scholarly journals Content and location of lymphocyte subpopulations with markers CD4+, CD8+ and CD20+ in the esophageal tonsil of chickens and the Meckel diverticulum of ducks

2021 ◽  
Vol 12 (3) ◽  
pp. 396-402
Author(s):  
V. Т. Khomich ◽  
N. V. Dyshliuk ◽  
T. A. Mazurkevych ◽  
S. V. Guralskа ◽  
S. І. Usenko
Keyword(s):  
Immune System ◽  
Lymphoid Tissue ◽  
Meckel’S Diverticulum ◽  
Lymphocyte Subpopulations ◽  
The Body ◽  
Meckel's Diverticulum ◽  
Meckel Diverticulum ◽  
Statistical Research ◽  
Educational Work ◽  
Lymphoid Nodules

Immune formations of birds' digestive organs, including the esophageal tonsil and Meckel’s diverticulum, protect the body from foreign antigens that enter the body with food and water and play an important role in maintaining the genetic constancy of its internal environment. This unique property of the immune system is formed during ontogenesis and is associated with maintaining the selection of lymphocyte clones that are able to respond to foreign antigens and carry out a specific immune response of two types: humoral and cellular. This article presents the results of a study of T- and B-lymphocyte subpopulations of the esophageal tonsil of Shever 579 cross chickens at the age of 25, 180 and 300 days, the Meckel diverticulum of the Blagovarsky cross ducks at the age of 30, 150 and 180 days. Immunohistochemical and statistical research methods were used to determine the localization and quantitative parameters of cell populations of lymphocytes (CD4+, CD8+, CD20+) using monoclonal antibodies and the DAKO EnVision FLEX+ imaging system (Dako Cytomation, Denmark). Separate subpopulations of T-lymphocytes (CD4+ - helpers, CD8+ -cytotoxic / T-suppressors) and mature B-lymphocytes (CD20+) were found in the esophageal tonsil and Meckel diverticulum of birds. Their presence confirms that antigen-independent proliferation and differentiation of lymphocytes into effector cells occur in the immune formations of the digestive system. The lymphoid tissue of these formations is represented mainly by a well-defined diffuse form and nodules with light centers (secondary). In the esophageal tonsil of chickens, these structures are located in the tunica mucosa and tela submucosa, and in the Meckel diverticulum of ducks – also in the tunica muscularis. The content of lymphocytes with these markers predominates in diffuse lymphoid tissue compared to that in secondary lymphoid nodules. In the diffuse lymphoid tissue of the esophageal tonsil, lymphocytes are located mainly near the adenomeres and excretory ducts of the esophageal glands, blood vessels, and under the surface epithelium, and in Meckel’s diverticulum – around the crypts, in their epithelium and in the epithelium of the villi. They are also found in the light centers of lymphoid nodules and on their periphery. The indices of the content of lymphocytes with the indicated markers in the esophageal tonsil and Meckel diverticulum which we determined were associated with age characteristics of the poultry in the postnatal period of ontogenesis. According to our observations, the content of CD20+ lymphocytes was the highest, while the populations of CD4+ and CD8+ lymphocytes were much smaller. This indicates an increase in the activity and predominance of the humoral immunity over the cellular one. The content of CD20+ lymphocytes was highest in birds at the age of 180 days, that is, during their sexual maturity. The data presented in the work can be used by morphologists researching the organs of the immune system, immunologists, poultry specialists involved in breeding, using and raising poultry and in educational work.

scholarly journals Особливості локалізації лімфоїдної тканини в імунних утвореннях стінки кишечнику, дивертикулі меккеля і сліпокишкових дивертикулах качок

2017 ◽  
Vol 19 (82) ◽  
pp. 30-35
Author(s):  
T.A. Mazurkevych ◽  
V.T. Khomych
Keyword(s):  
Lymphoid Tissue ◽  
Meckel’S Diverticulum ◽  
Postnatal Period ◽  
The Body ◽  
Meckel's Diverticulum ◽  
Peyer's Patches ◽  
Peyer’S Patches ◽  
Meckel Diverticulum ◽  
Digestive Organs ◽  
The One

