scholarly journals Time Course of Morphological Changes in Red Blood Cells and Banked Whole Blood Biochemical Parameters in Different Storage Periods

2013 ◽  
Vol 9 (1) ◽  
pp. 5
Author(s):  
V. V. Moroz ◽  
A. M. Golubev ◽  
E. K. Kozlova ◽  
A. V. Afanasyev ◽  
O. E. Gudkova ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Time Course ◽  
Morphological Changes ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical

scholarly journals Effects of Doxycycline Treatment on Hematological and Blood Biochemical Parameters in Dogs Naturally Infected with Ehrlichia canis

2018 ◽  
Vol 41 (1) ◽  
pp. 99-105 ◽  
Author(s):  
Elena Atanaskova Petrov ◽  
Igor Ulcar ◽  
Irena Celeska ◽  
Ksenija Ilievska ◽  
Pandorce Spasovska Trenkovska ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Ehrlichia Canis ◽  
Severe Thrombocytopenia ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Canine Monocytic Ehrlichiosis ◽  
Significant Difference ◽  
Before And After

Abstract Ehrlichia canis, the etiologic agent of canine monocytic ehrlichiosis (CME), is mainly transmitted by the brown dog tick Rhipicephalus sanguineus. Clinical signs of the disease can be various, depending on the stage of the disease. Typical changes in hematological and blood biochemical parameters are: severe thrombocytopenia, mild to marked non regenerative anaemia and hypoalbuminemia. In order to present the effects of the treatment protocol on several hematological and biochemistry parameters, 34 Ehrlichia canis positive dogs were compared before and after treatment with doxycycline 10mg/kg/day, in duration of four weeks. Besides the clinical sings and laboratory findings, diagnosis was confirmed by antibody tests (Bionote, Korea, AGROLABO S.p.A., Italy). The most common clinical sings were depression, lethargy, pyrexia, vomiting and anorexia. Hematological analyses were performed on the automatic hematology analyzer Exigo EosVet (Sweden), while biochemistry analyses (alanine aminotransferase, aspartate aminotransferase, urea, creatinine, albumin, total protein, globulin and alkaline phosphatase) were performed using the automatic analyzer ChemWell 2910 (Awareness Technology, INC, USA). Statistically significant difference (p<0.05) in hematology changes was present regarding the red blood cells count, platelet count, hematocrit and hemoglobin before and after treatment. Hypoalbuminaemia (Mean 19.21 ±4.96 g/l) was the only serum biochemistry parameter with significant change before and after treatment, as well. Treatment with doxycycline in patients with E.canis resulted in significant increase of hematology parameters (red blood cells, hemoglobin, haematocrit and platelets), as well as albumins in serum.

scholarly journals An Evaluation of Morphological Changes and Deformability of Suspended Red Blood Cells Prepared Using Whole Blood with Different Hemoglobin Levels of Tibetans

2021 ◽  
pp. 1-10
Author(s):  
Rui Zhong ◽  
Dingding Han ◽  
Xiaodong Wu ◽  
Hong Wang ◽  
Wanjing Li ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Whole Blood ◽  
Blood Cells ◽  
Morphological Changes ◽  
Osmotic Fragility ◽  
Spherical Shape ◽  
Hypoxic Environment ◽  
Wide Range ◽  
Group A ◽  
Cell Membrane Protein

Background: The hypoxic environment stimulates the human body to increase the levels of hemoglobin (HGB) and hematocrit and the number of red blood cells. Such enhancements have individual differences, leading to a wide range of HGB in Tibetans’ whole blood (WB). Study Design: WB of male Tibetans was divided into 3 groups according to different HGB (i.e., A: >120 but ≤185 g/L, B: >185 but ≤210 g/L, and C: >210 g/L). Suspended red blood cells (SRBC) processed by collected WB and stored in standard conditions were examined aseptically on days 1, 14, 21, and 35 after storage. The routine biochemical indexes, deformability, cell morphology, and membrane proteins were tested. Results: Mean corpuscular volume, adenosine triphosphate, pH, and deformability were not different in group A vs. those in storage (p > 0.05). The increased rate of irreversible morphology of red blood cells was different among the 3 groups, but there was no difference in the percentage of red blood cells with an irreversible morphology after 35 days of storage. Group C performed better in terms of osmotic fragility and showed a lower rigid index than group A. Furthermore, SDS-PAGE revealed similar cross-linking degrees of cell membrane protein but the band 3 protein of group C seemed to experience weaker clustering than that of group A as detected by Western Blot analysis after 35 days of storage. Conclusions: There was no difference in deformability or morphological changes in the 3 groups over the 35 days of storage. High HGB levels of plateau SRBC did not accelerate the RBC change from a biconcave disc into a spherical shape and it did not cause a reduction in deformability during 35 days of preservation in bank conditions.

scholarly journals Effects of Varying Dietary DL-Methionine Levels on Productive and Reproductive Performance, Egg Quality, and Blood Biochemical Parameters of Quail Breeders

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1839
Author(s):  
Fayiz M. Reda ◽  
Ayman A. Swelum ◽  
Elsayed O.S. Hussein ◽  
Shaaban S. Elnesr ◽  
Ahmad R. Alhimaidi ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Reproductive Performance ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Egg Quality ◽  
Control Group ◽  
Egg Weight ◽  
Blood Biochemical Parameters ◽  
Blood Biochemical ◽  
Kidney Functions

