A New Hepatoprotective Flavone Glycoside from the Flowers of Onopordum alexandrinum Growing in Egypt

2011 ◽  
Vol 66 (5-6) ◽  
pp. 251-259 ◽  
Author(s):  
Maha M. Salama ◽  
Shahira M. Ezzat ◽  
Amany A. Sleem
Keyword(s):  
Free Radical ◽  
Cell Damage ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Flavone Glycoside ◽  
H Nmr ◽  
C Nmr

A bioactivity-guided fractionation of the ethyl acetate fraction of the flowers of Onopordum alexandrinum L. (Asteraceae) yielded a new flavonoidal glycoside designated as acacetin-7-O-galacturonide (9), alongside with nine known flavonoids; 6-methoxy-apigenin (hispidulin) (1), acacetin (2), apigenin (3), luteolin (4), kaempferol (5), eriodictyol (6), apigenin- 7-O-glucoside (7), luteolin-7-O-glucoside (8), and kaempferol-3-O-rutinoside (10). The compounds were assayed for their hepatoprotective activity against CCl4-induced hepatic cell damage in rats and free radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Compounds 4, 6, 9, and 10 have not been previously reported from flowers of O. alexandrinum L., and this is the first report of acacetin-7-O-galacturonide (9) in nature which has also shown significant hepatoprotective and free radical scavenging effects. The isolated compounds were identified using different spectroscopic methods (UV, 1H NMR, 13C NMR, HMQC, HMBC, and COSY).

IN VITRO ANTIOXIDANT AND IN VIVO HEPATOPROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF ZIZIPHUS RUGOSA LAM. LEAVES

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (07) ◽  
pp. 69-75
Author(s):  
S Parashar ◽  
V. Uplanchiwar ◽  
R. K. Gautam ◽  
S. Goyal ◽  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ic50 Value

Ziziphus rugosa Lam. belongs to the family Rhamnaceae and is found chiefly in deciduous and semi evergreen forest of Western Ghats. The present research was undertaken to establish in vitro antioxidant and in vivo hepatoprotective activity of ethanolic extract of Z.rugosa Lam. leaves. The powdered leaves of Z. rugosa were extracted with ethanol and preliminary phytochemical screening was performed for the presence of various phytoconstituents. DPPH assay and β-glucuronidase inhibition assay were selected for the free radical scavenging activity. For the assessment of hepatoprotective activity, alcohol and CCl4 induced hepatotoxicity model were used. The phytochemical analysis of ethanolic extract showed the presence of alkaloids, saponins and flavonoids. The extract exhibited concentration dependent radical scavenging activity with an IC50 value of 61.88 μg/ml and β –glucoronidase inhibition activity with an IC50 value of 70.61 μg/ml. It was speculated that the Z. rugosa Lam. ethanolic extract shows dosedependent hepatoprotective activity which is equivalent with the standard drug Silymarin. The inhibition of free radicals or free radical scavenging activity is significant in the protection against CCl4 and alcohol induced hepatopathy. Hence, it is likely that the antioxidant activity of ethanolic extract of Z. rugosa Lam. might contribute to the hepatoprotective action.

scholarly journals Isolasi dan Aktivitas Antioksidan Fraksi dari Ekstrak Tongkol Jagung

2017 ◽  
Vol 37 (2) ◽  
pp. 139
Author(s):  
Edi Suryanto ◽  
Lidya Irma Momuat
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Corn Cob ◽  
Total Antioxidant

