scholarly journals Effectiveness of Endoscopic Application of Type I Human Collagen Solution in Treatment of Inhalation Injury

2018 ◽  
Vol 14 (3) ◽  
pp. 4-14
Author(s):  
I. E. Galankina ◽  
A. V. Makarov ◽  
A. V. Mironov ◽  
S. V. Smirnov ◽  
N. V. Borovkova ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Reference Group ◽  
Repair Process ◽  
Inhalation Injury ◽  
Main Group ◽  
Type I ◽  
Bronchial Mucosa ◽  
Collagen Solution ◽  
Morphological Studies ◽  
Human Collagen

The purpose of the studywas to determine the morphological characteristics of the repair process of the tracheal and bronchial mucosa after sanitation bronchoscopy using type I human  collagen solution  in patients with an inhalation  injury (II).Materials and methods.  An open-label, randomized, prospective study included  59 patients with inhalation injury (2—3 severity  degree according  to the classification  of Yu. V. Sinev and A. Yu. Skripal'.  The main group consisted of 29 patients. They underwent a sanitation bronchoscopy using type I human collagen solution applied onto erosive and ulcerative lesions of the mucous membrane.  The reference group consisted  of 30 patients who underwent sanitation bronchoscopy in accordance with the standards of medical care. In order to assess the morphological  dynamics  of the repair process of the mucous membrane,  a morphological  study  of the bioptate obtained during endoscopic studies was carried out. The solution for the application was prepared by acid extraction of type I human collagen from ligaments and tendons as described  earlier (Russian Federation invention patent No. RU 2591544 C1).Results.Complete  epithelization of the tracheal  and bronchial mucosa occurred  significantly  earlier (4 (3; 7) days) in patients with the 2nd  degree inhalation injury when using type 1 collagen application, than in those without the application (7 (4; 9) days) (n=15 andn=21 respectively;U=49.5;P=0.0004). In the case of the 3rd  degree inhalation injury, coating of ulcers of the tracheobronchial tree mucosa with the collagen solution also significantly reduced the epithelialization period on average to 8 (7; 10) days, while after the standard sanitation bronchoscopy, this period was 17 (12; 22) days (n=14 andn=9  respectively;  U=1;P=0.0001). The morphological  changes in patients of the main group were characterized by the absence of signs of a purulent inflammation, early appearance of macrophages and lymphocytes, and covering of type I collagen by simple cuboidal epithelium, while in the reference group, there was a severe purulent inflammation manifested by polymorphonuclear leukocyte  infiltration.Conclusion.The sanitation bronchoscopy with simultaneous closure of the damaged areas of the mucous membrane with the type I human collagen solution  results  in relief of the inflammatory process which is confirmed by morphological  studies.                                                                                           

scholarly journals THE EFFECT OF EARLY FIBROBRONCHOSCOPIC SANITATION WITH HUMAN COLLAGEN TYPE 1 ON EPITHELIZATION OF DAMAGED TRACHEAL AND BRONCHIAL MUCOSA IN PATIENTS WITH INHALATION INJURY

2018 ◽  
Vol 7 (2) ◽  
pp. 111-116 ◽  
Author(s):  
A. V. Makarov ◽  
A. V. Mironov ◽  
I. Y. Galankina ◽  
S. V. Smirnov ◽  
I. N. Ponomaryov ◽  
...  
Keyword(s):  
Mucous Membrane ◽  
Collagen Type ◽  
Tracheobronchial Tree ◽  
Bronchial Mucosa ◽  
Human Type ◽  
Human Collagen ◽  
Mucosal Lesions ◽  
Collagen Type 1 ◽  
Trachea And Bronchi

