scholarly journals KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST)

2014 ◽  
Vol 1 (1) ◽  
pp. 32
Author(s):  
Ross Nurul Rohmah ◽  
Nuniek Ina Ratnaningtyas ◽  
Ari Asnani
Keyword(s):  
Toxic Effect ◽  
Fruiting Body ◽  
Brine Shrimp ◽  
Ganoderma Lucidum ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
One Stage ◽  
Body Extract

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

scholarly journals Analisis Fitokimia Dan Toksisitas Dari Ekstrak Daun Tumbuhan Mempening (Lithocarpus bancanus (Scheff.) Rehd) Menggunakan Metode BSLT (Brine Shrimp Lethality Test)

2019 ◽  
Vol 2 (2) ◽  
pp. 29-33
Author(s):  
Retno Puji Lestari ◽  
Rudi Hendra Sy ◽  
Hilwan Yuda Teruna
Keyword(s):  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Brine Shrimp Lethality ◽  
The Family ◽  
Tribal Region

Mempening (Lithocarpus bancanus (Scheff.) Rehd) is a species belongs to the family Fagaceae which is found growing around the Talang Mamak tribal region in Kelayang District, Indragiri Hulu Regency, Riau Province. The purpose of this study was to determine the toxicity of leaf extract of L. bancanus (Scheff.) Rehd against Artemia salina Leach larvae using the Brine Shrimp Lethality Test (BSLT) method and its phytochemical analysis. The results of the toxicity was determined against n-hexane, dichloromethane, ethyl acetate, methanol, and ethanol extract and they showed LC50 values ​​of 3.15 ppm; 41163.26 ppm; 5817.09 ppm; 817, 27 ppm; 9019.70 ppm. Phytochemical analysis of the leaves of L. bancanus (Scheff.) Rehd plant showed a class of flavonoids, terpenoids, phenolics and saponins.

scholarly journals Uji Toksisitas Ekstrak Daun Benalu Langsat (Dendrophthoe petandra (L) Miq) dengan Metode Brine Shrimp Lethality Test (BSLT) LC50

Jurnal MIPA ◽  
2015 ◽  
Vol 4 (2) ◽  
pp. 157
Author(s):  
Jhon Smith Wongkar ◽  
Max R. J. Runtuwene ◽  
Jemmy Abidjulu
Keyword(s):  
Toxic Effect ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
North Sulawesi ◽  
Experimental Organism

Telah dilakukan penelitian tentang toksisitas daun benalu langsat (Dendrophthoe petandra) dengan metode Brine Shrimp Letality Test (BSLT). Metode ini menggunakan etanol sebagai pelarut untuk mengekstrak daun benalu langsat dan udang Artemia salina sebagai organisme uji serta dilakukan pada suhu 25-30 0C. Sampel benalu langsat yang di uji didapatkan dari Desa Kali Selatan Kecamatan Pineleng Kabupaten Minahasa Sulawesi utara. Penentuan toksisitas dari ekstrak etanol daun benalu langsat menggunakan konsentrasi 500, 100, 20, 10, dan 1 ppm. Hasil penelitian menunjukkan bahwa semakin besar konsentrasi yang diberikan maka sifat toksisitas ekstrak daun benalu langsat juga akan semakin besar dengan nilai LC50 sebesar 0,561.A research on toxicity of benalu langsat (Dendrophthoe petandra) leaf by using Brine Shrimp Letality Test (BSLT) method has been done. This method using ethanol as solvent to extract benalu langsat leaf and shrimp Artemia salina as experimental organism at 25-30 oC. Benalu langsat leaf was obtained from village South Kali district Pineleng, Minahasa North Sulawesi. Toxicity examination used 500, 100, 20, 10, dan 1 ppm ethanol extract of benalu langsat leaf. The results showed that the higher the concentration of the extract, the higher the toxic effect shown with LC50 value of 0.561.

