Notes: Determination of the Cytotoxicity of Rumex crispus during the Vegetation Period Using a Brine Shrimp Bioassay

1995 ◽  
Vol 50 (5-6) ◽  
pp. 461-462
Author(s):  
L. Ömür ◽  
Ayşe Kuruüzüm
Keyword(s):  
Toxic Effect ◽  
Brine Shrimp ◽  
Vegetation Period ◽  
Rumex Crispus ◽  
Anthraquinone Glycosides

The percentage of anthraquinone glycosides in the roots of Rumex crispus L. (Polygonaceae) is significantly greater than in other parts of the plant. The roots of R. crispus in different vegetation stages were investigated with regard to anthraquinone glycoside content. Their toxicity towards shrimp were also tested during the vegetation period. The data showed that the percentage of anthraquinone glycosides as well as the toxic effect on the shrimp increased during the vegetation period.

scholarly journals KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

2014 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Ross Nurul Rohmah ◽  
Nuniek Ina Ratnaningtyas ◽  
Ari Asnani
Keyword(s):  
Toxic Effect ◽  
Fruiting Body ◽  
Brine Shrimp ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
One Stage ◽  
Body Extract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

Determination of anthraquinone glycosides in Rhei rhizome, Polygoni multiflori radix, and Cassia torae semen

2020 ◽  
Vol 15 (4) ◽  
Author(s):  
H.-M. Chiang ◽  
H.-T. Tsao ◽  
P.-D.L. Chao ◽  
Y.-C. Hou ◽  
K.-C. Wen
Keyword(s):  
Anthraquinone Glycosides

scholarly journals Toxicity assessment of residual biomass of microalgae Chlorella sorokiniana

2019 ◽  
Vol 59 (7) ◽  
pp. 92-98
Author(s):  
Yulia A. Smyatskaya ◽  
Keyword(s):  
Heat Treatment ◽  
Toxic Effect ◽  
Daphnia Magna ◽  
Chlorella Vulgaris ◽  
Test Object ◽  
Experimental Studies ◽  
Chlorella Sorokiniana ◽  
Lipid Complex ◽  
Residual Biomass

In this paper, the determination of the toxicity of residual biomass of microalgae Chlorella sorokiniana by the method of biotesting with use of the test – object Daphnia magna Straus and algae Chlorella vulgaris Beijer. Determination of the toxic effect is necessary for planning the possibility of further use of waste, as a sorption material for wastewater treatment, as a co-substrate for fermentation of organic waste and in the production of biogas. The residual biomass of Chlorella sorokiniana microalgae is formed after the extraction of valuable components (lipid complex, pigments, proteins, etc.). The extraction of the lipid complex uses organic solvents (hexane, ethyl alcohol), the remains of which can remain in the residual biomass. Determined the acute toxicity of residual biomass of microalgae by water extraction with the help of test-object Daphnia magna Straus and the test object microalgae Chlorella vulgaris Beijer according to standard methods. According to the study waste source of residual biomass to treat the IV class of hazard, ЛКР50-48 = 11.7; БКР10-48 = 35.48. To reduce the toxic effect is proposed to conduct heat treatment. Heat treatment was carried out in a muffle furnace at a temperature of 105 oС for 1 hour. Experimental studies using Daphnia magna Straus crustaceans showed that the value of A is 3%, which indicates the absence of toxicity of residual biomass. When using microalgae toxicity criterion was also not exceeded in any dilution of water, including its original undiluted version. It is established that after heat treatment the residual biomass of microalgae becomes non-toxic and has a V hazard class. The results indicate the possibility of re-use of residual biomass.

scholarly journals Concentrations of Anthraquinone Glycosides of Rumex crispus during Different Vegetation Stages

1994 ◽  
Vol 49 (7-8) ◽  
pp. 404-406
Author(s):  
L. Ömür Demirezer
Keyword(s):  
Thin Layer ◽  
The Other ◽  
Rumex Crispus ◽  
Anthraquinone Glycosides

The anthraquinone glycoside contents of various parts of Rumex crispus L. (Polygonaceae) in different vegetation stages were investigated by thin layer chromatographic and spectro-photometric methods. The data showed that the percentage of anthraquinone glycoside in all parts of plant increased at each stage. Anthraquinone glycoside content was increased in leaf, stem, fruit and root from 0.05 to 0.40%, from 0.03 to 0.46%, from 0.08 to 0.34%, and from 0.35 to 0.91% respectively. From the roots of R. crispus, emodin-8-glucoside, RGA (isolated in our laboratory, its structure was not elucidated), traceable amount of glucofrangulin B and an unknown glycoside (Rf = 0.28 in ethyl acetate:m ethanol:water/100:20:10) was detected in which the concentration was increased from May to August. The other parts of plant contained only emodin-8-glucoside

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