Background. Inhalation trauma remains one of the most common and complex condition to be treated. Fibrobronchoscopic sanitation plays a significant role in the complex therapy of patients with inhalation trauma, allowing purulent necrotic discharge, soot and combustion products to be removed from the lumen of the tracheobronchial tree. We proposed a method for local treatment of mucosal lesions in inhalation trauma based on early endoscopic sanitation of the tracheobronchial tree with solutions of antiseptics followed by application of human type 1 collagen solution.Aim of study. To assess the effect of early fibroblochoscopic sanitation with application of human-type collagen 1 on the timing of epithelialization of the damaged tracheal and bronchial mucosa in patients with inhalation trauma.Material and methods. The study included 59 patients with inhalation trauma of 2–3 degree. All patients from the first day after the trauma underwent bronchoscopic sanitation in accordance with the standards of medical care. Immediately after the removal of soot, the solution of human-type collagen 1 was applied in patients of the main group, obtained from ligaments and tendons by the acid extraction method. The dynamics of the reparative process was evaluated on the basis of the endoscopic study and according to a series of morphological studies of the biopsy material obtained in the course of fibrobronchoscopy.Results. Complete epithelization of erosions of the mucous membrane of the trachea and bronchi in patients with inhalation trauma of 2 degree occurred significantly earlier (3 (2; 6) days) than without its application (7 (4; 9) day) (n1 =15; n2 =21; U=49.5; p=0.0004). In patients with inhalation trauma of 3 degree, epithelialization time was reduced from 17 (12; 22) days in the comparison group to 7 (6; 9) days in the main group (n1 =14; n2 =9; U=1; p=0.001). According to the morphological study, a characteristic feature of the fibrobronchoscopic sanitation with the human collagen type 1 was the absence of purulent inflammation of the trachea and bronchial wall.Conclusion. In the study, it was statistically proved that the early fibrobrochoscopic sanitation and application of the human collagen type 1 solution for acute lesions of the mucous membrane of the trachea and bronchi twice accelerates epithelialization of mucosal lesions without the development of purulent inflammation. 

scholarly journals Plant Recombinant Human Collagen Type I Hydrogels for Corneal Regeneration

2021 ◽  
Author(s):  
Michel Haagdorens ◽  
Elle Edin ◽  
Per Fagerholm ◽  
Marc Groleau ◽  
Zvi Shtein ◽  
...  
Keyword(s):  
Collagen Type ◽  
Collagen Type I ◽  
Collagen Fibrils ◽  
Type I ◽  
Safe Alternative ◽  
Human Donor ◽  
Human Collagen ◽  
Donor Corneas

Abstract Purpose To determine feasibility of plant-derived recombinant human collagen type I (RHCI) for use in corneal regenerative implants Methods RHCI was crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to form hydrogels. Application of shear force to liquid crystalline RHCI aligned the collagen fibrils. Both aligned and random hydrogels were evaluated for mechanical and optical properties, as well as in vitro biocompatibility. Further evaluation was performed in vivo by subcutaneous implantation in rats and corneal implantation in Göttingen minipigs. Results Spontaneous crosslinking of randomly aligned RHCI (rRHCI) formed robust, transparent hydrogels that were sufficient for implantation. Aligning the RHCI (aRHCI) resulted in thicker collagen fibrils forming an opaque hydrogel with insufficient transverse mechanical strength for surgical manipulation. rRHCI showed minimal inflammation when implanted subcutaneously in rats. The corneal implants in minipigs showed that rRHCI hydrogels promoted regeneration of corneal epithelium, stroma, and nerves; some myofibroblasts were seen in the regenerated neo-corneas. Conclusion Plant-derived RHCI was used to fabricate a hydrogel that is transparent, mechanically stable, and biocompatible when grafted as corneal implants in minipigs. Plant-derived collagen is determined to be a safe alternative to allografts, animal collagens, or yeast-derived recombinant human collagen for tissue engineering applications. The main advantage is that unlike donor corneas or yeast-produced collagen, the RHCI supply is potentially unlimited due to the high yields of this production method. Lay Summary A severe shortage of human-donor corneas for transplantation has led scientists to develop synthetic alternatives. Here, recombinant human collagen type I made of tobacco plants through genetic engineering was tested for use in making corneal implants. We made strong, transparent hydrogels that were tested by implanting subcutaneously in rats and in the corneas of minipigs. We showed that the plant collagen was biocompatible and was able to stably regenerate the corneas of minipigs comparable to yeast-produced recombinant collagen that we previously tested in clinical trials. The advantage of the plant collagen is that the supply is potentially limitless.

