scholarly journals Profil Dan Sensitivitas Protein Alergen Ikan Tongkol (Thunnus albacares) Sebagai Reagen Skin Prick Test (SPT)

2019 ◽  
Vol 5 (1) ◽  
pp. 44-55
Author(s):  
Sri Yadial Chalid ◽  
Dahrul Syah ◽  
Puspo Edi Giriwono ◽  
Fransiska Rungkat ◽  
Zakaria Zakaria
Keyword(s):  
Molecular Weight ◽  
Skin Prick Test ◽  
Clinical Symptoms ◽  
Protein Profile ◽  
Thunnus Albacares ◽  
Tuna Fish ◽  
Prick Test ◽  
Allergy Test ◽  
Allergen Protein ◽  
Marine Products

Ikan tongkol merupakan salah satu produk laut penyebab alergi makanan. Gejala klinis reaksi alergi makanan adalah gatal, bentol, bengkak, sesak nafas, batuk, dan yang terparah adalah syok anafilaksis. Pengobatan dan pencegahan alergi makanan yang terbaik adalah menghindari konsumsi penyebab alergi. Menghindari konsumsi suatu makanan sebaiknya berdasarkan uji alergi seperti Skin Prick Test (SPT). SPT dilakukan menggunakan reagen SPT yang dicukitkan pada lapisan epidermis kulit lengan bagian volar. Reagen alergen pada penelitian ini merupakan protein ikan tongkol yang berasal dari laut Indonesia dan diekstrak dengan buffer fosfat, Profil ekstrak protein menggunakan elektroforesis dan immunoblotting untuk menentukan protein allergen. Sebanyak 15 pita protein terdeteksi pada ekstrak ikan tongkol yaitu protein dengan berat molekul antara 17 sampai 152 kDa. Potensi alergenik terdapat pada pita protein dengan berat molekul antara 12 sampai 50 kDa. Reagen SPT ikan tongkol mempunyai sensitivitas sebesar 81.8% dan spesifitas 100% sehingga disimpulkan bahwa reagen ekstrak ikan tongkol dapat digunakan sebagai reagen skin prick test Kata kunci: Alergi, protein, tongkol, gejala klinis, skin prick test   Tuna fish is one of the marine products that can cause allergic. Clinical symptoms of allergic are a bump, swelling, shortness of breath, coughing and anaphylactic shock is the worst symptom. The best medication and treatment is avoiding the consumption of food that could cause allergy. Avoiding consumption of food should be based on an allergy test such as a Skin Prick Test (SPT). SPT using reagent which is applied on the skin of the forearm between the wrist and elbow. The reagent for SPT is a protein solution of tuna which was extracted by phosphate buffer then protein profile was detected using electrophoresis. Immunoblotting was done to determine the molecular weight of the allergen protein. Fifteen protein bands were detected on tuna fish extract; the molecular weight of the protein was obtained between 17 to 152 kDa. Protein allergenic are molecules that have protein bands with a molecular weight between 12 and 50 kDa. Tuna fish extract solution for SPT had a sensitivity is 81.8%, and specificity is 100%, it was concluded that tuna fish reagent could be used as SPT reagent. Keywords: Allergen, protein, tuna fish, skin prick test.

Differential Correlations among Allergy Tests According to Indoor Allergens in Allergic Rhinitis

2021 ◽  
pp. 000348942110087
Author(s):  
Jae-Sung Nam ◽  
Sang Hyeon Ahn ◽  
Jong-Gyun Ha ◽  
Jeong-Jin Park ◽  
Hae Eun Noh ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Skin Prick Test ◽  
Dermatophagoides Pteronyssinus ◽  
Dermatophagoides Farinae ◽  
Indoor Allergens ◽  
Prick Test ◽  
Simultaneous Test ◽  
Allergy Test ◽  
Cat Allergen ◽  
Dog Allergen

