scholarly journals The potential role of mesenchymal stem cells in a hypoxia model induced by sodium nitrite in testes of male albino rats

2017 ◽  
Vol 4 (02) ◽  
pp. 1128
Author(s):  
Nadia H. Ismaeil ◽  
Amany A. Osman ◽  
Elham H.A. Ali ◽  
Laila A. Rashed ◽  
Manal A. Saleh
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Sodium Nitrite ◽  
Recovery Period ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Blood Capillaries ◽  
Histopathological Alterations

Introduction: The present work aims to examine the possible role of stem cells on biochemical markers and histopathological alterations of hypoxia caused by sodium nitrite (NaNO2) toxicity in testes of male rats. Methods: In this study, 96 adult male albino rats were divided into 6 groups (16 rats each). Group 1 (G1) was the control group and received distilled H2O. Group 2 (G2) received daily NaNO2 (35 m/kg bwt/ day) via subcutaneous injection for 3 weeks. Group 3 (G3) received NaNO2 for 2 weeks and were then injected once with 2*106 mesenchymal stem cells (MSCs) intravenously and sacrificed 4 weeks later. Group 4 (G4) received NaNO2 for 2 weeks and were then injected with 2*106 MSCs followed by daily NaNO2 injection for 1 week; rats in G4 were sacrificed 4 weeks from MSCs treatment. Group 5 (G5) rats were treated with NaNO2 for 2 weeks and then left to recover for 4 weeks. Finally, Group 6 (G6) rats were treated with NaNO2 for 3 weeks and left to recover for 3 weeks, after which point they were sacrificed. Results: The results showed that NaNO2 caused oxidative damage and histopathological alterations in the rat testes, as well as increased the levels of testes malondialdehyde (MDA), nitric oxide (NO) and DNA fragmentation percentage (DNA F %). Moreover, NaNO2 decreased the elevated activities of testes catalase (CAT) and total antioxidant activity (TAA), in comparison to the control group. The histological results illustrated different distortions, vacuolization and lipid accumulations in interlobular space as well as diminution of inter cellular germ cell layers, absence of Leydig cells, irregular basement membrane of tubule, and separation within spermatogenic cells. In addition, congestion and dilation of intertubular and peripheral blood capillaries were found. Nevertheless, the administration of stem cells reduced the danger actions of sodium nitrite by enhancing biochemical marker concentration. Conclusion: There was an improvement in the histology of the rat testes, including a relatively normal order in the different stages of spermatogonia and loss of different stages of spermatocytes. Regarding the recovery period, there was also a significant improvement in each of the biochemical parameters assessed and in the histological lesions.

scholarly journals The Possible Role of Mesenchymal Stem Cells Therapy in the Repair of Experimentally Induced Colitis in Male Albino Rats

2013 ◽  
Vol 6 (2) ◽  
pp. 92-103 ◽  
Author(s):  
Sohair Ahmed Fawzy ◽  
Rahma Kamal El-din Abo-Elnou ◽  
Dalia Fathy Abd-El-Maksoud El-Deeb ◽  
Marwa Mohamed Yousry Abd-Elkader
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Albino Rats ◽  
Stem Cells Therapy ◽  
Experimentally Induced

scholarly journals Nanocurcumin Improves the Therapeutic Role of Mesenchymal Stem Cells in Liver Fibrosis Rats

2021 ◽  
Vol 11 (6) ◽  
pp. 14463-14479
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Liver Fibrosis ◽  
Therapeutic Agent ◽  
Albino Rats ◽  
Therapeutic Role ◽  
Recovery Group ◽  
Wistar Albino Rats ◽  
Promising Therapeutic Agent