scholarly journals Toxicity Test of Ethanol Extract Ant Plant Local Aceh (Mymercodia sp) Method of BSLTLarvae Shrimp Artemia salina Leach

2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Frengki F ◽  
Roslizawaty R ◽  
Desi Pertiwi
Keyword(s):  
Crude Extract ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Rotary Evaporator

Toxicity test and phytochemical screening of ant plant ethanol extract were done. The toxicity test was done by employing the Brine Shrimp Lethality Test (BSLT) method. Sample of ant plant taken from the Aceh Besar forest have been extracted with ethanol. The sample was thenconcentrated using a rotary evaporator until a crude extract was obtained. The extract was tested by toxicity to larva Artemia salina L. Thetoxicity test for larvae Artemia salina Leach have been done by employing the Brine Shrimp Lethality Test (BSLT) method at four concentrationlevels, i.e: 250 ppm, 125 ppm, 50 ppm, and 25 ppm. The result of the research showed that ethanol extract of ant plant Aceh had acute toxicityby LC value 61.11 ppm. The extract had identified were fenolik, saponin, steroid and terpenoid.____________________________________________________________________________________________________________________Key words: rine Shrimp Lethality Test, LC5050, Ant Plant, Aceh, Bphytochemical Screening 

scholarly journals ANALISIS SENYAWA METABOLIT SEKUNDER DAN UJI TOKSISITAS EKSTRAK ETANOL BATANG TANAMAN PATAH TULANG (Euphorbia tirucalli L.) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2014 ◽  
Vol 14 (2) ◽  
pp. 106 ◽  
Author(s):  
Grace S. Baud ◽  
Meiske S. Sangi ◽  
Harry S.J. Koleangan
Keyword(s):  
Secondary Metabolite ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
Euphorbia Tirucalli ◽  
Ethanol Extracts

ABSTRAK Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman Patah tulang (Euphorbia tirucalli L.). Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia yaitu senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak segar dan kering batang tanaman Patah tulang. Penentuan toksisitas ekstrak etanol batang tanaman Patah tulang menggunakan metode Brine Shrimp Lethality Test (BSLT). Uji toksisitas digunakan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh positif flavonoid, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan metode probit menggunakan perangkat lunak SPSS 20.0 (untuk sistem operasi Windows). Hasil uji toksisitas ekstrak etanol batang Patah tulang menunjukkan bahwa ekstrak segar maupun kering bersifat sitotoksik dengan nilai LC50 (7,994ppm) untuk ekstrak etanol batang segar  dan LC50 (9,940ppm) untuk ekstrak etanol batang kering. Kata kunci : Euphorbia tirucalli L., Senyawa metabolit sekunder, Toksisitas, Artemia salina Leach, LC50.   ANALYSIS OF SECONDARY METABOLITE COMPOUNDS AND TOXICITY TEST OF  STEM  PLANT ETHANOL EXTRACTS OF  PATAH TULANG (Euphorbia tirucalli L.) BY BRINE SHRIMP LETHALITY TEST’S METHOD (BSLT) ABSTRACT A research on the analysis of secondary metabolite compounds contained in the stem of  patah tulang (Euphorbia tirucalli L.) and its toxicity had been done. The analysis was performed through phytochemical screening of alkaloids, flavonoids, triterpenoids, steroids, saponins and tannins on extracts of fresh and dried stem of the plant. Toxicity of ethanol extract of the stem was determined using Brine Shrimp Lethality Test (BSLT) method with Artemia salina Leach as bioindicator. Phytochemical screening showed positive results for flavonoids, tannins, and steroids and negative one for alkaloids, saponins, and triterpenoids. Toxicity data were obtained from Lethality Concentration 50 (LC50) calculation trhrough probit analysis using SPSS 20.0 software (for Windows operating systems). Toxicity test showed that ethanol extracts of the fresh and dried stem were cytotoxic with LC50 value of 7.994 ppm and 9.940 ppm, respectively. Keywords: Euphorbia tirucalli L., Secondary metabolite compounds, Toxicity, Artemia salina Leach, LC50.

