Single nucleotide polymorphism in STAT5A could not endorse variation in milk production traits in Indian bovine population

2021 ◽  
Author(s):  
Sudhakar Krovvidi ◽  
Thiruvenkadan K. Aranganoor ◽  
Saravanan Ramasamy ◽  
Murali Nagarajan
Keyword(s):  
Milk Production ◽  
Milk Fat ◽  
Bovine Milk ◽  
Fat Content ◽  
Production Traits ◽  
Crossbred Cattle ◽  
Milk Production Traits ◽  
Milk Traits ◽  
Murrah Buffaloes ◽  
Genetic Groups

Abstract The Signal Transducer and Activator of Transcription 5A (STAT5A) gene involved in activating the transcription of milk protein genes was predicted to be influencing milk production traits. The present study was undertaken to investigate the suitability of the polymorphism of STAT5A as a marker for milk traits in Ongole, crossbred cattle and Murrah buffaloes from Southern India. Blood samples (n = 502) for DNA isolation and milk samples (n = 222) from different genetic groups were collected from various farms. The gene variants upon polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) on the exon 7 region of STAT5A were subjected to GLM analysis to evaluate their association with milk production traits. The frequencies of C and T alleles at the STAT5A/AvaI locus were 0.98 and 0.02 (Jersey crossbred), 0.94 and 0.06 [Holstein-Friesian (HF) crossbred], 0.97 and 0.03 (Ongole). T allele was not observed in Murrah buffaloes. The least squares mean lactation milk yield of CC and CT genotypes of STAT5A were 2,096.90 ± 48.63 and 2,294.41 ± 215.85 kg in Jersey crossbred, 2,312.92 ± 91.01 and 2,392.82 ± 207.66 kg in HF crossbred and 528.40 ± 22.10 and 396.37 ± 76.17 kg in Ongole cattle, respectively. The milk fat content of the CC genotype was higher (P > 0.05) in Jersey crossbred cattle. The CT genotypes of Ongole and HF crossbred cattle recorded a higher fat per cent than the CC genotypes. Significant associations were not observed in support of STAT5A as a marker for milk production traits in either Ongole or crossbred cattle of indicine admixture and no reason could be found to consider this locus as universal markers for milk production traits in indicine cattle and buffaloes. Considering the monomorphic nature of the gene in buffaloes and their higher milk fat content as compared to bovine milk, much remains to be explored regarding the underlying differences across the bovine and the bubaline species.

An association analysis between PRL genotype and milk production traits in Italian Mediterranean river buffalo

2017 ◽  
Vol 84 (4) ◽  
pp. 430-433 ◽  
Author(s):  
Jun Li ◽  
Aixin Liang ◽  
Zipeng Li ◽  
Chao Du ◽  
Guohua Hua ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Fat Content ◽  
Phenotypic Variance ◽  
River Buffalo ◽  
Production Traits ◽  
Milk Production Traits ◽  
Exon 2 ◽  
Mediterranean River

This Research Communication describes the association between genetic variation within the prolactin (PRL) gene and the milk production traits of Italian Mediterranean river buffalo (Bufala mediterranea Italiana). High resolution melting (HRM) techniques were developed for genotyping 465 buffaloes. The association of genetic polymorphism with milk production traits was performed and subsequently the effects of parity and calving season were evaluated. Single nucleotide polymorphisms (SNPs) were identified at exons 2 and 5 and at introns 1 and 2. All the SNPs were in Hardy–Weinberg equilibrium, and statistical analysis showed that the polymorphism of intron1 was significantly (P < 0·05) associated with milk yield, milk protein content and peak milk yield. The average contribution of the intron1 genotype (r2intron1) to total phenotypic variance in milk production traits was 0·09, and the TT genotype showed lower values than CC and CT genotypes. A nonsynonymous SNP was identified in exon 2, which resulted in an amino acid change from arginine to cysteine. Moreover, the polymorphism of exon 2 was associated significantly with milk fat content (P < 0·05), and the buffaloes with TT genotype showed higher total fat content than the buffaloes with CT genotype. These findings provide evidence that polymorphisms of the buffalo PRL gene are associated with milk production traits and PRL can be used as a candidate gene for marker-assisted selection in Italian Mediterranean river buffalo breeding.

