Diarrheagenic Escherichia Coli and Probiotic Activity against Foodborne Pathogens: A Brief Review

Diarrheagenic Escherichia coli pathotypes (DEP) are important foodborne pathogens in various countries, including Mexico. However, no data exist on the presence of DEP on fresh tomatoes (Solanum lycopericum) from Mexico. The frequency of fecal coliforms (FC), E. coli, and DEP were determined for two tomato varieties. One hundred samples of a saladette tomato variety and 100 samples of a red round tomato variety were collected from public markets in Pachuca, Mexico. Each tomato sample consisted of four whole tomatoes. For the 100 saladette samples, coliform bacterial, FC, E. coli, and DEP were identified in 100, 70, 60, and 10% of samples, respectively. For the 100 red round samples, coliform bacterial, FC, E. coli, and DEP were identified in 100, 75, 65, and 11% of samples, respectively. Identified DEP included Shiga toxin–producing E. coli (STEC), enteroinvasive E. coli (EIEC), enteropathogenic E. coli (EPEC), and enterotoxigenic E. coli (ETEC). STEC were isolated from 6% of saladette samples and 5% of red round samples. ETEC were isolated from 3% of saladette samples and 4% of red round samples. EPEC were isolated from 2% of saladette samples and 3% of red round samples, and EIEC were isolated from 1% of saladette samples. Both STEC and ETEC were identified in two saladette samples and 1 red round sample. E. coli O157:H7 was not detected in any STEC-positive samples.

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Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

Bangladesh Journal of Medical Microbiology ◽

10.3329/bjmm.v1i2.21506 ◽

2016 ◽

Vol 1 (2) ◽

pp. 38-42 ◽

Cited By ~ 4

Author(s):

Khairun Nessa ◽

Dilruba Ahmed ◽

Johirul Islam ◽

FM Lutful Kabir ◽

M Anowar Hossain

Keyword(s):

Escherichia Coli ◽

Multiplex Pcr ◽

Clinical Laboratory ◽

Proteinase K ◽

Microbiology Laboratory ◽

Pcr Assay ◽

E Coli ◽

Diarrheagenic Escherichia Coli ◽

Multiplex Pcr Assay ◽

Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

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Evaluation of an WPC edible film added with a cocktail of six lytic phages against foodborne pathogens such as enteropathogenic and Shigatoxigenic Escherichia coli

LWT ◽

10.1016/j.lwt.2019.108316 ◽

2019 ◽

Vol 113 ◽

pp. 108316 ◽

Cited By ~ 3

Author(s):

David Tomat ◽

Marina Soazo ◽

Roxana Verdini ◽

Cecilia Casabonne ◽

Virginia Aquili ◽

...

Keyword(s):

Escherichia Coli ◽

Foodborne Pathogens ◽

Edible Film

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Molecular Typing and Prevalence of Extended‐Spectrum β‐Lactamase Genes in Diarrheagenic Escherichia coli strains Isolated from Foods and Humans in Mashhad, Iran

Journal of Applied Microbiology ◽

10.1111/jam.15062 ◽

2021 ◽

Author(s):

Neda Fallah ◽

Mehrnaz Rad ◽

Kiarash Ghazvini ◽

Mehran Ghaemi ◽

Abdollah Jamshidi

Keyword(s):

Escherichia Coli ◽

Molecular Typing ◽

Diarrheagenic Escherichia Coli ◽

Extended Spectrum

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Emergence of oxacillinase-181 carbapenemase-producing diarrheagenic Escherichia coli in Ghana

Emerging Microbes & Infections ◽

10.1080/22221751.2021.1920342 ◽

2021 ◽

pp. 1-43

Author(s):

Isaac Prah ◽

Alafate Ayibieke ◽

Samiratu Mahazu ◽

Chihiro Tani Sassa ◽

Takaya Hayashi ◽

...

