scholarly journals Low-protein diet in puberty impairs testosterone output and energy metabolism in male rats

2018 ◽  
Vol 237 (3) ◽  
pp. 243-254 ◽  
Author(s):  
Júlio Cezar de Oliveira ◽  
Egberto Gaspar de Moura ◽  
Rosiane Aparecida Miranda ◽  
Ana Maria Praxedes de Moraes ◽  
Luiz Felipe Barella ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Hpa Axis ◽  
Visceral Fat ◽  
Protein Diet ◽  
Male Rats ◽  
Low Protein Diet ◽  
Low Protein ◽  
Insulin Homeostasis ◽  
Normal Protein Diet

We examined the long-term effects of protein restriction during puberty on the function of hypothalamic–pituitary–adrenal (HPA) and hypothalamic–pituitary–gonadal (HPG) axes in male rats. Male Wistar rats from the age of 30 to 60 days were fed a low-protein diet (4%, LP). A normal-protein diet (20.5%) was reintroduced to rats from the age of 60 to 120 days. Control rats were fed a normal-protein diet throughout life (NP). Rats of 60 or 120 days old were killed. Food consumption, body weight, visceral fat deposits, lipid profile, glycemia, insulinemia, corticosteronemia, adrenocorticotropic hormone (ACTH), testosteronemia and leptinemia were evaluated. Glucose-insulin homeostasis, pancreatic-islet insulinotropic response, testosterone production and hypothalamic protein expression of the androgen receptor (AR), glucocorticoid receptor (GR) and leptin signaling pathway were also determined. LP rats were hypophagic, leaner, hypoglycemic, hypoinsulinemic and hypoleptinemic at the age of 60 days (P < 0.05). These rats exhibited hyperactivity of the HPA axis, hypoactivity of the HPG axis and a weak insulinotropic response (P < 0.01). LP rats at the age of 120 days were hyperphagic and exhibited higher visceral fat accumulation, hyperleptinemia and dyslipidemia; lower blood ACTH, testosterone and testosterone release; and reduced hypothalamic expression of AR, GR and SOCS3, with a higher pSTAT3/STAT3 ratio (P < 0.05). Glucose-insulin homeostasis was disrupted and associated with hyperglycemia, hyperinsulinemia and increased insulinotropic response of the pancreatic islets. The cholinergic and glucose pancreatic-islet responses were small in 60-day-old LP rats but increased in 120-day-old LP rats. The hyperactivity of the HPA axis and the suppression of the HPG axis caused by protein restriction at puberty contributed to energy and metabolic disorders as long-term consequences.

Cholinergic-pathway-weakness-associated pancreatic islet dysfunction: a low-protein-diet imprint effect on weaned rat offspring

2020 ◽  
Vol 11 (5) ◽  
pp. 484-491 ◽  
Author(s):  
Paulo Cezar de Freitas Mathias ◽  
Ginislene Dias Souza Miranda ◽  
Luiz Felipe Barella ◽  
Rosiane Aparecida Miranda ◽  
Audrei Pavanello ◽  
...  
Keyword(s):  
Vagus Nerve ◽  
Pancreatic Islet ◽  
Muscarinic Acetylcholine Receptor ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Suckling Period ◽  
Rat Offspring ◽  
Low Protein ◽  
Muscarinic Response ◽  
Insulin Homeostasis

AbstractCurrently, metabolic disorders are one of the major health problems worldwide, which have been shown to be related to perinatal nutritional insults, and the autonomic nervous system and endocrine pancreas are pivotal targets of the malprogramming of metabolic function. We aimed to assess glucose–insulin homeostasis and the involvement of cholinergic responsiveness (vagus nerve activity and insulinotropic muscarinic response) in pancreatic islet capacity to secrete insulin in weaned rat offspring whose mothers were undernourished in the first 2 weeks of the suckling phase. At delivery, dams were fed a low-protein (4% protein, LP group) or a normal-protein diet (20.5% protein, NP group) during the first 2 weeks of the suckling period. Litter size was adjusted to six pups per mother, and rats were weaned at 21 days old. Weaned LP rats presented a lean phenotype (P < 0.01); hypoglycaemia, hypoinsulinaemia and hypoleptinaemia (P < 0.05); and normal corticosteronaemia (P > 0.05). In addition, milk insulin levels in mothers of the LP rats were twofold higher than those of mothers of the NP rats (P < 0.001). Regarding glucose–insulin homeostasis, weaned LP rats were glucose-intolerant (P < 0.01) and displayed impaired pancreatic islet insulinotropic function (P < 0.05). The M3 subtype of the muscarinic acetylcholine receptor (M3mAChR) from weaned LP rats was less responsive, and the superior vagus nerve electrical activity was reduced by 30% (P < 0.01). A low-protein diet in the suckling period malprogrammes the vagus nerve to low tonus and impairs muscarinic response in the pancreatic β-cells of weaned rats, which are imprinted to secrete inadequate insulin amounts from an early age.

scholarly journals Does a Supplemental Low-Protein Diet Decrease Mortality and Adverse Events After Commencing Dialysis? A Nationwide Cohort Study

Nutrients ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 1035 ◽  
Author(s):  
Chieh-Li Yen ◽  
Kun-Hua Tu ◽  
Ming-Shyan Lin ◽  
Su-Wei Chang ◽  
Pei-Chun Fan ◽  
...  
Keyword(s):  
Protein Diet ◽  
Low Protein Diet ◽  
Research Database ◽  
Negative Consequences ◽  
Term Survival ◽  
Low Protein ◽  
All Cause Mortality ◽  
Stage Renal Disease ◽  
End Stage

