scholarly journals Detection of CagA, VacA, IceA1 and IceA2 virulent genes in Helicobacter pylori isolated from gastric ulcer patients

2018 ◽  
Vol 42 (4) ◽  
pp. 155-162
Author(s):  
Lijuan Fan ◽  
Ran Li ◽  
Hongyun Li ◽  
Jian Zhang ◽  
Lingyun Wang
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Ulcer ◽  
Virulence Genes ◽  
East Asian ◽  
Virulence Gene ◽  
Gastric Biopsy ◽  
History Of ◽  
Male Patients ◽  
Polymerase Chain ◽  
H Pylori

Abstract Background Virulence factors of Helicobacter pylori including cagA, vacA, iceA and their association with clinical manifestation varied widely with different subpopulations. The objective of the study was to determine the prevalence of cagA, iceA1, iceA2, vacA, vacA s1/s2, vacA m1/m2, Western type cagA and East Asian type cagA virulence genes in H. pylori isolated from gastric ulcer patients and evaluate the association of these genes with gender, age, smoking and alcohol consumption. Methods Gastric biopsy samples from 172 patients were collected. H. pylori virulence genes, cagA, vacA, iceA1, iceA2, vacA s1/s2, vacA m1/m2, Western type cagA and East Asian type cagA were detected using polymerase chain reaction (PCR). Results Of the gastric biopsy samples collected, 48.3% of samples grew H. pylori. The vacA (68.7%) was the predominant virulence gene detected and associated with male patients and patients within the age group of 31–40 years. The cagA was the second most common gene detected and significantly associated with alcoholic patients. Conclusions H. pylori infection rate was 48.3% and was associated with patients who were smokers or had a history of smoking. The majority of our isolates were positive for any one of the virulence genes tested indicating that these isolates were highly virulent in nature.

scholarly journals Dissemination of cagA and cagE Virulence Genes Among H. Pylori Strains From Sudanese Patients With Gastric Discomfort

2021 ◽  
Author(s):  
Esraa Osman ◽  
Maram M. Elnosh ◽  
Aalaa Mahgoub Albasha ◽  
Amira A M Fadl ◽  
Luai Osman Ibrahim Marouf ◽  
...  
Keyword(s):  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Gastric Ulcer ◽  
Amino Acid ◽  
Virulence Genes ◽  
Protein Sequences ◽  
Pathogenicity Island ◽  
E Gene ◽  
Gastric Biopsies ◽  
H Pylori

Abstract ObjectivesHelicobacter pylori cytotoxin-associated gene pathogenicity island (cag-PAI) is one of the strain-specific genes (they do not exist in all strains). cag-PAI is involved in inducing inflammation, ulceration, and carcinogenesis. This study aimed to detect and characterize cagA and cagE virulence genes among H. pylori strains from Sudanese patients with gastric discomfort.ResultOut of 288 gastric biopsies screened for the presence of H. pylori, 34% (98/288) were positive, cagA gene was present in 41% (40/98) of specimens, mostly in patients with gastritis 72.5% (29/40), followed by duodenal ulcer 15% (6/40), esophagitis 5% (2/40), and 7.5% (3/40) in patients diagnosed normal by endoscopy. cagE gene was present in 39% (38/98) of specimens, the majority 73.7% (28/38) were from patients with gastritis, 10.5% (4/38) duodenal ulcer, 5.3% (2/38) esophagitis, 2.6% (1/38) gastric ulcer, and 7.9% (3/38) were diagnosed as normal. The cagA and cagE protein sequences have synonymous amino acid variations.

scholarly journals Transcriptional Profile of Helicobacter pylori Virulence Genes in Patients with Gastritis and Gastric Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Manouchehr Ahmadi Hedayati ◽  
Saeed Salavati
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Gastric Carcinoma ◽  
Clinical Outcomes ◽  
Virulence Genes ◽  
Gastric Biopsy ◽  
Transcriptional Profile ◽  
Statistical Correlations ◽  
H Pylori ◽  
Antral Biopsy

