Identification of unknowns by melting point and thin-layer chromatography in combination

1990 ◽  
Vol 67 (11) ◽  
pp. 972 ◽  
Author(s):  
Samuel G. Levine
Keyword(s):  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

scholarly journals SINTESIS CALKON ((E)-1,3-DI(NAPHTHALEN-2-YL)PROP-2-EN-1-ONE) DAN AKTIVITASNYA SEBAGAI ANTIOKSIDAN

2012 ◽  
Vol 3 (1) ◽  
pp. 7-14
Author(s):  
Rahmiwati Hilma ◽  
Jasril ◽  
Hilwan Yuda Teruna
Keyword(s):  
Free Radical ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Room Temperature ◽  
Intermediate Compound ◽  
Alkaline Condition ◽  
Point Measurement ◽  
Anti Inflammatory ◽  
Layer Chromatography

Study on chalcone calkon (E)-1,3-di(naphthalen-2-yl)prop-2-en-1-one synthesis have been carried out with stirrer methode. These compounds can be used as intermediate compound to synthesize others compounds which was reported having antimicrobial, anti-inflammatory, anti-depressant, anti-tumour. The of chalcones synthesis vatives were reported in acid and alkali condition. In this study, chalcone and its derivates were synthesized by using stirrer method in alkaline condition in room temperature. the compounds subjected to somes analyses including melting point measurement, thin layer chromatography and HPLC. Scavenging free radical by using DPPH methode showed Scavenging free radical with LC50 >80 μg/ml min potent activity while the ascorbat acid LC50 89,79 μg/ml.

Adding Gas Chromatography–Mass Spectrometry Data to a Melting Point and Thin-Layer Chromatography Laboratory

2012 ◽  
Vol 90 (1) ◽  
pp. 140-141 ◽  
Author(s):  
Adam M. Azman ◽  
Julie A. Barrett ◽  
Megan Darragh ◽  
John J. Esteb ◽  
LuAnne M. McNulty ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mass Spectrometry Data ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Layer Chromatography

A PRACTICAL METHOD FOR ESTIMATION OF URINARY PREGNANEDIOL AND ALLOPREGNANEDIOL USING THIN LAYER CHROMATOGRAPHY

1965 ◽  
Vol 49 (1) ◽  
pp. 97-106 ◽  
Author(s):  
S. Sulimovici ◽  
B. Lunenfeld ◽  
M. C. Shelesnyak
Keyword(s):  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Practical Method ◽  
Melting Point Determination ◽  
Uv Absorption Spectra ◽  
Infra Red ◽  
Point Determination ◽  
Layer Chromatography ◽  
Hydrolysis Of

ABSTRACT A method is described for the assay of urinary pregnanediol and allopregnanediol which employs acid hydrolysis of pregnanediol glucuronide, ascending thin-layer chromatography and spectrophotometric quantitation of the eluted pregnanediol and allopregnanediol. The reliability of the method was established by analysis of infra-red and UV absorption spectra, melting point determination, recovery experiments, duplicate assays and comparison with the Klopper method. The method has advantages in that it is rapid and requires small urine samples.

Sterols produced by Cryptotnonas ovata var. palustris

1968 ◽  
Vol 14 (8) ◽  
pp. 837-839 ◽  
Author(s):  
R. P. Collins ◽  
K. Kalnins
Keyword(s):  
Infrared Spectroscopy ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mass Spectroscopy ◽  
Layer Chromatography

Cholesterol and stigmasterol were isolated from C. ovata var. palustris. The compounds were identified by thin-layer chromatography, mass spectroscopy, infrared spectroscopy, derivative formation, and melting point determinations.

Carbonyl Compounds in Salted Cod.: II. Separation and Identification of Volatile Monocarbonyl Compounds from Heavily Salted Cod

1965 ◽  
Vol 22 (1) ◽  
pp. 27-32 ◽  
Author(s):  
M. Yurkowski ◽  
M.A. Bordeleau
Keyword(s):  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chain Length ◽  
Carbonyl Compounds ◽  
Gadus Morhua ◽  
Unknown Compound ◽  
Layer Chromatography

