scholarly journals Phylogenetic relationship of coffee leaf rust in the central jungle of Peru

2021 ◽  
Vol 70 (2) ◽  
Author(s):  
Delia Palmira Gamarra Gamarra ◽  
Gilberto Torres Suarez ◽  
Charo Milagros Villar Quiñonez ◽  
Alistair R. McTaggart ◽  
Emerson Clovis Carrasco Lozano
Keyword(s):  
Leaf Rust ◽  
Universal Primers ◽  
Phylogenetic Species Concept ◽  
Molecular Barcoding ◽  
San Luis ◽  
Rio Negro ◽  
Single Clade ◽  
Coffee Leaf Rust ◽  
Relationship Of ◽  
Production Losses

Coffee leaf rust is the main disease that causes significant losses in Coffea arabica. In Peru, this disease caused epidemics between 2008 and 2013 with production losses of 35 %. The objective was to identify H. vastatrix using a morphological and molecular approach based on a phylogenetic species concept. Coffee leaf samples with symptoms of chlorotic lesions with the presence of yellow uredospores at different severity stages of different cultivars were collected from 11 locations in the departments of Pasco and Junin during 2017-2018. DNA was purified as proposed by Cristancho and coworkers. The major subunit of ribosomal DNA was amplified with universal primers LR0R and LR5, and sequenced by Macrogen and deposited in GenBank. Sequences from the genera Achrotelium, Blastospora, Cystopsora, Hemileia, and Mikronegeria were included for phylogenetic analysis. The results showed that the rust was distributed in coffee growing regions of Pasco: Villa Rica (Catimor, Caturra, and Gran Colombia); Oxapampa (Yellow Caturra), and Junín: San Luis de Shuaro (Catimor), Chanchamayo (Catimor), San Ramón (Catimor), Vitoc (Caturra), Pichanaki (Caturra), Río Negro (Caturra), Pangoa (Yellow Caturra, Gran Colombia, Limani). It was also grouped into a single clade with isolated H. vastatrix from Mexico and Australia, suggesting that they come from a common ancestor. This is the first confirmed report using molecular barcoding of H. vastatrix in the central jungle of Peru.

scholarly journals Quantitative framework for coffee leaf rust (hemileia vastatrix), production and futures

2019 ◽  
Vol 7 (1) ◽  
pp. 77-87
Author(s):  
Laura H Kahn
Keyword(s):  
Supply Chain ◽  
Leaf Rust ◽  
Futures Prices ◽  
Coffee Plant ◽  
Hemileia Vastatrix ◽  
Coffee Rust ◽  
Coffee Leaf Rust ◽  
Production Losses

The livelihood of at least 120 million people worldwide depends on the coffee supply chain. Coffee rust is among the main diseases that attack the coffee plant and is caused by the Hemileia vastatrix fungus at temperatures between 10 - 30° C. Coffee rust infestation leads to production losses of over $1 billion annually worldwide. Coffee is the second largest traded commodity worldwide, with about $100 billion in volume traded annually. Understanding if there is a relationship between Temperature, Rainfall, Rust, Production and Futures coffee rust variables is important. This research offers the first known quantitative framework for describing and visualizing the correlation between coffee rust, amount of coffee produced and futures prices..

scholarly journals A fungus-eat-fungus world: Digitopodium, with particular reference to mycoparasites of the coffee leaf rust, Hemileia vastatrix

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Adans A. Colmán ◽  
Harry C. Evans ◽  
Sara S. Salcedo-Sarmiento ◽  
Uwe Braun ◽  
Kifle Belachew-Bekele ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Democratic Republic Of Congo ◽  
Sequence Data ◽  
Molecular Study ◽  
Classical Biological Control ◽  
Coffea Canephora ◽  
Molecular Data ◽  
Phylogenetic Position ◽  
Hemileia Vastatrix ◽  
Coffee Leaf Rust

AbstractDigitopodium hemileiae was described originally in 1930 as Cladosporium hemileiae; growing as a mycoparasite of the coffee leaf rust (CLR), Hemileia vastatrix, in a sample of diseased leaves of Coffea canephora collected in the Democratic Republic of Congo. No cultures from this material exist. More recently, the type material was re-examined and, based on morphological features, considered to be incorrectly placed in Cladosporium. The new genus Digitopodium was erected to accommodate this species. Interest in fungal antagonists of H. vastarix, as potential biocontrol agents of CLR, led to comprehensive surveys for mycoparasites, both in the African centre of origin of the rust, as well as in its South American exotic range. Among the rust specimens from Ethiopia, one was found to be colonized by a fungus congeneric with, and similar to, D. hemileiae. Pure cultures obtained from the Ethiopian material enabled a molecular study and for its phylogenetic position to be elucidated, based on DNA sequence data from the ITS and LSU regions. Molecular data showed that two members of the recently erected genus Hyalocladosporiella (Herpotrichiellaceae: Chaetothyriales) are congeneric with Digitopodium from Ethiopia and morphologically similar to both D. hemileiae and the two Ethiopian isolates. These isolates were found to be morphologically and genetically identical to H. tectonae, described previously from Brazil. Thus, species of Hyalocladosporiella are re-allocated to Digitopodium here; including D. tectonae, and a novel species, D. canescens, recently found in Brazil growing as a mycoparasite of Puccinia thaliae. The potential use of D. hemileiae and D. tectonae for classical biological control of CLR is discussed.