Introduction. Numerous molecular epidemiology studies have been performed about the frequency of Helicobacter pylori virulence genes in patients with H. pylori infection so far. This study was conducted to detect transcriptional profile by cDNA of H. pylori virulence genes in gastric biopsy samples of gastritis and gastric carcinoma patients. Materials and Methods. In a case-control study, based on the prevalence of gastritis and gastric cancer in Sanandaj city during 2018 and 2019, 23 and 11 gastric antral biopsy samples with H. pylori infection were collected from gastritis and gastric carcinoma patients by the consecutive and available sampling method. Pathological characters, including tumor grades and tumor areas for gastric carcinoma biopsy samples prepared from gastric cancer areas, were determined by the pathologist. Total RNA of gastric antral biopsy samples was extracted, and their cDNA was synthesized by TaKaRa kit. H. pylori virulence genes’ cDNA using specific primers and PCR was detected. This study’s results were analyzed by SPSS version 25 and statics chi-square tests for determination of relationship and correlation between cDNAs of H. pylori transcriptional profile and clinical outcomes of H. pylori infection, including gastritis, gastric carcinoma, tumor grades, and tumor area. Results. The positive statistical correlations were observed between transcripts of cagA, cagA-EPIYAC, cagE, and cagY genes and H. pylori infection clinical outcomes ( P < 0.05 ). Conclusion. Detection of the H. pylori virulence genes’ cDNA in gastric biopsy samples can help provide the prognosis of clinical outcomes.

scholarly journals Genotyping of Helicobacter pylori Virulence Genes cagA and vacA: Regional and National Study

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Rania M. Kishk ◽  
Nashaat M. Soliman ◽  
Maha M. Anani ◽  
Nader Nemr ◽  
Ayman Salem ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Virulence Genes ◽  
Histopathological Examination ◽  
Virulence Gene ◽  
National Study ◽  
Rapid Urease Test ◽  
Urease Test ◽  
Cytotoxin Associated Gene A ◽  
H Pylori

Helicobacter pylori (H. pylori) plays a crucial role in the pathogenesis of gastritis, peptic ulcer, and gastric cancer. The presence of pathogenicity islands (PAI) genes contributes to the pathogenesis of many gastrointestinal disorders. Cytotoxin-associated gene A (cagA) and vacuolating cytotoxin gene (vacA) are the most known virulence genes in H. pylori. So, our aim was to study H. pylori virulence genes’ role in gastric disorders pathogenesis. Our study included 150 adult patients who suffered dyspeptic symptoms and were referred to the GIT endoscopy unit. Gastric biopsies were attained for rapid urease test (RUT) and histopathological examination, and multiplex PCR technique for detection of virulence genes was performed. It was found that 100 specimens were (RUT) positive, of which sixty samples (60%) were PCR positive for H. pylori ureC gene. The vacA and cagA genes were identified in 61.6% and 53% of H. pylori strains, respectively. Only 5 cases were vacA-positive and cagA-negative. The most virulent vacA s1 allele existed in 56.6% of cases. Out of the 60 H. pylori strains, 66% had at least one virulence gene and 34% did not show any virulence gene. H. pylori infection showed significant increase with age. H. pylori are prevalent amid dyspeptic patients in our region. The main genotype combinations were vacA+/cagA+ of s1m1 genotype and they were frequently associated with peptic ulcer diseases, gastritis, and gastroesophageal reflux disease.

scholarly journals Correlation between virulence markers of Helicobacter pylori in the oral cavity and gastric biopsies

2017 ◽  
Vol 54 (3) ◽  
pp. 217-221 ◽  
Author(s):  
Myriam Lucrecia MEDINA ◽  
Marcelo Gabriel MEDINA ◽  
Luis Antonio MERINO
Keyword(s):  
Helicobacter Pylori ◽  
Oral Cavity ◽  
Virulence Genes ◽  
Gastric Biopsy ◽  
Oral Diseases ◽  
Cross Sectional ◽  
Virulence Markers ◽  
Significant Difference ◽  
Gastric Biopsies ◽  
H Pylori