Season and reproductive status rather than genetic factors influence change in ewe weight and fat over time. 4. Genetic relationships of ewe weight and fat score with fleece, reproduction and milk traits

2016 ◽  
Vol 56 (4) ◽  
pp. 708 ◽  
Author(s):  
S. F. Walkom ◽  
F. D. Brien ◽  
M. L. Hebart ◽  
N. M. Fogarty ◽  
S. Hatcher ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Fat ◽  
Genetic Relationships ◽  
Fibre Diameter ◽  
Reproductive Traits ◽  
Production Cycle ◽  
Production Traits ◽  
Milk Production Traits ◽  
Milk Traits ◽  
Fat Score

The profitability of a sheep enterprise is greatly influenced by the ability of the ewe to produce a lamb annually. This paper examines the between- and within-breed association between the liveweight and fat score of the adult ewe and key fleece, reproduction and milk production traits. The study analysed the performance of 2846 first-cross Merino and Corriedale ewes from the maternal central progeny test (MCPT) over their first three production cycles. Breed (9 sire breeds) and within-breed (91 sires) correlations were calculated from bivariate correlations of adult liveweight and fat score with fleece, reproduction and milk production traits. The mean liveweight and fatness of the breeds were low to moderately negatively correlated with greasy fleece weight (–0.55 and –0.25, respectively) and moderate to strongly positively correlated with fibre diameter (0.88 and 0.41, respectively). However, the within-breed correlations were generally low to negligible. The genetic relationships between ewe fat score and reproductive traits at the breed and within-breed level was generally low to negligible and, in some cases, negative under production conditions where the ewes were well managed and averaged a fat score of 3.5 pre-joining and became too fat across the production cycle. Strong breed correlations between ewe fat score and milk fat concentration (0.88) suggest that selection of fatter sire breeds will lead to increased fat in their progenies’ milk, which was associated with larger lambs. The potential advantages of selecting for more fat to improve the maternal performance of the ewe seem to be limited in enterprises where the production system can easily be adjusted to restrict the influence of temporary and long-term feed deficiencies, as was achieved within the MCPT research flocks.

Investigation of myostatin and calpain 3 gene polymorphisms and their association with milk-production traits in Sfakia sheep

2020 ◽  
Vol 60 (3) ◽  
pp. 347
Author(s):  
Adel H. M. Ibrahim ◽  
Nikolaos Tzanidakis ◽  
Smaragda Sotiraki ◽  
Huitong Zhou ◽  
Jonathan Hickford
Keyword(s):  
Nucleotide Sequence ◽  
Milk Production ◽  
Milk Yield ◽  
Milk Fat ◽  
Fat Content ◽  
Solid Content ◽  
Production Traits ◽  
Milk Production Traits ◽  
Banding Patterns ◽  
Milk Solid

Context Genetic selection based on genetic markers for economically important traits in Sfakia sheep. Aims The aim of the present study was to investigate variation in the ovine myostatin gene (MSTN) and calpain 3 gene (CAPN3), and their association with milk-production traits. Methods Records for milk yield, milk fat content, protein content, lactose content, and non-fat solid content, pH and somatic-cell score (log), were obtained from 376 Sfakia ewes. Polymerase chain reaction–single-strand conformational polymorphism (PCR–SSCP) analyses were used to detect variation in intron 1 of MSTN and exon 10 of CAPN3. General linear models were then used to test for associations between the variation in MSTN and CAPN3, and milk-production traits. Key results The SSCP banding patterns for MSTN showed four variants (A1, A2, A3 and A4), which contained nine nucleotide sequence differences. Four SSCP banding patterns (C1, C2, C3 and C4) were observed for CAPN3 and these contained eight nucleotide-sequence differences. The MSTN variation was associated (P &lt; 0.05) with variation in milk yield and non-fat milk solid content. Variation in CAPN3 was associated with milk yield (P &lt; 0.001), fat content (P &lt; 0.05) and lactose content (P &lt; 0.05). Association analyses between the presence/absence of MSTN and CAPN3 variants and milk-production traits showed that a variant of MSTN that had previously between associated with muscle hypertrophy was associated with decreased milk yield (P &lt; 0.05) and a lower non-fat milk solid content (P &lt; 0.01). A CAPN3 variant that had previously been associated with increased sheep-carcass loin lean-meat yield was associated with a decreased milk yield (P &lt; 0.01) and a decreased milk fat content (P &lt; 0.05). Conclusions Our results have provided an insight into the effects of variation in ovine MSTN and CAPN3 on milk-production traits in sheep. Implications To preserve the dual-purpose characteristics of Sfakia sheep, breeding goals should take into account the possible antagonism between meat and milk traits.

