Analysis of conditions for the preparation of BSA/MMT composites

2013 ◽  
Vol 20 (3) ◽  
pp. 221-226 ◽  
Author(s):  
Yinghai Lv ◽  
Xiaolu Su ◽  
Fuli Ren ◽  
Hong-qiang Kan ◽  
Ying Kong
Keyword(s):  
Ionic Strength ◽  
Acetate Buffer ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Experimental Conditions ◽  
Ph Values ◽  
Fixation Effect ◽  
The Impact ◽  
Adsorption Quantity ◽  
Better Than

AbstractThis study describes a new method for protein fixation that has high, stable, and good repeatable fixation effect without deactivating protein. This method involves intercalating bovine serum albumin (BSA) into montmorillonite (MMT) under different experimental conditions, i.e., the concentration of coupling agent KH570, pH values, ionic strength, and the impact of buffers. The study showed the interaction of buffer systems with pH values had the most significant influence on adsorption quantity. The existence of the buffer increased the adsorption quantity and helped with slow protein releasing, while the phosphate buffer worked better than acetate buffer. More BSA was adsorbed on KH570 treated than untreated MMT, and among the treated samples the most protein released was on MMT treated with KH570 in 100% ethanol and in the acetate buffer solution. Adsorption quantity of the BSA initially increased and then decreased with the increase of the ionic strength. The research on the preparation of MMT/BSA materials provides further information on the study of protein composites.

Enzymatic hydrolysis of chitosan in acetate buffer solution

2015 ◽  
Vol 88 (4) ◽  
pp. 647-651 ◽  
Author(s):  
V. V. Chernova ◽  
A. S. Shurshina ◽  
M. V. Bazunova ◽  
E. I. Kulish
Keyword(s):  
Enzymatic Hydrolysis ◽  
Acetate Buffer ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Hydrolysis Of

Collaborative Study of an Ultraviolet Method for Measuring Diquat Content in Formulations

1968 ◽  
Vol 51 (6) ◽  
pp. 1304-1305
Author(s):  
A A Carlstrom
Keyword(s):  
Systematic Error ◽  
Random Error ◽  
Coefficient Of Variation ◽  
Collaborative Study ◽  
Acetate Buffer ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Standard Curve ◽  
Two Samples

Abstract The ultraviolet method for diquat described by Yuen, Bagness, and Myles in 1967 was collaboratively studied with formulations containing 2 lb diquat/gal. A portion of the sample is diluted with an acetate buffer solution and the absorbance is measured at 310 mμ the diquat content is obtained by reference to a standard curve prepared from known diquat concentrations. Single determinations on two samples by thirteen collaborators show an overall coefficient of variation of 2.4%. The coefficient of variation for random error is 1.0%, and for systematic error 1.6%. The method is recommended for adoption as official, first action.

Preparation and characterization of a poly-o-phenylenediamine film modified glassy carbon electrode as a H2O2 sensor

2013 ◽  
Vol 91 (11) ◽  
pp. 1077-1084 ◽  
Author(s):  
Wenying Zhai ◽  
Xiuying Tian ◽  
Yun Yan ◽  
Yuehua Xu ◽  
Yuechun Zhao ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Electrochemical Impedance ◽  
Monomer Concentration ◽  
Acetate Buffer ◽  
Acetate Buffer Solution ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Buffer Solution

A poly-o-phenylenediamine film modified glassy carbon electrode (PoPD/GC) was successfully prepared by cyclic voltammetry in acetate buffer solution. The polymerization mechanism of oPD is discussed. The impedance behavior and morphology of the PoPD membrane were characterized using cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy, respectively. It is discovered that the PoPD/GC prepared only in acetate buffer solution had dual electrocatalytic activity toward the oxidation and reduction of H2O2. The optimal buffer solution pH, scanning rate, monomer concentration, and number of scannings for film forming were 4.2, 0.05 V s−1, 6.0 mmol L−1, and 30, respectively. The linear ranges between the anodic (Δia) or cathodic (Δic) current and H2O2 concentration were 0.07−1.0 × 104 and 0.04−4.5 × 104 μmol L−1, respectively. The corresponding calibration curves were Δia (μA) = 8.03c (mmol L−1) + 6.36 (n = 18, R2 = 0.9989) and Δic (μA) = −5.52c (mmol L−1) − 0.77 (n = 18, R2 = 0.9990) with a detection limit of 0.03 and 0.02 μmol L−1 (S/N = 3), respectively. The PoPD/GC prepared in the optimal conditions showed good stability and quick response (<0.2 s) to H2O2, which was successfully applied to the determination of H2O2 in real water samples with satisfactory results.