ABSTRACT BACKGROUND: The clinical outcome of Helicobacter pylori infection has been associated with virulence factors. The presence of these factors is useful as molecular markers in the identification of the high risk for developing severe gastric pathologies. OBJECTIVE: To correlate the presence of virulence markers cagA and bab2A of H. pylori in oral and gastric biopsy samples. METHODS: An observational, prospective, descriptive, and cross-sectional study was carried out between September 2011 and September 2012. Patients suffering dyspepsia with indication for upper gastrointestinal video endoscopy who attended the Gastroenterology Service of the Hospital Dr. Julio C. Perrando were included. Epidemiological investigation was completed. To detect the bacteria and their virulence genes, samples of saliva, dental plaque and gastric biopsy were taken and processed by PCR. RESULTS: Sixty-one patients were selected for this study (30 women and 31 men). H. pylori was detected in 31 gastric biopsies and 31 oral samples. Significant difference between oral and gastric samples was found in cagA genotype. Agreement between oral and gastric genotypes was found in 38.7% of samples from the same patient. CONCLUSION: This study is the first in provide information about the genotypes of the Argentinean Northeast H. pylori strains. Despite the high prevalence of H. pylori infection, the most of patients had less virulent genotypes in oral cavity and gastric tissue. The cagA / babA2 combination was not frequent in the samples studied. There was not a statistical correlation between the virulence genes and gastroduodenal or oral diseases. Although in some patients the same genotype was found both in oral and gastric samples, it cannot be ensure that they corresponding to the same strain because a DNA sequencing was not performed.

The Effect of Helicobacter pylori Eradication on the Gastrointestinal Microbiota in Patients with Duodenal Ulcer

2016 ◽  
Vol 25 (2) ◽  
pp. 139-146 ◽  
Author(s):  
Lin Li ◽  
Xiaoying Zhou ◽  
Shuping Xiao ◽  
Feng Ye ◽  
Guoxin Zhang
Keyword(s):  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Eradication Therapy ◽  
Gastrointestinal Microbiota ◽  
Helicobacter Pylori Eradication ◽  
Male Patients ◽  
Polymerase Chain ◽  
H Pylori ◽  
Number Of Bacteria ◽  
Control Study

Background: Recent reports have indicated that Helicobacter pylori (H. pylori) might have an effect on gastrointestinal flora; moreover, gastric commensual bacteria have been observed in the development of duodenal ulcer (DU). Aims: In our study, we aimed to evaluate the effect of H. pylori eradication on gastrointestinal flora in DU patients. Methods: A case-control study was performed at Jiangsu Shengze Hospital between December, 2013 and April, 2014. The patients received antibiotic eradication therapy if H. pylori testing was positive. At least four weeks after cessation of the eradication therapy, a repeat gastroscopy was performed to collect biopsies again in the same position. Gastric mucosa samples and feces specimens were collected to extract bacteria DNA and then to quantify by real-time polymerase chain reaction (PCR). Results: After the eradication of H. pylori, an increase of Lactobacillus group, Clostridium leptum subgroup, Enterobacteria and a decrease of Clostridium coccoides subgroup were found in the antrum. In the corpus, the number of bacteria in the Lactobacillus group was increased and the expression of Clostridium coccoides subgroup was significantly down-regulated. In the feces samples, only the number in the Lactobacillus group was increased. Moreover, the distribution was significantly different between female and the male patients. Conclusions: The presence of H. pylori in the stomach suppressed the colonization with Lactobacillus group, Clostridium leptum subgroup and Enterobacteria. Gender might affect the distribution and/or recolonization of the bacteria in DU patients. Abbreviations: DU: duodenal ulcer; GI: gastrointestinal; GIN: gastrointestinal neoplasia; GU: gastric ulcer; PPI: proton pump inhibitors; UBT: urea breath test.

scholarly journals Determination of Helicobacter pylori Virulence Genes in Gastric Biopsies by PCR

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Tamer Essawi ◽  
Wail Hammoudeh ◽  
Israr Sabri ◽  
Walid Sweidan ◽  
Mohammad A. Farraj
Keyword(s):  
Helicobacter Pylori ◽  
Virulence Genes ◽  
Gastric Biopsy ◽  
Strong Indication ◽  
Specific Primers ◽  
Biopsy Specimens ◽  
Pcr Identification ◽  
Gastric Biopsies ◽  
H Pylori

