Comparative Studies of Remazol Brillant Blue Removal by Immobilized Organisms; Investigation of Metabolites by GC/MS and FTIR Spectrometry

2015 ◽  
Vol 98 (2) ◽  
pp. 445-449 ◽  
Author(s):  
Hatice Ardag Akdogan ◽  
Merve Canpolat Topuz
Keyword(s):  
Biological Treatment ◽  
Low Cost ◽  
White Rot Fungi ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Synthetic Dyes ◽  
Viable Option ◽  
Chemical Pollutants ◽  
Ftir Spectrometry ◽  
Textile Industries

Abstract Reactive dyes are important chemical pollutants from textile industries. Treatment of effluents from dye-based industries poses a major problem, and biotreatment with white rot fungi seems to be a viable option. The biological treatment of synthetic dyes at a low cost and in the shortest possible time is used especially in dye and textile industries and leads to pollution in the wastewater dumped into the environment by these industries. For this study, decolorization of the recalcitrant dye Remazol Brilliant Blue R by immobilized Pleurotus ostreatus and Coprinus plicatilis was investigated. This dye was removed 100% (dye concentration: 10.0 mg/L) by both immobilized organisms. Extracellular ligninolytic enzyme activities were also measured during the decolorization. There was an attempt to identify metabolites with FTIR spectrometry and GC/MS at the end of the decolorization. These results indicated that the samples did not include any detectable metabolite.

Comparison of Remazol Brillant Blue Removal from Wastewater by Two Different Organisms and Analysis of Metabolites by GC/MS

2014 ◽  
Vol 97 (5) ◽  
pp. 1416-1420 ◽  
Author(s):  
Hatice Ardag Akdogan ◽  
Merve Canpolat
Keyword(s):  
Biological Treatment ◽  
Extracellular Enzymes ◽  
Low Cost ◽  
Environmental Pollutants ◽  
White Rot ◽  
White Rot Fungus ◽  
Synthetic Dyes ◽  
Biological Degradation ◽  
Peroxidase Enzyme ◽  
Textile Industries

Abstract White rot fungus participates in biological degradation of many organic environmental pollutants. Also, white rot fungus contains a variety of extracellular enzymes, and these enzymes are used for biological degradation of organic matter. We investigated the biological treatment of synthetic dyes, at a low cost and in the shortest possible time, that are used especially in the dye and textile industries and are important polluting agents in the wastewater discharged into the environment by these industries. For this purpose, removal of Remazol Brillant Blue by Pleurotus ostreatus and Coprinus plicatilis was studied. This dye was removed 100% (dye concentration, 10.0 mg/L) by both organisms. Laccase and manganese peroxidase enzyme activities were also monitored. There was an attempt to identify metabolites via GC/MS at the end of the decolorization. No detectable metabolite was found.

scholarly journals Research on the changes of physiological characteristics of algal cells in the process of algae dissolving by immobilized white rot fungi

2020 ◽  
Vol 165 ◽  
pp. 05033
Author(s):  
Guoming Zeng ◽  
Jing Luo ◽  
Xiaowan Liu ◽  
Maolan Zhang ◽  
Hengjun Tang
Keyword(s):  
Low Cost ◽  
White Rot Fungi ◽  
Physical Method ◽  
White Rot ◽  
Ecological Security ◽  
Algal Control ◽  
The Cost ◽  
Water Eutrophication ◽  
By Products ◽  
Control Water

With the increasing of water pollution, water eutrophication is seriously affecting people’s daily life and production. Therefore, it is particularly important to explore safe and efficient algae control technology. In the current algal bloom treatment methods, the physical method is not complete in algae dissolving, and the cost of algal control is high. The chemical method is easy to produce secondary pollution and toxic by-products, and the safety is not high. However, the biological method has the advantages of low cost, high ecological security and good ecological compatibility. It is considered to be a more promising method to remove algae and biological toxins, and it is also an inevitable trend to control water eutrophication in the future.

scholarly journals Samanlarda Biyolojik Muamelelerle Lignoselüloz Kompleksin Sindirilebilirliğinin Artırılması