scholarly journals A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Pauline Martin ◽  
Isabelle Palhière ◽  
Cyrielle Maroteau ◽  
Philippe Bardou ◽  
Kamila Canale-Tabet ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Fat ◽  
Genome Scan ◽  
Fat Content ◽  
Production Traits ◽  
Milk Production Traits ◽  
Dairy Goats ◽  
A Genome ◽  
New Mutations

scholarly journals ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traitsin Jersey cows

2010 ◽  
Vol 55 (No. 11) ◽  
pp. 463-467 ◽  
Author(s):  
H. Kulig ◽  
I. Kowalewska-Łuczak ◽  
M. Kmieć ◽  
K. Wojdak-Maksymiec C
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Milk Production ◽  
Milk Fat ◽  
Fatty Acid Transport ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Acid Transport ◽  
Milk Production Traits ◽  
Milk Traits

Milk components originating from blood plasma substrates are synthesized in epithelial cells of the mammary gland. Milk lipids are synthesized from fatty acids which bind to specific proteins &ndash; FABPs (fatty acid binding proteins). FABPs are a family of small cytoplasmic proteins; nine members of the family have been identified so far (FABP1&ndash;FABP9) (Chmurzyńska et al., 2006). Their main roles include fatty acid uptake, transport and metabolism. FABPs can modulate the fatty acid concentration in cells and therefore they affect different cellular processes, especially lipid metabolism. FABP3 and FABP4 are present in tissues with a high demand for fatty acids, such as heart muscle, skeletal muscles, lactating mammary gland, liver or adipose tissue (Roy et al., 2003). FABP3 gene was mapped to bovine chromosome 2 (Calvo et al., 2004), where QTLs affecting milk fat yield and content were described (Khatkar et al., 2004). FABP4 gene was mapped to BTA14 (Michal et al., 2006), which is very rich in QTLs for milk production traits (Khatkar et al., 2004). Fatty acid transport is assisted by the specific proteins called FATPs (fatty acid transport proteins). This protein group includes SLC27A3 (solute carrier family 27, member 3). It belongs to the family of proteins that facilitate long-chain fatty acid transport across the cytoplasmic membrane. Another protein with similar functions is ANXA9 (annexin A9), the member of Ca2+ and phospholipid-binding protein family (Calvo et al., 2006b). Genes encoding SLC27A3 and ANXA9 were mapped to chromosome 3, within the region where QTLs for milk fat content and the other milk traits have been mapped. Both of the above-mentioned genes are expressed in the mammary gland (Calvo et al., 2006b). The polymorphic sites (SNPs &ndash; single nucleotide polymorphisms) within the bovine FABP3, FABP4, SLC27A3 and ANXA9 genes were identified (Wu et al., 2005; Calvo et al., 2006b; Michal et al., 2006; Cho et al., 2008). Associations between SNPs in these genes and milk production traits in cattle have not been reported so far. However, polymorphism in the FABP4 gene has been significantly associated with carcass traits in cattle (Michal et al., 2006; Cho et al., 2008). Due to their expression sites, physiological properties and chromosomal localisation, the described genes might be considered as candidate genes for milk production traits. The aim of this study was to determine allele and genotype frequencies and to establish possible associations between the ANXA9, SLC27A3, FABP3 and FABP4 SNPs, and selected milk traits in Jersey cows.

scholarly journals ANXA9, SLC27A3, FABP3 and FABP4 single nucleotide polymorphisms in relation to milk production traits in Jersey cows

2010 ◽  
Vol 55 (No. 1) ◽  
pp. 463-467 ◽  
Author(s):  
H. Kulig ◽  
I. Kowalewska-Łuczak ◽  
M. Kmieć ◽  
K. Wojdak-Maksymiec
Keyword(s):  
Mammary Gland ◽  
Fatty Acid ◽  
Milk Production ◽  
Milk Fat ◽  
Fatty Acid Transport ◽  
Nucleotide Polymorphisms ◽  
Production Traits ◽  
Acid Transport ◽  
Milk Production Traits ◽  
Milk Traits