Objectives: Several allergy tests are used for the diagnosis of allergic rhinitis; however, few studies have reported a direct comparison of the skin prick test (SPT), multiple allergen simultaneous test (MAST), and ImmunoCAP according to specific allergens. This study aimed to evaluate the correlations between each test and allergic rhinitis symptoms and to evaluate the correlations of the MAST and ImmunoCAP with the SPT for representative indoor allergens in Korea. Methods: Electronic medical charts were retrospectively reviewed, and 698 patients with allergic rhinitis who had performed SPT, MAST, and ImmunoCAP were enrolled. Correlations between each allergy test for 4 representative indoor allergens and the symptoms of allergic rhinitis were analyzed. Agreements of the MAST and ImmunoCAP with the SPT were compared according to each allergen. Results: The SPT showed higher correlations with allergic rhinitis symptoms for 4 indoor allergens ( Dermatophagoides pteronyssinus, Dermatophagoides farinae, cat, and dog allergens) than the MAST or ImmunoCAP. In comparison between the MAST and SPT, the least correlation was observed for the dog allergen, whereas between the ImmunoCAP and SPT, the least correlation was observed for the cat allergen. The correlation between the ImmunoCAP and SPT was higher than that between the MAST and SPT for the dog allergen, whereas no significant differences were noted for other allergens. Conclusions: Overall, the SPT showed a higher correlation with allergic rhinitis symptoms than the MAST or ImmunoCAP for 4 indoor allergens. ImmunoCAP showed similar reactivity to MAST; however, it showed better positivity with dog allergen in patients who were reactive to the allergen in the SPT. Care should be taken while evaluating dog allergen sensitization using the MAST.

scholarly journals Comparison of skin prick allergy test in urban and rural children

2011 ◽  
Vol 51 (2) ◽  
pp. 84
Author(s):  
Fakhri Widyanto ◽  
Rita Evalina Rusli ◽  
Muhammad Ali ◽  
Melda Deliana ◽  
Lily Irsa ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
House Dust ◽  
Urban Areas ◽  
House Dust Mites ◽  
Rural Children ◽  
Positive Skin Prick Test ◽  
Test Results ◽  
Positive Skin ◽  
Prick Test ◽  
Allergy Test

Background Children who grow up in rural areas have a lower incidence of atopy and other allergic manifestations than children in urban areas. Several recent studies have suggested that agricultural exposure may protect children from developing asthma and atopy, but these findings are inconsistent.Objective To examine an association between living in rural or urban areas and skin prick allergy test results in children and to detennine associated risk factors for atopy.Methods We conducted a cross-sectional study in Karo district (rural) and Medan (urban) in October-December 2009. We enrolled primary school children who had a history of atopy in their families. Skin prick testing was done on the volar side of the forearm and included eight aero-allergens: house dust mites, house dust, cotton, chicken feathers, cat dander, cockroaches, mould, and pollen. We analyzed the folloMng risk factors forassociation Mth atopy: tobacco smoke, pets, livestock exposure, and having older sibling(s).Results We recruited 49 children from the Karo district and 52 children from the city of Medan. There were significant associations between living in an urban area and positive skin prick test results for house dust mites and house dust compared to living in a rural area (P=0.04, 95% CI: 1.11 to 5.91; P=0.04, 95% CI: 1.13 to 12.45, respectively). The reverse was true for cockroach allergens (P=0.02, 95% CI: 0.16 to 0.81). Tobacco smoke and livestock exposure were associated Mth negative skin prick test results in rural children (P=O.03, 95% CI: 0.03 to 0.81 and P=0.002, 95% CI: 0.02 to 0.42, respectively). Multivariate analysis revealed that lack of livestock exposure was the major risk factor associated Mth any positive skin prick test results in rural children (P=0.004; 95% CI ; 0.02 to 0.49).Conclusion There were differing associations between living in rural and urban areas to various skin prick test results in children. Lack of livestock exposure was the risk factor associated with positive skin prick test results in rural children.

scholarly journals Skin Scrape Test: An Inexpensive and Painless Skin Test For Recognition Of Immediate Hypersensitivity In Children And Adults

2013 ◽  
Vol 6 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Celso Eduardo Olivier ◽  
Daiana Guedes Pinto Argentão ◽  
Raquel Acácia Pereira Goncalves dos Santos ◽  
Mariana Dias da Silva ◽  
Regiane Patussi Santos Lima ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
Human Subjects ◽  
Skin Testing ◽  
Scratch Test ◽  
Specific Ige ◽  
Prick Test ◽  
Allergy Test ◽  
Allergic Skin ◽  
Allergen Extracts ◽  
Suitable Technique