2017 ◽  
Vol 5 (13) ◽  
pp. 1704
Author(s):  
Aydan Atalar ◽  
Nurcan Çetinkaya
Keyword(s):  
Biological Treatment ◽  
Hydrolytic Enzymes ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Brown Rot ◽  
Soft Rot ◽  
White Rot ◽  
Enzyme Treatment ◽  
Environmental Friendliness ◽  
Brown Rot Fungi

The efforts to break down the lignocellulosic complex found in the cell wall of straws, besides digestible cellulose and hemicellulose by rumen fermentation, improvement of straw digestibility by the degradation of indigestible lignin fraction of complex by using of biotechnological methods is one of the focus areas of animal nutritionists in recent years. Biological method sare prefer redover other methods due to the environmental friendliness. In the biological treatment methods of lignocellulosic complex, biodiversity of bacteria, enzymes and fungi gives opportunity to select lignin degrading species. Mycobacterium, Arthrobacter and Flavobacterium genre bacteria are used to degrade lignin by bacterial treatment. Lignocellulolytic enzymes isolated from different varieties of fungi are used in enzyme treatment. There are 3 genres of fungus that are white, Brown and soft rot in fungal treatments. Brown rot fungi prefer ably attack cellulose and hemicelluloses, but not lignin. White rot fungi attack the lignin and break up lignol bonds and aromatic ring. White rot fungi break down polysaccharides with hydrolytic enzymes such as cellulase, xylanase, and lignin with oxidative ligninolytic enzymes such as lignin peroxidase and laccase. Because of the fact that the microorganisms that can break down the lignocellulosic materials are the fungi and the cost is low, the application of white rot fungi is possible. In this paper, improvement the lignocellulosic comlex digestibility of straw by biological treatment with the advantage of biodiversity is discussed.

scholarly journals Effect of initial fermentation medium on bioacetone, biobutanol, and bioethanol (BioABE) production from fermentable sugars of Acacia mangium using Clostridium acetobutylicum YM1

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 6912-6927
Author(s):  
Rafidah Jalil ◽  
Mohd Sahaid Kalil ◽  
Norliza Abd Rahman ◽  
Abdulalati Ibrahim Al-Tabib ◽  
Aidil Abdul Hamid ◽  
...  
Keyword(s):  
Clostridium Acetobutylicum ◽  
Low Cost ◽  
White Rot Fungi ◽  
Acacia Mangium ◽  
Product Yield ◽  
Fermentation Medium ◽  
White Rot ◽  
Fermentable Sugars ◽  
Process Variables ◽  
Initial Ph

Bioacetone, biobutanol, and bioethanol (BioABE) production is dependent on the fermentable sugars produced from lignocellulosic biomass and on the composition and initial pH of the medium. Understanding these process variables and their interconnectedness could enhance the BioABE product yield. Acacia mangium is available abundantly and it is a potential feedstock for BioABE production. In this study, BioABE was produced from fermentable sugars of A. mangium using Clostridium acetobutylicum YM1. Alkaline treated A. mangium (70 °C, 3 h, 5.50 %w/v NaOH) was further hydrolyzed via enzymatic hydrolysis using a multi-enzyme of white rot fungi to convert it into fermentable sugars. Approximately 15 g/L of fermentable sugars was produced from A. mangium (100 g/L) and was used for BioABE production in comparison with glucose. Initial findings showed that only 0.94 g/L of BioABE was produced in comparison with glucose (2.86 g/L) at a pH of 6.2. Decreasing the initial pH of the medium to 4.50 increased the BioABE (2.87 g/L), and after the medium was supplemented with tryptone-yeast-acetate (TYA), the BioABE yield increased by more than 100% to 6.84 g/L. This study discovered that BioABE produced from A. mangium was comparable to using commercial glucose, thus offering high potential as a low-cost feedstock.

scholarly journals Comparison of the Ability of Several White-rot Fungi to Biobleach Acacia Oxygen-delignified Kraft Pulp

