Three-Dimensional Electron Microscopy of Individual Biological Objects Part III. Experimental Results on Yeast Fatty Acid Synthetase

1976 ◽  
Vol 31 (11) ◽  
pp. 1380-1390 ◽  
Author(s):  
W. Hoppe ◽  
H. J. Schramm ◽  
M. Sturm ◽  
N. Hunsmann ◽  
J. Gaßmann
Keyword(s):  
Molecular Structure ◽  
Fatty Acid ◽  
Point Group ◽  
Three Dimensional ◽  
Fatty Acid Synthetase ◽  
Experimental Results ◽  
Dose Reconstruction ◽  
Dimensional Electron ◽  
Biological Objects ◽  
Symmetry Relations

Abstract Experimental results of three-dimensional reconstructions of individual fatty acid synthetase (FAS) molecules are reported. The discussion of the structures has been concentrated on the symmetry relations within the molecules. A molecular structure with 6 copies arranged in point group 32 is probable. The limits of minimal radiation dose reconstruction are estimated.

Three-Dimensional Electron Microscopy of Individual Biological Objects Part II. Test Calculations

1976 ◽  
Vol 31 (11) ◽  
pp. 1370-1379 ◽  
Author(s):  
W. Hoppe ◽  
H. J. Schramm ◽  
M. Sturm ◽  
N. Hunsmann ◽  
J. Gaßmann
Keyword(s):  
Electron Microscopy ◽  
Computer Simulations ◽  
Three Dimensional ◽  
Image Point ◽  
Dimensional Electron ◽  
Biological Objects ◽  
Shape Variable

Abstract In this second part of the paper the general conclusions of part I with regard to resolution and image point shape, variable boundaries of the projected three-dimensional body and origin definition have been tested by computer simulations. The influence of clutter and noise has been discussed.

Molecular structure and expression of fatty acid synthetase genes in yeast

1986 ◽  
Vol 14 (3) ◽  
pp. 572-574 ◽  
Author(s):  
MICHAEL SCHWEIZER ◽  
LILIAN M. ROBERTS ◽  
JOACHIM HÖLTKE ◽  
KENJI TAKABAYASHI ◽  
GERHARD MÜLLER ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Fatty Acid ◽  
Fatty Acid Synthetase

Three-Dimensional Electron Microscopy of Individual Biological Objects

1976 ◽  
Vol 31 (6) ◽  
pp. 645-655 ◽  
Author(s):  
W. Hoppe ◽  
H. J. Schramm ◽  
M. Sturm ◽  
N. Hunsmann ◽  
J. Gaßmann
Keyword(s):  
Electron Microscopy ◽  
Three Dimensional ◽  
Image Point ◽  
Two Dimensional ◽  
Dimensional Electron ◽  
Biological Objects ◽  
Dimensional Reconstruction ◽  
The Common ◽  
Measuring Scheme

In this paper methods and results of three-dimensional electron microscopy of individual molecules will be presented. Part I describes the general experimental and theoretical methods (microgoniometer, measuring scheme, two-dimensional and three-dimensional reconstruction, determination of the common origin of the projections). Special attention will be given to the image point shapes under different reconstruction conditions

scholarly journals Distribution of yeast fatty acid synthetase subunits: three-dimensional model of the enzyme.

1978 ◽  
Vol 75 (12) ◽  
pp. 5792-5796 ◽  
Author(s):  
F. Wieland ◽  
E. A. Siess ◽  
L. Renner ◽  
C. Verfurth ◽  
F. Lynen
Keyword(s):  
Fatty Acid ◽  
Three Dimensional ◽  
Fatty Acid Synthetase ◽  
Dimensional Model ◽  
Three Dimensional Model

High Resolution Microscopy of Multi-Layered Biological Crystals

1982 ◽  
Vol 40 ◽  
pp. 80-83
Author(s):  
H.A. Cohen ◽  
T.W. Jeng ◽  
W. Chiu
Keyword(s):  
High Resolution ◽  
Low Dose ◽  
Three Dimensional ◽  
Data Interpretation ◽  
Two Dimensional ◽  
Biological Objects ◽  
Density Maps ◽  
Density Map ◽  
Complex Crystal ◽  
High Resolution Microscopy

This tutorial will discuss the methodology of low dose electron diffraction and imaging of crystalline biological objects, the problems of data interpretation for two-dimensional projected density maps of glucose embedded protein crystals, the factors to be considered in combining tilt data from three-dimensional crystals, and finally, the prospects of achieving a high resolution three-dimensional density map of a biological crystal. This methodology will be illustrated using two proteins under investigation in our laboratory, the T4 DNA helix destabilizing protein gp32*I and the crotoxin complex crystal.

Crystal Structure Analysis of Cu-Zr Alloys by Convergent Beam Diffraction

1981 ◽  
Vol 39 ◽  
pp. 354-355
Author(s):  
A. F. Marshall ◽  
J. W. Steeds ◽  
D. Bouchet ◽  
S. L. Shinde ◽  
R. G. Walmsley
Keyword(s):  
Crystal Structure ◽  
Point Group ◽  
Intermetallic Phase ◽  
Three Dimensional ◽  
Crystal Structure Analysis ◽  
Ion Milling ◽  
Composition And Structure ◽  
Unit Cells ◽  
Sputter Deposited ◽  
Convergent Beam

Convergent beam electron diffraction is a powerful technique for determining the crystal structure of a material in TEM. In this paper we have applied it to the study of the intermetallic phases in the Cu-rich end of the Cu-Zr system. These phases are highly ordered. Their composition and structure has been previously studied by microprobe and x-ray diffraction with sometimes conflicting results.The crystalline phases were obtained by annealing amorphous sputter-deposited Cu-Zr. Specimens were thinned for TEM by ion milling and observed in a Philips EM 400. Due to the large unit cells involved, a small convergence angle of diffraction was used; however, the three-dimensional lattice and symmetry information of convergent beam microdiffraction patterns is still present. The results are as follows:1) 21 at% Zr in Cu: annealed at 500°C for 5 hours. An intermetallic phase, Cu3.6Zr (21.7% Zr), space group P6/m has been proposed near this composition (2). The major phase of our annealed material was hexagonal with a point group determined as 6/m.

Improved 3-D reconstruction using stereo computer graphics and multiple tilt EM images

1995 ◽  
Vol 53 ◽  
pp. 630-631
Author(s):  
Lee D. Peachey ◽  
Lou Fodor ◽  
John C. Haselgrove ◽  
Stanley M. Dunn ◽  
Junqing Huang
Keyword(s):  
Computer Graphics ◽  
High Performance ◽  
Transverse Section ◽  
Three Dimensional ◽  
Stereo Pair ◽  
3D Reconstructions ◽  
Video Cameras ◽  
Biological Objects ◽  
Microscope Images ◽  
High Performance Computer

Stereo pairs of electron microscope images provide valuable visual impressions of the three-dimensional nature of specimens, including biological objects. Beyond this one seeks quantitatively accurate models and measurements of the three dimensional positions and sizes of structures in the specimen. In our laboratory, we have sought to combine high resolution video cameras with high performance computer graphics systems to improve both the ease of building 3D reconstructions and the accuracy of 3D measurements, by using multiple tilt images of the same specimen tilted over a wider range of angles than can be viewed stereoscopically. Ultimately we also wish to automate the reconstruction and measurement process, and have initiated work in that direction.Figure 1 is a stereo pair of 400 kV images from a 1 micrometer thick transverse section of frog skeletal muscle stained with the Golgi stain. This stain selectively increases the density of the transverse tubular network in these muscle cells, and it is this network that we reconstruct in this example.

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