scholarly journals In vitro behavior of the dentin and enamel calcium hydroxyapatite in human premolars subjected to high temperatures

DYNA ◽  
2016 ◽  
Vol 83 (195) ◽  
pp. 34-41 ◽  
Author(s):  
Sebastián Medina ◽  
Liliana Salazar ◽  
Carlos Mejía ◽  
Freddy Moreno
Keyword(s):  
High Temperatures ◽  
Scientific Evidence ◽  
In Vitro Study ◽  
Calcium Hydroxyapatite ◽  
Forensic Sciences ◽  
Cross Sectional ◽  
Dental Identification ◽  
Dental Tissues ◽  
Reliable Marker

This paper details a cross-sectional, descriptive observational in vitro study of a pseudo-experimental nature that analyzes Electron Microscopy (SEM) by scanning the physical behavior of enamel and dentin calcium hydroxyapatite. The purpose is to describe the separation of these two mineralized dental tissues at the dentin-enamel junction (DEJ) when the teeth are subjected to high temperatures. This study provides scientific evidence that may broaden the discussion on the use of separation of the dentin-enamel junction as a constant and repetitive reliable marker for forensic use (use in forensic sciences) that can contribute to the dental identification process and documentation in a legal medical autopsy, given a situation in which bodies or human remains have been burned, carbonizated or incinerated.

scholarly journals Stereomicroscopic analysis of dental materials employed in endodontic exposed to high temperatures

2017 ◽  
Vol 19 (2) ◽  
Author(s):  
Johanna Aramburo ◽  
Angela Zapata ◽  
Sugey Zúñiga ◽  
Freddy Moreno
Keyword(s):  
High Temperatures ◽  
Dental Materials ◽  
In Vitro Study ◽  
Human Teeth ◽  
Great Resistance ◽  
Dental Tissues ◽  
Specific Rank ◽  
Hard Dental Tissues ◽  
Physical Changes

Objective: To describe the physical estereomicroscopical changes that occur in dental tissues and materials employed in a conventional endodontic treatment after application of high temperatures. Materials and methods: An in Vitro study was carried out to observe microscopic, structural and physical changes on hard dental tissues (enamel, dentine and cement) and on endodontic materials (gutta-percha Maillefer Dentsply®, endodontical cement based on oxide of zinc-eugenol Eufar®, cement based on epoxic resin Top Seal® Dentsply®, glass ionomer Fuji I® GC America®, silver amalgam GS80® SDI® and composite Point 4® Kerr®) in 124 human teeth, submitted to five temperature ranks (200ºC, 400ºC, 600ºC, 800ºC, 1000ºC). Results: The studied tissues and dental materials presented great resistance to the high temperatures without varying considerably their micro structure, such as that the physical changes (dimensional stability, cracks, pits, fractures, texture, color, carbonization and incineration) can be observed through estereomicrophotographies and can be associated to each specific rank of temperature. Conclusions: Some macrostructural changes of dental tissues and the dental materials occur of specific form in each rank of temperature, and for this reason they can be employed in the comparison before and postmortem during the process of identification of burned or charred corpse and human remains. Key words: Forensic dentistry, human identification, stereomicroscopy, dental materials, high temperatures.

scholarly journals Biomimetic Mechanism of Action of Fluoridated Toothpaste Containing Proprietary REFIX Technology on the Remineralization and Repair of Demineralized Dental Tissues: An in vitro Study

2020 ◽  
Author(s):  
Fabiano Vieira Vilhena ◽  
Sonia Mariza Luiz de Oliveira ◽  
Marcelo Henrick Maia Matochek ◽  
Pedro Luiz Santos Tomaz ◽  
Thales de Sá Oliveira ◽  
...  
Keyword(s):  
Mechanism Of Action ◽  
Elemental Analysis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Control Group ◽  
Cross Sectional ◽  
Dentinal Tubules ◽  
Dental Tissues ◽  
Ph Cycling