Mucous associated lymphoid tissue has a special place in the immune system, forming the first protective barrier against antigens that enter the body with food and air. In birds 70% of lymphoid tissue that forms the parenchyma of peripheral immune organs localized in the mucosa of tubular digestive organs. Recently, in the literature there have been reports that lymphoid tissue in tubular digestive organs of waterfowl (geese, musk ducks) can not be localized only in the mucosa, but also in muscularis. In this context, the aim of the study was to determine the features of lymphoid tissue localization in immune formations (Peyer’s patches) of the intestine wall of ducks, Meckel diverticulum and apical diverticula in postnatal period ontogenesis. Established that lymphoid tissue in Peyer’s patches of duck intestine, Meckel’s diverticulum and apical diverticula located in the mucosa and muscularis of the wall. From the one-day age of duck, the lymphoid tissue is found in the mucosa of all investigated structures and in the muscularis of the apical diverticula, and in the muscularis of Peyer’s patches of the intestine and the Meckel’s diverticulum – from 10–20-days of age.The complete morphofunctional maturity of the lymphoid tissue of mucosa Peyer's patches of intestine is reached in the 15 days-age of duck, the lymphoid tissue of the mucosa of the Meckel diverticulum – in 20-day age, and in the apical diverticula – in 10-day age. The complete morphofunctional maturity of the lymphoid tissue of muscularis Peyer's patches of intestine is reached in the 15–25 days-age of duck, the lymphoid tissue of the muscularis of the Meckel diverticulum – in 25-day age, and in the apical diverticula – in 10-day age.Area of lymphoid tissue in the mucosa of duodenal and ileum Peyer’s patches increased to 210-day-old ducks and jejunum – to 150-day-old age. Lymphoid tissue occupies the largest area in 150-day ducks in the muscularis of these structures. Area of lymphoid tissue in the mucosa of cecal Peyer’s patches increased to 90-day-old ducks, and in the muscularis – to 240-day-old age.Lymphoid tissue in the mucosa of Meckel’s diverticulum takes up the largest area in 20-day-old ducks, in apical diverticula – in 180-day-old. In the muscularis of these structures, lymphoid tissue occupies the largest area in 150-day ducks. 

scholarly journals Antibiotics and baby’s bugs — what effects have antibiotics on the bacteria present in the infant gut?

2012 ◽  
pp. 19-25
Author(s):  
Fiona Fouhy
Keyword(s):  
Immune System ◽  
Lymphoid Tissue ◽  
Gut Bacteria ◽  
Human Cells ◽  
The Body ◽  
Daily Functioning ◽  
Bacterial Cells ◽  
Beneficial Bacteria ◽  
Human Gut ◽  
Different Types

Take a moment to consider that there are ten times more bacteria present in the human gut than there are human cells in the body. Surprising and shocking as this may be, it should also occur to you that such vast numbers of bacteria are not there just by chance. In fact, these populations play numerous vital roles in our health and daily functioning. There are at least 100 trillion bacterial cells in the human gut, comprising over 500 different types, and these bacteria are involved in diverse and vital roles such as the digestion of foods, including foods which we would otherwise be unable to metabolise due to a lack of appropriate enzymes. These gut bacteria also contribute to the development of the gut-associated lymphoid tissue (GALT; part of the immune system located in the gut which is vital for developing tolerance to beneficial bacteria). Additionally, these gut bacteria ...

scholarly journals Meckel's diverticulum and the introduction of the intestines ''

2021 ◽  
Vol 43 (6) ◽  
pp. 57-58
Author(s):  
X. I. Feldman
Keyword(s):  
Celiac Disease ◽  
Intestinal Obstruction ◽  
Meckel’S Diverticulum ◽  
Abdominal Cavity ◽  
Meckel's Diverticulum ◽  
Intestinal Bleeding ◽  
Meckel Diverticulum

