scholarly journals Study using in-vivo binding of 125I-labelled hCG, light and electron microscopy of the repopulation of rat Leydig cells after destruction due to administration of ethylene-1,2-dimethanesulphonate

Reproduction ◽  
1986 ◽  
Vol 76 (1) ◽  
pp. 1-10 ◽  
Author(s):  
N. C. Jackson ◽  
H. Jackson ◽  
J. H. Shanks ◽  
J. S. Dixon ◽  
R. G. Lendon
Keyword(s):  
Electron Microscopy ◽  
Leydig Cells ◽  
Light And Electron Microscopy ◽  
In Vivo Binding

Solving the mechanisms responsible for organelle behavior in vivo by same-cell correlative light and electron microscopy

1992 ◽  
Vol 50 (1) ◽  
pp. 14-15
Author(s):  
Conly L. Rieder
Keyword(s):  
Electron Microscopy ◽  
Quantitative Analysis ◽  
Light Microscopy ◽  
Living Cell ◽  
Light And Electron Microscopy ◽  
Time Behavior ◽  
Dynamic Interactions ◽  
Positive Identification ◽  
Cellular Components

The behavior of many cellular components, and their dynamic interactions, can be characterized in the living cell with considerable spatial and temporal resolution by video-enhanced light microscopy (video-LM). Indeed, under the appropriate conditions video-LM can be used to determine the real-time behavior of organelles ≤ 25-nm in diameter (e.g., individual microtubules—see). However, when pushed to its limit the structures and components observed within the cell by video-LM cannot be resolved nor necessarily even identified, only detected. Positive identification and a quantitative analysis often requires the corresponding electron microcopy (EM).

scholarly journals Dynamics of in vivo ASC speck formation

2017 ◽  
Vol 216 (9) ◽  
pp. 2891-2909 ◽  
Author(s):  
Paola Kuri ◽  
Nicole L. Schieber ◽  
Thomas Thumberger ◽  
Joachim Wittbrodt ◽  
Yannick Schwab ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Death ◽  
Light And Electron Microscopy ◽  
Three Dimensional ◽  
Inflammasome Activation ◽  
Caspase Activation ◽  
Endogenous Gene ◽  
Pyrin Domain ◽  
Effector Caspase

Activated danger or pathogen sensors trigger assembly of the inflammasome adaptor ASC into specks, large signaling platforms considered hallmarks of inflammasome activation. Because a lack of in vivo tools has prevented the study of endogenous ASC dynamics, we generated a live ASC reporter through CRISPR/Cas9 tagging of the endogenous gene in zebrafish. We see strong ASC expression in the skin and other epithelia that act as barriers to insult. A toxic stimulus triggered speck formation and rapid pyroptosis in keratinocytes in vivo. Macrophages engulfed and digested that speck-containing, pyroptotic debris. A three-dimensional, ultrastructural reconstruction, based on correlative light and electron microscopy of the in vivo assembled specks revealed a compact network of highly intercrossed filaments, whereas pyrin domain (PYD) or caspase activation and recruitment domain alone formed filamentous aggregates. The effector caspase is recruited through PYD, whose overexpression induced pyroptosis but only after substantial delay. Therefore, formation of a single, compact speck and rapid cell-death induction in vivo requires a full-length ASC.

Abstract 530: Hemostasis Revisited: a Combined Light and Electron Microscopy Analysis of Hemostatic Plug Formation in vivo

2018 ◽  
Vol 38 (Suppl_1) ◽  
Author(s):  
Maurizio Tomaiuolo ◽  
Chelsea N Matzko ◽  
Izmarie Poventud-Fuentes ◽  
John W Weisel ◽  
Lawrence F Brass ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Plug Formation

Testicular oxytocin: effects of intratesticular oxytocin in the rat

1991 ◽  
Vol 130 (2) ◽  
pp. 231-NP ◽  
Author(s):  
H. D. Nicholson ◽  
S. E. F. Guldenaar ◽  
G. J. Boer ◽  
B. T. Pickering
Keyword(s):  
Leydig Cells ◽  
Light And Electron Microscopy ◽  
Male Rats ◽  
Plasma Testosterone ◽  
Epididymal Sperm ◽  
Long Term Effects ◽  
Sperm Counts ◽  
Term Administration