scholarly journals A Cytotoxic Activity of Tahongai (Kleinhovia hospita Linn.) Leaves Extracts Using Brine Shrimp Lethality Test

2019 ◽  
Vol 4 (3) ◽  
pp. 60
Author(s):  
Indah Solihah ◽  
Herlina Herlina ◽  
Riana Sari Puspita Rasyid ◽  
Tri Suciati ◽  
Khairunnisa Khairunnisa
Keyword(s):  
Statistical Analysis ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Brine Shrimp Lethality ◽  
Ethanol Extracts

Tahongai (Kleinhovia hospita Linn.) leaves had been known contain alkaloids, flavonoids, saponins, steroids, and tannins. At Komering, South Sumatera tribe, tahongai leaves had been known to treat tumor, cancer, polyps, acne, and dysmenorrhea. The study of cytotoxic activity of tahongai bark and stem were done. This study aims to determine the cytotoxic activity of tahongai leaves extracts using BSLT method. Tahongai leaves were extracted using gradual maceration with n-hexane, ethyl acetate, and ethanol 96%. Each extract was tested cytotoxic activity towards Artemia salina L. larvae. The yield of n-hexane, ethyl acetate, and ethanol extracts are 2,686%, 7,033%, and 7,933% respectively. Ethanol extract of tahongai leaves had the best cytotoxic activity with lethality value 76,667% at 500ppm. Statistical analysis with two way ANOVA showed extract and concentration had a significant (p<0,05) effect on larvae lethality percentage.

scholarly journals UJI TOKSISITAS EKSTRAK ETANOL DAUN JAMBU BIJI AUSTRALIA (Psidium guajava L) DENGAN METODE BSLT (Brine Shrimp Lethality Test)

2021 ◽  
Vol 9 (1) ◽  
pp. 10-17
Author(s):  
Nofita Nofita ◽  
◽  
Ade Maria Ulfa ◽  
Miera Delima ◽  
◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Ultrasonic Method ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Test Method ◽  
Toxicity Tests ◽  
Brine Shrimp Lethality ◽  
Guava Leaves

ABSTRACTGuava is one of the plants that can traditionally be used for the treatment of diseases. Many kinds of guava, one of which is the Australian guava has the characteristics of roots, stems, leaves, dark red fruit. This study aims to determine the toxicity of the ethanol extract of Australian guava leaves (Psidium guajava L) using the BSLT (Brine Shrimp Lethality Test) method and determine the chemical content of Australian guava leaves (Psidium guajava L). The extract was made by the ultrasonic method using 96% ethanol solvent. Toxicity tests were carried out using 48-hour-old Artemia salina Leach shrimp larvae. The toxic effect of the extract was identified by the percentage of shrimp larvae mortality using probit analysis (LC50). From the research results, phytochemical content includes tannins, flavonoids, alkaloids, terpenoids and saponins, and flavonoid compounds have the highest content compared to the others. Research shows that the ethanol extract of Australian guava leaves is of a moderate category (LC50 441,977 ppm).Keywords :Australia guava leaves, BSLT, Artemia salina L, Ultrasonic

scholarly journals Analisis Senyawa Metabolit Sekunder dan Uji Toksisitas Ekstrak Batang Bawang Laut (Proiphys amboinensis (L.) Herb.)