The corn cob is one of the food waste-material having phytochemical component that of health benefit. The objectives of the research were to isolate phenolic fractions of the corn cob extract and to determine antioxidant and antiphotooxidant activities. The corn cob was extracted with reflux method using ethanol 80% for 2 hours. After that the extracts were filtered and the filtrates were combined and concentrated on rotary evaporator. This crude ethanolic extract was suspended in water and extracted with petroleum ether, ethyl acetate, n-butanol and water, respectively. The best fractions of partition fractionated with colomn chromatography using silica gel 60 and n-hexane: ethyl acetate (4:6). Corncob extract and fraction were evaluated for total phenolic content free radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl radical assay) and total antioxidant capacity (ferric reducing ability of plasma assay). The results of total phenolic content showed that ethyl acetate fraction (163.57 μg/mL) has higher phenolic content than butanol fraction (83.30 μg/mL), ethanol extract (81.53 μg/mL), water fraction (23.71 μg/mL) and petroleum ether fraction (23.57 μg/mL). The results also showed that ethyl acetate fraction has highest free radical scavenging activity and total antioxidant capacity than butanol fraction, ethanol extract, water fraction and petroleum ether fraction. The free radical scavenging activity of fractions II and III exhibited strongest activity than fraction V, I, IV and VI. The results also showed that fraction II has highest total antioxidant capacity than I, III, IV, VI, and V. The results suggest that the ethyl acetate fraction and fraction II contains phenolic compounds which possess antioxidant activities. ABSTRAKTongkol jagung merupakan salah satu limbah tanaman pangan yang mempunyai kandungan fitokimia yang bermanfaat untuk kesehatan. Penelitian ini bertujuan untuk mengisolasi fraksi fenolik dari ekstrak tongkol jagung dan menentukan aktivitas antioksidan. Tongkol jagung diekstraksi dengan cara refluk menggunakan etanol 80% selama 2 jam. Setelah itu disaring dan filtratnya diuapkan dengan rotary evaporator. Ekstrak etanol disuspensikan dalam air dan diekstraksi berturut turut dengan petroleum eter, etil asetat, butanol, dan air. Fraksi pelarut terbaik difraksinasi dengan kromatografi kolom menggunakan silika gel 60 dan eluen n-heksana : etil asetat (4:6). Ekstrak tongkol jagung dan fraksi dievaluasi kandungan total fenolik, aktivitas penangkal radikal bebas (uji 1,1-difenil-2-pikrilhidrazil) dan kapasitas total antioksidan (uji ferric reducing ability of plasma). Hasil uji kandungan total fenolik menunjukkan bahwa fraksi etil asetat (163,57 μg/mL) memiliki kandungan total fenolik yang paling tinggi daripada fraksi butanol (83,30 μg/mL), ekstrak etanol (81,53 μg/mL), fraksi air (23,71 μg/mL) dan fraksi petroleum eter (23,57 μg/mL). Hasil ini juga menunjukkan bahwa fraksi etil asetat mempunyai aktivitas penangkal radikal dan kapasitas total antioksidan paling tinggi daripada fraksi butanol, ekstrak etanol, fraksi air, dan fraksi petroleum eter. Aktivitas penangkal radikal bebas fraksi II dan III memperlihatkan paling kuat daripada fraksi V, I, IV dan VI. Hasil penelitian ini menyarankan bahwa fraksi etil asetat dan fraksi II mengandung senyawa fenolik yang mempunyai aktivitas antioksidan kuat.

Antioxidative Activities of the Hydrolyzed Sasanquasaponins from the Defatted Seeds of Camellia oleifera Abel

2013 ◽  
Vol 781-784 ◽  
pp. 1068-1071
Author(s):  
Yong Ye ◽  
Hai Ting Xing ◽  
Xue Lan Chen
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Intragastric Administration ◽  
Hydrophobic Property ◽  
H Nmr ◽  
Antioxidative Effects

The purpose of this research is to isolate sasanquasaponin and its hydrolyzed products from the defatted seeds of C. oleifera and evaluate their antioxidative effects in vitro and in vivo. Their structures were analyzed by UV, MS, 1H NMR and 13C NMR spectra. Free radical scavenging activities in vitro were determined by DPPH and ABTS; antioxidative activities in vivo were assessed by MDA, SOD and GSH-Px levels in serum and brain tissue of mice after intragastric administration of the products at 50, 200 mg/kg/d for 30 days. Acid hydrolyzed product and sapogenin had stronger antioxidative activities than the sasanquasaponin. They eliminated DPPH and ABTS radicals, significantly (p<0.01) improved SOD and GSH-Px activity in blood, and decreased MDA content in brain, but sasanquasaponin and alkaline hydrolyzed product had no significant change (p>0.05). On the basis of molecular structure in sasanquasaponin, hydroxyl and tigloyl groups are helpful to free radical scavenging activity; smaller molecules and hydrophobic property are beneficial to their absorption and antioxidative effects in vivo. Acid hydrolyzed product and sapogenin are more valuable to protect body against damage from free radicals.

