scholarly journals Oxidant/Antioxidant Balance in Cows and Sheep in Antenatal Pathology

2020 ◽  
Vol 10 (5) ◽  
pp. 26-28
Author(s):  
P. Sklyarov ◽  
S. Fedorenko ◽  
S. Naumenko
Keyword(s):  
Superoxide Dismutase ◽  
Blood Serum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Reduced Glutathione ◽  
Hemoglobin Content

It has been established that antenatal pathology causes changes in the oxidant/antioxidant balance indices of cows within the range from 24.3% to 41.5%, and in sheep - 17.2-26.2%. In particular, in cows the content of serum catalase decreased by 29.9% and that of superoxide dismutase - by 29.1%, in erythrocytes, catalase - by 35.7%, and reduced glutathione - by 28.0%, and by hemoglobin by 24.3%, the number of red blood cells - by 28.7% and the concentration of 2,3-diphosphoglycerate - by 41.5%. At the same time, the content of malondialdehyde in serum and erythrocytes, on the contrary, increased by 32.4% and 32.5%, respectively, and there was a change in oxidant/antioxidant balance from 1 : 1 to 1 : 3 conventional units. In sheep, the content of serum catalase decreased by 17.2% and that of superoxide dismutase - by 21.6%, in erythrocytes catalase - by 23.9% and in reduced glutathione - by 21.1%, hemoglobin content decreased by 26.2%, the number of erythrocytes - by 24,1% and the concentration of 2,3-diphosphoglycerate - by 21.2%. While the content of malondialdehyde in blood serum and in erythrocytes, on the contrary, increased by 23.7% and 22.3%, respectively, and the change in oxidant/antioxidant balance occurred from 2:1 to 1:3 conventional units.

scholarly journals Alterations in the Properties of Red Blood Cells in Men with Coronary Artery Diseases after Comprehensive Cardiac Rehabilitation

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Krzysztof Gwozdzinski ◽  
Anna Pieniazek ◽  
Joanna Bernasinska-Slomczewska ◽  
Joanna Brzeszczynska ◽  
Robert Irzmanski ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Membrane Protein ◽  
Cardiac Rehabilitation ◽  
Red Blood Cells ◽  
Membrane Fluidity ◽  
Exercise Test ◽  
Blood Cells ◽  
Reduced Glutathione ◽  
Osmotic Fragility ◽  
Cardiac Intervention

Purpose. Comprehensive cardiac rehabilitation (CCR) is a complex program aimed at improving the health status of patients with coronary artery disease (CAD), especially those who have been subjected to cardiac interventions (PCI and CABG).The aim of this study was to measure the changes in the properties of red blood cells (RBCs) in men with CAD after cardiac intervention and after participation in CCR program. Methods. In this study, we have investigated the influence of the physical training-based CCR program in 12 men with CAD, after PCI or CABG. The characteristics of RBCs including the basic morphology of RBCs, the conformational state of RBC membrane protein and hemoglobin, acetylcholinesterase activity, membrane fluidity, the osmotic fragility, and thiol concentration in membrane and in hemolysate were measured. Ascorbate concentration and reduced glutathione were also determined. The analysis was performed in men, before and after participation in CCR. The properties of RBCs were observed in connection with the exercise test, and parameters were evaluated before, immediately after, and 1 hour after the exercise test. Results. After CCR, a decrease in the mobility of erythrocyte membrane proteins was observed, which was accompanied by a decrease in lipid fluidity. In addition, immediately after the exercise test and 1 hour later, we measured a decrease in thiol level in hemolysate, but not in the plasma membrane. Unexpectedly, an increase in reduced glutathione concentration one hour after the exercise test after completing comprehensive cardiac rehabilitation was observed. Conclusion. CCR in men with CAD after cardiac intervention is connected with decreased membrane fluidity and decreased membrane protein mobility, which indicates that reduction of oxidative changes in these components occurs.

scholarly journals Fine Structure of the Red Pulp of the Spleen in Hereditary Spherocytosis

Blood ◽  
1972 ◽  
Vol 39 (1) ◽  
pp. 81-98 ◽  
Author(s):  
ZELMA MOLNAR ◽  
HENRY RAPPAPORT
Keyword(s):  
Endothelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hereditary Spherocytosis ◽  
Probable Mechanism ◽  
Hemoglobin Content ◽  
Sinus Endothelial Cells ◽  
In Transit ◽  
Conventional Light Microscopy ◽  
Red Pulp

Abstract The spleens from two children and one adult with hereditary spherocytosis were studied in the electron microscope. Stagnation of the erythrocytes within the splenic cords is attributable to their lack of plasticity as evidenced by the absence of bilobed, tailed, or squeezed forms in transit through the walls of the sinuses. In contrast to the sections studied by conventional light microscopy, the splenic sinuses in hereditary spherocytosis were not "empty," but contained red blood cells, the majority of which had lost their hemoglobin content. Cordal macrophages were increased in all three cases and were abundant in the splenic cords of the adult patient, causing a further impediment to the rapid passage of erythrocytes. Macrophages, and, to a lesser degree, sinus endothelial cells contained the products of hemoglobin breakdown. The macrophages showed active erythrophagocytosis. Sinus endothelial cells rarely contained intact red blood cells, but showed pronounced pinocytotic activity, a probable mechanism of hemoglobin incorporation. Platelets within the endothelial cells of the sinuses were much more frequently seen in the three cases of hereditary spherocytosis than in control spleens. The presence of ferritin in platelets suggests that they too may play a role in clearing the end products of hemolysis from the spleen.