Jurnal MIPA ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 144
Author(s):  
Chendy Christy Dapas ◽  
Harry S. J. Koleangan ◽  
Meiske S. Sangi
Keyword(s):  
Secondary Metabolites ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Phytochemical Screening ◽  
Steroid Saponin ◽  
Brine Shrimp Lethality ◽  
Probit Method ◽  
Dried Extract

Telah dilakukan penelitian mengenai analisis senyawa metabolit sekunder dan uji toksisitas pada batang tanaman bawang laut (Proiphys amboinensis (L.) Herb.) Analisis senyawa metabolit sekunder dilakukan dengan skrining fitokimia untuk senyawa alkaloid, flavonoid, triterpenoid, steroid, saponin dan tanin pada ekstrak batang tanaman bawang laut segar dan kering dan selanjutnya dianalisis menggunakan spektrofotometer UV-VIS. Penentuan toksisitas ekstrak batang tanaman bawang laut menggunakan metode Brine Shrimp Lethality Test (BSLT) dengan hewan uji Artemia salina Leach sebagai bioindikator. Pada skrining fitokimia diperoleh flavonoid dan  steroid positif sedangkan alkaloid, saponin triterpenoid dan tanin negatif. Analisis spektofotometer UV-VIS ekstrak etanol menunjukkan puncak serapan pada 304,40 dan  284,50 nm. Data pengujian toksisitas diperoleh dari analisis Lethality Concentration 50 (LC50) yang dilakukan dengan  metode probit menggunakan perangkat lunak SPSS 20. Hasil uji toksisitas ekstrak batang tanaman bawang laut menunjukkan bahwa  ekstrak segar maupun kering bersifat sangat toksik dengan nilai LC50 kurang dari 30 ppm, yaitu: 9,978 ppm untuk batang kering dan 3,980 ppm untuk batang segar.A research has been done on the analysis of secondary metabolites and toxicity examination on the stems of the sea onion (Proiphys amboinensis (L.) Herb.). Analysis of secondary metabolites carried out with the phytochemical screening for alkaloid compounds, flavonoids, triterpenoids, steroids, saponins, and tannins in extracts of fresh and dried stems of the sea onion, then analyzed using UV-VIS spectrophotometer. The toxicity determination of stems extract of the sea onion plant was done by using Brine Shrimp Lethality Test (BSLT) with Artemia salina Leach as bioindicator. Phytochemical screening showed positive test for flavonoids and steroids while alkaloids, saponins, triterpenoids, and tannins were negative. UV-VIS  spectrophotometer analysis on ethanol extract showed peaks at 304,40 and 284,50 nm. Data of the toxicity examination was obtained from the analysis of Lethality Concentration 50 (LC50) which were performed with the probit method using SPSS 20. Results of toxicity examination of sea onion stems extract showed that fresh or dried extract are very toxic with LC50 values ​​of less than 30 ppm, namely: 9,978 ppm for fresh stems and 3,980 ppm for dried stems.

scholarly journals ANALISIS FITOKIMIA DAN PENENTUAN NILAI LC50 EKSTRAK METANOL DAUN LIWAS

2014 ◽  
Vol 14 (2) ◽  
pp. 125
Author(s):  
Gerry Sumihe ◽  
Max R. J. Runtuwene ◽  
Johnly A. Rorong
Keyword(s):  
Secondary Metabolites ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Artemia Salina ◽  
Phytochemical Analysis ◽  
Test Results ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

ABSTRAK Penentuan nilai LC50 dari ekstrak metanol daun liwas menggunakan metode Brine Shrimp Lethality Test (BSLT) terhadap hewan indikator udang Artemia salina menunjukkan nilai LC50 dari  A. salina bersifat sangat toksik.Nilai LC50 yang didapatkan sebesar 15,696 mg/L. Nilai LC50 ditentukan dengan menggunakan metode SPSS 20.0.Ekstrak metanol daun liwas mengandung kandungan senyawa metabolit sekunder seperti flavonoid, tanin dan saponin sebagai hasil uji fitokimia. Kata kunci: BSLT, Daun Liwas, Metabolit Sekunder,LC50.   PHYTOCHEMICAL ANALYSIS AND DETERMINING LC50 VALUE OF LIWAS LEAVE METHANOL EXTRACT ABSTRACT Determination of LC50 of methanol extract of leaves liwas using Brine Shrimp Lethality Test (BSLT) against animal indicator shrimp Artemia salina showed LC50 values of  A. salina is highly toxic. LC50 values were obtained at 15.696 mg / L. LC50 values determined using SPSS 20.0. Liwas leaf methanol extract contains compounds secondary metabolites such as flavonoids, tannins and saponins as phytochemical test results. Keywords: BSLT, Leaves Liwas, Secondary Metabolites, LC50.