Keyword(s):

Escherichia Coli ◽

Diarrheagenic Escherichia Coli

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Influence of Bacterial Competitors on Salmonella enterica and Enterohemorrhagic Escherichia coli Growth in Microbiological Media and Attachment to Vegetable Seeds

Foods ◽

10.3390/foods10020285 ◽

2021 ◽

Vol 10 (2) ◽

pp. 285

Author(s):

Da Liu ◽

Ronald Walcott ◽

Kevin Mis Solval ◽

Jinru Chen

Keyword(s):

Escherichia Coli ◽

Foodborne Pathogens ◽

Salmonella Enterica ◽

Lactobacillus Rhamnosus ◽

Probiotic Bacterium ◽

Enterohemorrhagic Escherichia Coli ◽

Biocontrol Agents ◽

Human Pathogens ◽

Bacterial Strains ◽

Pathogen Populations

Interests in using biological agents for control of human pathogens on vegetable seeds are rising. This study evaluated whether probiotic bacterium Lactobacillus rhamnosus GG, bacterial strains previously used as biocontrol agents in plant science, as well as a selected plant pathogen could compete with foodborne human pathogens, such as Salmonella enterica and enterohemorrhagic Escherichia coli (EHEC), for growth in microbiological media and attachment to vegetable seeds; and to determine whether the metabolites in cell-free supernatants of competitive bacterial spent cultures could inhibit the growth of the two pathogens. The results suggest that the co-presence of competitive bacteria, especially L. rhamnosus GG, significantly (p < 0.05) inhibited the growth of Salmonella and EHEC. Cell-free supernatants of L. rhamnosus GG cultures significantly reduced the pathogen populations in microbiological media. Although not as effective as L. rhamnosus GG in inhibiting the growth of Salmonella and EHEC, the biocontrol agents were more effective in competing for attachment to vegetable seeds. The study observed the inhibition of human bacterial pathogens by competitive bacteria or their metabolites and the competitive attachment to sprout seeds among all bacteria involved. The results will help strategize interventions to produce vegetable seeds and seed sprouts free of foodborne pathogens.

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Isolation and characterization of pathogenic Escherichia coli bacteriophages from chicken and beef offal

BMC Research Notes ◽

10.1186/s13104-019-4859-y ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Celosia Lukman ◽

Christopher Yonathan ◽

Stella Magdalena ◽

Diana Elizabeth Waturangi

Keyword(s):

Escherichia Coli ◽

Foodborne Pathogens ◽

High Efficiency ◽

Multiplicity Of Infection ◽

Isolation And Characterization ◽

Transmission Electron ◽

Pathogenic Escherichia Coli ◽

Family Based

Abstract Objective This study was conducted to isolate and characterize lytic bacteriophages for pathogenic Escherichia coli from chicken and beef offal, and analyze their capability as biocontrol for several foodborne pathogens. Methods done in this research are bacteriophage isolation, purification, titer determination, application, determination of host range and minimum multiplicity of infection (miMOI), and bacteriophage morphology. Results Six bacteriophages successfully isolated from chicken and beef offal using EPEC and EHEC as host strain. Bacteriophage titers observed between 109 and 1010 PFU mL−1. CS EPEC and BL EHEC bacteriophage showed high efficiency in reduction of EPEC or EHEC contamination in meat about 99.20% and 99.04%. The lowest miMOI was 0.01 showed by CS EPEC bacteriophage. CI EPEC and BL EPEC bacteriophage suspected as Myoviridae family based on its micrograph from Transmission Electron Microscopy (TEM). Refers to their activity, bacteriophages isolated in this study have a great potential to be used as biocontrol against several foodborne pathogens.

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Autotransporter Protein-Encoding Genes of Diarrheagenic Escherichia coli Are Found in both Typical and Atypical Enteropathogenic E. coli Strains

Applied and Environmental Microbiology ◽

10.1128/aem.02635-12 ◽

2012 ◽

Vol 79 (1) ◽

pp. 411-414 ◽

Cited By ~ 21

Author(s):

Afonso G. Abreu ◽

Vanessa Bueris ◽

Tatiane M. Porangaba ◽

Marcelo P. Sircili ◽

Fernando Navarro-Garcia ◽

...

Keyword(s):

Escherichia Coli ◽

Content Type ◽

E Coli ◽

Diarrheagenic Escherichia Coli ◽

Virulence Markers ◽

Protein Encoding ◽

Encoding Genes ◽

Specific Virulence

ABSTRACTAutotransporter (AT) protein-encoding genes of diarrheagenicEscherichia coli(DEC) pathotypes (cah,eatA,ehaABCDJ,espC,espI,espP,pet,pic,sat, andtibA) were detected in typical and atypical enteropathogenicE. coli(EPEC) in frequencies between 0.8% and 39.3%. Although these ATs have been described in particular DEC pathotypes, their presence in EPEC indicates that they should not be considered specific virulence markers.

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