Eine Methode zur qualitativen und quantitativen Bestimmung von drei Juvenilhormonen von Insekten

Abstract A method is presented permitting the qualitative and quantitative determination of all three presently known hormones (JH1-3). The determination is based on the method of radioactive isotope dilution, whereby a very small known amount of tritium-labelled JH-1 is added to the ether extract of the particular species. The addition of radioactive JH-1 permits the isolation of all three hormones, because of their similar behaviour during the chosen work up. The quantitative determination was carried out by gas chromatography and the identification was confirmed with the help of retention-times and GC-MS combination. The method was checked by using an extract of Hyalophora cecropia. For the first time methyl 10,11-epoxy-3,7,11-trimethyl-2-trans-6-trans-dodecadienoate (JH-3) could also be identified as the juvenile hormone of Melolontha melolontha. In Vanessa io larvae, Tenebrio molitor larvae and adults and in Musca domestica larvae none of the three known hormones could be detected. The preparation of JH-1 labelled with tritium in the methyl group of the ester was accomplished with very high specific activity (4.34 Ci/mmol) of the tritiated acid with diazomethane.

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Effects of Heparin Oligosaccharides with High Affinity for Antithrombin III in Experimental Venous Thrombosis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657178 ◽

1982 ◽

Vol 47 (03) ◽

pp. 244-248 ◽

Cited By ~ 47

Author(s):

D P Thomas ◽

Rosemary E Merton ◽

T W Barrowcliffe ◽

L Thunberg ◽

U Lindahl

Keyword(s):

Antithrombin Iii ◽

Specific Activity ◽

High Specific Activity ◽

Factor Xa ◽

Blood Levels ◽

Thrombin Time ◽

High Affinity ◽

Very High

SummaryThe in vitro and in vivo characteristics of two oligosaccharide heparin fragments have been compared to those of unfractionated mucosal heparin. A decasaccharide fragment had essentially no activity by APTT or calcium thrombin time assays in vitro, but possessed very high specific activity by anti-Factor Xa assays. When injected into rabbits at doses of up to 80 ¼g/kg, this fragment was relatively ineffective in impairing stasis thrombosis despite producing high blood levels by anti-Xa assays. A 16-18 monosaccharide fragment had even higher specific activity (almost 2000 iu/mg) by chromogenic substrate anti-Xa assay, with minimal activity by APTT. When injected in vivo, this fragment gave low blood levels by APTT, very high anti-Xa levels, and was more effective in preventing thrombosis than the decasaccharide fragment. However, in comparison with unfractionated heparin, the 16-18 monosaccharide fragment was only partially effective in preventing thrombosis, despite producing much higher blood levels by anti-Xa assays.It is concluded that the high-affinity binding of a heparin fragment to antithrombin III does not by itself impair venous thrombogenesis, and that the anti-Factor Xa activity of heparin is only a partial expression of its therapeutic potential.

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Determination of Flavonoids Compounds of Three Species and Different Harvesting Periods in Crataegi folium Based on LC-MS/MS

Molecules ◽

10.3390/molecules26061602 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1602

Author(s):

Ya-Ping Guo ◽

Hong Yang ◽

Ya-Li Wang ◽

Xiao-Xiang Chen ◽

Ke Zhang ◽

...

Keyword(s):

Quantitative Analysis ◽

Qualitative Analysis ◽

Quantitative Method ◽

Total Content ◽

Qualitative And Quantitative Analysis ◽

Pharmacological Activities ◽

Time Saving ◽

Qualitative And Quantitative ◽

First Time

Crataegi folium have been used as medicinal and food materials worldwide due to its pharmacological activities. Although the leaves of Crataegus songorica (CS), Crataegus altaica (CA) and Crataegus kansuensis (CK) have rich resources in Xinjiang, China, they can not provide insights into edible and medicinal aspects. Few reports are available on the qualitative and quantitative analysis of flavonoids compounds of their leaves. Therefore, it is necessary to develop efficient methods to determine qualitative and quantitative flavonoids compounds in leaves of CS, CA and CK. In the study, 28 unique compounds were identified in CS versus CK by qualitative analysis. The validated quantitative method was employed to determine the content of eight flavonoids of the leaves of CS, CA and CK within 6 min. The total content of eight flavonoids was 7.8–15.1 mg/g, 0.1–9.1 mg/g and 4.8–10.7 mg/g in the leaves of CS, CA and CK respectively. Besides, the best harvesting periods of the three species were from 17th to 26th September for CS, from 30th September to 15th October for CA and CK. The validated and time-saving method was successfully implemented for the analysis of the content of eight flavonoids compounds in CS, CA and CK for the first time.

