scholarly journals Original Article. In vitro evaluation of potato genotypes for resistance against bacterial soft rot (Pectobacterium carotovorum) – a new tool for studying disease resistance

2016 ◽  
Vol 57 (1) ◽  
pp. 1-8
Author(s):  
Sima Azadmanesh ◽  
Javad Mozafari ◽  
Nader Hasanzadeh ◽  
Cobra Moslemkhani
Keyword(s):  
Soft Rot ◽  
Bacterial Suspension ◽  
Post Inoculation ◽  
Pectobacterium Carotovorum ◽  
In Vitro Screening ◽  
Bacterial Soft Rot ◽  
Inoculation Techniques ◽  
Potato Collection ◽  
Rot Disease

Abstract In vitro screening techniques were used to evaluate 46 genotypes of Iranian potato collection for resistance to bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum (Pcc). One month old in vitro rooted potato plantlets were inoculated by two inoculation techniques under in vitro conditions: 1) sterile toothpicks dipped into bacterial suspension and pressed into the crown of plantlets and 2) the freshly cut crown of plantlets were dipped into bacterial suspension of 108 cfu ∙ ml-1 for 10 min. Typical soft rot disease symptoms, including the percentage of wilted leaves were recorded on inoculated plantlets 3, 6, 9, 12 and 15 days post-inoculation. The potato genotypes which were examined responded differently to Pcc and varying levels of resistance were observed. Potato genotype AG showed the highest level of resistance. Results obtained from in vitro screening were then verified by classical tuber slice assay. The verifications were conducted using five representative cultivars: Milva, Ramus, Picaso, Marfona and Agria which responded similarly to both in vitro and classical evaluation systems. Similar results obtained from these tests indicated that the in vitro screening technique developed in this study could provide a simple and rapid whole plant assay in selecting resistant potato genotypes against bacterial soft rot.

scholarly journals Biological control of Pectobacterium carotovorum subsp. carotovorum, the causal agent of bacterial soft rot in vegetables, in vitro and in vivo tests

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Hassan Abd-El-Khair ◽  
Tarek G. Abdel-Gaied ◽  
Maurice S. Mikhail ◽  
Ahmed I. Abdel-Alim ◽  
Hamdy I. Seif El-Nasr
Keyword(s):  
Potato Tuber ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Bacterial Soft Rot ◽  
Potato Tubers ◽  
Soft Rot Disease ◽  
In Vivo Tests ◽  
Rot Disease

Abstract Background Several chemical bactericides were applied for controlling soft rot bacteria, Pectobacterium carotovorum subsp. carotovorum, which causes the destructive soft rot disease to many economically important vegetables, but because of their toxic hazards on human and environment became limit. The biocontrol was applied to control many plant pathogens. Therefore, this work is aimed to study the antagonistic activity of bacterial agents, i.e. Bacillus subtilis, Bacillus pumilus, Bacillus megaterium and Pseudomonas fluorescens, and fugal agents, i.e. Trichoderma harzianum, Trichoderma viride and Trichoderma virens, to control bacterial soft rot disease under in vitro and in vivo tests. Results The tested treatments could protect the potato tubers against the development of soft rot. T. viride and T. virens were highly effective in reducing soft rot symptoms on inoculated potato tuber slices, when applied at the same time or 2 h before pathogen inoculation, while B. megaterium and T. harzianum were highly effective when applied at the same time or 2 h after pathogen inoculation. In whole potato tubers technique, B.pumilus highly protected the stored potato tuber under artificially infection conditions, than P. fluorescens, T. harzianum, B. subtilis, T. viride, T. virens and B. megaterium, respectively. Conclusion Application of fungal agents or specify the bacterial species can play an important role in controlling bacterial soft rot disease in vegetables and increase the stored periods of potato tubers under storage conditions without any toxic effects.

scholarly journals Effect of some chemicals on incidence of poato soft rot disease in Bangladesh

2017 ◽  
Vol 52 (2) ◽  
pp. 135-140 ◽  
Author(s):  
MM Rahman ◽  
AA Khan ◽  
IH Mian ◽  
AM Akanda ◽  
MZ Alam
Keyword(s):  
Acetic Acid ◽  
Boric Acid ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Potato Tubers ◽  
Soft Rot Disease ◽  
Bleaching Powder ◽  
Carotovorum Subsp ◽  
Rot Disease