Nano-curcumin (Nano-Cur) is a promising therapeutic agent that has a wide array of effective medicinal potentials. Therefore, the present inquiry aimed to assess Nano-Cur's impact on the therapeutic effect of bone-marrow-derived mesenchymal stem cells (BM-MSCs) in the rat model of liver fibrosis prompted by carbon tetrachloride (CCl4). Liver fibrosis was developed in 30male Wistar albino rats which were divided into five groups, six animals each. The 1st group (CCl4 group) was sacrificed immediately after the induction of liver fibrosis. The 2nd group received a single iv injection of BM-MSCs and left for 4weeks, the3rd group received 100mg/kg b.w. Nano-Cur 3times/week for 4weeks, the 4th group received a single iv injection of 107 BM-MSCs accompanied with Nano-Cur 3times/week for 4weeks, and the 5th group left for 4weeks without any intervention. Data revealed that treatment with BM-MSCs plus Nano-Cur alleviated liver fibrosis through reducing liver oxidative stress and restoring both liver histological picture and enzymatic profile. Additionally, companied treatment resulted in reducing TGFβ1 levels and attenuating the expression of Smad 2,3 and collagen I, III genes. Conversely, most of the pathological lesions were still detected in the recovery group. Nano-Cur improves the therapeutic role of BM-MSCs in liver fibrosis rats.

scholarly journals The Role of Mesenchymal Stem Cells in Decreasing Interleukin-12 Human Systemic Lupus Erythematosus

2020 ◽  
Vol 8 (A) ◽  
pp. 787-792
Author(s):  
Delfitri Munir ◽  
Rodiah Rahmawaty Lubis ◽  
Dewi Masyithah Darlan ◽  
Agung Putra ◽  
Iffan Allif
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Lupus Erythematosus ◽  
Mononuclear Cells ◽  
Interleukin 12 ◽  
Control Group ◽  
Systemic Lupus ◽  
Control Group Design

BACKGROUND: Systemic lupus erythematosus (SLE) disease is characterized by a loss of self-tolerance leading to a local tissue inflammation up to a massive systemic organ-spesific inflammation. Mesenchymal stem cells (MSCs) present immunomodulatory properties to control the over-activating immune responses in SLE through several mechanisms. However, the capability of MSCs to decrease interleukin (IL)-12 production in in vitro remains unclear. AIM: The aim of this study was to investigate the role of MSCs in decreasing the level of IL-12 derived from peripheral blood mononuclear cells (PBMCs) of SLE patients. METHODS: This study used a post-test control group design using a coculture of PBMCs from SLE and healthy patients with MSCs as the subjects. This study included five groups: sham (Sh), control (C), and treatment groups (T) treated by a co-culture MSCs with PBMCs at ratio dose of 1:1 (T1), 1:25 (T2), and 1:50 (T3), respectively, for 72 hours of incubation. The IL-12 levels was analysed by cytometric bead array (CBA) of flow cytometry. RESULTS: This study showed a significant decrease of IL-12 levels (p < 0.05) in T1 and T2 after 72 hours incubation of co-culture MSCs with PBMCs from SLE patient. CONCLUSION: MSCs could decrease the level of IL-12 in PBMCs of human SLE to control the inflammation of SLE disease.

The possible curative role of bone marrow-derived mesenchymal stem cells in bleomycin-induced skin changes in adult male albino rats

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Amal Abd-Elhafez ◽  
Maram Elkelany ◽  
Amany Mousa ◽  
Thorya El-deeb ◽  
Amira Kassab
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adult Male ◽  
Albino Rats ◽  
Skin Changes

Structural and chemical role of mesenchymal stem cells and resveratrol in regulation of apoptotic -induced genes in Bisphenol-A induced uterine damage in adult female albino rats

2021 ◽  
Vol 70 ◽  
pp. 101502
Author(s):  
Hanan Fouad ◽  
Eman Mohamed Faruk ◽  
Wardah Abdullah Alasmari ◽  
Eman Hassan Nadwa ◽  
Usama Fouad Ahmed Ebrahim
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bisphenol A ◽  
Adult Female ◽  
Albino Rats ◽  
Induced Genes
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