scholarly journals Effect of Nimodipine on Premature Luteinizing Hormone Surge in Women Undergoing Intrauterine Insemination

2020 ◽  
Vol 8 (2) ◽  
pp. 165-168
Author(s):  
Zahra Razghandi ◽  
Robabeh Taheripanah ◽  
Zahra Heidar
Keyword(s):  
Placebo Group ◽  
Luteinizing Hormone ◽  
Statistical Difference ◽  
Intrauterine Insemination ◽  
Serum Levels ◽  
Lh Surge ◽  
Infertile Women ◽  
Before And After ◽  
Premature Lh Surge ◽  
Luteinizing Hormone Surge

Objectives: To determine the effect of nimodipine on premature luteinizing hormone (LH) surge in women undergoing intrauterine insemination (IUI). Patients and Methods: Fifty-six infertile women participated in this randomized clinical trial after referring to Mahdiyeh hospital, Tehran, Iran and undergoing IUI treatment in 2017. Participants were randomly divided into nimodipine (n=34) and placebo (n=22) groups. The demographic and clinical profile of women were collected using a predesigned checklist. In the nimodipine group, 30 mg tablets were given to patients three times daily for 2 days. Finally, the serum levels of LH and estradiol were measured before and after the intervention. Results: Based on the results, the LH surge was observed in 8 (34.8%) women in the placebo group (P=0.04) while it was not detected in 29 (78.4%) women in the nimodipine group. There were no statistical differences in the serum levels of estradiol and LH between the 2 groups before the intervention. The serum levels of estradiol in both groups increased after intervention although this increase was not significant. Eventually, no statistical difference was found between the 2 groups in terms of fertility rate. Conclusions: In general, nimodipine can significantly reduce premature LH surge in patients undergoing IUI compared to the placebo group.

scholarly journals Effects of Letrozole in Prevention of Premature Luteinizing Hormone (LH) Surge in Infertile Women with Clomiphene Citrate Resistant Polycystic Ovary Syndrome (PCOS) Undergoing Intrauterine Insemination

2015 ◽  
Vol 4 (3) ◽  
pp. 104-11
Author(s):  
Afsoon Zarei ◽  
Tahere Bahrami Shabahrami ◽  
Nasrin Dadras
Keyword(s):  
Luteinizing Hormone ◽  
Pregnancy Rate ◽  
Polycystic Ovary ◽  
Endometrial Thickness ◽  
Intrauterine Insemination ◽  
Clomiphene Citrate ◽  
Control Group ◽  
Lh Surge ◽  
Significant Difference ◽  
Premature Lh Surge

Background: Polycystic ovarian syndrome (PCOS) is among the important causes of infertility in young women. Premature luteinizing hormone (LH) surge (PLS) is one of its complications. PLS can reduce the quality of oocytes and therefore decrease the success of intrauterine insemination (IUI). Letrozole, a non-steroidal aromatase inhibitor, prevents LH surge. In this study, we aim to evaluate the effects of letrozole on preventing premature LH surge in clomiphene-resistant patients with PCOS undergoing IUI. Materials and Methods: In this randomized clinical trial, 131 patients who were developed with PCOS were selected for IUI cycle, divided into two groups randomly: control group (n=67) and letrozole group (n=64). Incidence of premature LH surge, pregnancy, abortion and ongoing pregnancy rate, endometrial thickness and number of follicles were measured in both groups. Results: No significant difference was seen between mean ages in the two groups; 11.9% of the control group and 21.9% of the letrozole group became pregnant (P =0.005); furthermore, premature LH surge was seen in 4.7% of the letrozole group and 8.9% of the control group (P =0.003). E2 and Endometrial thickness was higher in letrozole group; however, LH was significantly higher in the control group (P =0.026). Conclusion: Administration of letrozole in clomiphene-resistant patients with PCO undergoing IUI cycle can decrease the incidence of PLS. In addition, it can increase pregnancy rate significantly. Therefore, using letrozole is more reasonable in patients who have not responded to clomiphene or are hypersensitive. [GMJ.2015;4(3):104-11]

