Potential of Fungicides on Spore Germination And Mycelial Growth of Fruit Rot/Anthracnose Disease of Banana Caused by Colletotrichum Gloeosporioides

2012 ◽  
Vol 3 (6) ◽  
pp. 6-8
Author(s):  
C. S. Aza C. S. Aza ◽  
◽  
J. N. Srivastava J. N. Srivastava ◽  
Gireesh Chand
Keyword(s):  
Spore Germination ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Fruit Rot ◽  
Anthracnose Disease

scholarly journals The effect of fungicides on spore germination, mycelial growth and lesion development of Phlyctema vagabunda (syn: Neofabraea alba) (bull’s eye rot of apples)

2017 ◽  
Vol 70 ◽  
pp. 112-119 ◽  
Author(s):  
P.N. Wood ◽  
B.M. Fisher
Keyword(s):  
Spore Germination ◽  
Mycelial Growth ◽  
Pathogenic Fungus ◽  
Apple Fruit ◽  
Fruit Rot ◽  
Copper Hydroxide ◽  
Lesion Development ◽  
Lime Sulphur ◽  
Bull's Eye

Phlyctema vagabunda (syn: Neofabraea alba) is a plant pathogenic fungus that causes bull’s eye rot on apples and pears. Phlyctema vagabunda fruit infections occur in orchards predominantly pre-harvest, and eventually express as a fruit rot after 4—5 months of cool storage. Twelve fungicides (captan, carbendazim, copper hydroxide, cyprodinil, difenoconazole, dithianon, dodine, isopyrazam, metiram, lime sulphur, sulphur and trifloxystrobin) were tested in vitro for their effects on spore germination and mycelial growth of P. vagabunda. Spore germination was inhibited by metiram, captan, dodine, dithianon, lime sulphur, carbendazim and isopyrazam, in order of effectiveness. Carbendazim, isopyrazam, difenoconazole and cyprodinil, in order of effectiveness, inhibited mycelial growth when used at label rates. Wettable sulphur was ineffective in both assays. On detached apple fruit, carbendazim, cyprodinil, trifloxystrobin and isopyrazam (in order of effectiveness) inhibited lesion development.

scholarly journals Evaluation of high dilutions extract of pyroligneous on the development of Colletotrichum gloeosporioides

2020 ◽  
Vol 11 (2) ◽  
pp. 69-77
Author(s):  
Cleverson Rodrigues ◽  
João Aguilar Massaroto ◽  
Grace Queiroz David Peres ◽  
Dilânia Lopes de Matos ◽  
Walmor Moya Peres ◽  
...  
Keyword(s):  
Spore Germination ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Tectona Grandis ◽  
Maximum Reduction ◽  
Randomized Design ◽  
High Dilutions ◽  
Completely Randomized Design ◽  
Mother Tincture ◽  
Mycelial Development

The aim of this research was to evaluate the fungitoxicity of high dilutions of tectonic (Tectona grandis) extract in mycelial growth, sporulation and germination of the fungus Colletotrichum gloeosporioides. In a completely randomized design with six dynamizations of the pyroligneous extract (3, 9, 15, 21, 27 and 33 CH) and three controls (distilled water, mother tincture, 30% hydroalcoholic solution) with four replicates each. All treatments were diluted to 0.1% in BDA medium. Mycelial growth rate index, relative percentage of mycelial development (PRD%), inhibition of sporulation and spore germination (%) were evaluated. The treatments did not differ among them for mycelial growth, percentage of spore germination and IVCM. The PRD and sporulation variables varied according to the dynamization, with a maximum reduction of 7% in PRD (33 CH) and a 70% increase in sporulation (3 and 21 CH). The dynamized pirolenous extract did not present expressive fungitoxicity in the growth, sporulation and germination of C. gloeosporioides.

scholarly journals Fungitoxicity of the Pyroligneous Extract in the Development in vitro of Colletotrichum gloeosporioides

2020 ◽  
Vol 8 (4) ◽  
pp. 82
Author(s):  
Cleverson Rodrigues ◽  
João Aguilar Massaroto ◽  
Grace Queiroz David ◽  
Dilânia Lopes de Matos ◽  
Walmor Moya Peres ◽  
...  
Keyword(s):  
Spore Germination ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Tectona Grandis ◽  
Growth Speed ◽  
Randomized Design ◽  
Agar Media ◽  
Completely Randomized Design ◽  
Development In Vitro