Demonstration by Immunofluorescence That Host Collagen Replaces Dermal Microimplants of Bovine Collagen

1987 ◽  
Vol 4 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Karen E. Burke ◽  
Gail Naughton
Keyword(s):  
Host Response ◽  
Collagen Type ◽  
Type I ◽  
Collagen Type Iii ◽  
Type Iii ◽  
Bovine Collagen ◽  
Collagen Implant ◽  
Human Dermis ◽  
Dermal Collagen ◽  
Human Collagen

It has previously been demonstrated with sequential biopsies analyzed by standard histological stains, immunofluorescence, and electron microscopy that an injectable form of type I bovine dermal collagen (Zyderm® Collagen Implant, or ZCI) in the human dermis stimulates a host response. This host response results in implant degradation and replacement by newly generated host collagen. However, definitive proof that the human collagen is deposited directly on the bovine collagen implants is lacking. This study reports for the first time simultaneous labeling of the ZCI with fluorescein and the host collagen type III with rhodamine. These experiments show that human collagen type III is synthesized directly on the bovine collagen implants.

scholarly journals Hydroxylation of recombinant human collagen type I alpha 1 in transgenic maize co-expressed with a recombinant human prolyl 4-hydroxylase

2011 ◽  
Vol 11 (1) ◽  
pp. 69 ◽  
Author(s):  
Xing Xu ◽  
Qinglei Gan ◽  
Richard C Clough ◽  
Kameshwari M Pappu ◽  
John A Howard ◽  
...  
Keyword(s):  
Collagen Type ◽  
Collagen Type I ◽  
Transgenic Maize ◽  
Type I ◽  
Human Collagen

scholarly journals ASSESSMENT OF BONE METABOLISM IN PATIENTS WITH BISPHOSPHONATE OSTEONECROSIS OF THE JAWS

2017 ◽  
Vol 21 (4) ◽  
pp. 197-199
Author(s):  
Elena Mikhaylovna Spevak ◽  
D. Yu Khristoforando ◽  
A. B Davydov
Keyword(s):  
Bone Metabolism ◽  
Cancer Patients ◽  
Osteonecrosis Of The Jaw ◽  
Main Group ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Type I ◽  
Bone Specific Alkaline Phosphatase ◽  
Osteonecrosis Of The Jaws ◽  
Bisphosphonate Osteonecrosis

The aim of the study was to evaluate bone metabolism in cancer patients with bisphosphonate osteonecrosis of the jaws. The study included 45 people of the main group (patients with cancer with osteonecrosis of the jaw in patients receiving bisphosphonates) and 25 in the control group (cancer patients treated with bisphosphonates, but did not have osteonecrosis of the jaw), which had a stabilization of the underlying disease. Bone metabolism was evaluated by the level of osteocalcin (OC), bone-specific alkaline phosphatase (bALP), aminoterminal of propeptide of procollagen type I (P1NP), tartrate-resistant acid phosphatase (TRAP5b), calcium (Cа), phosphorus (P) in blood serum before treatment and after 6 months. Compared to the average levels of marker patients of the main and control groups using the Mann-Whitney test for p < 0,05. A baseline level of СТХ (0,23±0,02 ng/ml) and OС (11,58±0,54 ng/ml) in the treatment group was significantly (p < 0.05) lower than control group (0,43±0,01 ng/ml and 17,94±0,83 ng/ml), and the level of osteocalcin in the main group (11,58±0,54 ng/ml) was on average below normal 2,59 times. Recorded significantly higher (p < 0,05) levels bALP (133,24±14,03 U/l) and TRAP5b (3,54±0,38 U/l) in patients with osteonecrosis compared with a control group (73,32±3,41 U/l and 3,12±0,12 U/l). Reliably detected differences in the levels of P1NP, Ca and P were not detected (p > 0,05). In the main group after 6 months of treatment was observed a tendency of growth of СТХ, TRAP5b, OK, bALP, P1NP, Ca, but only for the markers of resorption and СТХ, TRAP5b these differences were significant. Indicators of patients in the control group were stable and did not differ in the dynamics. The development of bisphosphonate osteonecrosis of the jaws is directly related to bone metabolism and occurs with predominance of the processes of bone resorption and inhibition of bone formation processes.

