids also diminished to about one-third, ammonia was evolved, and amino acids (not including proline) passed out of the cells into the medium. When dihydroxyacetone replaced acetate in the sporulation medium, the results were similar except that the decline in content of free and combined amino acids was much greater, more ammonia was evolved, and only very small amounts of amino acids could be detected in the medium. Transfer of sporulated cells to growth medium led to an increase in the pool of free amino acids, except for proline, which declined in amount.In two other species of Saccharomyces the free proline content also increased on exposure to sporulation medium, but in Schizosaccharomyces pombe and Torulopsis famata no such increase was observed.

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