Milk components originating from blood plasma substrates are synthesized in epithelial cells of the mammary gland. Milk lipids are synthesized from fatty acids which bind to specific proteins &ndash; FABPs (fatty acid binding proteins). FABPs are a family of small cytoplasmic proteins; nine members of the family have been identified so far (FABP1&ndash;FABP9) (Chmurzyńska et al., 2006). Their main roles include fatty acid uptake, transport and metabolism. FABPs can modulate the fatty acid concentration in cells and therefore they affect different cellular processes, especially lipid metabolism. FABP3 and FABP4 are present in tissues with a high demand for fatty acids, such as heart muscle, skeletal muscles, lactating mammary gland, liver or adipose tissue (Roy et al., 2003). FABP3 gene was mapped to bovine chromosome 2 (Calvo et al., 2004), where QTLs affecting milk fat yield and content were described (Khatkar et al., 2004). FABP4 gene was mapped to BTA14 (Michal et al., 2006), which is very rich in QTLs for milk production traits (Khatkar et al., 2004). Fatty acid transport is assisted by the specific proteins called FATPs (fatty acid transport proteins). This protein group includes SLC27A3 (solute carrier family 27, member 3). It belongs to the family of proteins that facilitate long-chain fatty acid transport across the cytoplasmic membrane. Another protein with similar functions is ANXA9 (annexin A9), the member of Ca2+ and phospholipid-binding protein family (Calvo et al., 2006b). Genes encoding SLC27A3 and ANXA9 were mapped to chromosome 3, within the region where QTLs for milk fat content and the other milk traits have been mapped. Both of the above-mentioned genes are expressed in the mammary gland (Calvo et al., 2006b). The polymorphic sites (SNPs &ndash; single nucleotide polymorphisms) within the bovine FABP3, FABP4, SLC27A3 and ANXA9 genes were identified (Wu et al., 2005; Calvo et al., 2006b; Michal et al., 2006; Cho et al., 2008). Associations between SNPs in these genes and milk production traits in cattle have not been reported so far. However, polymorphism in the FABP4 gene has been significantly associated with carcass traits in cattle (Michal et al., 2006; Cho et al., 2008). Due to their expression sites, physiological properties and chromosomal localisation, the described genes might be considered as candidate genes for milk production traits. The aim of this study was to determine allele and genotype frequencies and to establish possible associations between the ANXA9, SLC27A3, FABP3 and FABP4 SNPs, and selected milk traits in Jersey cows.

scholarly journals The Association of Four Polymorphisms within the Insulin-Like Growth Factor 1 Receptor Gene with Milk Production Traits in Simmental Cows

2016 ◽  
Vol 16 (4) ◽  
pp. 1029-1044 ◽  
Author(s):  
Małgorzata Szewczuk
Keyword(s):  
Growth Factor ◽  
Milk Production ◽  
Milk Fat ◽  
Receptor Gene ◽  
Insulin Like Growth Factor ◽  
Production Traits ◽  
Milk Production Traits ◽  
Gene Encoding ◽  
Milk Traits ◽  
Factor Type

Abstract The aim of this study was to determine the frequency of alleles and genotypes of four polymorphisms located in exons 2, 10 and 16 of the gene encoding insulin-like growth factor type 1 receptor (IGF1R) in the tested herd of 242 Simmental cows and to search for the relationship between these polymorphisms and selected milk production traits. The study applied the following methods: PCR-RFLP and combination of nested PCR and ACRS-PCR. The presence of three genotypes was found for all SNPs. The frequency of alleles was as follows: C - 0.29 and T - 0.71 (IGF1R/e2/MspI), A - 0.33 and G - 0.67 (IGF1R/e2/TaqI), C - 0.77 and T - 0.23 (IGF1R/e10/MspI) and C - 0.53 and T - 0.47 (IGF1R/e16/RsaI). In all lactations, cows with TT (IGF1R/e2/MspI) and GG genotypes (IGF1R/e2/TaqI) produced the highest amounts of milk, fat and protein (P≤0.01), particularly individuals with the combined TT/GG genotypes. As regards the IGF1R/e10/MspI and IGF1R/e16/ RsaI genotypes, the highest milk, fat and protein yields were observed in cows with separate and combined CC/CC genotypes, while the lowest in animals with combined TT/TT genotypes. Cumulative analysis of all genotype combinations showed that individuals with a potentially best combination of TT/GG/CC/CC might be characterized by the highest milk yield as well as fat and protein content in milk. Potentially unfavorable combinations (such as CC/AA/TT/TT and similar) have been almost completely eliminated from the herd tested. The IGF1R gene is proposed as a candidate gene for milk traits in cattle.