scholarly journals Effect of foliar application of potassium silicate on the progress of coffee leaf rust

2013 ◽  
Vol 38 (6) ◽  
pp. 547-551 ◽  
Author(s):  
Ueder Pedro Lopes ◽  
Laércio Zambolim ◽  
Pedro Nery Souza Neto ◽  
Antônio Fernando Souza ◽  
Alexandre Sandri Capucho ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Foliar Application ◽  
Potassium Silicate ◽  
Coffee Leaf Rust

scholarly journals Structural and functional analysis of genes with potential involvement in resistance to coffee leaf rust: a functional marker based approach

2019 ◽  
Author(s):  
Geleta Dugassa Barka ◽  
Eveline Teixeira Caixeta ◽  
Sávio Siqueira Ferreira ◽  
Laércio Zambolim
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Function Analysis ◽  
Bac Library ◽  
Leaf Rust Resistance ◽  
Molecular Data ◽  
Coffee Leaf Rust ◽  
Rust Resistance Genes

AbstractPhysiology-based differentiation of SH genes and Hemileia vastatrix races is the principal method employed for the characterization of coffee leaf rust resistance. Based on the gene-for-gene theory, nine major rust resistance genes (SH1-9) have been proposed. However, these genes have not been characterized at the molecular level. Consequently, the lack of molecular data regarding rust resistance genes or candidates is a major bottleneck in coffee breeding. To address this issue, we screened a BAC library with resistance gene analogs (RGAs), identified RGAs, characterized and explored for any SH related candidate genes. Herein, we report the identification and characterization of a gene (gene 11), which shares conserved sequences with other SH genes and displays a characteristic polymorphic allele conferring different resistance phenotypes. Furthermore, comparative analysis of the two RGAs belonging to CC-NBS-LRR revealed more intense diversifying selection in tomato and grape genomes than in coffee. For the first time, the present study has unveiled novel insights into the molecular nature of the SH genes, thereby opening new avenues for coffee rust resistance molecular breeding. The characterized candidate RGA is of particular importance for further biological function analysis in coffee.

scholarly journals Stability of Arabica coffee genotype (Coffea arabica L.) against leaf rust (Hemileia vastatrix)

2021 ◽  
Vol 748 (1) ◽  
pp. 012002
Author(s):  
Sabam Malau ◽  
Albiner Siagian ◽  
Maria Rumondang Sihotang
Keyword(s):  
Leaf Rust ◽  
Block Design ◽  
Research Result ◽  
Dry Season ◽  
Environment Interaction ◽  
Rust Disease ◽  
Hemileia Vastatrix ◽  
Arabica Coffee ◽  
Coffee Leaf Rust ◽  
Rust Severity

Abstract Coffee is now experiencing a serious threat from fungus Hemileia vastatrix which caused epidemic of rust disease in America, Africa, and Asia. As solution, the use of resistant cultivars is the best way. However, interaction between genotype and environment can change the rank of genotypes that shows instability of these genotypes against leaf rust. Purpose of this research was to study stability of genotypes of Arabica coffee against coffee leaf rust. A field experiment was arranged as factorial randomized complete block design with 2 factors (genotypes and climate zones) with three replication. The observed parameters were branch rust incidence, leaf rust incidence, and leaf rust severity. This research result showed significant genotype x environment interaction in all variables. Length of dry season is the most important factor affecting coffee leaf rust because it had the highest correlation coefficient with leaf rust severity (r = 0.662**). Less length of dry season should be the first criteria for selection of coffee farms. The most desired genotype was G7 which performed low leaf rust severity (7.71%) and had a stable resistance indicated by the same leaf rust severity in all environments and 6 SMg. Due to the significant interaction between genotypes and the environment, the genotype to be planted in a region must be tested in that region first.

scholarly journals Seasonal and altitudinal differences in coffee leaf rust epidemics on coffee berry disease-resistant varieties in Southwest Ethiopia

2019 ◽  
Vol 44 (3) ◽  
pp. 244-250 ◽  
Author(s):  
Gerba Daba ◽  
Kenny Helsen ◽  
Gezahegn Berecha ◽  
Bart Lievens ◽  
Adugna Debela ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Coffee Berry ◽  
Southwest Ethiopia ◽  
Coffee Berry Disease ◽  
Disease Resistant ◽  
Resistant Varieties ◽  
Coffee Leaf Rust
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