Background: Skin testing is a mainstay in allergology, and its importance is increasing in several fields. The ability to choose the most suitable technique according to the clinical setting is an advantage for the medical team. Objectives: To describe in detail an alternative technique of the coetaneous allergy test (skin scrape test) conceived as a variation of the former skin scratch test; to evaluate its value as a tool for diagnosis of immune sensitization; and to compare its accuracy with the skin prick test. Methods: The skin scrape test and skin prick test were performed side by side with the same allergen extracts in 162 human subjects classified in two groups according to the known presence or absence of serum specific-IgE to these allergens. Results: The sensitivity of the skin scrape test to detect immediate reactions was 85.0%. The sensitivity of the skin prick test was 86.5%. The sensitivity of both techniques analyzed together as a unique procedure was 94.2%. The specificity of the skin scrape test was 90.1%.The specificity of the skin prick test was 72.9%.The specificity of both tests analyzed together as a unique procedure was 70.5%. Conclusions: The skin scrape test is an alternative and complementary technique for allergic skin testing, and it is able to detect IgE-specific immune sensitization without the disadvantages of the skin scratch test. The skin scrape test has similar outcomes to the skin prick test.

scholarly journals Suitable Interpretation of Skin Prick Test and Biomedical Guidance Leads to a Better Clinical State in Atopic Individuals with High Indoor Permanence: Possible Therapeutic Implications

Biologics ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 222-230
Author(s):  
Bernardo Yassunobu Nakamatsu ◽  
Archangelo Padreca Fernandes ◽  
Débora da Silva ◽  
Ludimila Souza Santos ◽  
Thamires Rodrigues de Sousa ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
Clinical Symptoms ◽  
Specific Ige ◽  
Substantial Improvement ◽  
Clinical State ◽  
Prick Test ◽  
Therapeutic Implications ◽  
Social Habits ◽  
Indoor Conditions

Indoor conditions contribute to allergen sensitization and multiple allergens reactivity, mainly for inhaled allergens. This study analyzes if Skin Prick Test (SPT) combined with efficient individual biomedical guidance about allergy development’s social, biological, and environmental aspects can yield a better clinical state with therapeutic implications for atopic individuals with high indoor permanence. We recruited atopic and non-atopic volunteers (clinically and in vitro diagnosed) with indoor permanence above 15 h per day and without previous SPT evaluation. The SPT and serum anti-allergen IgE analyses were performed individually in person, demonstrated, and discussed by the practitioners. Six months after, SPT and specific IgE titers determination were repeated, and a questionnaire to evaluate the effectiveness of the practitioner’s orientation was performed. After six months, 14% of atopic volunteers reported changes in their social habits, 30% said that they avoid the development of allergies clinical symptoms, and 68% reported a substantial improvement in their health after being informed mentored about their allergen reactivity. The control non-atopic group, as expected, reported no changes in social habits, the maintenance of total avoidance of allergic symptoms, and almost no improvement of their health. Reduced SPT and serum allergen-specific IgE titers were detected in the atopic individuals corroborating with questionnaire results. Our results indicated that SPT, followed by an individual and efficient discussion about the main biomedical aspects of allergy development, could exert a pronounced therapeutic role in allergy development by high indoor permanence individuals.

Optimization of Cow´s Milk Skin Prick Test

2001 ◽  
Vol 13 (5) ◽  
pp. 0219-0220
Author(s):  
Cristina Di Berardino ◽  
Alfonso Angrisano ◽  
Oreste Brenna
Keyword(s):  
Skin Prick Test ◽  
Prick Test

Correlation Between Density of House Dust Mites and Recurrence of Urticaria

2019 ◽  
Vol 8 (3) ◽  
pp. 511
Author(s):  
Gardenia Akhyar ◽  
Rahma Ledika Veroci
Keyword(s):  
Skin Prick Test ◽  
House Dust ◽  
House Dust Mites ◽  
Dust Mites ◽  
Prick Test