Meckel's diverticulum, according to various statistics, is found in cases of celiac disease in approximately 0.1-1.0% of patients. PN Yurovskaya observed 14 cases of Meckel diverticulum among 1126 patients who underwent laparotomy. During gluttony, the specified diverticulum comes across not only as an accidental find, but often turns out to be the cause of catastrophes in the abdominal cavity: intestinal obstruction, peritonitis, intestinal bleeding, etc. T. Bachinskaya and others).

scholarly journals The immune system as a biomonitor: explorations in innate and adaptive immunity

2013 ◽  
Vol 3 (2) ◽  
pp. 20120099 ◽  
Author(s):  
Niclas Thomas ◽  
James Heather ◽  
Gabriel Pollara ◽  
Nandi Simpson ◽  
Theres Matjeka ◽  
...  
Keyword(s):  
Immune System ◽  
Immune Responses ◽  
Lymphoid Tissue ◽  
Tissue Injury ◽  
Great Sensitivity ◽  
The Body ◽  
Human Immune System ◽  
Ligand Interactions ◽  
Human Immune ◽  
Body Self

The human immune system has a highly complex, multi-layered structure which has evolved to detect and respond to changes in the internal microenvironment of the body. Recognition occurs at the molecular or submolecular scale, via classical reversible receptor–ligand interactions, and can lead to a response with great sensitivity and speed. Remarkably, recognition is coupled to memory, such that responses are modulated by events which occurred years or even decades before. Although the immune system in general responds differently and more vigorously to stimuli entering the body from the outside (e.g. infections), this is an emergent property of the system: many of the recognition molecules themselves have no inherent bias towards external stimuli (non-self) but also bind targets found within the body (self). It is quite clear that the immune response registers pathophysiological changes in general. Cancer, wounding and chronic tissue injury are some obvious examples. Against this background, the immune system ‘state’ tracks the internal processes of the body, and is likely to encode information regarding both current and past disease processes. Moreover, the distributed nature of most immune responses (e.g. typically involving lymphoid tissue, non-lymphoid tissue, bone marrow, blood, extracellular interstitial spaces, etc.) means that many of the changes associated with immune responses are manifested systemically, and specifically can be detected in blood. This provides a very convenient route to sampling immune cells. We consider two different and complementary ways of querying the human immune ‘state’ using high-dimensional genomic screening methodologies, and discuss the potentials of these approaches and some of the technological and computational challenges to be overcome.

scholarly journals Vólvulo y necrosis de divertículo de Meckel gigante: reporte de un caso. [Title: Volvulus and necrosis of giant Meckel's diverticulum: a case report]

2020 ◽  
Vol 40 (1) ◽  
Author(s):  
Germán - Brito Sosa ◽  
Ana María Iraizoz Barrios
Keyword(s):  
Abdominal Pain ◽  
Case Report ◽  
Acute Appendicitis ◽  
Intestinal Obstruction ◽  
Terminal Ileum ◽  
Meckel’S Diverticulum ◽  
Exploratory Laparotomy ◽  
Meckel's Diverticulum ◽  
Meckel Diverticulum

<p><strong>Resumen</strong></p><p>El divertículo de Meckel complicado es poco frecuente en el adulto. Presentamos un paciente de 19 años de edad, con dolor abdominal de 11 horas de evolución, encontrando al realizar la laparotomía exploradora un divertículo de Meckel gigante, necrosado y volvulado. Las presentaciones más frecuentes del divertículo de Meckel son la obstrucción intestinal y la diverticulitis, esta última da una sintomatología similar a la apendicitis aguda,por lo que al realizar la exploración quirúrgica y encontrar el apéndice cecal normal, se debe visualizar los últimos 180 cm de íleo terminal.</p><p><strong>Abstract</strong><br /> Meckel's diverticulum is rare in adults. We present a 19-year-old patient with abdominal pain of 11 hours of evolution, finding a giant Meckel diverticulum, necrotic and volvulated, when performing the exploratory laparotomy. The most frequent presentations of Meckel's diverticulum are intestinal obstruction and diverticulitis, the latter being a symptomatology similar to acute appendicitis, surgical surgery and the normal cecal appendix, the last 180 cm of terminal ileum should be visualized.<strong></strong></p>