Background: A beneficial effect of a ketoanalogue-supplemented low-protein diet (sLPD) in postponing dialysis has been demonstrated in numerous previous studies. However, evidence regarding its effect on long-term survival is limited. Our study assessed the long-term outcomes of patients on an sLPD after commencing dialysis. Methods: This retrospective study examined patients with new-onset end-stage renal disease with permanent dialysis between 2001 and 2013, extracted from Taiwan’s National Health Insurance Research Database. Patients who received more than 3 months of sLPD treatment in the year preceding the start of dialysis were extracted. The outcomes studied were all-cause mortality, infection rate, and major cardiac and cerebrovascular events (MACCEs). Results: After propensity score matching, the sLPD group (n = 2607) showed a lower risk of all-cause mortality (23.1% vs. 27.6%, hazard ratio (HR) 0.77, 95% confidence interval (CI) 0.70–0.84), MACCEs (19.2% vs. 21.5%, HR 0.86, 95% CI 0.78–0.94), and infection-related death (9.9% vs. 12.5%, HR 0.76, 95% CI 0.67–0.87) than the non-sLPD group did. Conclusion: We found that sLPD treatment might be safe without long-term negative consequences after dialysis treatment.

Effects of Long-Term Low Protein Diet on Albumin Metabolism in Chronic Uremia

Nephron ◽  
1975 ◽  
Vol 15 (6) ◽  
pp. 409-423 ◽  
Author(s):  
R. Bianchi ◽  
G. Mariani ◽  
A. Pilo ◽  
M.G. Toni
Keyword(s):  
Protein Diet ◽  
Low Protein Diet ◽  
Low Protein ◽  
Chronic Uremia

scholarly journals Long-term outcome on renal replacement therapy in patients who previously received a keto acid–supplemented very-low-protein diet

2009 ◽  
Vol 90 (4) ◽  
pp. 969-974 ◽  
Author(s):  
Philippe Chauveau ◽  
Lionel Couzi ◽  
Benoit Vendrely ◽  
Valérie de Précigout ◽  
Christian Combe ◽  
...  
Keyword(s):  
Renal Replacement Therapy ◽  
Replacement Therapy ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Keto Acid ◽  
Term Outcome ◽  
Long Term Outcome ◽  
Low Protein ◽  
Very Low Protein Diet

scholarly journals Post-ruminal effects of rumen-protected methionine supplementation with low protein diet using long-term simulation and in vitro digestibility technique

AMB Express ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Imtiaz Hussain Raja Abbasi ◽  
Farzana Abbasi ◽  
Mohamed E. Abd El-Hack ◽  
Ayman A. Swelum ◽  
Junhu Yao ◽  
...  
Keyword(s):  
Protein Diet ◽  
Low Protein Diet ◽  
In Vitro Digestibility ◽  
Low Protein ◽  
Methionine Supplementation

Maternal low-protein diet during lactation combined with early overfeeding impair male offspring’s long-term glucose homeostasis

Endocrine ◽  
2018 ◽  
Vol 63 (1) ◽  
pp. 62-69 ◽  
Author(s):  
Douglas Lopes Almeida ◽  
Fernando Salgueiro Simões ◽  
Lucas Paulo Jacinto Saavedra ◽  
Ana Maria Praxedes Moraes ◽  
Camila Cristina Ianoni Matiusso ◽  
...  
Keyword(s):  
Glucose Homeostasis ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Low Protein

Development of β-cell mass in fetuses of rats deprived of protein and/or energy in last trimester of pregnancy

2002 ◽  
Vol 283 (3) ◽  
pp. R623-R630 ◽  
Author(s):  
Eric Bertin ◽  
Marie-Noëlle Gangnerau ◽  
Georges Bellon ◽  
Danièle Bailbé ◽  
Annick Arbelot De Vacqueur ◽  
...  
Keyword(s):  
Control Diet ◽  
Cell Mass ◽  
Energy Restriction ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Restricted Diet ◽  
Β Cell ◽  
Fetal Plasma ◽  
Low Protein

Fetal malnutrition is now proposed as a risk factor of later obesity and type II diabetes. We previously analyzed the long-term impact of reduced protein and/or energy intake strictly limited to the last week of pregnancy in Wistar rats. Three protocols of gestational malnutrition were used: 1) low-protein isocaloric diet (5 instead of 15%) with pair feeding to the mothers receiving the control diet, 2) restricted diet (50% of control diet), and 3) low protein-restricted diet (50% of low-protein diet). Only isolated protein restriction induced a long-term β-cell mass decrease. In the present study, we used the same protocols of food restriction to analyze their short-term impact (on day 21.5 of pregnancy) on β-cell mass development. A 50% β-cell mass decrease was present in the three restricted groups, but low-protein diet, either associated or not to energy restriction, increased fetal β-cell insulin content. Among all the parameters analyzed to further explain our results, we found that the fetal plasma level of taurine was lowered by low-protein diet and was the main predictor of the fetal plasma insulin level ( r = 0.63, P < 0.01). In conclusion, rat fetuses exposed to protein and/or energy restriction during the third part of pregnancy have a similar dramatic decrease in β-cell mass, and their ability to recover β-cell mass development retardation depends on the type of malnutrition used. Moreover, our results support the hypothesis that taurine might play an important role in fetal β-cell mass function.

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