scholarly journals Integrated proteomics and histochemical analysis of Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae) in embryogenic suspension culture

2020 ◽  
Vol 63 (2) ◽  
pp. 27-43
Author(s):  
Francis Pereira-Dias ◽  
Neusa Steiner ◽  
Gabriela Cangahaula-Inocente ◽  
Ana Paula Lando ◽  
Marisa Santos ◽  
...  
Keyword(s):  
Cell Growth ◽  
Suspension Culture ◽  
Cell Viability ◽  
Cell Suspension ◽  
Growth Dynamics ◽  
Exponential Phase ◽  
Araucaria Angustifolia ◽  
Fresh Weight ◽  
Embryogenic Cell ◽  
Proembryogenic Masses

t Cell suspension culture is a useful in vitro model-system for both scaling up and conserving the Brazilian conifer Araucaria angustifolia. In the present work, cell suspension of Araucaria was subjected to proteomics, biochemical and histochemical analyses. The results revealed new insights underlying the molecular mechanism of proembryogenic masses transition in cell suspension. Embryogenic cell cultures were cultivated in a basal liquid medium modified in a Steward apparatus (orbital agitator). Cell growth dynamics was evaluated using cell volume after sedimentation, fresh weight, mitotic index, conductivity, pH, and the number of proembryogenic masses (PEMs: I, II, III). Histochemical parameters, cell viability, and cell death analyses were performed to pinpoint growth rates. Proteomics analysis was performed using two-dimensional electrophoresis, and protein identification was carried out by MALDI-TOF-TOF tandem mass spectrometry. Cell growth dynamics showed a predominance of PEM III. Maximum slope of the exponential phase growth in fresh weight occurred at exponential phase after 15 days (optimal cultivation time), after which cell viability and pH decreased, thereby allowing the identification of stressrelated proteins. Several metabolism and growth proteins were abundant, such as: cytoskeletal, WOX1, cytokinin-related, and auxin-related proteins acting on cell wall modification, suspensor cell formation, and PEM I to PEM III transition.

scholarly journals Initiation of coconut cell suspension culture from shoot meristem derived embryogenic calli: A preliminary study

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
U. Bhavyashree ◽  
K. Lakshmi Jayaraj ◽  
K. S. Muralikrishna ◽  
K. K. Sajini ◽  
M. K. Rajesh ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Fluorescent Microscopy ◽  
Cell Aggregation ◽  
Shoot Meristem ◽  
Malt Extract ◽  
Embryogenic Calli ◽  
Embryogenic Cell

<p>An attempt was made to establish highly competent embryogenic cell suspension culture in coconut, a species recalcitrant to in vitro culture. Embryogenic calli were initiated from shoot meristem explants of coconut. Y3 medium supplemented with 2.4-D (4.5 μM) and glutamine (34.2 μM) was found to be the best medium to initiate cell suspension. Growth evaluation was done by packed cell volume (PCV) and it was found that maximum growth volume of 9.9% was reached at 200 days of culture initiation. About 52% of viable cells were detected through fluorescent microscopy. Cell aggregation was noticed in Y3 medium supplemented with glutamine (34.2 μM), malt extract (100mg/l), biotin (40.9 μM) and kinetin (9.3 μM), but further progress could not be achieved. It was also observed that embryogenic calli were not of a friable type, but were associated with densely aggregated cells. Because of its hard nature, we were unsuccessful to obtain high quality cell suspension.</p>

scholarly journals Somatic embryogenesis in Lolium multiflorum suspension culture

2014 ◽  
Vol 65 (1-2) ◽  
pp. 43-45
Author(s):  
Margarita Pavlova ◽  
Elizabeth Kordyum
Keyword(s):  
Somatic Embryogenesis ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Nutrient Medium ◽  
Somatic Embryos ◽  
Single Cells ◽  
Lolium Multiflorum ◽  
Embryogenic Cell Suspension ◽  
Embryogenic Cell ◽  
Meristematic Cells

The embryogenic cell suspension was obtained from immature embryos of <em>Lolium multiflorum</em> through a callus culture. Somatic embryogenesis was induced by addition of 2,4-D, dicamba and picloram in 0,5 mg/l concentrations in MS liquid nutrient medium. It was shown that somatic embryos arised from single cells. In globular embryoids, the meristematic cells are characterized by the presence of phytoferritin inclusions in the leucoplasts.

scholarly journals Cell suspension culture as a means to produce polyphenols from date palm (Phoenix dactylifera L.)

2018 ◽  
Vol 42 (5) ◽  
pp. 464-473 ◽  
Author(s):  
Poornananda Madhava Naik ◽  
Jameel Mohammed Al-Khayri
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Caffeic Acid ◽  
Cell Suspension Culture ◽  
Date Palm ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Phoenix Dactylifera ◽  
Fresh Weight ◽  
Phoenix Dactylifera L

ABSTRACT Date palm accumulates a wide range of secondary metabolites high in nutritional and therapeutic value. In the present study, date palm (Phoenix dactylifera L., cv. Shaishi) shoot-tip-induced callus was used to establish cell suspension cultures in Murashige and Skoog (MS) liquid medium containing 1.5 mg L-1 2-isopentenyladenine (2iP) and 10 mg L-1 naphthaleneacetic acid (NAA). To study the growth kinetics, cultures were maintained for 12 weeks during which weekly measurements were carried out to determine the biomass accumulation based on packed cell volume (%), fresh weight and dry weight (g). In addition, weekly determination of polyphenols (catechin, caffeic acid, kaempferol, and apigenin) was carried out using high performance liquid chromatography (HPLC). The 11-week-old culture was found highest in the production of biomass (62.9 g L-1 fresh weight and 7.6 g L-1 dry weight) and polyphenols (catechin-155.9 µg L-1, caffeic acid-162.7 µg L-1, kaempferol-89.7 µg L-1, and apigenin-242.7 µg L-1) from the cell suspension cultures. This is the first report on the production of polyphenols from the cell suspension culture of date palm. This study facilitates further development of large-scale production of polyphenols and the utilization of bioreactors.

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