scholarly journals Морфогенез сліпокишкових дивертикулів качок віком 150–240 діб

2017 ◽  
Vol 19 (77) ◽  
pp. 96-99
Author(s):  
T.A. Mazurkevych
Keyword(s):  
Immune System ◽  
Lymphoid Tissue ◽  
Age Groups ◽  
Microscopic Structure ◽  
Maximum Thickness ◽  
Morphological Studies ◽  
Functional Features ◽  
Tunica Muscularis ◽  
The Right ◽  
Lymphoid Nodules

According to modern data, the immune system includes central and peripheral organs of immunogenesis. The latter includes the lymphoid tissue associated with the tunica mucosa of the digestive canal. Among organs of the birds digestive tract, immune formations are extremely well developed in the cecum, due to their functional characteristics. The lymphoid tissue of the cecum and their diverticula in ducks insufficiently studied. The cecal (apical) diverticulum is a cone-shaped end of the cecum, in the wall of which there is a significant amount of lymphoid tissue. The aim of the research was to study the morphogenesis of cecal diverticula in ducks at the age of 150–240 days. To achieve this goal, linear parameters (length and maximum thickness) of the diverticula of the right and left ceca were determined, the microscopic structure of the diverticulum wall and the content of lymphoid tissue in it were investigated, and determined the forms that represented lymphoid tissue in the diverticulum wall tunics. Accepted methods of morphological studies were used to perform the work. The linear parameters (length and maximum thickness) of the diverticula of the right and left ceca change in ducks between the ages of 150 and 240 days. The lymphoid tissue, which determines the functional features of the cecal diverticula, is determined in their tunica mucosa and tunica muscularis in all age groups of ducks. The content of lymphoid tissue decreases with increasing age of the bird: in the tunica mucosa – from 71.80±1.11% in 150-day-old ducks to 59.30 ± 4.04% in 240-day-old, in tunica muscularis – from 60.42 ± 1.68% for a 150-day-old bird to 30.54 ± 1.60% for a 240-day-old. In ducks aged from 150 to 210 days in the tunica mucosa of the diverticula of the right and left ceca, lymphoid tissue is represented by two structural forms: diffuse and secondary lymphoid nodules, and in 240-day-old – only diffuse lymphoid tissue. Lymphoid tissue is represented only by secondary lymphoid nodules in the tunica muscularis of the diverticula of the right and left ceca of all studied age groups of ducks.

scholarly journals An Overview of the Immunological Defenses in Fish Skin

2012 ◽  
Vol 2012 ◽  
pp. 1-29 ◽  
Author(s):  
María Ángeles Esteban
Keyword(s):  
Immune System ◽  
Lymphoid Tissue ◽  
Health Management ◽  
Current Knowledge ◽  
The Body ◽  
Mucosal Immune System ◽  
Evolutionary Development ◽  
Anatomical Location ◽  
Management Tools ◽  
Vertebrate Immune System

The vertebrate immune system is comprised of numerous distinct and interdependent components. Every component has its own inherent protective value, and the final combination of them is likely to be related to an animal’s immunological history and evolutionary development. Vertebrate immune system consists of both systemic and mucosal immune compartments, but it is the mucosal immune system which protects the body from the first encounter of pathogens. According to anatomical location, the mucosa-associated lymphoid tissue, in teleost fish is subdivided into gut-, skin-, and gill-associated lymphoid tissue and most available studies focus on gut. The purpose of this paper is to summarise the current knowledge of the immunological defences present in skin mucosa as a very important part of the fish immune system, serving as an anatomical and physiological barrier against external hazards. Interest in defence mechanism of fish arises from a need to develop health management tools to support a growing finfish aquaculture industry, while at the same time addressing questions concerning origins and evolution of immunity in vertebrates. Increased knowledge of fish mucosal immune system will facilitate the development of novel vaccination strategies in fish.