Abstract Objectives This in vitro study aimed to characterize the mineral content and surface and cross-sectional morphology of enamel and dentin tissues treated with a 1450 ppm fluoride-containing toothpaste with REFIX technology. Materials and Methods Bovine enamel blocks (n = 5) were obtained (4 × 4 × 6 mm), demineralized (artificial caries lesion), and treated (pH cycling and brushing with the toothpaste). During the pH cycling, which lasted for 7 days (demineralization and remineralization took 6 and 18 hours, respectively), the enamel was brushed for 5 minutes using an electric toothbrush before being immersed in a remineralizing solution. The dentin blocks were acid-etched for 2 minutes (0.05 M citric acid, pH 1.8) to expose the dentinal tubules (n = 5). Morphological analysis of the dentin was performed immediately and after 7 days of brushing with the dentifrice, and compared with the control group. The specimens were then cross-sectioned. The surface and cross-sectional micromorphology were assessed using scanning electron microscopy (SEM). The elemental analyses (weight%) were determined with an energy-dispersive X-ray spectroscopy (EDS). Results The toothpaste with REFIX technology remineralized and repaired the surface enamel effectively. The elemental analysis also demonstrated that treating the enamel with the toothpaste formed a silicon-enriched mineral layer on the enamel surface. Elemental analysis of the enamel cross-sections showed that the toothpaste induced a mineral change. The results were also consistent in the dentin, where the dentinal tubules were progressively occluded until there was complete occlusion after 7 days. Conclusions We prove the biomimetic mechanism of action of fluoridated toothpaste containing proprietary REFIX technology for obtaining silicon-enriched, remineralized and repaired dental tissues.

scholarly journals Effect of High Temperature on Composite as Post Endodontic Restoration in Forensic Analysis-An In Vitro Study

2014 ◽  
Vol 02 (02) ◽  
pp. 084-090
Author(s):  
Krishma Sharda ◽  
Varun Jindal ◽  
Ajay Chhabra ◽  
◽  
Keyword(s):  
High Temperatures ◽  
Digital Camera ◽  
In Vitro Study ◽  
Forensic Analysis ◽  
Vitro Study ◽  
Human Teeth ◽  
Great Resistance ◽  
Dental Tissues ◽  
Different Temperatures

Abstract Aim: The present study was done to evaluate and compare the effect of different temperatures (400 C, 800 C and 1000 C) on Composite as post endodontic restoration using Naked eye, Digital camera and Radiovisiography (RVG) in forensic analysis. Methodology: An in vitro study was conducted on 42 human teeth with composite as post endodontic restoration exposed to three temperature ranges: 400°C, 800°C and 1,000°C. Results: Composite material studied in the present research offered great resistance to high temperatures, without exhibiting considerable macrostructure variation, in such a way that physical changes (dimensional stability, fissures, cracks, fractures, texture, color, carbonization and incineration) can be identified and associated to each specific temperature range. Conclusion: Dental tissues and materials offer great resistance to the effect of high temperatures. Moreover, they present specific changes (color, texture, fissures, cracks fractures, fragmentation) which might contribute to the process of identifying a corpse, or burned, incinerated or carbonized human remains.

scholarly journals High Glucose-Mediated Cytokine Regulation in Gingival Fibroblasts and THP-1 Macrophage: a Possible Mechanism of Severe Periodontitis with Diabetes

2018 ◽  
Vol 50 (3) ◽  
pp. 973-986 ◽  
Author(s):  
Jung-Hwan Lew ◽  
Koji Naruishi ◽  
Yukari Kajiura ◽  
Yasufumi Nishikawa ◽  
Takahisa Ikuta ◽  
...  
Keyword(s):  
High Glucose ◽  
Gingival Crevicular Fluid ◽  
In Vitro Study ◽  
Inflammatory Factors ◽  
Diabetic Patients ◽  
Gingival Fibroblasts ◽  
Cross Sectional ◽  
Periodontal Tissues ◽  
Severe Periodontitis