scholarly journals Development of Amperometric Laccase Biosensor through Immobilizing Enzyme in Magnesium-Containing Mesoporous Silica Sieve (Mg-MCM-41)/Polyvinyl Alcohol Matrix

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Z. Dai ◽  
M. Q. Guo ◽  
X. J. Wang ◽  
H. F. Wang ◽  
W. Y. Chen
Keyword(s):  
Mesoporous Silica ◽  
High Surface ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Experimental Conditions ◽  
Surface Areas ◽  
Long Time ◽  
Good Biocompatibility ◽  
Laccase Immobilization ◽  
Mcm 41

Magnesium-containing mesoporous silica sieve (Mg-MCM-41) provided a suitable immobilization of biomolecule matrix due to its uniform pore structure, high surface areas, fast electron-transfer rate, and good biocompatibility. Based on this, an amperometric biosensor was developed by entrapping laccase into the Mg-MCM-41/PVA composite matrix. Laccase from Trametes versicolor was assembled on a composite film of Mg-MCM-41/PVA modified Au electrode and the electrode was investigated by cyclic voltammetry, impedance spectroscopy, and chronoamperometry. The results indicated that the Mg-MCM-41/PVA/Lac modified electrode exhibited excellent catalytic activity towards catechol at room temperature in pH 4.8 acetate buffer solution. The optimum experimental conditions of biosensor for the detection of catechol were studied in detail. Under the optimal conditions, the linear range was from 0.94 to 10.23 μM with the sensitivity of 16.9227 A/M, the detection limit of 0.00531 μM, and the response time of less than 14 s. The Michaelis-Menten constant (KMapp) was estimated by Lineweaver-Burk equation and theKMappvalue was about 1.01 μM. In addition, the biosensor exhibited high reproducibility and long-time stability. This work demonstrated that Mg-MCM-41/PVA composite provides a suitable support for laccase immobilization and construction of biosensor.

scholarly journals Interaction of Manganese(II) with Proton Pump Inhibitor at Different pH: A Cyclic Voltammetric Study

2016 ◽  
Vol 64 (2) ◽  
pp. 115-120
Author(s):  
R Biswas ◽  
S Islam ◽  
MS Rahman ◽  
MA Haque ◽  
M Abu Hasan ◽  
...  
Keyword(s):  
Proton Pump ◽  
Suitable Condition ◽  
Acetate Buffer ◽  
Acetate Buffer Solution ◽  
Molar Ratio ◽  
Cyclic Voltammograms ◽  
Buffer Solution ◽  
Cyclic Voltammetric ◽  
Cyclic Voltammetric Study ◽  
Insight Into

The electrochemistry of Mn2+ at different pH (3.5-4.5) in acetate buffer solution has been investigated, using cyclic voltammetric method at glassy carbon electrode (GCE). Cyclic voltammograms (CVs) of Mn2+ show two cathodic and an anodic peaks over the entire investigated pH. The CVs of Mn2+ has also been performed in presence of proton pump inhibitors (PPIs) such as omeprazole, pantoprazole, esomeprazole and rabeprazole to elucidate the interaction of Mn2+ with the PPIs in acetate buffer solution. At the studied pH, a reasonably strong interaction was observed between Mn2+ and PPIs at different molar ratio of Mn2+ ion and PPIs. However, maximum interaction has been found at 1:2 molar ratio of Mn2+ and PPIs. This indicates the most suitable condition for the interaction between Mn2+ and PPIs. These results could allow insight into the interactions of metal ions with the PPIs. Dhaka Univ. J. Sci. 64(2): 115-120, 2016 (July)

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