scholarly journals Dynamics of quantitative changes of diffuse lymphoid tissue cells of bronchi and lungs of guinea pigs sensitized with ovalbumin

Morphologia ◽  
2021 ◽  
Vol 15 (2) ◽  
pp. 53-58
Author(s):  
S.S. Popko ◽  
V.M. Yevtushenko
Keyword(s):  
Immune System ◽  
Respiratory System ◽  
Guinea Pigs ◽  
Lymphoid Tissue ◽  
Plasma Cells ◽  
Early Period ◽  
Allergic Inflammation ◽  
The Body ◽  
Local Immunity ◽  
Experimental Group

Background. There is a progressive increase of respiratory allergic diseases nowadays in the world, made it necessary to study the participation of the components of the immune system in these processes. From the point of view of modern aspects about the organs of the immune system, interesting are the patterns of morphogenesis and function of bronchus associated lymphoid tissue, occupied a special place in the immunological protection of the body due to the large area of contact with various antigens. Morphogenesis and reactive changes in the local immunity in respiratory system in conditions of allergic inflammation remain urgent issue of modern morphology. Objective. To study the changes in diffuse lymphoid tissue of bronchi and lungs of guinea pigs sensitized with ovalbumin. Methods. We have studied the lung of 48 guinea pigs, using histological, immunohistochemical, morphometric, statistical methods, under conditions of experimental ovalbumin-induced allergic inflammation, assessed the average number of lymphocytes, macrophages and plasma cells in the diffuse lymphoid tissue. Results. The average number of lymphocytes in diffuse lymphoid tissue of bronchi and lungs increased from the 23rd day of observation and remained at a high level until the end of the experiment, the maximum was during the early period of the development of allergic inflammation, the increasing coefficient was 4.7. The average number of plasma cells also acquired maximum elevation in the early period of allergic process, the increasing coefficient was 2.0. The most significant average number of macrophages was on the 23rd day of observation with same increasing coefficient. Among all types of immunocompetent cells of diffuse lymphoid tissue in bronchi and lungs, T-lymphocytes prevailed during the experiment elevated almost by 5 times. Conclusions. In the early period of development of experimental ovalbumin-induced allergic inflammation, the specific resistance of the respiratory system manifests itself in the form of activation of local links of cellular and humoral adaptive immunity, as evidenced by the dynamics of changes in the average number of lymphocytes (the maximum increasing coefficient 4.7 in the 1st experimental group), macrophages and plasma cells (maximum increasing coefficient 2.0 in the 1st experimental group) of diffuse lymphoid tissue of bronchi and lungs of guinea pigs.

scholarly journals Fish Bone Causing Perforation of the Intestine and Meckel’s Diverticulum

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Fakhar Shahid ◽  
Samir Omer Abdalla ◽  
Tamer Elbakary ◽  
Ahmad Elfaki ◽  
Syed Muhammad Ali
Keyword(s):  
Small Intestine ◽  
Abdominal Pain ◽  
Foreign Body ◽  
Bowel Perforation ◽  
Meckel’S Diverticulum ◽  
Fish Bone ◽  
Meckel's Diverticulum ◽  
Meckel Diverticulum ◽  
Common Site ◽  
Surgical Exploration

Perforation of small bowel due to ingested fish bone is rare, the most common site is ileum and occasionally, it can involve the appendix and/or Meckel diverticulum. We report six patients who, developed bowel perforation after fish bone ingestion, four of them found to have rent in the ileum and two through Meckel’s diverticulum and presented with abdominal pain and localized peritonitis. All underwent surgical exploration and removal of the fish bone and closure of the small intestine/excision of the diverticulum. Foreign body ingestion should be kept in mind in suspicious cases, and laparoscopy is very important to diagnose such rare cases as they may commonly be missed by imaging.

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