Background/Aims: Diabetic patients are susceptible to severe periodontitis, but the precise mechanism is not fully understood. Aim of this study was to explore the biological pathogenesis of severe periodontitis in diabetic patients focusing on the crosstalk of human gingival fibroblasts (HGFs) and macrophages. Methods: A total of 70 periodontitis patients with or without diabetes mellitus (DM) were enrolled, and the statistical relationships of diabetic conditions to the periodontal inflammatory parameters were examined by cross-sectional study. In in vitro study, HGFs cell line CRL-2014® (ATCC) and differentiated THP-1 macrophages were cultured with normal glucose (NG: 5.5 mM) or high glucose (HG: 25 mM) condition, and treated with indicated inflammatory factors such as calprotectin (CPT), interleukin (IL)-1β and IL-6. To examine the effects of HG on soluble IL-6 receptor (sIL-6R) production in THP-1 macrophages, the supernatants were collected and the sIL-6R levels were measured by ELISA. To examine the effects of HG on IL-1β or IL-6-induced matrix metalloproteinase (MMPs) production in HGFs, the supernatants were collected. Levels of MMP-1 and tissue inhibitor of MMP-1 (TIMP-1) were measured by ELISA. Finally, after conditioned medium (CM) from THP-1 macrophages cultured with NG or HG conditions was collected, HGFs were treated with the CM. The supernatants were collected 24 hours later and the levels of MMP-1 and TIMP-1 were measured. To examine the specific effects of IL-1β contained in CM on MMP-1 and TIMP-1 production in HGFs, IL-1 receptor antagonist (IL-1ra) was used. Results: There were statistical correlation between IL-1β and sIL-6R levels in gingival crevicular fluid (GCF) and HbA1c in periodontitis patients with DM (IL-1β: P=0.035, sIL-6R: P=0.040). HG and CPT significantly induced sIL-6R production in THP-1 macrophages. HG significantly enhanced IL-1β or IL-6/sIL-6R-induced MMP-1 production in HGFs. The increase of MMP-1 by both IL-1β and IL-6/sIL-6R was significantly inhibited by specific ERK or IκB inhibitors. Corresponding to the regulation of MMP-1 production, HG condition increased the phosphorylation of p44/42 MAPK and IκBα in HGFs treated with IL-1β or IL-6/sIL-6R. Finally, MMP-1 production in HGFs cultured with HG increased significantly by CM from THP-1 macrophages cultured with HG. The induction of MMP-1 by the CM from THP-1 macrophages cultured with HG was significantly inhibited by dose dependent of IL-1ra in HGFs cultured with HG. Conclusion: Diabetic conditions such as HG induce IL-1β and sIL-6R production from macrophages in inflammatory periodontal tissues and may exacerbate the periodontitis synergistically via MMP-1 production from HGFs.

scholarly journals Macroscopic analysis of dental and periodontal tissues of pig (Sus domesticus) exposed to high temperatures

2017 ◽  
Vol 21 (1) ◽  
pp. 28-34
Author(s):  
Sebastián Medina ◽  
Sandra Henao ◽  
Viviana Muñóz ◽  
Carolina López ◽  
Juan Esteban Gutiérrez ◽  
...  
Keyword(s):  
High Temperatures ◽  
Alveolar Bone ◽  
Great Resistance ◽  
Dental Identification ◽  
Color Changes ◽  
Dental Tissues ◽  
Periodontal Tissues ◽  
Sus Domesticus ◽  
Physical Changes ◽  
Macroscopic Analysis

SUMMARYObjective: To describe the physical changesofmacro-structural dental tissues (enamel,dentin and cement) and periodontal(oral mucous membrane, alveolar compactbone and cancellous alveolar bone) ofdomestic pig (Sus domesticus) expose tohigh temperatures.Materials and methods: This descriptivestudy observed the physical changes in macro-structural dental tissues and periodontalin 25 teeth of domestic pigs subjected tohigh temperatures (200°C, 400°C, 600°C,800°C and 1000°C).Results: Dental and periodontal tissues studiedshow great resistance when subjectedto high temperatures without changing significantlytheir macro-structure. At 200°Cno color changes and cracks appear in theenamel. At 400°C there was an increase ofthe fissure and no separation between thehard tissues, initiating carbonization. At600°C fractures in the dental tissues andbone are most apparent. At 800°C burningof the tissues initiated. At 1000°C there wasno evidence of soft tissue.Conclusions: Macroscopic analysis of theteeth articulated in their alveolar-dentalunits constitutes a experimental model thatssimulates the changes of dental and periodontaltissues expose to high temperature.It is recommended to conduct astudy onhuman teeth in their respective unit articulatedalveolar to determine whether themacro-structural physical changes describedare repeated and can be extrapolated,and which can eventually be used duringthe process of dental identification anddocumentation of the medical legal autopsyused in the case of bodies or human remainsburned, charred and burned.Key words: Forensic dentistry, domesticpig (Sus domesticus), dental and periodontaltissues, temperature exposition, animalmodel.