Aim. The aim of this study was to identify the presence of H. pylori in biopsy specimens from symptomatic patients by PCR. In addition, the rate of cagA, vacA, iceA1, and iceA2 virulence genes was determined. Materials and Methods. One hundred antral gastric biopsy specimens were collected during endoscopy from patients suffering from gastroduodenal symptoms. The samples were collected by the gastroenterologists in their own clinics in Ramallah, Palestine. DNA was extracted from the biopsies and subsequently used for PCR identification of H. pylori and the virulence genes using specific primers. Results. The rate of positive H. pylori in the collected biopsies was 44%. The rates of the virulence genes in this sample: cagA, vacA, iceA1, and iceA2 were 65.9%, 40.9%, 63.6%, and 84.1%, respectively. Conclusion. The iceA2 gene was the most frequent in this study. Much research is necessary to determine the presence of an association of this gene with gastric pathology. Variation in the rates of the iceA gene in different countries is a strong indication of its geographical distribution. This study would provide important information regarding the prevalence of virulence genes (vacA, cagA, iceA1, and iceA2) in H. pylori strains in the sample tested in this country.

scholarly journals Effects of periodontal therapy on eradication and recurrence of Helicobacter pylori infection after successful treatment

2019 ◽  
Vol 47 (2) ◽  
pp. 875-883 ◽  
Author(s):  
Taweesak Tongtawee ◽  
Wareeporn Wattanawongdon ◽  
Theeraya Simawaranon
Keyword(s):  
Helicobacter Pylori ◽  
Virulence Genes ◽  
Eradication Rate ◽  
Therapy Group ◽  
Periodontal Therapy ◽  
Chain Reaction ◽  
Helicobacter Pylori Eradication ◽  
Close Relationship ◽  
Polymerase Chain ◽  
H Pylori

Objectives This study aimed to evaluate the effects of periodontal therapy on the efficacy of Helicobacter pylori eradication and on the recurrence of infection after eradication. Methods We conducted a prospective randomized trial on 698 gastric H. pylori-infected patients, of whom 347 received gastric H. pylori treatment alone and 342 received gastric H. pylori treatment plus periodontal therapy. The presence of H. pylori and associated virulence genes were detected by real-time polymerase chain reaction. Results After eradication of gastric H. pylori infection, the recurrence of gastric H. pylori was significantly lower in the gastric H. pylori treatment plus periodontal therapy group than in the group receiving gastric H. pylori treatment alone (OR 0.67; 95% CI 0.45 to 0.99), whereas the eradication rate was not significantly different (OR 0.87; 95% CI 0.68 to 0.98). There was a close relationship between the presence of H. pylori in saliva and its presence in the stomach. Conclusions The oral cavity is an important reservoir for gastric H. pylori infection. Adjunctive periodontal therapy could enhance the efficiency of H. pylori treatment and reduce the recurrence of gastric H. pylori infection.

scholarly journals Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan

2013 ◽  
Vol 7 (03) ◽  
pp. 220-228 ◽  
Author(s):  
Adnan Khan ◽  
Amber Farooqui ◽  
Yasir Raza ◽  
Faisal Rasheed ◽  
Hamid Manzoor ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Disease Burden ◽  
Detection Rate ◽  
East Asian ◽  
Disease Association ◽  
Gastric Biopsy ◽  
Caga Gene ◽  
H Pylori ◽  
Type Strains

Introduction: The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of H. pylori genotypes and their association with different clinical outcomes among dyspeptic patients in Pakistan over a period of four years. Methodology: Gastric biopsy samples from a total of 450 dyspeptic individualswere subjected to PCR, genotypingand histology. Results: A total of 201 (45%) cases were found positive for H. pylori. The detection rate was high in GU (91%), DU (86%) and GC (83%) cases compared with those cases who had intact gastric mucosa (18%). Histology revealed the presence of infection in 68% of cases of mild/chronic nonspecific gastritis with others belonging to the GU sequel. cagA gene carriage was observed in 104 (51%) cases or mostly from DU, GU and GC groups, of which 97 were Western type strains while 3 were East-Asian type strains that are rarely observed in South Asia. vacA allelic variant s1am1 was most commonly observed, followed by s1am2, and s1bm1, with direct correlation in diseased cases (gastritis, GU, DU and GC). Prevalent genotypic combinations were s1am1/cagA- in gastritis and s1am1/cagA+ in DU, GU, and GC. Conclusions: Our study indicates the predominant circulation of Western type cagA and vacAs1am1 type H. pylori strains in Pakistan.