UJI TOKSISITAS AKUT EKSTRAK ETANOL AKAR KB (Coptosapelta tomentosa Valeton ex K.Heyne) DENGAN METODE Brine Shrimp Lethality Test (BSLT)

2017 ◽  
Vol 2 (2) ◽  
pp. 161
Author(s):  
Risa Supriningrum ◽  
Sapri Sapri ◽  
Vici Ali Pranamala
Keyword(s):  
Acute Toxicity ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Analysis ◽  
Test Results ◽  
Brine Shrimp Lethality ◽  
Lc50 Value

The roots of KB plants (Captosapelta tomentisa) is used empiricallyby  Dayak Kenyah community as a drug leukorrhea. Leukorrhea is one of the early symptoms of cervical cancer. Early methods to determine the LC50 value or toxicity of anticancer KB roots have not been reported. The aim of research is to determine the value of the toxicity of KB root. Research was conducted by an experimental study. Research stages include sample processing, extraction and acute toxicity test by Brine Shrimp Lethality Test (BSLT). BSLT testing to determine LC50 values with a concentration of 250 ppm, 300 ppm, 400 ppm, 450 ppm and a negative control. Analysis of the data used are probit analysis method. The results showed  the presence of flavonoids, alkaloids and saponins in of the ethanol extract of KB root . The test results obtained acute toxicity LC50 value is 299.226 mg / ml, so that the ethanol extract of roots KB potentially toxic to the larvae of Artemia salina Leach.

scholarly journals UJI Brine Shrimp Lethality Test (BSLT) UMBI BAWANG TIWAI (Eleutherine bulbosa (Mill.) Urb) DAN UJI TOKSISITAS AKUT FRAKSI AKTIF

2019 ◽  
Vol 1 (1) ◽  
pp. 1-10
Author(s):  
Dwi Lestari ◽  
Rudi Kartika ◽  
Eva Marliana
Keyword(s):  
Brine Shrimp ◽  
Ethanol Extract ◽  
Artemia Salina ◽  
Chloroform Fraction ◽  
Brine Shrimp Lethality ◽  
Method Of Calculation ◽  
Water Fraction ◽  
Randomized Design ◽  
Ld50 Value ◽  
Selection Of

Eleutherine bulbosa (Mill.) Urb come from the Iridaceae family, this species contains secondary metabolites in the form of flavonoids and quinones. This research method includes Brine Shrimp Lethality Test (BSLT) toxicity using Artemia salina Leach larvae to determine the LC50 value. This study used experimental methods pharmacologically using a completely randomized design in the same direction pattern in the selection of test animals, 25 animals used were white mice divided into 5 groups with 5 mice per group, with 4 hours observed to find out toxic symptoms and continued observation every 24 hours to see death. Testing of BSLT results showed that ethanol extract had LC50 toxicity = 66.68 ppm (very toxic category), n-hexane fraction had LC50 toxicity = 47.64 ppm (very toxic category), chloroform fraction had LC50 toxicity = 295.1 ppm ( toxic category), and the water fraction has a toxicity of LC50 = 194.54 ppm (very toxic category). The chloroform fraction is an active fraction. Acute toxicity test LD50 value according to the calculation method of Miller Tainter Probit (187,499 mg/KgBB), the method of calculation of Thompson Weil (182,810 mg/KgBB), how to calculate Indonesian Pharmacopoeia (187,068 mg/KgBB). Based on the three calculations, the LD50 value is in the medium category (50-500 mg/kgBB).

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