CHANGES IN SERUM LEVELS OF FOLLICLE STIMULATING HORMONE AND LUTEINIZING HORMONE SHORTLY BEFORE AND AFTER PARTURITION IN RATS

1974 ◽  
Vol 75 (3) ◽  
pp. 491-496 ◽  
Author(s):  
Junichi Mori ◽  
Hiroshi Nagasawa ◽  
Reiko Yanai ◽  
Junji Masaki
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Serum Levels ◽  
Sprague Dawley ◽  
Appreciable Change ◽  
Serum Lh ◽  
Sprague Dawley Rats ◽  
Before And After ◽  
Average Serum ◽  
Stimulating Hormone

ABSTRACT The sequence of changes in the serum levels of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from 2 days before to 24 h after parturition of primiparous Sprague-Dawley rats was investigated by radioimmunoassay. No appreciable change in average serum FSH levels was observed during 2 days before and 1 h after parturition. After this the levels increased gradually to show a peak at 7 h after parturition and then declined gradually until 24 h after parturition. However, the level at 24 h after parturition was still twice as high as that at parturition (0 h). The average serum LH levels which were low between 2 days before and 1 h after parturition, showed a peak at 7 h and decreased toward 13 h after parturition. The same levels as at parturition were maintained between 13 and 24 h after parturition. The time of surge of either FSH or LH was closely related to the time after parturition. There were some differences between FSH and LH in the patterns of sequence of changes in the serum levels near parturition.

EFFECTS OF MATING AND INJECTION OF LUTEINIZING HORMONE RELEASING HORMONE ON SERUM LUTEINIZING HORMONE AND TESTOSTERONE CONCENTRATIONS IN RABBITS

1978 ◽  
Vol 76 (3) ◽  
pp. 417-425 ◽  
Author(s):  
C. A. BLAKE ◽  
PATRICIA K. BLAKE ◽  
NANCY K. THORNEYCROFT ◽  
I. H. THORNEYCROFT
Keyword(s):  
Luteinizing Hormone ◽  
Serum Testosterone ◽  
Serum Levels ◽  
Transient Increase ◽  
Marked Rise ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Serum Lh ◽  
Before And After ◽  
Lh Rh

The effects of coitus and injection of luteinizing hormone releasing hormone (LH-RH) on serum concentrations of LH, testosterone and dihydrotestosterone (17β-hydroxy-5α-androstan-3-one; DHT) were tested in male rabbits. Before experimentation, male and female rabbits were housed in individual cages in the same room. Male rabbits were then bled by cardiac puncture before and after placement with female rabbits or intravenous injection of LH-RH. Serum LH, testosterone and DHT were measured by radioimmunoassay. Sexual excitement (sniffing, chasing and mounting), with or without intromission, caused a marked rise in serum testosterone and DHT concentrations in only some of the bucks. These increases were accompanied or preceded by a small, transient increase in serum LH. In the rest of the bucks, sexual excitement with or without intromission had either no effect on serum levels of all three hormones, or only serum testosterone and DHT decreased during the collection period. Similar responses were measured in bucks which were housed in a room without does for 2–4 weeks before experimentation. Injection of 10, 30 or 100 ng or 50 μg LH-RH caused serum LH, testosterone and DHT to rise in all bucks tested, but the magnitude of the rises in serum testosterone and DHT were not related to the magnitude of the LH rise. In both mated and LH-RH-injected bucks, the rises in serum testosterone and DHT were greatest in animals with low initial testosterone and DHT values. Under the conditions of this study, the data suggest that: (1) serum testosterone and DHT rise in only some male rabbits after sexual excitement (with or without intromission), (2) the rises in serum testosterone and DHT are dependent on a small transient increase in serum LH and (3) sexual excitement is less likely to cause release of LH-RH in bucks with raised serum testosterone and DHT concentrations.