2019 ◽  
pp. 1-10
Author(s):  
Sitompul Afrida ◽  
Toshihiro Watanabe ◽  
Yutaka Tamai
Keyword(s):  
Kraft Pulp ◽  
Extracellular Enzymes ◽  
Low Cost ◽  
White Rot Fungi ◽  
Acacia Mangium ◽  
White Rot ◽  
Irpex Lacteus ◽  
Lentinus Tigrinus

Previous screening analyses demonstrated that the in vivo biobleaching activities of the white-rot fungi Irpex lacteus KB-1.1 and Lentinus tigrinus LP-7 are higher than those of Phanerochaete chrysosporium and Trametes versicolor. The purpose of the current study was to examine the production of extracellular enzymes of these four white-rot fungi grown on three types of low-cost media containing agricultural and forestry waste, and to evaluate the ability of the produced extracellular enzymes to biobleach Acacia oxygen-delignified kraft pulp (A-OKP). The biobleaching activity of extracellular fractions of I. lacteus, L. tigrinus, T. versicolor, and P. chrysosporium cultures was the most pronounced after 3 days of incubation with Acacia mangium wood powder supplemented with rice bran and 1% glucose (WRBG) with resultant Kappa number reduction of 4.4%, 6.7%, 3.3%, and 3.3%, respectively. Therefore, biobleaching ability of I. lacteus and L. tigrinus have been shown to be higher than of T. versicolor and P. chrysosporium, both in vivo and in vitro.

Biological Pretreatment of Lignocellulosic Material for Biopulping: A Review

2016 ◽  
Vol 23 (1) ◽  
pp. 1-12
Author(s):  
Shardesh Chaurasia ◽  
Prakashchandra Mervana ◽  
Satyapal Singh ◽  
Sanjay Naithani
Keyword(s):  
Plant Cell Wall ◽  
Energy Savings ◽  
Paper Industry ◽  
White Rot Fungi ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Cellulose Fibres ◽  
Paper Properties ◽  
Environmental Threats ◽  
Almost All

Biopulping has the potential to improve pulp quality, paper properties and to reduce energy costs and environmental impact relative to traditional pulping approaches. The technology has focused on the white rot fungi that are known to be degrader of wood constituents. This group of fungi have complex extracellular ligninolytic enzyme systems that can selectively degrade/ alter lignin structure and allow cellulose fibres to be relatively unaffected. It colonizes either on living or dead wood and decomposes almost all plant cell wall polymers including lignin and extractives making it to be extremely potential to be used in biopulping. Biopulping reduces the chemical load in paper industry and thus partially limiting environmental threats caused by conventional pulping. It has been advised that energy savings alone could make the process economically viable. Other benefits include improved burst strength and tear indices of product and reduced pitch deposition.

Bioremediation of a wine distillery wastewater using white rot fungi and the subsequent production of laccase

2007 ◽  
Vol 56 (2) ◽  
pp. 179-186 ◽  
Author(s):  
P.J. Strong ◽  
J.E. Burgess
Keyword(s):  
Biological Treatment ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Total Phenolic ◽  
Pycnoporus Cinnabarinus ◽  
Distillery Wastewater

The aim of this work was to ascertain whether a submerged culture of a white rot fungus could be used to treat distillery wastewater, and whether the compounds present in the wastewater would stimulate laccase production. Trametes pubescens MB 89, Ceriporiopsissubvermispora, Pycnoporus cinnabarinus and UD4 were screened for their ability for the bioremediation of a raw, untreated distillery wastewater as well as distillery wastewater that had been pretreated by polyvinylpolypyrrolidone. Suitability of each strain was measured as a function of decreasing the chemical oxygen demand (COD) and total phenolic compounds concentration and the colour of the wastewater, while simultaneously producing laccase in high titres. After screening, T. pubescens MB 89 was used further in flask cultures and attained 79±1.1% COD removal, 80±4.6% total phenols removal, 71±1.6% decrease in colour at an absorbance of 500 nm and increased the pH from 5.3 to near-neutral. Laccase activity in flask cultures peaked at 4,644±228 units/l, while the activity in a 50 l bubble lift reactor peaked at 12,966±71 units/l. Trametes pubescens MB 89 greatly improved the quality of a wastewater known for toxicity towards biological treatment systems, while simultaneously producing an industrially relevant enzyme.

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