Superoxide Dismutase (SOD) in Mouse Red Blood Cells Infected with Plasmodium berghei

1982 ◽  
Vol 68 (2) ◽  
pp. 337 ◽  
Author(s):  
Upsorn Suthipark ◽  
Jerapan Krungkrai ◽  
Amornrat Jearnpipatkul ◽  
Yongyuth Yuthavong ◽  
Bhinyo Panijpan
Keyword(s):  
Superoxide Dismutase ◽  
Red Blood Cells ◽  
Plasmodium Berghei ◽  
Blood Cells

Copper-zinc superoxide dismutase activity in red blood cells in probable Alzheimer's patients and their first-degree relatives

1994 ◽  
Vol 122 (2) ◽  
pp. 179-188 ◽  
Author(s):  
Jorge A. Serra ◽  
Arturo L. Famulari ◽  
Silvia Kohan ◽  
Enrique R. Marschoff ◽  
Raul O. Dominguez ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Superoxide Dismutase Activity ◽  
Copper Zinc Superoxide Dismutase ◽  
First Degree Relatives ◽  
Zinc Superoxide Dismutase ◽  
Copper Zinc

Influence of cadmium ions on the reduced glutathione and lipid peroxidation in the liver and red blood cells of mice

Biologija ◽  
2014 ◽  
Vol 59 (4) ◽  
Author(s):  
Kristina Čijauskaitė ◽  
Rasa Bernotienė ◽  
Leonid Ivanov
Keyword(s):  
Lipid Peroxidation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Reduced Glutathione ◽  
Cadmium Ions

Glutathione system in the blood of gastric cancer patients with various tumor histotypes and prevalence of the disease.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e16534-e16534
Author(s):  
Irina A. Goroshinskaya ◽  
Ekaterina I. Surikova ◽  
Elena M. Frantsiyants ◽  
Ludmila A. Nemashkalova ◽  
Polina S. Kachesova ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Glutathione Transferase ◽  
Reduced Glutathione ◽  
Reductase Activity ◽  
Cell Cancer ◽  
Low Grade ◽  
Glutathione System ◽  
Incidence And Mortality

e16534 Background: The role of oxidative stress in tumor growth and the multifaceted functional significance of glutathione have led to an interest in studying the components of the glutathione-dependent system in gastric cancer (GC), which occupies a leading position in cancer incidence and mortality. The aim of this work was to study the functioning of the glutathione system in the red blood cells of patients with gastric cancer in a comparative aspect depending on the histotype of the tumor and the prevalence of the disease. Methods: The study included 89 patients with GC divided into 6 groups depending on the histotype of the tumor. Separately, the results of the study were analyzed in patients with T4 status according to TNM and in patients with stage IV cancer. The content of reduced glutathione and the activity of glutathione-dependent enzymes were studied by conventional spectrophotometric methods in the red blood cells of patients. Statistical processing of the results was performed using the Statistika 6.0 program with Student’s t-test for two independent groups. Results: An increase in the content of glutathione in patients with GC compared with the group without oncopathology was revealed. The maximum increase was observed in patients with low-grade adenocarcinoma - by 42.5%, while in signet ring cell cancer (SRCC) there was only a tendency to increase by 17.8%. Glutathione reductase activity was reduced in adenocarcinoma by 23.4-26.2% and did not change in SRCC. The activity of the antioxidant enzymes glutathione peroxidase and glutathione transferase was increased in all groups and especially in SRCC - by 76% and 23-29%, respectively. In patients with T4 status and at stage 4 of the process, lower activity of the studied glutathione-dependent enzymes was revealed compared with all other groups of patients. Conclusions: The data indicate a greater functional potential of the glutathione system in SRCC. A significant increase in the activity of glutathione transferase with a sufficiently high level of reduced glutathione contributes to the development of treatment resistance in patients with SRCC.

Superoxide dismutase is preferentially degraded by a proteolytic system from red blood cells following oxidative modification by hydrogen peroxide

1988 ◽  
Vol 5 (5-6) ◽  
pp. 335-339 ◽  
Author(s):  
David C. Salo ◽  
Sharon W. Lin ◽  
Robert E. Pacifici ◽  
Kelvin J.A. Davies
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Oxidative Modification ◽  
Proteolytic System

scholarly journals Acoustic microscopy of red blood cells.

1988 ◽  
Vol 36 (10) ◽  
pp. 1341-1351 ◽  
Author(s):  
E A Schenk ◽  
R W Waag ◽  
A B Schenk ◽  
J P Aubuchon
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Signal Amplitude ◽  
Image Features ◽  
Acoustic Microscopy ◽  
Cell Deformation ◽  
Red Cell ◽  
Hemoglobin Content ◽  
Acoustic Microscope ◽  
Scanning Acoustic Microscope

We used a scanning acoustic microscope to image normal and outdated red blood cells, cells with different hemoglobin content, red cell ghosts, and cells treated with various drugs that induce echinocyte-stomatocyte transformation. Images were obtained at different planes of focus within the cells, corresponding to maxima and minima of signal intensity. Digitization and gray scale amplitude mapping were used to create axonometric plots that display signal amplitude variations within the cells. The images of red cells contain features produced by differences in topology, density, elasticity, and absorption. Both hemoglobin content and the cell cytoskeleton contribute to image features, and various deformations, characterized by the formation of blebs and vacuoles, are displayed in cells undergoing echinocyte-stomatocyte transformation. These preliminary findings, although mainly descriptive, indicate that acoustic microscopy may be a useful new method for evaluating red cell deformation and associated changes in mechanical properties.

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