CONTROL OF THE PREOVULATORY SURGE OF LUTEINIZING HORMONE IN THE HEN (GALLUS DOMESTICUS): THE ROLE OF PROGESTERONE AND ANDROGENS

1978 ◽  
Vol 77 (1) ◽  
pp. 57-65 ◽  
Author(s):  
J. B. WILLIAMS ◽  
P. J. SHARP
Keyword(s):  
Luteinizing Hormone ◽  
Dark Period ◽  
Ovarian Follicle ◽  
Gallus Domesticus ◽  
Ovulatory Cycle ◽  
Blood Samples ◽  
Lh Surge ◽  
Before And After ◽  
Small Rise

Peripheral blood samples were taken from laying hens at frequent intervals during various periods of the ovulatory cycle in order to detect small changes in the concentrations of progesterone and androgen which might be important in initiating the preovulatory release of LH. Blood samples were taken from seven hens at 1 h intervals for 3 h when the ovary contained a mature (C1) follicle and on another occasion, when the largest ovarian follicle was immature. The concentrations of progesterone and androgens in the plasma were 30% higher when there was a mature C1 follicle present in the ovary than when there was not, but this increase was significant (P < 0·05) only for progesterone. The concentrations of progesterone and androgens were also measured in blood samples taken at 30 min intervals during the 3 h before and after the initiation of the first preovulatory LH surge of a sequence. The hens were kept on a lighting schedule of 14 h light/day and the first LH surge of a sequence was initiated at the beginning of the dark period. Just after the onset of darkness there was a small increase in the concentration of LH in the plasma and a subsequent, larger preovulatory release of LH. The first increase in the level of LH was associated with a small rise in the concentrations of androgens and progesterone in the plasma while the preovulatory release of LH was accompanied by a much larger increase in the secretion of these steroids. It is proposed that the increase in the level of LH in the plasma at the onset of darkness stimulates the maturing ovarian follicles to secrete progesterone and androgens and that the quantities of these steroids secreted (particularly of progesterone) depends on the maturity of the largest ovarian follicle. If the largest ovarian follicle is mature, then the increase in the level of LH in the plasma associated with the onset of darkness stimulates the secretion of a quantity of progesterone sufficient to cause the preovulatory surge of LH. A diurnal increase in the concentration of LH in the plasma could, therefore, be responsible for timing the preovulatory surges of LH so that they are only initiated at night.

Determination of optimal time for mating by artificial insemination with chilled semen using luteinizing hormone surge as an indicator in beagles

1999 ◽  
Vol 35 (4) ◽  
pp. 348-352 ◽  
Author(s):  
T Nishiyama ◽  
T Kinugasa ◽  
T Kimura ◽  
G Watanabe ◽  
K Taya ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Artificial Insemination ◽  
Sperm Count ◽  
Egg Yolk ◽  
Optimal Time ◽  
Lh Surge ◽  
Serum Lh ◽  
The Mean ◽  
Luteinizing Hormone Surge

Artificial insemination (AI) was conducted using the second fraction of semen, which was collected from 15 male dogs, diluted to a total sperm count of 100x10(6) for each insemination with egg-yolk Tris (eyT) citrate acid buffer and incubated at 4 degrees C for 48 hours. Luteinizing hormone (LH) surge was detected to determine the optimal time for mating using canine LH assay kits. Artificial insemination using 100x10(6) sperm was performed on the fourth and sixth days or the fifth and seventh days after the LH surge. The conception rates were 33% (4/12) and 89% (8/9), respectively; the whelping rates also showed similar results. Serum LH and follicle stimulating hormone (FSH) concentrations were measured in nine dogs, and the mean LH concentration (+/- standard deviation) at LH surge was 15.77+/-7.66 ng/ml. The time of the LH surge detected by the canine LH assay kit was very similar to that measured by radioimmunoassay (RIA).

