scholarly journals Study of Phenotypes Variability of Pollen Grains Malus domestica Borkh. by Scanning Electron Microscopy

2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Vladimíra Horčinová Sedláčková
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Malus Domestica ◽  
Pollen Grains ◽  
Malus Domestica Borkh ◽  
Scanning Electron

scholarly journals Pollen morphology of the genus Eremanthus Less. (Vernonieae, Asteraceae)

2012 ◽  
Vol 26 (1) ◽  
pp. 46-57 ◽  
Author(s):  
BenoÎt Loeuille ◽  
Raquel Maria Batista Souza-Souza ◽  
Vanessa Holanda Righetti Abreu ◽  
Cláudia Barbieri Ferreira Mendonça ◽  
Vania Gonçalves-Esteves
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Additional Data ◽  
Phylogenetic Reconstruction ◽  
Pollen Grains ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Quantitative Characters ◽  
Scanning Electron

In order to evaluate the significance of the pollen morphology for generic and infrageneric taxonomy of the genus Eremanthus (Vernonieae, Asteraceae), and to provide additional data for its phylogenetic reconstruction, the pollen of 20 of the 23 species of the genus was examined using light and scanning electron microscopy. Acetolysed pollen grains were measured, described, and illustrated using light microscopy, while non-acetolysed pollen grains were analyzed using scanning electron microscopy. Pollen grains of these species are isopolar, oblate-spheroidal in most of the species, more rarely prolate spheroidal or suboblate, subtriangular amb, tricolporate and subechinolophate. The variation among quantitative characters does not correlate with the macromorphological subdivision of the genus or with the generic or specific limits.

scholarly journals Pollen morphology of Ukrainian species of the genus Pedicularis L. (Orobanchaceae Vent.)

2012 ◽  
Vol 24 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Olena M. Peregrym ◽  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Taxonomic Revision ◽  
Palynological Data ◽  
First Time ◽  
Scanning Electron

Abstract Pollen grains of 10 species of Pedicularis occurring in Ukraine were investigated using light and scanning electron microscopy. Palynological data on 6 species are reported for the first time. General pollen morphology of the genus Pedicularis is presented. Three types of apertures (2-syncolpate, 3-colpate, and 3-syncolpate) and seven subtypes of sculpture (3-syncolpate pilate, 3-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate, 2-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate-tubeculate-perforate, 2-syncolpate microfoveolate, and 2-syncolpate microscabrate-perforate) are indentified. The subgenus Pedicularis is heterogeneous in its types of apertures and sculpture of the surface, which indicates the need of further taxonomic revision of the group.

scholarly journals Pollen morphology of the Balkan-Carpathian endemic Campanula lingulata Waldst. & Kit. (Campanulaceae)

2016 ◽  
pp. 75-82
Author(s):  
Marina Macukanovic-Jocic ◽  
Snezana Jaric
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Endemic Species ◽  
Pollen Morphology ◽  
Average Length ◽  
Pollen Grains ◽  
Polar Axis ◽  
First Time ◽  
Scanning Electron

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.

scholarly journals ELECTRON MICROSCOPIC STUDIES OF SOME ANTICANCER PLANTS POLLEN GRAINS

2021 ◽  
Vol 9 (10) ◽  
pp. 1386-1393
Author(s):  
Jayshree Sandesh Thaware ◽  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Morphological Characters ◽  
Electron Microscopic ◽  
Plant Parts ◽  
Morphological Studies ◽  
Datura Metel ◽  
Scanning Electron

Pollen is appropriately referred by some as Golden dust extremely valuable on account of their tremendous applications in science, industries and public health. No other plant part even though extremely tiny in size is packed with so much information and power. Similar to other plant parts, pollen characters are so varied that the classification system of plants can be built up entirely on the basis of pollen morphology.Palynology is the distinct branch of biology that deals with the dispersed microscopic tiny living and fossil entities including pollen grains, spores, algal and fungal fragments and others. An important aspect of Palynology is the Pollen morphology. The importance of Palynology in taxonomic and phylogenetic consideration of plants is well known. The changes occurring through hybridization and years of cultivation are reflected in pollen morphology. The scope and interest in the study of pollen morphology have widened with the advent of Scanning Electron Microscopy (SEM) and with regards to unipalynous taxa particularly the understanding of finer morphology is of fundamental importance. SEM gives a correct understanding of exine surface as the electron photographs of the surface replica of the exine provides the exact picture of the ornamentation pattern. The variation in the pollen morphological characters helps in the classification of plant taxa and their assessment of their phylogenetic relationship. In the present investigation, the pollen morphological studies were carried out of some ethnomedicinal plants like Catharanthus roseus, Allamanda cathartica, Datura metel, Brassica juncea, Raphanus sativus and Cleome viscosa pollen grains by Scanning electron microscopy. All that they possess anticancer characteristics in common.

Pollen morphology of Sabinaria magnifica (Cryosophileae, Coryphoideae, Arecaceae)

Phytotaxa ◽  
2015 ◽  
Vol 207 (1) ◽  
pp. 135 ◽  
Author(s):  
Giovanni Raul Bogota ◽  
Carina Hoorn ◽  
Wim Star ◽  
Rob Langelaan ◽  
Hannah Banks ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
The Other ◽  
Transmission Electron ◽  
Palm Species ◽  
Scanning Electron

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera. 