Volatile monocarbonyl compounds were isolated from heavily salted cod (Gadus morhua) as the 2,4-dinitrophenylhydrazones. These compounds were separated by thin-layer chromatography first into classes and then according to chain length. n-Alkanals C1–5 and n-alk-2-enals C5–6 were conclusively identified. n-Alkanals C8,10 and n-alk-2-enals C4,7,8 were tentatively identified. An unknown compound corresponding to n-hexanal, except in the melting point, was isolated. Formaldehyde, and to a lesser extent, acetaldehyde and propanal, were the major monocarbonyls present in salted cod.

scholarly journals Synthesis of quaternary ammonium salts based on quinuclidin-3-ol and pyridine-4-aldoxime with alkyl chains

St open ◽  
2020 ◽  
Vol 1 ◽  
pp. 1-8
Author(s):  
Doris Crnčević ◽  
Renata Odžak
Keyword(s):  
Melting Point ◽  
Nitrogen Atom ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Antimicrobial Agents ◽  
Ftir Spectra ◽  
Ammonium Salts ◽  
Product Yields ◽  
Alkyl Chains ◽  
Layer Chromatography

Aim: In search of a new class of potential antimicrobial agents, novel ammonium salts based on quinuclidine-3-ol and pyridine-4-aldoxime with different lengths of alkyl chains were synthesized and analyzed. In addition to their potential biological application, newly synthesized salts containing terminal bromine could possibly be used for the synthesis of more potent bisquaternary salts. Methods: The commercially available quinuclidine-3-ol and pyridine-4-aldoxime were used for the synthesis of new derivatives with the appropriate alkyl chains. The purity of the synthesized salts was tested by thin-layer chromatography on an aluminum plate where aluminum oxide was used as a stationary phase. Melting points were determined in open capillaries using an instrument for melting point determination and the obtained values were left uncorrected. The FTIR spectra were recorded with a spectrometer and the data were analyzed in cm-1. Results: All the synthesized compounds, which contained heterocyclic moiety and alkyl chains (with or without a terminal bromine atom), were obtained in very good yields under the simple quaternization conditions. The better product yields were observed in reactions with quinuclidine3-ol (46-95%) compared to those with pyridine-4-aldoxime (49-59%). The obtained products were analyzed and confirmed by the thin-layer chromatography, the melting point measurement and the FTIR spectra. Conclusion: The observed difference in product yields could be explained by the different basicity of the nitrogen atom of quinuclidin-3-ol than that of pyridine-4-aldoxime. All prepared salts have a positively charged nitrogen atom as a part of their polar “head” and a long hydrocarbon chain as the non-polar “tail”. Such a structure allows electrostatic interaction with the negatively charged bacterial membrane and causes it to be disrupted. The structure of the prepared salts containing long alkyl chains could be crucial for their antimicrobial activity.

scholarly journals Gartanin Compounds from Extract Ethanol Pericarp Mangosteen (Garcinia mangostana Linn.)

2019 ◽  
Vol 7 (22) ◽  
pp. 3891-3895
Author(s):  
R.A. Oetari ◽  
Hasriyani Hasriyani ◽  
Adi Prayitno ◽  
Sahidin Sahidin
Keyword(s):  
Liquid Chromatography ◽  
Melting Point ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Secondary Metabolite ◽  
13C Nmr ◽  
Separation And Purification ◽  
Garcinia Mangostana ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography

AIM: The study aimed to isolate and identification secondary metabolite from pericarp Garcinia mangostana Linn. METHODS: The first step of this research was maceration of sample using alcohol 70% solvent. The separation and purification of compounds using Vacuum Liquid Chromatography (VLC), Radial Chromatography (RC). The purity of isolate was analyzed by thin layer chromatography (TLC) and melting point. Compounds identified using spectroscopi IR, NMR-1D (1H, 13C-NMR and DEPT) and 2-D NMR (HMQC and HMBC). RESULTS: The compound has melting point at 165-167°C. The result showed isolate was gartanin. CONCLUSION: The secondary metabolite found in pericarp Garcinia mangostana Linn. is gartanin.

Identification of chemotypesin Holy Basil (Ocimum sanctum) by High Performance Thin Layer Chromatography (HPTLC)

Planta Medica ◽  
2014 ◽  
Vol 80 (16) ◽  
Author(s):  
T Schinle ◽  
I Merfort ◽  
D Frommenwiler ◽  
E Reich
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Ocimum Sanctum ◽  
Layer Chromatography ◽  
Holy Basil
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