Products derived from renewable sources have been studied for their use in agriculture enabling increments in the production of agricultural culture, such as in the case of the pyroligneous extract. The research aimed at evaluating the fungitoxic potential of the pyroligneous extract of teak (Tectona grandis L.) on mycelial growth, sporulation and spore germination of Colletotrichum gloeosporioides. The experiment was conducted in completely randomized design with five treatments and four replications. Five doses of pyroligneous extract were tested (0, 25, 50, 100, 150 mL L-1) following the pour-plate methodology in 10 mL of potato dextrose agar media and kept in the incubating room maintained at 25 ± 2 °C in a 12 hour-photoperiod for eight days. Mycelial growth variables (cm), mycelial index growth speed, growth inhibition (%), sporulation inhibition (%) and spore germination (%) were evaluated. There was a reduction for the mycelial growth as well as for the rate of mycelial growth, sporulation inhibition and spore germination as the doses were increased. The percentage inhibition of mycelial growth was 56 % for the highest dose (150 mL L-1). Pyroligneous extract of teak has a direct fungitoxic action on growth, sporulation and germination in vitro of the fungus C. gloeosporioides.

scholarly journals Antifungal activity of medicinal plants, Adathoda vasica and Andrographis paniculata against Colletotrichum capsici, the chilli fruit rot pathogen

2021 ◽  
Vol 42 (6) ◽  
pp. 1461-1469
Author(s):  
K. Priya ◽  
◽  
G. Thiribhuvanamala ◽  
C. Sangeetha ◽  
A. Kamalakannan ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Spore Germination ◽  
Mycelial Growth ◽  
Andrographis Paniculata ◽  
Fruit Rot ◽  
Antimicrobial Compounds ◽  
Ftir Analysis ◽  
Antimicrobial Metabolites ◽  
Ethyl Acetate Extracts

Aim: The objective was formulated to screen the extracts of medicinal plants for tapping the antimicrobial activity against Collectotrichum capsici. Further, the work was planned to characterize and identify the nature of antimicrobial compounds and their functional groups. Methodology: Extracts of eleven medicinal plants were tested against the mycelial growth and spore germination of C. capsici under in-vitro conditions. Based on these results, the potential plant extracts of A. vasica and A. paniculata found effective against C. capsici were assayed for the presence of antimicrobial metabolites through TLC, GC-MS and FTIR analysis. Results: Among the medicinal plants screened, the crude extracts from Adathoda vasica and Andrographis paniculata inhibited mycelial growth and spore germination of C. capsici by 53.33% and 38.14%, respectively, under in-vitro conditions. GC-MS analysis of ethyl acetate extracts of A. vasica indicated antimicrobial compound, 1H-Pyrrolo[2,1-b]quinazolin-9-one,3-hydroxy-2,3-dihydro- and A. paniculata showed the presence of two compounds, docosahexaenoic acid and oleic acid. Similarly, FTIR analysis revealed esters, alcohols, and halide groups, which are known antimicrobials. Interpretation: The medicinal plants, A. paniculata and A. vasica possessed antimicrobial metabolites, which was responsible for inhibiting the mycelial growth and spore germination of C. capsici.

scholarly journals Antifungal activity of extracts from Brazilian Cerrado plants on Colletotrichum gloeosporioides and Corynespora cassiicola

2011 ◽  
Vol 13 (4) ◽  
pp. 408-412 ◽  
Author(s):  
E.S. Naruzawa ◽  
M.F.S. Papa
Keyword(s):  
Antifungal Activity ◽  
Spore Germination ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Culture Media ◽  
Growth Stimulation ◽  
Corynespora Cassiicola ◽  
Brazilian Cerrado ◽  
Leaf Extracts ◽  
Caryocar Brasiliense

This study aimed to determine the antifungal activity of leaf aqueous and hydroethanolic extracts of 10 plants from the Brazilian Cerrado on Colletotrichum gloeosporioides and Corynespora cassiicola. Antifungal activity was measured through the incorporation of each extract in a culture media or spore suspension, at 50% concentration relative to the volume, determining respectively the mycelial growth and the spore germination. Then, the percentages of mycelial growth inhibition and spore germination inhibition were obtained based on the comparison with the control. The extracts had a variable action on the phytopathogens, from mycelial growth stimulation for Aristolochia esperanzae and Byrsonima verbascifolia extracts to complete inhibition of mycelial growth and spore germination for Myracrodruon urundeuva and Lafoensia pacari extracts. M. urundeuva, L. pacari and Caryocar brasiliense leaf extracts had antifungal activity against Colletotrichum gloeosporioides and Corynespora cassiicola; the hydroethanolic extracts presented more antifungal activity than the aqueous extracts, and spore germination of both phytopathogens was more affected than their mycelial growth.

scholarly journals Antifungal Activity of Alpha-Mangostin against Colletotrichum gloeosporioides In Vitro and In Vivo

Molecules ◽  
2020 ◽  
Vol 25 (22) ◽  
pp. 5335
Author(s):  
Huochun Ye ◽  
Qin Wang ◽  
Fadi Zhu ◽  
Gang Feng ◽  
Chao Yan ◽  
...  
Keyword(s):  
Spore Germination ◽  
Inhibitory Activity ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Crop Protection ◽  
Ultrastructural Observation ◽  
Garcinia Mangostana ◽  
Mitochondrial Energy Metabolism