ABSTRACT The long-term effects of oxytocin administration on the testis were studied using intratesticular implants. Adult male rats had an Accurel device containing 20 μg oxytocin (releasing approximately 200 ng/day) implanted into the parenchyma of each testis; control animals received empty devices. The animals were killed at weekly intervals for 4 weeks. Some animals were perfused and the testes processed for light and electron microscopy. Blood was collected from the remaining animals for the measurement of testosterone, dihydrotestosterone, LH, FSH and oxytocin; epididymal sperm counts were measured and the testes were extracted and radioimmunoassayed for testosterone, dihydrotestosterone and oxytocin. Long-term administration of oxytocin resulted in a significant reduction in testicular and plasma testosterone levels throughout the 4-week period examined and, after 14 days of treatment, lipid droplets were seen in the Leydig cells of treated but not control animals. Concentrations of dihydrotestosterone in the plasma and testes of the oxytocin-treated animals, however, were significantly elevated after 7 and 14 days and at no time fell below control values. Plasma FSH levels were also lower in the oxytocin-treated animals. Intratesticular oxytocin treatment did not affect LH or oxytocin concentrations in the plasma, epididymal sperm counts or the number of Leydig cells in the testis. Empty Accurel devices had no effect on testicular morphology. This study provides the first evidence that oxytocin in vivo can modify steroidogenesis in the testis. Journal of Endocrinology (1991) 130, 231–238

scholarly journals Histopathological and Ultrastructural Changes in the Liver and Gills of the Killifish Aphanius Dispar (Cyprinodontidae) Exposed to Aflatoxin B1

2015 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Horiya H. Al-Azri ◽  
Taher Ba-Omar ◽  
Abdulkadir Elshafie ◽  
Michael J Barry
Keyword(s):  
Electron Microscopy ◽  
Aflatoxin B1 ◽  
Light And Electron Microscopy ◽  
Ultrastructural Changes ◽  
Unusual Behavior ◽  
High Doses

Aflatoxin B1 (AFB1) is a mycotoxin which can cause serious toxicity to animals and humans.  The aim of this study was to investigate the effects of AFB1 in Aphanius dispar fish and measure residues in tissues after in vivo exposure. Aphanius dispar were fed diets containing 50, 100, 150 and 200 µg AFB1/kg for 10, 20 and 30 days. At the end of the experiment, the liver and gills were dissected out and processed for light and electron microscopy. During the experiment, no external changes or unusual behavior were observed in the fish. Histopathological and ultrastructural changes in liver appeared under all four treatments: 50, 100, 150 and 200 µg AFB1/kg. Gill tissues were affected at high doses of 100,150 and 200 µg AFB1/kg. Accumulation of AFB1 residues in liver and gill tissues was found to be related to a dose and duration of exposure.  

Demonstration of pulmonary vascular perfusion by electron and light microscopy

1993 ◽  
Vol 75 (4) ◽  
pp. 1877-1883 ◽  
Author(s):  
M. F. Konig ◽  
J. M. Lucocq ◽  
E. R. Weibel
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Light And Electron Microscopy ◽  
Rabbit Serum ◽  
Capillary Perfusion ◽  
Vascular Perfusion ◽  
Thin Sections ◽  
Gold Particles ◽  
Capillary Recruitment

To estimate the fraction of dense pulmonary capillary network that is perfused under physiological conditions, we developed a new method for the demonstration of in vivo capillary perfusion by light and electron microscopy. Blood plasma was labeled by 8-nm colloidal gold particles coated with rabbit serum albumin. In anesthetized rabbits, 4#x2013;5 ml of this tracer were injected into the right atrium. Two and 15 min later, the circulation was interrupted by a snare around the heart, and the lung was fixed by instillation with glutaraldehyde. Gold particles were found in the plasma space of alveolar capillaries as well as in other organs. A random sample of thin sections studied by electron microscopy revealed that the entire capillary bed of the lung was perfused at least with plasma within 2 min after tracer infusion. Light microscopy of silver-enhanced sections showed areas with different staining intensities but no obviously unperfused capillaries. The concept of capillary recruitment, which would require a significant fraction of capillaries unperfused at rest, may have to be reassessed to consider time factors as well as the two-phase nature of blood; red blood cells and plasma may take different paths.