PREOVULATORY LEVELS OF LUTEINIZING HORMONE, THE CRITICAL PERIOD AND OVULATION IN RATS

1973 ◽  
Vol 57 (2) ◽  
pp. 235-245 ◽  
Author(s):  
FENELLA GREIG ◽  
JUDITH WEISZ
Keyword(s):  
Luteinizing Hormone ◽  
Plasma Levels ◽  
Critical Period ◽  
Lh Surge ◽  
Cardiac Puncture ◽  
Before And After ◽  
Small Rise ◽  
Per Se ◽  
Serial Samples

SUMMARY Plasma levels of luteinizing hormone (LH) were measured by radioimmunoassay in serial samples obtained from unanaesthetized, unrestrained rats by means of intracardiac cannulae (i) in relation to the critical period of neural facilitation (CP) during the afternoon of pro-oestrus and (ii) before and after an injection of sodium pentobarbitone given at specific times in relation to the critical period. Samples were also obtained by cardiac puncture before and after hypophysectomy carried out at specific times in relation to the CP. The effect of pentobarbitone or hypophysectomy on ovulation was also determined in the same animals. The CP as defined by pentobarbitone injection and hypophysectomy lasted from 15.00 to 16.00 h in the majority of rats. During this period the LH levels rose gradually above the basal value, and rose sharply thereafter to reach peak values at 17.00–18.00 h. Pentobarbitone given before the CP delayed the surge by 24 h, while its administration at the end of the CP caused an abrupt fall in plasma LH, indicating a reliance on the neurogenic stimulus both to initiate and to maintain the LH surge. LH levels reached immediately after pentobarbitone administration or hypophysectomy were correlated with the subsequent pattern of ovulation. Only a small increase in LH, amounting to about 14% of the peak values attained during the afternoon of prooestrus, was required for ovulation per se. This amount is similar to the small rise in circulating LH observed during the CP. The bulk of LH secreted during the 'pre-ovulatory surge' does not appear to be required for ovulation.

EFFECT OF PENTOBARBITAL ANAESTHESIA ON LUTEINIZING HORMONE LEVELS IN INTACT AND OVARIECTOMIZED EWES

1975 ◽  
Vol 80 (1) ◽  
pp. 32-41 ◽  
Author(s):  
Allen R. Ellicott ◽  
Henry J. Benoit ◽  
Rudi Borth ◽  
Ronald C. Strickler ◽  
C. Allan Woolever
Keyword(s):  
Luteinizing Hormone ◽  
Control Period ◽  
Serum Levels ◽  
Regulatory Mechanisms ◽  
Time Pattern ◽  
Lh Surge ◽  
Deep Anaesthesia ◽  
Lh Secretion ◽  
Light Sleep ◽  
Pentobarbital Anaesthesia

ABSTRACT In six intact ewes, deep anaesthesia lasting 1—2 h was induced with pentobarbital given intravenously at the onset of oestrus. In a second group of six ovariectomized ewes, light pentobarbital anaesthesia was induced following a control period of 4 h and then maintained for 2, 3, or 4 h. No drug was given to control animals (seven in the first group, three in the second). Serum levels of luteinizing hormone (LH) were determined by radioimmunoassay hourly in the first group, and at intervals of 5 or 10 min in the second. In the oestrous ewes, pentobarbital did not prevent or modify the expected LH surge. In the ovariectomized ewes, the drug, while not affecting the pulsatile release pattern of LH, reduced average LH levels to about half their control values (P < 0.001) when given in a dosage maintaining 3 or 4 h of light sleep; the 2-h dosage had no such effect. These differential effects of pentobarbital indicate that LH secretion in ewes is regulated by more than one mechanism. In particular, our findings are consistent with a hypothesis that there are, in ovariectomized ewes, independent central regulatory mechanisms for the amount of LH released, and for the time pattern of its release.

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