Bactericidal effect was investigated by chemicals against potato soft rot bacteria in vitro and in storage. The chemicals were acetic acid, boric acid, bleaching powder, lactic acid, calcium hydroxide, calcium chloride, potassium chloride and sodium hypo-chloride. Among eight  chemicals only three chemicals viz. acetic acid, boric acid and bleaching powder showed bactericidal activity against potato soft rot bacteria  Pectobacterium carotovorum subsp. carotovorum (E. carotovora subsp. carotovora) P-138 in vitro. Based on the results of in vitro experiment three chemicals, acetic acid, boric acid and bleaching powder were used to control soft rot disease of potato in storage. Fresh potato tubers were dipped in 0.2% solution/suspensions of acetic acid, boric acid and bleaching powder for 30 min. Then soft rot bacteria Pectobacterium carotovorum subsp. carotovorum P-138 was inoculated on potato. Finally potatoes were stored for 22 weeks in net bags in sterilized condition. All the three chemicals significantly decreased the infection rate, loss in weight and increased percentage of disease reduction (PDR) of potato. Boric acid was the most effective in controlling the soft rot disease of potato in storage followed by acetic acid and bleaching powder. So these chemicals may be used for seed purpose storage of potato tubers for year round storage at farmer’s level.Bangladesh J. Sci. Ind. Res. 52(2), 135-140, 2017

scholarly journals First Report of Bacterial Soft Rot of Potato Caused by Pectobacterium carotovorum subsp. carotovorum in Guangdong Province of China

Plant Disease ◽  
2012 ◽  
Vol 96 (12) ◽  
pp. 1819-1819 ◽  
Author(s):  
J. X. Zhang ◽  
B. R. Lin ◽  
H. F. Shen ◽  
X. M. Pu ◽  
Z. N. Chen ◽  
...  
Keyword(s):  
Guangdong Province ◽  
Housekeeping Genes ◽  
Soft Rot ◽  
Bacterial Suspension ◽  
Pectobacterium Carotovorum ◽  
Bacterial Soft Rot ◽  
First Report ◽  
Wide Range ◽  
Carotovorum Subsp ◽  
Agar Media

Potato (Solanum tuberosum L.) is a major crop in China, with 80.0 million tons being produced in 2010 on 3.3 million ha. Pectobacterium carotovorum subsp. carotovorum Jones 1901; Hauben et al. 1999 causes soft rot worldwide on a wide range of hosts including potato, carrot, and cabbage. During spring 2010, a soft rot with a foul smell was noted in stored potato tubers of different cultivars in the Guangdong Province. Symptoms on tubers appeared as tan, water-soaked areas with watery ooze. The rotted tissues were white to cream colored. Stems of infected plants with typical inky black symptoms could also be found in the fields prior to harvest. Three different potato fields were surveyed, and 13% of the plants had the symptoms. Twenty-seven samples (three symptomatic tubers per sample) were collected. Bacteria were successfully isolated from all diseased tissues on nutrient agar media supplemented with 5% sucrose and incubated at 26 ± 1°C for 36 h. After purification on tripticase soy agar media, four typical strains (7-3-1, 7-3-2, 8-3-1, and 8-3-2) were identified using the following deterministic tests: gram-negative rods, oxidase negative, facultatively anaerobic, able to degrade pectate, sensitive to erythromycin, negative for phosphatase, unable to produce acid from α-methyl-glucoside, and produced acid from trehalose. Biolog analysis (Ver 4.20.05, Hayward, CA) identified the strains as P. carotovorum subsp. carotovorum (SIM 0.808, 0.774, 0.782, and 0.786, respectively). The identity of strains 7-3-1 (GenBank Accession No. JX258132), 7-3-2 (JX258133), and 8-3-1 (JX196705) was confirmed by 16S rRNA gene sequencing (4), since they had 99% sequence identity with other P. carotovorum subsp. carotovorum strains (GenBank Accession Nos. JF926744 and JF926758) using BLASTn. Further genetic analysis of strain 8-3-1 was performed targeting informative housekeeping genes, i.e., acnA (GenBank Accession No. JX196704), gabA (JX196706), icdA (JX196707), mdh (JX196708), mtlD (JX196709), pgi (JX196710), and proA (JX196711) (2). These sequences from strain 8-3-1 were 99 to 100%, homologous to sequences of multiple strains of P. carotovorum subsp. carotovorum. Therefore, strain 8-3-1 grouped with P. carotovorum subsp. carotovorum on the phylogenetic trees (neighbor-joining method, 1,000 bootstrap values) of seven concatenated housekeeping genes when compared with 60 other strains, including Pectobacterium spp. and Dickeya spp. (3). Pathogenicity of four strains (7-3-1, 7-3-2, 8-3-1, and 8-3-2) was evaluated by depositing a bacterial suspension (106 CFU/ml) on the potato slices of cultivar ‘Favorita’ and incubating at 30 ± 1°C. Slices inoculated with just water served as non-inoculated checks. The strains caused soft rot within 72 h and the checks had no rot. Bacteria were reisolated from the slices and were shown to be identical to the original strains based on morphological, cultural, and biochemical tests. Although this pathogen has already been reported in northern China (1), to our knowledge, this is the first report of P. carotovorum subsp. carotovorum causing bacterial soft rot of potato in Guangdong Province of China. References: (1) Y. X. Fei et al. J. Hexi Univ. 26:51, 2010.(2) B. Ma et al. Phytobacteriology 97:1150, 2007. (3) S. Nabhan et al. Plant Pathol. 61:498, 2012. (4) W. G. Weisbury et al. J. Bacteriol. 173:697, 1991.