Synthesis of Oil-Soluble Aliphatic Acylated Epicatechin and their Free Radical Scavenging Activity

2013 ◽  
Vol 781-784 ◽  
pp. 1424-1429
Author(s):  
Zhe Gao ◽  
Yu Lei Pan ◽  
Ai Xia Qin ◽  
Xiao Hu Kang ◽  
Xi Yue Li ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Nmr Spectroscopy ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Tea Polyphenol ◽  
H Nmr ◽  
Better Than

A series of epicatechin derivatives, including 3-O-palmitoyl epicatechin, 3-O-myristoyl epicatechin, 3-O-lauroyl epicatechin, 3-O-decanoyl epicatechin and 3-O-octanoyl epicatechin, were prepared by the acylation method. Structures of these prepared products were characterized by LC/MS and 1H-NMR spectroscopy. Their antioxidant activity were assessed by the methods of DPPH and ABTS free radical scavenging. The results showed that aliphatic acyl epicatechin derivatives with varying aliphatic groups were synthesized by this method. The antioxidant activity of aliphatic acyl epicatechin derivatives were better than the oil-soluble tea polyphenol and BHT, equal to TBHQ.

Antioxidant Metabolites from Limonium brasiliense (Boiss.) Kuntze

2004 ◽  
Vol 59 (7-8) ◽  
pp. 477-480 ◽  
Author(s):  
Ana P. Murray ◽  
Silvana Rodriguez ◽  
María A. Frontera ◽  
María A. Tomas ◽  
María C. Mulet
Keyword(s):  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Methods ◽  
H Nmr ◽  
Antioxidant Metabolites ◽  
First Time ◽  
C Nmr

Abstract A free radical scavenging activity guided fractionation of the polar extract from roots of Limonium brasiliense (Plumbaginaceae) led to the isolation of five active compounds including: myricetin 3-O-α-rhamnopyranoside (1), (-)-epigallocatechin 3-O-gallate (2), (-)-epigallocatechin (3), (+)-gallocatechin (4) and gallic acid (5). These and other chemical constituents are reported for the first time for this species. The characterization of these compounds was achieved by spectroscopic methods (1H NMR, 13C NMR and UV).

scholarly journals CHARACTERIZATION, IN VITRO ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF FUNGAL ENDOPHYTIC EXTRACTS OF ANDROGRAPHIS PANICULATA LEAVES IN CCL4 INDUCED HEPATOTOXICITY

2019 ◽  
Vol 11 (1) ◽  
pp. 44 ◽  
Author(s):  
Smita K. Puri ◽  
Prasanna V. Habbu ◽  
Preeti V. Kulkarni ◽  
Venkatrao H. Kulkarni
Keyword(s):  
Free Radical ◽  
Endophytic Fungi ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Andrographis Paniculata ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Objective: To isolate and characterize the endophytic fungi from the leaves of Andrographis paniculata for free radical scavenging antioxidant and hepatoprotective activity against CCl4 induced hepatotoxicityMethods: Two fungal endophytes, APLF-3 (Andrographis paniculata leaf fungi-3) and APLF-4 (Andrographis paniculata leaf fungi-4) were isolated from leaves of Andrographis paniculata to get chloroform (A3C, A4C), ethyl acetate (A3EA, A4EA) and n butanol (A3nB, A4nB) extracts. rDNA sequencing by PCR technique was carried out for identification of APLF-3 and APLF-4. All the APLF-3 and APLF-4 extracts were assayed for in vitro free radical scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and reducing power. Then, A4EA and A4nB were screened for hepatoprotective activity against CCl4 induced hepatotoxicity at 50 mg/kg and100 mg/kg doses.Results: The endophytic fungi, APLF-3 and APLF-4, were identified as Phyllosticta sp. ZLY-2010 isolate M13 and Aspergillus tubingensis strain Cs/7/2 respectively based on their morphological and molecular characterization. A4EA and A4nB showed significant in vitro free radical scavenging activity as compared to other extracts. A4EA and A4nB (50 mg/kg and100 mg/kg) reversed the increased serum biochemical parameters as compared to CCl4 treated group (p<0.001). A4EA and A4nB (100 mg/kg p. o) also restored the LPO, SOD and CAT levels.Conclusion: These findings suggested that the extracts (A4EA and A4nB) obtained from endophytic fungi APLF-4 contributed towards hepatoprotective activity.