scholarly journals Author Correction: A genome scan for milk production traits in dairy goats reveals two new mutations in Dgat1 reducing milk fat content

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Pauline Martin ◽  
Isabelle Palhière ◽  
Cyrielle Maroteau ◽  
Philippe Bardou ◽  
Kamila Canale-Tabet ◽  
...  
Keyword(s):  
Milk Production ◽  
Milk Fat ◽  
Genome Scan ◽  
Fat Content ◽  
Production Traits ◽  
Milk Production Traits ◽  
Dairy Goats ◽  
A Genome ◽  
New Mutations

Factors influencing milk osteopontin concentration based on measurements from Danish Holstein cows

2021 ◽  
pp. 1-6
Author(s):  
Brian Christensen ◽  
Elias D. Zachariae ◽  
Nina A. Poulsen ◽  
Albert J. Buitenhuis ◽  
Lotte B. Larsen ◽  
...  
Keyword(s):  
Milk Production ◽  
Bovine Milk ◽  
Farm Management ◽  
Holstein Cows ◽  
Enzyme Linked Immunosorbent Assay ◽  
Production Traits ◽  
Milk Production Traits ◽  
Factors Influencing ◽  
Significant Interest ◽  
Variation Explained

Abstract Our objective was to determine the content of the bioactive protein osteopontin (OPN) in bovine milk and identify factors influencing its concentration. OPN is expressed in many tissues and body fluids, with by far the highest concentrations in milk. OPN plays a role in immunological and developmental processes and it has been associated with several milk production traits and lactation persistency in cows. In the present study, we report the development of an enzyme linked immunosorbent assay (ELISA) for measurement of OPN in bovine milk. The method was used to determine the concentration of OPN in milk from 661 individual Danish Holstein cows. The median OPN level was determined to 21.9 mg/l with a pronounced level of individual variation ranging from 0.4 mg/l to 67.8 mg/l. Breeding for increased OPN in cow's milk is of significant interest, however, the heritability of OPN in milk was found to be relatively low, with an estimated value of 0.19 in the current dataset. The variation explained by the herd was also found to be low suggesting that OPN levels are not affected by farm management or feeding. Interestingly, the concentration of OPN was found to increase with days in milk and to decrease with parity.

Association of the Leu127 variant of the bovine growth hormone (bGH) gene with increased yield of milk, fat, and protein in Australian Holstein-Friesians

2000 ◽  
Vol 51 (4) ◽  
pp. 515 ◽  
Author(s):  
M. R. Shariflou ◽  
C. Moran ◽  
F. W. Nicholas
Keyword(s):  
Growth Hormone ◽  
Milk Production ◽  
Milk Fat ◽  
Bovine Growth Hormone ◽  
Data Sets ◽  
Production Traits ◽  
Milk Production Traits ◽  
Maximum Benefit ◽  
Pcr Products ◽  
Trait Locus

The occurrence of the Leu127/Val127 variants of the bovine growth hormone (bGH) gene and their effect on milk production traits was investigated in Australian Holstein-Friesian cattle. Animals were genotyped for the Leu127/Val127 variants, with RFLP methodology, using PCR and AluI digestion of PCR products (AluI-RFLP). Alleles Leu127 and Val127 occurred with frequencies of 82% and 18%, respectively. The quantitative effect of this polymorphic site on milk-production traits was estimated from lactation data and test-day data. Results from the 2 data sets consistently showed that the Leu127 allele is associated with higher production of milk, fat, and protein and is dominant to Val127. The average effects of the gene substitution are 95 L for milk yield, 7 kg for fat yield, and 3 kg for protein yield per lactation. This locus may be directly responsible for quantitative variation or it may be a marker for a closely linked quantitative trait locus (QTL) for milk-production traits in Australian dairy cattle. In either case, it will be useful as an aid to selection for improvement of milk production traits. As the Leu127 allele is dominant, selection of AI sires homozygous for the Leu127 allele (Leu127/Leu127) will result in maximum benefit without the need for genotyping cows.

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