scholarly journals In vitro efficacy of endophytic bacteria against Pectobacterium carotovorum subsp. carotovorum causing soft rot disease in banana

2021 ◽  
Vol 9 (1) ◽  
pp. 2087-2092
Author(s):  
R Kalaivanan ◽  
K Eraivan Arutkani Aiyanathan ◽  
S Thiruvudainambi ◽  
N Senthil ◽  
A Beaulah ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Soft Rot Disease ◽  
Carotovorum Subsp ◽  
Rot Disease

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

scholarly journals The Ribosomal Protein RplY Is Required for Pectobacterium carotovorum Virulence and Is Induced by Zantedeschia elliotiana Extract

2017 ◽  
Vol 107 (11) ◽  
pp. 1322-1330 ◽  
Author(s):  
Huan Jiang ◽  
Mengyi Jiang ◽  
Liuke Yang ◽  
Peiyan Yao ◽  
Lin Ma ◽  
...  
Keyword(s):  
Plant Extract ◽  
Plant Cell Wall ◽  
Insertion Site ◽  
Bacterial Pathogen ◽  
Soft Rot ◽  
Kanamycin Resistance ◽  
Pectobacterium Carotovorum ◽  
Promoter Trap ◽  
Carotovorum Subsp ◽  
Rot Disease

Pectobacterium carotovorum subsp. carotovorum strain PccS1, a bacterial pathogen causing soft rot disease of Zantedeschia elliotiana (colored calla), was investigated for virulence genes induced by the host plant. Using a promoter-trap transposon (mariner), we obtained 500 transposon mutants showing kanamycin resistance dependent on extract of Z. elliotiana. One of these mutants, PM86, exhibited attenuated virulence on both Z. elliotiana and Brassica rapa subsp. pekinensis. The growth of PM86 was also reduced in minimal medium (MM), and the reduction was restored by adding plant extract to the MM. The gene containing the insertion site was identified as rplY. The deletion mutant ΔrplY, exhibited reduced virulence, motility and plant cell wall-degrading enzyme production but not biofilm formation. Analysis of gene expression and reporter fusions revealed that the rplY gene in PccS1 is up-regulated at both the transcriptional and the translational levels in the presence of plant extract. Our results suggest that rplY is induced by Z. elliotiana extract and is crucial for virulence in P. carotovorum subsp. carotovorum.

scholarly journals Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽  
2016 ◽  
Vol 27 (3) ◽  
Author(s):  
Noor Istifadah ◽  
Muhamad Salman Umar ◽  
Sudarjat Sudarjat ◽  
Luciana Djaya
Keyword(s):  
Biological Control ◽  
Potato Tuber ◽  
Endophytic Bacteria ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Soft Rot Disease ◽  
Post Harvest ◽  
Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

scholarly journals Screening, Identification and Efficacy Evaluation of Antagonistic Bacteria for Biocontrol of Soft Rot Disease Caused by Dickeya zeae

2020 ◽  
Vol 8 (5) ◽  
pp. 697 ◽  
Author(s):  
Jieling Li ◽  
Ming Hu ◽  
Yang Xue ◽  
Xia Chen ◽  
Guangtao Lu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Prevention And Control ◽  
Pathogenic Bacteria ◽  
Soft Rot ◽  
Antagonistic Bacteria ◽  
Bacterial Soft Rot ◽  
Soft Rot Disease ◽  
Wide Range ◽  
Rot Disease ◽  
And Control

Dickeya zeae is the causal agent of bacterial soft rot disease, with a wide range of hosts all over the world. At present, chemical agents, especially agricultural antibiotics, are commonly used in the prevention and control of bacterial soft rot, causing the emergence of resistant pathogens and therefore increasing the difficulty of disease prevention and control. This study aims to provide a safer and more effective biocontrol method for soft rot disease caused by D. zeae. The spot-on-lawn assay was used to screen antagonistic bacteria, and three strains including SC3, SC11 and 3-10 revealed strong antagonistic effects and were identified as Pseudomonas fluorescens, P. parafulva and Bacillus velezensis, respectively, using multi-locus sequence analysis (MLSA) based on the sequences of 16S rRNA and other housekeeping genes. In vitro antimicrobial activity showed that two Pseudomonas strains SC3 and SC11 were only antagonistic to some pathogenic bacteria, while strain 3-10 had broad-spectrum antimicrobial activity on both pathogenic bacteria and fungi. Evaluation of control efficacy in greenhouse trials showed that they all restrained the occurrence and development of soft rot disease caused by D. zeae MS2 or EC1. Among them, strain SC3 had the most impressive biocontrol efficacy on alleviating the soft rot symptoms on both monocotyledonous and dicotyledonous hosts, and strain 3-10 additionally reduced the occurrence of banana wilt disease caused by Fusarium oxysporum f. sp. cubensis. This is the first report of P. fluorescens, P. parafulva and B. velezensis as potential bio-reagents on controlling soft rot disease caused by D. zeae.

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