We investigated alpha-mangostin (α-mangostin, α-MG), a xanthone natural product extracted from the pericarp of mangosteen (Garcinia mangostana), for its antifungal activities and possible mechanism against Colletotrichum gloeosporioides, which causes mango anthracnose. The results demonstrated that α-MG had a relatively high in vitro inhibitory activity against C. gloeosporioides among 20 plant pathogenic fungi. The median effective concentration (EC50) values of α-MG against mycelial growth were nearly 10 times higher than those of spore germination inhibition for both strains of C. gloeosporioides, the carbendazim-sensitive (CBD-s) and carbendazim-resistant (CBD-r). The results suggested that α-MG exhibited a better inhibitory effect on spore germination than on the mycelial growth of C. gloeosporioides. Further investigation indicated that the protective effect could be superior to the therapeutic effect for mango leaves for scab development. The morphological observations of mycelium showed that α-MG caused the accumulation of dense bodies. Ultrastructural observation further revealed that α-MG caused a decrease in the quantity and shape of the swelling of mitochondria in the mycelium cells of C. gloeosporioides. In addition, bioassays disclosed that the inhibitory activity of α-MG on spore germination was reduced by adding exogenous adenosine triphosphate (ATP). These results suggested that the mode of action of α-MG could be involved in the destruction of mitochondrial energy metabolism. The current study supports α-MG as a natural antifungal agent in crop protection.

scholarly journals First report of Penicillium glaucum Link causing Penicillium rot of pear fruits Pyrus communis L. in Jammu and Kashmir, India

2017 ◽  
Vol 4 (8) ◽  
pp. 265-272
Author(s):  
Shazia Parveen ◽  
Abdul Hamid Wani ◽  
Mohd Yaqub Bhat ◽  
Tariq Ahmad Wani ◽  
Abdul Rashid Malik
Keyword(s):  
Spore Germination ◽  
Mycelial Growth ◽  
Pyrus Communis ◽  
Fruit Rot ◽  
Morphological Data ◽  
Morphological Observation ◽  
Pathogenicity Test ◽  
First Report ◽  
Pyrus Communis L

Pears Pyrus communis L. collected from different sites of Kashmir Valley were found infected with Penicillium glaucum causing Penicillium rot. The diseased fruits appears light tan to dark brown. The decayed tissue becomes soft, watery and the lesion has a very sharp margin between diseased and healthy tissues. Decayed fruit has an earthy, musty odor. The pathogen was isolated and cultured on PDA medium for further fungal morphological observation and confirming its pathogenicity according to Koch’s postulates. Results of morphological data and pathogenicity test showed that the pears were infected by Penicillium glaucum Link resulting in Penicillium rot of pears. To our knowledge, it is the first report of pear fruit rot caused by P. glaucum in India. Study was also undertaken for the management of Penicillium rot of pear with some fungicides. It was revealed from the study that different concentration of fungicides brought about significant reduction in the mycelial growth and spore germination of Penicillium glaucum under in vitro conditions. Amongst the tested fungicides, carbendazim proved highly effective in inhibiting the mycelial growth and spore germination of Penicillium glaucum followed by hexaconozole, bitertanol and myclobutanil respectively. Higher concentration proved effective than lower concentrations.

scholarly journals Inhibition of spore germination and mycelial growth of three fruit rot pathogens using some chemical fungicides and botanical extracts

1970 ◽  
Vol 5 ◽  
pp. 23-27 ◽  
Author(s):  
Most. Ferdousi Begum ◽  
Mst Ferdowsi Mahal ◽  
Md Shahidul Alam
Keyword(s):  
Plant Extracts ◽  
Spore Germination ◽  
Mycelial Growth ◽  
Inhibitory Effect ◽  
Fruit Rot ◽  
Inhibitory Effects ◽  
Datura Metel ◽  
Alternaria Tenuis ◽  
Chemical Fungicides ◽  
Seed Extracts

Six chemical fungicides and extracts of 15 locally available plants were tested against three fruit rot pathogens viz. Fusarium oxysporium f. sp. capsici, Rhizopus artocurpi and Alternaria tenuis for the evaluation of inhibition of spore germination and mycelial growth. Among the fungicides tested, all the concentrations of Ridomil showed 100% inhibition of spore germination and the least inhibition was recorded in case of treatment with Bavistin for F. oxysporium f. sp. capsici. In case of R. artocarpi and A. tenuis, Dithene–M 45 showed good inhibitory effects but Thiovit had no inhibitory effect against A. tenuis. Both leaf and seed extracts of Azadirachta indica showed good inhibitory effect than tested with other plant extracts. Moringa oleifera extract showed least inhibitory effect against all the fungi tested. Datura metel, Plygonum oriantale, Tagestes patula and Micania scandens also showed promising inhibition on spore germination and mycelial growth of all the pathogens tested. Key words: Fruit rot pathogens; plant extracts; chemical fungicides; spore germination; mycelial growth; PIGR DOI: 10.3329/jles.v5i0.7344 J. Life Earth Sci., Vol. 5: 23-27, 2010

Sign in / Sign up

Export Citation Format

Share Document