The present study was carried out to study the effects of varying dietary DL-methionine (0, 0.5, 1.5, 2.5, and 3.5 g/kg) levels on the productive and reproductive performance, egg quality and blood biochemical parameters of quail breeders. In total, 150 mature Japanese quails at eight weeks of age were randomly allotted to five groups of 30 for each group. Each group included five replicates, each of six quails (four females and two males). The results showed that egg number, egg weight and egg mass were higher (p < 0.05) with the addition of all DL-methionine levels than that of the control group. Quails from the control group had a lower feed intake (p < 0.001) and a worse feed conversion ratio (FCR) than those from the DL-methionine-treated groups. Supplementation of DL-methionine up to 2.5 g/kg in quail diets increased fertility and hatchability percentages. Birds fed DL-methionine at 1.5 g/kg had the best egg production indices, better FCR and the highest values of fertility and hatchability. Egg weight, yolk %, Haugh unit, egg shape index and unit surface shell weight (USSW) were increased and eggshell % was decreased in quail supplemented with DL-methionine levels compared with the control quail (p < 0.05). Dietary DL-methionine levels did not affect (p > 0.05) the hemoglobin (Hb), red blood cells (RBCs), white blood cells (WBCs) and packed cell volume (PCV) of quails. DL-methionine levels (0.5 and 2.5 g/kg) augmented lymphocytes and basophile (p < 0.05). Low DL-methionine levels (0.5 or 1.5 g/kg) improved liver enzymes and kidney functions. Dietary DL-methionine levels (except 3.5 g/kg) declined serum lactate dehydrogenase (LDH) and decreased lipid profile parameters (except high-density lipoprotein—HDL). Supplementation of DL-methionine at 0.5 and 1.5 g/kg increased immunoglobulin (IgG, IgM and IgA) and superoxide dismutase (SOD), total antioxidant capacity (TAC), catalase (CAT) and reduced glutathione (GSH) (p < 0.001) compared with the control. In conclusion, dietary supplementation of DL-methionine (1.5 g/kg) can enhance the reproductive performance and egg quality of quail breeders. DL-methionine use at levels of 0.5 or 1.5 g/kg improved the liver and kidney functions, lipid profile, immunity and antioxidant parameters of Japanese quail.

Glycophorin A-based exclusion of red blood cells for flow cytometric analysis of platelet glycoprotein expression in citrated whole blood

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Christina Berens ◽  
Johannes Oldenburg ◽  
Bernd Pötzsch ◽  
Jens Müller
Keyword(s):  
Red Blood Cells ◽  
Expression Analysis ◽  
Whole Blood ◽  
Blood Cells ◽  
Control Sample ◽  
Small Sample ◽  
Platelet Glycoprotein ◽  
Glycophorin A ◽  
Giant Platelets ◽  
Platelet Counts

AbstractObjectivesAnalysis of platelet glycoprotein (GP) expression by flow cytometry is applied for diagnostic confirmation of GP-associated thrombocytopathies. While platelet-rich plasma may be used for distinct identification of target events, this strategy is not feasible for small sample volumes or for patients showing low platelet counts and/or giant platelets. However, also the use of whole blood (WB) is hampered by the difficulty to discriminate platelets from red blood cells (RBC) in such patients. To circumvent these limitations, we evaluated the feasibility of a RBC gating-out strategy.MethodsIn addition to platelet GPIb, GPIIa/IIIa, as well as P-selectin (CD62P), citrated whole blood (CWB) samples were stained for RBC-specific glycophorin A (CD235a). CD235a-negative platelet events were further discriminated by forward-/side-scatter characteristics and platelet GP expressions analyzed relative to that of a healthy control sample processed in parallel.ResultsEstablished reference intervals allowed for clear identification of decreased GPIIb/IIIa- or GPIb expression pattern in samples of patients with confirmed Glanzmann thrombasthenia or Bernard–Soulier syndrome, respectively. It could be shown that the analysis of 2,500 platelet events is sufficient for reliable GP expression analysis, rendering the proposed method applicable to samples with low platelet counts.ConclusionsThis study demonstrates the feasibility of CD235a-based exclusion of RBC for platelet GP expression analysis in CWB. In contrast to direct staining of platelet-specific antigens for target identification, this indirect gating out approach is generally applicable independent of any underlying platelet GP expression deficiency.

scholarly journals Hemoglobin Oxidation in Stored Blood Accelerates Hemolysis and Oxidative Injury to Red Blood Cells

2020 ◽  
Vol 12 (04) ◽  
pp. 244-249
Author(s):  
Ibrahim Mustafa ◽  
Tameem Ali Qaid Hadwan
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Changes ◽  
Hemoglobin Concentration ◽  
Red Cell Membrane ◽  
Rbc Membrane ◽  
Hemoglobin Oxidation ◽  
Rbc Indices ◽  
Methemoglobin Formation ◽  
Stored Blood

Abstract Introduction Maintaining blood supply is a challenge in blood banks. Red blood cells (RBCs) stored at 4°C experience issues of biochemical changes due to metabolism of cells, leading to changes collectively referred to as “storage lesions.” Oxidation of the red cell membrane, leading to lysis, contributes to these storage lesions. Methods Blood bags with CPD-SAGM stored at 4°C for 28 days were withdrawn aseptically on days 1, 14, and 28. Hematology analyzer was used to investigate RBC indices. Hemoglobin oxidation was studied through spectrophotometric scan of spectral change. RBC lysis was studied with the help of Drabkin's assay, and morphological changes were observed by light and scan electron microscopy. Results RBCs show progressive changes in morphology echinocytes and spherocytes on day 28. There was 0.85% RBC lysis, an approximately 20% decrease in percentage oxyhemoglobin, and a 14% increase in methemoglobin formation, which shows hemoglobin oxidation on day 28. Conclusions Oxidative damage to RBC, with an increase in storage time was observed in the present study. The observed morphological changes to RBC during the course of increased time shows that there is progressive damage to RBC membrane and a decrease in hemoglobin concentration; percentage RBC lysis is probably due to free hemoglobin and iron.

Sign in / Sign up

Export Citation Format

Share Document