scholarly journals In Vitro Study on the Effect of a New Bioactive Desensitizer on Dentin Tubule Sealing and Bonding

2020 ◽  
Vol 11 (2) ◽  
pp. 38
Author(s):  
Minh N. Luong ◽  
Laurie Huang ◽  
Daniel C. N. Chan ◽  
Alireza Sadr
Keyword(s):  
Bond Strength ◽  
In Vitro Study ◽  
Vitro Study ◽  
Acid Pretreatment ◽  
Universal Adhesive ◽  
Cross Sectional ◽  
Before And After ◽  
Human Dentin ◽  
Microshear Bond Strength

Bioactive mineral-based dentin desensitizers that can quickly and effectively seal dentinal tubules and promote dentin mineralization are desired. This in vitro study evaluated a novel nanohydroxyapatite-based desensitizer, Predicta (PBD, Parkell), and its effect on bond strength of dental adhesives. Human dentin discs (2-mm thick) were subjected to 0.5 M EDTA to remove the smear layer and expose tubules, treated with PBD, and processed for surface and cross-sectional SEM examination before and after immersion in simulated body fluid (SBF) for four weeks (ISO 23317-2014). The effects of two dental desensitizers on the microshear bond strength of a universal adhesive and a two-step self-etch system were compared. SEM showed coverage and penetration of nanoparticles in wide tubules on the PBD-treated dentin at the baseline. After four weeks in SBF, untreated dentin showed amorphous mineral deposits while PBD-treated dentin disclosed a highly mineralized structure integrated with dentin. Desensitizers significantly reduced microshear bond strength test (MSBS) of adhesives by 15–20% on average, depending on the bonding protocol. In conclusion, PBD demonstrated effective immediate tubules sealing capability and promoted mineral crystal growth over dentin and into the tubules during SBF-storage. For bonding to desensitizer-treated dentin, a two-step self-etching adhesive or universal bond with phosphoric acid pretreatment are recommended.

scholarly journals Conformation of Adjacent Self-expanding Stents: A Cross-Sectional In Vitro Study

2006 ◽  
Vol 29 (2) ◽  
pp. 255-259 ◽  
Author(s):  
Marion Hughes ◽  
Andrew R. Forauer ◽  
Mats Lindh ◽  
Wojciech Cwikiel
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Cross Sectional

scholarly journals Analysis of Enamel Loss by Prophylaxis and Etching Treatment in Human Tooth Using Optical Coherence Tomography: An In Vitro Study

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Naresh Kumar Ravichandran ◽  
Hemanth Tumkur Lakshmikantha ◽  
Hyo-sang Park ◽  
Mansik Jeon ◽  
Jeehyun Kim
Keyword(s):  
Optical Coherence Tomography ◽  
In Vitro Study ◽  
Enamel Surface ◽  
Human Tooth ◽  
Tooth Surface ◽  
Optical Coherence ◽  
Cross Sectional ◽  
Noninvasive Imaging Technique ◽  
Treatment Procedures

Bonding of braces is an essential part in contemporary orthodontic treatment. For the proper strength of bracket bonding, enamel conditioning or surface treatment on tooth surface is required. Treatment on the tooth surface such as prophylaxis smoothing with pumice and enamel etching results in considerable damages to the enamel surface of the tooth. In this study, we have proposed optical coherence tomography as a noninvasive imaging technique for the evaluation of damage induced during such treatment procedures. Using depth intensity analysis of the obtained cross-sectional images, the damage resulting to the enamel surface was studied after prophylaxis smoothening and etching steps.

Effect of high temperatures on teeth fixed with an orthodontic bracket. An in vitro study

2020 ◽  
Vol 308 ◽  
pp. 110182
Author(s):  
Gretel González-Colmenares ◽  
Liliana Calvo-Díaz ◽  
Marcela Nastul-Enríquez ◽  
María Mónica Bertel-Ruíz ◽  
Ingrid Garzón-Ramírez ◽  
...  
Keyword(s):  
High Temperatures ◽  
In Vitro Study ◽  
Vitro Study ◽  
Orthodontic Bracket
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