scholarly journals FORMATION OF BONE TISSUE IN CULTURE FROM ISOLATED BONE CELLS

1974 ◽  
Vol 61 (2) ◽  
pp. 427-439 ◽  
Author(s):  
Itzhak Binderman ◽  
Dan Duksin ◽  
Arieh Harell ◽  
Ephraim Katzir (Katchalski) ◽  
Leo Sachs
Keyword(s):  
Electron Microscopy ◽  
Bone Tissue ◽  
Bone Cells ◽  
Light And Electron Microscopy ◽  
Bone Collagen ◽  
The Matrix ◽  
Three Stages ◽  
And Function

A system is described for the formation of bone tissue in culture from isolated rat bone cells. The isolated bone cells were obtained from embryonic rat calvarium and periosteum or from traumatized, lifted periosteum of young rats. The cells were cultured for a period of up to 8 wk, during which time the morphological, biochemical, and functional properties of the cultures were studied. Formation of bone tissue by these isolated bone cells was shown, in that the cells demonstrated osteoblastic morphology in light and electron microscopy, the collagen formed was similar to bone collagen, there was mineralization specific for bone, and the cells reacted to the hormone calcitonin by increased calcium ion uptake. Calcification of the fine structure of the cells and the matrix is described. Three stages in the calcification process were observed by electron microscopy. It is concluded that these bone cells growing in vitro are able to function in a way similar to such cells in vivo. This tissue culture system starting from isolated bone cells is therefore suitable for studies on the structure and function of bone.

scholarly journals Deficiency of parkin and PINK1 impairs age-dependent mitophagy in Drosophila

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Tom Cornelissen ◽  
Sven Vilain ◽  
Katlijn Vints ◽  
Natalia Gounko ◽  
Patrik Verstreken ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Crucial Role ◽  
Dopaminergic Neurons ◽  
Cultured Cells ◽  
Light And Electron Microscopy ◽  
Muscle Cells ◽  
Age Dependent ◽  
Autophagic Degradation ◽  
Mitochondrial Toxins

Mutations in the genes for PINK1 and parkin cause Parkinson’s disease. PINK1 and parkin cooperate in the selective autophagic degradation of damaged mitochondria (mitophagy) in cultured cells. However, evidence for their role in mitophagy in vivo is still scarce. Here, we generated a Drosophila model expressing the mitophagy probe mt-Keima. Using live mt-Keima imaging and correlative light and electron microscopy (CLEM), we show that mitophagy occurs in muscle cells and dopaminergic neurons in vivo, even in the absence of exogenous mitochondrial toxins. Mitophagy increases with aging, and this age-dependent rise is abrogated by PINK1 or parkin deficiency. Knockdown of the Drosophila homologues of the deubiquitinases USP15 and, to a lesser extent, USP30, rescues mitophagy in the parkin-deficient flies. These data demonstrate a crucial role for parkin and PINK1 in age-dependent mitophagy in Drosophila in vivo.

Correlative light and electron microscopy of primary (9+0) cilia: Response of ciliary varicosities to hypotonic treatment in kidney epithelial cells

1992 ◽  
Vol 50 (1) ◽  
pp. 572-573
Author(s):  
Samuel S. Bowser ◽  
Karen E. Roth ◽  
Conly L. Rieder
Keyword(s):  
Electron Microscopy ◽  
Golgi Apparatus ◽  
Cell Surface ◽  
Primary Cilia ◽  
Apical Cell ◽  
Light And Electron Microscopy ◽  
Kidney Tubule ◽  
Hypotonic Treatment ◽  
Structural Association

Primary cilia are centrosomal appendages which in kidney epithelia extend several micrometers from the apical cell surface into the lumen of the kidney tubule. Although considered by some to be “rudimentary appendages” because they are nonmotile, their intimate structural association with the Golgi apparatus and nucleus suggests to others that they mediate interactions between the cell and its environment. Such a sensory role is not without precedence since various photo- and chemoreceptors are highly modified primary cilia. Despite the near-ubiquitous distribution of primary cilia few studies have addressed their function experimentally, and little detailed information is available regarding their behavior in vivo.

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