scholarly journals Selective and nonselective stimulation of central cholinergic and dopaminergic development in vitro by nerve growth factor, basic fibroblast growth factor, epidermal growth factor, insulin and the insulin-like growth factors I and II

1990 ◽  
Vol 10 (2) ◽  
pp. 558-570 ◽  
Author(s):  
B Knusel ◽  
PP Michel ◽  
JS Schwaber ◽  
F Hefti
Keyword(s):  
Growth Factors ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Fibroblast Growth ◽  
Epidermal Growth ◽  
Development In Vitro ◽  
Stimulation Of ◽  
Insulin Like Growth Factors

Synergistic effect of basic fibroblast growth factor (bFGF) and epidermal growth factor on derivation of camel (Camelus dromedarius) trophoblast stem cells

Zygote ◽  
2019 ◽  
Vol 27 (4) ◽  
pp. 255-258
Author(s):  
Faisal A. Alzahrani
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Control Group ◽  
Fibroblast Growth ◽  
Trophoblast Stem Cells ◽  
Trophoblast Stem ◽  
Epidermal Growth

SummaryThis study aimed to optimize the derivation of trophectoderm from in vitro-produced camel embryos under feeder-free culture conditions using the basement membrane matrix Matrigel. Trophoblastic vesicles were obtained through mechanical microdissection of in vitro-produced camel (Camelus dromedarius) embryos. Supplementing the culture medium with 10 ng/ml of epidermal growth factor and 10 ng/ml fibroblast growth factor improved the attachment and subsequent outgrowths of cultured trophoblastic vesicles when compared with the control group and the groups supplemented individually with each growth factor. The expression levels of pluripotency genes octamer-binding transcription factor 4 (Oct4), sex determining region Y-box 2 (Sox2), myelocytomatosis proto-oncogene (c-Myc) and anti-apoptotic gene B-cell lymphoma 2 (Bcl2) were increased in trophoblastic vesicles supplemented with both growth factors when compared with the control group. Conversely, both growth factors decreased the expression of apoptotic genes tumour protein p53 (p53) and Bcl-2-associated X protein (Bax). To the best of our knowledge, this may be the first report describing the derivation of trophoblast stem cells from in vitro-produced camel embryos.

Stimulation of luteinizing hormone release by epidermal growth factor is mediated by arachidonic acid metabolism in vitro

1988 ◽  
Vol 117 (4_Suppl) ◽  
pp. S54-S55
Author(s):  
A. PRZYLIPIAK ◽  
L. KIESEL ◽  
T. RABE ◽  
K. HELM ◽  
M. PRZYLIPIAK ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Growth Factor ◽  
Luteinizing Hormone ◽  
Epidermal Growth Factor ◽  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
Hormone Release ◽  
Epidermal Growth ◽  
Stimulation Of
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