Beberapa kasus urtikaria pada paparan debu dan diantaranya menghubungkan sensitivitas tungau debu rumah (TDR) dengan urtikaria berdasarkan riwayat pasien dan pemeriksaan uji intradermal. Tujuan penelitian ini adalah menentukan korelasi antara kepadatan TDR dan rekurensi urtikaria. Jumlah sampel pada penelitian ini 30 orang. Kepadatan TDR dari tiap kamar sampel dihitung dengan mengumpulkan debu, dan sampel dinilai sensitivitas terhadao TDR dengan pemeriksaan skin prick test (SPT). Diagnosis urtikaria berdasarkan anamnesis, kemudian ditanyaan rekurensinya dalam tiga bulan terakhir. Hasil: Dari 30 peserta, tujuh (23,3%) sampel memiliki kepadatan TDR sedang sementara 23 (76,7%) memiliki kepadatan rendah. Selain itu, 15 sampel positif SPT terhadap alergen TDR. Dari anamnesis, 12 (40%) sampel didiagnosis urtikaria dan diantaranya, 11 orang (91,67%) positif terhadap SPT. Tidak ada sampel yang memiliki rekurensi urtikaria dalam tiga bulan terakhir. Kesimpulan: Meskipun penelitian ini tidak menunjukkan korelasi yang signifikan antara kepadatan TDR dan tingkat kekambuhan urtikaria (p> 0,05), TDR memang bisa memicu eksaserbasi. Penelitian ini membutuhkan penyelidikan lebih lanjut dengan ukuran sampel yang lebih besar.

scholarly journals Skin Prick Test Predictive Values for the Outcome of Cashew Challenges in Children

2020 ◽  
Vol 8 (1) ◽  
pp. 141-148.e2 ◽  
Author(s):  
Vicki McWilliam ◽  
Rachel L. Peters ◽  
Katrina J. Allen ◽  
Shyamali C. Dharmage ◽  
Anne-Louise Ponsonby ◽  
...  
Keyword(s):  
Skin Prick Test ◽  
Predictive Values ◽  
Prick Test

scholarly journals Immunotherapy – 2066. A study on skin prick test patterns of atopic patients managed in a tertiary care setting of Sri Lanka

2013 ◽  
Vol 6 ◽  
pp. P149
Author(s):  
Anoma Siribaddana ◽  
Fariha Sitheeque ◽  
Sonali Surangi ◽  
Srinath Illeperuma ◽  
Dinesh Dassanayaka
Keyword(s):  
Sri Lanka ◽  
Skin Prick Test ◽  
Care Setting ◽  
Tertiary Care ◽  
Prick Test ◽  
Tertiary Care Setting

scholarly journals Is Atopy Related to Neuroticism, Stress, and Subjective Quality of Life?

2009 ◽  
Vol 60 (1) ◽  
pp. 99-107 ◽  
Author(s):  
Biserka Radošević-Vidaček ◽  
Adrijana Košćec ◽  
Marija Bakotić ◽  
Jelena Macan ◽  
Jasminka Bobić
Keyword(s):  
Quality Of Life ◽  
Skin Prick Test ◽  
Life Events ◽  
Stressful Life Events ◽  
Stressful Life ◽  
Positive Skin Prick Test ◽  
Subjective Quality Of Life ◽  
Positive Skin ◽  
Prick Test

Is Atopy Related to Neuroticism, Stress, and Subjective Quality of Life?Different psychosocial characteristics have been associated with allergic disorders. The aim of this study was to examine whether atopic constitution and reports of allergic symptoms were related to personality trait of neuroticism, exposure to stressful life events and estimates of quality of life. Atopy was determined by skin prick test and reports of nasal and pulmonary allergy-related symptoms. Actively working individuals of both genders took part in the study (n=145, age range: 20 to 66 years). The participants were divided in three groups. The first group was composed of those with negative skin prick test and without symptoms (n=57), the second of those with positive skin prick test but without symptoms (n=28), and the third of those with positive skin prick test and symptoms (n=60). The groups did not differ significantly in neuroticism, exposure to stressful life events, or quality of life. Women reported more pronounced neuroticism and anxiety, higher exposure to stressful life events, and were less satisfied with their environment than men. In our sample of active workers we found no association of neuroticism, exposure to stressful life events, and quality of life with atopy and allergic symptoms.

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