scholarly journals Вплив поліморфізму генів PTHR1 та COL1A1 на розвиток захворювань тканин пародонта у людей молодого віку

2017 ◽  
Author(s):  
M. A. Luchynskyi ◽  
Y. V. Boliuk ◽  
V. M. Luchynskyi
Keyword(s):  
Young People ◽  
Periodontal Diseases ◽  
Main Group ◽  
Control Group ◽  
Type I ◽  
Periodontal Tissue ◽  
Endogenous Factors ◽  
Stage Of Development ◽  
The Difference ◽  
Collagen Chain

At the present stage of development of dentistry, the leading Ukrainian and foreign scientists devote a considerable part of the research to a deeper study of the etiology and pathogenetic mechanisms of periodontal tissue diseases and the influence of various exogenous and endogenous factors on their course.The aim of the study – to learn the ability and methods of forecasting and early diagnosis of the periodontal tissue lesions in young people. Materials and Methods. During our research we examined 24 young people with periodontal tissue diseases, who were included to the main group, and 15 healthy people, who formed the control group. The complex clinical examination was performed in each research group. It was studied the distribution of polymorphous variants of the type I parathormone receptor and the α1-chain of collagen gene with a help of polymerase chain reaction by restrictase cleavage of DNA fragments and electrophoresis in polyacrylamide gel (AA/BA 29:1). Results and Discussion. The distribution of genotypes by PTHR1 gene in control group was similar to those in main group (p>0.05). Also we didn’t find the difference between frequencies of the separate alleles in people with periodontal tissue pathology and without it (p>0.05). Yes, the repetitions of the allele 5 encoding normal type I parathormone receptor were found more often, comparing with the allele 6 that is responsible for the formation of unfunctional PTHR1 (р<0.001) in both main and control groups. The dominance of the genotype TT, which corresponds to the pathology, was found in young people with the periodontal tissue lesions – (38.46 ± 4.79) %, while among the control group the genotype of norm GG was met the most often – (68.24±5.08) %. Also, the frequency of repetitions of the allele T encoding the imperfect collagen chain was (57.60±3.79) % in young people with periodontal diseases, and in the control group this figure was (13.27±2.81) %, p<0.001.  Conclusions. According to our results the presence of allele T and genotype TT that correspond the imperfect collagen chain may be one of the causes of periodontal tissue pathology.

scholarly journals Effects of human collagen α-1 type I-derived proteins on collagen synthesis and elastin production in human dermal fibroblasts

BMB Reports ◽  
2021 ◽  
Vol 54 (6) ◽  
pp. 329-334
Author(s):  
Su Jin Hwang ◽  
Su Hwan Kim ◽  
Woo-Young Seo ◽  
Yelin Jeong ◽  
Min Cheol Shin ◽  
...  
Keyword(s):  
Collagen Synthesis ◽  
Dermal Fibroblasts ◽  
Type I ◽  
Human Dermal Fibroblasts ◽  
Human Collagen

scholarly journals Immunohistochemical features of the fallopian tubes’ structure in case of fetuses with a gestational term of 21-28 weeks from mothers with a chronic infection of the lower genital tracts