scholarly journals Free Radical Scavenging Activity of Aerial Parts of Artemisia pallens in Mediating Hepatoprotective Activity of RIF+INH Intoxicated Rats

2019 ◽  
Vol 14 ◽  
pp. 1-15
Author(s):  
Praveen Kumar Ashok ◽  
Poonam Rishishwar ◽  
Kumud Upadhyaya
Keyword(s):  
Nitric Oxide ◽  
Free Radical ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dry Extract ◽  
Artemisia Pallens

To investigate the free radical scavenging & hepatoprotective activity of phenolic rich fraction of Artemisia pallens on RIF+INH induced oxidative stress in Sprague dawley rats. Free radical scavenging and hepatoprotective activity was evaluated by using DPPH, Nitric oxide, Superoxide radical and Hydroxyl radical assay models and induced RIF+INH intoxicated rats. The total phenolic content was found to be 312.60 μg gallic acid equivalents (GAE)/gm of dry extract. The total flavonoid content was found to be 322.20 μg rutin equivalents (RUE)/gm of dry extract. In the current study, free radical scavenging activity was evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide, superoxide radical and hydroxyl radical scavenging activity were found to be (42.25 ± 0.95) and (09.16 ± 1.62) μg/ml, (101.62 ± 1.64) and (32.41 ± 1.24) μg/ml, (72.62 ± 1.86) and (10.28 ± 1.96) μg/ml, (33.82 ± 1.12) and (12.82 ± 1.86) μg/ml, respectively. There was also a dose dependent increase in reductive ability of Artemisia pallens extract with increase in concentration and were further investigated in invivo hepatoprotective activity experiment against toxicity induced by RIF+INH. The free radical scavenging and hepatoprotective activity may be attributed to the presence of phenolic compounds and histology of the liver section of the animals treated with the extracts showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration, which further evidenced the hepatoprotective activity of Artemisia pallens.

scholarly journals Free Radical Scavenging Activity of Ethanolic Leaves Extract and Its Different Solvent Fractions of Piper betle L. In Vitro

2011 ◽  
Vol 2 (1) ◽  
pp. 141
Author(s):  
Chandra Risdian ◽  
Wahyu Widowati ◽  
Tjandrawati Mozef ◽  
Teresa Liliana Wargasetia ◽  
Khie Khiong
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Piper Betle ◽  
Scavenging Activity

Various diseases including cancer and cardiovascular diseases are induced by free radicals via lipid peroxidation, protein peroxidation, DNA damage, and cellular degeneration in the cells. Piper betle L., commonly called betel, belongs to the Piperaceae family. It originated from South and South East Asia, and has been used as a traditional medicine by people there due to its medicinal properties. In the present study, free radical scavenging activity of ethanolic leaves extract of Piper betle L. together with its different solvent fractions (hexane, ethyl acetate, butanol, and water) were evaluated by DPPH free radical scavenging assay. The ethanolic extract and the ethyl acetate fraction had shown very significant DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity compared to the other fractions. In DPPH free radical scavenging assay, the IC50 value of the ethanolic extract and the ethyl acetate fraction were found to be 17.43µg/mL and 11.53 µg/mL, respectively. The free radical scavenging activity was assumed due to the total phenolic content. The total phenolic content was found to be highest in the ethanol extract (277.68 μg EGCG/mg) and the ethyl acetate fraction (559.38μg EGCG/mg). The results concluded that the ethanolic leaves extract of Piper betle L. and its ethyl acetate fraction have a considerably free radical scavenging activity.Keywords: Piper betle L., Piperaceae, free radical scavenging, DPPH

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