scholarly journals Prevalence of Helicobacter pylori infection in patients with gastric ulcer perforation admitted in a tertiary care centre

2019 ◽  
Vol 6 (3) ◽  
pp. 898
Author(s):  
Muhammed Rashim P. ◽  
S. Vineed
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Ulcer ◽  
Tertiary Care ◽  
Gastric Ulcers ◽  
Care Centre ◽  
Tertiary Care Centre ◽  
Ulcer Perforation ◽  
History Of ◽  
Perforation Peritonitis ◽  
H Pylori

Background: Gastric ulcers are one of the most prevalent gastro intestinal diseases. Perforation of gastric ulcer is most common and dreaded complication of a gastric ulcer. Causes of gastric ulcer include Helicobacter Pylori, the NSAID, smoking and alcohol. Helicobacter Pylori infection is a curable cause of gastric ulcer. As prevalence of H. pylori differ in populations, prevalence of H. pylori in our population need to be assessed for determining treatment strategy for gastric ulcer.Methods: Cross sectional study conducted in patients operated for perforation peritonitis and diagnosed to have gastric ulcer perforation in a tertiary care centre. Full thickness biopsy was taken from gastric ulcer perforation edge during the surgery for perforation peritonitis. The biopsy was stained with Giemsa stain and looked for the presence of H. pylori.Results: Helicobacter pylori prevalence was 48.8% in our study. 8 females (out of 16) and 51 males (out of 105) had H. pylori positivity. 43 had history of smoking. Among them 20 were diagnosed to be having H. pylori positive. 26 persons had history of pan chewing. Among pan chewers 17 were H. pylori positive. Among 50 patients with history of alcoholism, 24 got H. pylori positivity. Among 40 patients with history of NSAIDs, 14 were positive for H. pylori.Conclusions: Prevalence of Helicobacter pylori in gastric ulcer perforation in present study is 48.8%. Helicobacter Pylori eradication should be added in treatment protocols for perforated gastric ulcers.

scholarly journals Disappearance of Helicobacter without Antibiotics in 12 Patients with Gastritis

1997 ◽  
Vol 11 (2) ◽  
pp. 167-172 ◽  
Author(s):  
Hugh James Freeman
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Natural History ◽  
Gastric Biopsy ◽  
Endoscopic Procedures ◽  
History Of ◽  
Gastric Biopsies ◽  
H Pylori ◽  
Upper Gastrointestinal

Detection ofHelicobacter pyloriin endoscopic gastric biopsies has been associated with a variety of diseases, including ulcers and gastritis. Although the natural history ofH pyloriin the gastric mucosa is unknown, antibiotic regimens have been used for eradication. Gastric biopsies from 6050 endoscopic procedures done by a single gastroenterologist from 1981 to 1994 were evaluated. Of these, 2860 from April 1, 1991 to September 30, 1994 had silver-stained biopsies to facilitateH pyloridetection, and at least two upper endoscopic procedures were done with gastric biopsies in 188 patients. Twelve of the 188 patients with an initially positiveH pylorigastric biopsy becameH pylori-negative without antibiotic treatment forH pylorior other infection; 10 received omeprazole and two received no drug treatment. In two of the 12 patients recurrentH pyloriin the gastric mucosa was also documented. These findings indicate thatH pylorimay disappear and reappear in the gastric mucosa with no specific antibiotic eradication regimen, although omeprazole may eradicateH pyloriin vivo in some patients. The natural history ofH pyloriin gastric biopsies is poorly understood. Improved understanding, especially regarding the pathogenesis of upper gastrointestinal ulcerative and inflammatory disease processes, is essential before recommendations for specific antibiotic eradication regimens can be made.

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