scholarly journals Analytical strategy for determination of chloramphenicol in different biological matrices by liquid chromatography - mass spectrometry

2017 ◽  
Vol 61 (3) ◽  
pp. 321-327 ◽  
Author(s):  
Tomasz Śniegocki ◽  
Małgorzata Gbylik-Sikorska ◽  
Andrzej Posyniak
Keyword(s):  
Mass Spectrometry ◽  
Animal Origin ◽  
Organic Layer ◽  
Liquid Chromatography Mass Spectrometry ◽  
Biological Matrices ◽  
Analytical Strategy ◽  
Food Of Animal Origin ◽  
Main Target ◽  
Matrix Sample

AbstractIntroduction: The main problem in determination of chloramphenicol in food of animal origin is a large number of matrices. The main target of this study was to create a method for determination and confirmation of chloramphenicol in products and food of animal origin. Material and Methods: Each 5 g matrix sample was mixed with 5 mL of water and 10 mL of acetonitrile/ethyl acetate, homogenised, and centrifuged. The organic layer was evaporated and redissolved in 6 mL of 4% NaCl. The extract was cleaned up by SPE technique. Chloramphenicol was analysed by LC-MS/MS in electrospray mode. Results: The procedure was validated according to the Commission Decision No. 2002/657/EC. The apparent recoveries were in the range of 92.1% to 107.1% with a repeatability less than 11.0% (4.4%-11.0%) and within-laboratory reproducibility below 13.6% (4.7%-13.6%). Conclusion: The method was successfully validated and proved to be efficient, precise, and useful for quantification of chloramphenicol in more than 20 different matrices.

scholarly journals Recent advances in animal origin identification of gelatin-based products using liquid chromatography-mass spectrometry methods: A mini review

2020 ◽  
Vol 39 (1) ◽  
pp. 260-271
Author(s):  
Guiya Deng ◽  
Shangwei Guo ◽  
Fakhar Zaman ◽  
Tianyu Li ◽  
Yaqin Huang
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Animal Origin ◽  
Liquid Chromatography Mass Spectrometry ◽  
Functional Material ◽  
Connective Tissues ◽  
Chromatography Mass Spectrometry ◽  
Health Related ◽  
Origin Identification

Abstract Gelatin, a natural functional material obtained from animal connective tissues, has been broadly applied in health-related products such as food, pharmacy, and cosmetic. But unclear labelling and false information of animal origin of gelatin in those products would violate religious rules and increase public health risks. Recently, animal origin identification of gelatin-based products has drawn more and more concerns. Among various identification methods, liquid chromatography-mass spectrometry (LC-MS) has specifically become a research hotspot for animal origin identification and quantitative analysis of gelatin-based products due to its superior reliability, selectivity, and sensitivity. The main desideratum of the current treatise is to review the recent progress on this subject with respect to: (1) the identification of animal in halal gelatin-based products, (2) the determination of the authenticity of gelatinous medicines from animal, especially from the highly homologous family species, and (3) the quantification of gelatin in gelatin-based products, using LC-MS method. We hope that this review could provide theoretical guidance and advanced strategies for developing animal origin identification technologies for gelatin-based products.

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