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Qualitative and quantitative determination of trace aldehydes and ketones in food preservative propionic acid for quality improvement

Analytical Methods ◽

10.1039/d1ay00742d ◽

2021 ◽

Author(s):

Yiwen Cao ◽

Shenjie Zhu ◽

Lin Zhang ◽

Qun Cui ◽

Haiyan Wang

Keyword(s):

Quality Improvement ◽

Quantitative Analysis ◽

Quantitative Determination ◽

Propionic Acid ◽

Qualitative And Quantitative Analysis ◽

Food Preservative ◽

Qualitative And Quantitative ◽

Aldehydes And Ketones ◽

Aldehyde Content

Aiming at the difficulty in qualitative and quantitative analysis of trace compositions in food preservative propionic acid, the trace compositions and the key components influencing the total aldehyde content in...

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Qualitative and quantitative determination of the iron-bearing phases in deep sea-nodules by Mössbauer spectroscopy

Physics and Chemistry of Minerals ◽

10.1007/bf00205267 ◽

1992 ◽

Vol 18 (8) ◽

pp. 517-521 ◽

Cited By ~ 2

Author(s):

V. Rusanov ◽

V. Angelov ◽

V. Jordanov ◽

S. Ormandjiev

Keyword(s):

Mössbauer Spectroscopy ◽

Quantitative Determination ◽

Deep Sea ◽

Mossbauer Spectroscopy ◽

Qualitative And Quantitative ◽

Mößbauer Spectroscopy ◽

Iron Bearing

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Qualitative and quantitative determination of porphyrins in urine, blood and feces

Clinical Biochemistry ◽

10.1016/s0009-9120(87)80038-3 ◽

1987 ◽

Vol 20 (4) ◽

pp. 290-291

Author(s):

M. Moss ◽

C.S.Y. Wong ◽

T. McMahon ◽

C. Cousins ◽

C. Jacklyn

Keyword(s):

Quantitative Determination ◽

Qualitative And Quantitative

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Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS

Journal of Natural Medicines ◽

10.1007/s11418-011-0560-8 ◽

2011 ◽

Vol 66 (1) ◽

pp. 102-108 ◽

Cited By ~ 11

Author(s):

Shihu Wei ◽

Peicheng Zhang ◽

Xizhi Feng ◽

Hiroyuki Kodama ◽

Changyuan Yu ◽

...

Keyword(s):

Quantitative Determination ◽

Qualitative And Quantitative ◽

Esi Ms

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Determination of Free and Bound Maleic Hydrazide Residues in Tobacco by High Performance Liquid Chromatography

Beiträge zur Tabakforschung International/Contributions to Tobacco Research ◽

10.2478/cttr-2013-0632 ◽

1994 ◽

Vol 16 (2) ◽

pp. 57-64 ◽

Cited By ~ 1

Author(s):

BS Dattilo ◽

S Gallo ◽

G Lionetti ◽

SG Rossi

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Quantitative Determination ◽

High Performance ◽

Maleic Hydrazide ◽

Dilute Solution ◽

Tobacco Leaves ◽

Qualitative And Quantitative ◽

Curve Method

AbstractA new method is described for the qualitative and quantitative determination of both free and bound maleic hydrazide residues in tobacco leaves and cigarette filler by high performance liquid chromatography. Analyses were carried out by hydrolyzing samples of ground tobacco with 4 N hydrochloric acid for 40 minutes under reflux followed by sample chromatography, running isocratic elutions with a dilute solution of phosphoric acid. The quantitative determination of maleic hydrazide was performed by light absorption at 320 nm, by the calibration curve method. Recoveries of maleic hydrazide added to tobacco samples were greater than 90 %. The detection limit of the method, determined on ground tobacco leaves, was at least 5 ppm. The results obtained by this procedure and by the ISO standard method no. 4876 are in good accordance.

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