2021 ◽  
Vol 11 (8) ◽  
pp. 445-456
Author(s):  
V. Poliasnyi ◽  
L. Kupriianova
Keyword(s):  
Mucous Membrane ◽  
Chronic Infection ◽  
Main Group ◽  
Immunohistochemical Method ◽  
Fallopian Tubes ◽  
Tubal Infertility ◽  
Inflammatory Infiltration ◽  
Endocrine Activity ◽  
Endocrine Insufficiency ◽  
Complicated Pregnancy

In the following article we are disclosing immunohistochemical features of the fallopian tubes’ structure in case of fetuses with a gestational term of 21-28 weeks. We have studied the structure of organs of 15 fetuses from mothers with a chronic infection of the lower genital tracts (HILGT) (the group of comparison) relatively to one in case of 15 fetuses from mothers with a physiological pregnancy (the main group). All fetuses had a gestational term of 21-28 weeks and had died intranatally as a result of an acute disorder of uterine-placental circulation. Methods of reearch: organometric, histological, immunohistochemical and statistical. The complex research allowed to reveal a probable decrease of the organometric data as well as indicators of thickness of the main structural components of the organs’ wall in case of fetuses from mothers of the group of comparison relatively to ones in case of fetuses from the main group. By applying histological method we had reveled a disorder in the strucuture of mucous and muscular membranes in the structure of organs of fetuses from mothers with a pathology of pregnancy, which is manifested by decreasing of number of folds, uneven thickness of the mucous membrane as well as disorder in formation of the muscular membrane’ layers. Immunohistochemical method has revealed an increase of apoptotically changed mucosal cells in the fallopian tubes of fetuses from mothers with HILGT. By applying MCAT to endotheline-1 we have revealed an increased glow of endithelial cells in vessels both of arterial and venous types in the strucuture of the organs’ wall in case of fetuses from the group of comparison. During the study on the endocrine activity of organs of fetuses from mothers with complicated pregnancy we have postulated a probable decrease of the cells’ glow’ intensity towards MCAT of progesterone. At the same time, there is also an inflammatory infiltration in the mucous membrane of the fallopian tubes’ wall. The aforementioned changes in the strucuture as well as in the functional activity of fetuses’ organs from mothers’ with HILGT are formed under the influence of chronic hypoxia and endocrine insufficiency of the feto-placental complex, which is taking place in case of this pathology. Histological and immunohistochemical features of the fallopian tubes’ structure in case of fetuses from mothers with complicated pregnancy are indicating a disorder in formation and maturing of the organ as well as they could lead to development of ectopic pregnancy and tubal infertility in the subsequent ontogenesis.

A Simple In Vitro Model of Mechanical Injury of Confluent Cultured Endothelial Cells to Study Quantitatively the Repair Process

1986 ◽  
Vol 56 (02) ◽  
pp. 232-235 ◽  
Author(s):  
Claude Klein-Soyer ◽  
Alain Beretz ◽  
Régine Millon-Collard ◽  
Joseph Abecassis ◽  
Jean-Pierre Cazenave
Keyword(s):  
Endothelial Cells ◽  
In Vitro Model ◽  
Repair Process ◽  
Mechanical Injury ◽  
Brain Extract ◽  
Type I ◽  
Culture Dish ◽  
Vitro Model ◽  
Adhesive Proteins

SummaryA model of in vitro mechanical injury of confluent human endothelial cells (EC) in culture was developed. Human EC were obtained from umbilical veins and grown to confluence. Application on the EC monolayer of a calibrated disk of cellulose poly acetate paper resulted in removal of the EC, leaving a continuous subendothelial extracellular matrix (ECM) on the culture dish. The regeneration time depended on the original size of the lesion. Regeneration was similar with EC grown on different substrates such as human fibronectin, human subendothelial ECM, bovine collagen type I or surfaces coated with Transglutine®, a surgical glue containing adhesive proteins. A human brain extract containing growth factor activity accelerated significantly the repair of the lesion, especially at low serum concentration. This simple in vitro model of mechanical injury allows the quantitative study of the effects of matrices, growth factors and pharmacological agents on the repair process.

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