Palynological analysis of five selected Onosma taxa

Biologia ◽  
2008 ◽  
Vol 63 (2) ◽  
Author(s):  
Filippo Maggi ◽  
Vladislav Kolarčik ◽  
Pavol Mártonfi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Chromosome Number ◽  
Pollen Grains ◽  
Pollen Size ◽  
Palynological Analysis ◽  
Positive Correlation ◽  
Pollen Characters ◽  
Scanning Electron

AbstractPollen size and exine structure of the following five Onosma L. species from Slovakia and Italy were studied by light and scanning electron microscopy: Onosma echioides, O. tornensis, O. visianii, O. arenaria, and O. pseudoarenaria. Among the taxa studied, O. echioides has the smallest pollen grains in average (10.5–17.5 × 8.0–14.5 µm), and O. pseudoarenaria the largest grains in average (15.3–20.5 × 12.3–16.3 µm). Pollen grains of all 5 species are small sized, 3-syncolporate, subprolate, heteropolar, with ovate equatorial outlines and circular to rounded triangular polar outlines; the tectum is microechinate. A positive correlation is found between pollen size and chromosome number. The value of pollen characters for taxonomic purposes and the position of the taxa studied within the genus Onosma are discussed.

Contribution to the pollen morphology of Tragopogon (Asteraceae) in Turkey

Phytotaxa ◽  
2018 ◽  
Vol 361 (2) ◽  
pp. 168 ◽  
Author(s):  
MUTLU GÜLTEPE ◽  
SERDAR MAKBUL ◽  
SEDA OKUR ◽  
KAMİL COŞKUNÇELEBİ
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Principal Component ◽  
Numerical Analyses ◽  
Oblate Spheroidal ◽  
Pore Length ◽  
Principal Component Analyses ◽  
Scanning Electron

The pollen morphology of 25 Tragopogon L. taxa (including four subspecies and four varieties) distributed in Turkey was studied under light and scanning electron microscopy. It was observed that pollen grains of the examined Tragopogon taxa are suboblate and oblate-spheroidal in shape, and 3-zonocolpororate with fifteen lacunae. The lenghts of the polar axes and equatorial axes range from 31.57 to 40.35 µm and 35.32 to 44.65 µm, respectively. Numerical analyses show that the length of equatorial axis and the pore length are the most valuable characters among nineteen palynological traits for separating the examined taxa. The general palynological characteristics of the examined taxa allow some taxonomical evaluations for the genus. However, the results of cluster and principal component analyses did not support the division of the genus at any subgeneric level; neither did the ligule colour, as reported in the literature.

Pollen morphology of Ginkgo biloba and Cycas revoluta

1986 ◽  
Vol 64 (12) ◽  
pp. 3075-3078 ◽  
Author(s):  
N. Sahashi ◽  
J. Ueno
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ginkgo Biloba ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Spherical Form ◽  
Thin Sections ◽  
Morphological Studies ◽  
Cycas Revoluta ◽  
Scanning Electron

Morphological studies on pollen grains of Ginkgo biloba L. and Cycas revoluta Thunb. were carried out by scanning electron microscopy. The pollen grains of both species are generally oblong with 1-sulcate apertures which are shrunken as a result of dryness. However, the swollen grains show an almost spherical form with a large and rounded germinal aperture. This aperture may not correspond to any aperture type so far known, although the term "anaporate" can be fitted to the swollen pollen grains. Auricular projections, which may be derived from protrusions of the ectosexine, can be seen sometimes on the surface of the pollen grains. These projections remind us of degraded versions of the bladders that may have been present on the pollen grains of the fossil ancestor. The inner side of the exine, which can be seen in thin sections obtained with the freezing microtome, is ornamented with reticulumlike sculptures. These endosculptures may be the first reported among gymnosperm pollen grains.

scholarly journals Palynological studies on some species of Anabasis L. (Amaranthaceae) from Iran

2016 ◽  
Vol 23 (2) ◽  
pp. 247-253 ◽  
Author(s):  
M. Assadi ◽  
S.M.M. Hamdi ◽  
F. Fajani
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Species Delimitation ◽  
Pollen Grains ◽  
Pollen Characters ◽  
Plant Taxon ◽  
Scanning Electron

The paper presents pollen micromorphology by scanning electron microscopy of seven Anabasis L. species namely, A. haussknechtii Bge., A. aphylla L., A. calcarea (Charif & Allen) Bokhari & Wendelbo, A. eugeniae Iljin, A. eriopoda (Shrernk) Volkens, A. annua Bge. and A. setifera Moq. from Iran. Pollen grains in all studied species are peripolyporate, spherical and are of two basic types based on pores diameter inner of holes. The study showed that the sculpturing of exine provides valuable characters in species delimitation, sometimes even for closely related ones. An artificial key based on pollen characters for seven species is provided.Bangladesh J. Plant Taxon. 23(2): 247-253, 2016 (December)

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