scholarly journals A NEW METHOD FOR THE QUANTITATIVE DETERMINATION OF PROTEIN OF BACTERIAL ORIGIN ON THE BASIS OF D-ASPARTIC ACID AND D-GLUTAMIC ACID CONTENT

2001 ◽  
Vol 30 (1) ◽  
pp. 37-52 ◽  
Author(s):  
J. Csapó ◽  
J. Schmidt ◽  
Zs. Csapó-Kiss ◽  
G. Holló ◽  
I. Holló ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Acid Content ◽  
New Method ◽  
Bacterial Origin

Quantitative determination of protein of bacterial origin on the basis ofd-aspartic acid andd-glutamic acid content

2002 ◽  
Vol 56 (1) ◽  
pp. S169-S171 ◽  
Author(s):  
J. Csapó ◽  
J. Schmidt ◽  
Zs. Csapó-Kiss ◽  
É. Varga-Visi ◽  
G. Pohn ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Acid Content ◽  
Bacterial Origin

Quantitative Determination of Glycine, Alanine, Aspartic Acid, Glutamic Acid, Phenylalanine, and Tryptophan by Raman Spectroscopy

2017 ◽  
Vol 50 (4) ◽  
pp. 651-662 ◽  
Author(s):  
Yasushi Numata ◽  
Maria Otsuka ◽  
Kenji Yamagishi ◽  
Hiroyuki Tanaka
Keyword(s):  
Raman Spectroscopy ◽  
Quantitative Determination ◽  
Glutamic Acid ◽  
Aspartic Acid

AGGLUTINATION INHIBITION REACTIONS FOR THE DETERMINATION OF GONADOTROPHINS

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S95-S112 ◽  
Author(s):  
A. H. W. M. Schuurs
Keyword(s):  
Immune System ◽  
Quantitative Determination ◽  
Haemagglutination Inhibition ◽  
Latex Agglutination ◽  
Literature Survey ◽  
New Method ◽  
Test Fluid ◽  
Main Application ◽  
Test Systems

ABSTRACT Various techniques for sensitising erythrocytes and latex particles with gonadotrophins, particularly with HCG, are described. The haemagglutination inhibition reactions are generally interpreted by means of »erythrocyte settling patterns«. By a new method of evaluating these patterns a relatively precise quantitative determination is possible. Latex agglutination inhibition reactions on slides are particularly suitable as rapid qualitative tests. In cases where the maximum attainable sensitivity of the agglutination inhibition tests is insufficient, e. g. for determining LH concentrations in urine, the hormone in the test fluid has to be concentrated or extracted. An alternative method is a modified haemagglutination inhibition test for large volumes which is applicable to unconcentrated urine. Due to non-specific inhibitions the above-mentioned tests cannot be applied to unprocessed serum. Agglutination inhibition tests with HCG are already well advanced, pregnancy diagnosis being their main application. Now that highly purified HCG is available, a satisfactory specificity for these tests can be attained. If the immune system for HCG is used for estimating LH, it has to meet additional specificity requirements. Furthermore, the measure of cross-reaction and the choice of standard merit special attention. Finally, a literature survey is given of test systems in which LH and FSH were used as antigens.

Quantitative determination of p-iodobenzoyl-L-glutamic acid and barium salt of p-methylaminobenzoyl-L-glutamic acid

1981 ◽  
Vol 15 (5) ◽  
pp. 364-366
Author(s):  
N. A. Shikhaleeva ◽  
M. S. Belyakova ◽  
B. D. Statkevich
Keyword(s):  
Quantitative Determination ◽  
Glutamic Acid ◽  
Barium Salt

scholarly journals ON THE CALCULATION OF "TURNOVER TIME" AND "TURNOVER RATE" FROM EXPERIMENTS INVOLVING THE USE OF LABELING AGENTS

1943 ◽  
Vol 26 (3) ◽  
pp. 325-331 ◽  
Author(s):  
D. B. Zilversmit ◽  
C. Entenman ◽  
M. C. Fishler
Keyword(s):  
Quantitative Determination ◽  
Turnover Rate ◽  
Turnover Time ◽  
New Method ◽  
Animal Body

1. A new method for the determination of an immediate precursor of a substance occurring in the animal body is presented. 2. Calculations on the quantitative determination of the rate of turnover of a substance and their application to experiments involving the use of labeling agents are given. These calculations take into account loss of the isotopic substance by way of breakdown or transport.

The Oocytes of the Goby Pomatoschistus minutus. III. Determination of Amino Acid Component

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1094-1095
Author(s):  
Rüdiger Riehl
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Acid Content ◽  
Amino Acid Content ◽  
Acid Component ◽  
Pomatoschistus Minutus ◽  
Protein Amino Acids ◽  
Amino Acid Component

Abstract The oocytes of the marine goby Pomatoschistus minutus were analyzed for their amino acid content. Most of the amino acids exist as protein, only a little part is free or peptide-bound. Among the protein-bound amino acids, high levels of glutamic acid, proline, alanine, aspartic acid, valine and leucine were detected. These represent more than 60% of the protein amino acids. Among the free acids, glutamic acid, serine and alanine, are dominant. There are no certain proofs of the occurrence of peptide pools in the oocytes of Pomatoschistus minutus.

A new method for separation and quantitative determination of metabolites of tryptamines in biological material

1969 ◽  
Vol 25 (3) ◽  
pp. 435-440 ◽  
Author(s):  
Sonja Iskrić ◽  
Lucija Stančić ◽  
S. Kveder
Keyword(s):  
Quantitative Determination ◽  
Biological Material ◽  
New Method

scholarly journals A new method for quantitative determination of protein associated with crustacean chitin.

1987 ◽  
Vol 61 (4) ◽  
pp. 437-441 ◽  
Author(s):  
Yasuyuki TAKIGUCHI ◽  
Hiroshi YAMASHITA ◽  
Kazuhiro OHKOUCHI ◽  
Kenzo SHIMAHARA
Keyword(s):  
Quantitative Determination ◽  
New Method

scholarly journals Pharmacokinetics and Bioavailability Study of Monocrotaline in Mouse Blood by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Lianguo Chen ◽  
Bin Zhang ◽  
Jinlai Liu ◽  
Zhehua Fan ◽  
Ziwei Weng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Quantitative Determination ◽  
Ultra Performance Liquid Chromatography ◽  
New Method ◽  
Tandem Mass ◽  
Mouse Blood ◽  
Detection Range

Background and Aims. The present study aimed to develop a simple and sensitive method for quantitative determination of monocrotaline (MCT) in mouse blood employing ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI/MS/MS) using rhynchophylline as an internal standard. Methods. Proteins present in the blood samples were precipitated using acetonitrile. MCT was separated using a 1.7-μm ethylene bridged hybrid (BEH) C18 column (2.1 mm × 50 mm) with a gradient elution program and a constant flow rate of 0.4 mL/min. The LC mobile phase consisted of 10 mmol/L ammonium acetate (containing 0.1% formic acid) and acetonitrile. The total elution time was 4.0 min. The analytes were detected on a UPLC-ESI mass spectrometer in multiple reaction monitoring (MRM) mode and quantified. Results. The new method for the determination of MCT has a satisfactory linear detection range of 1-2000 ng/mL and excellent linearity (r = 0.9971). The lower limit of quantification (LLOQ) of MCT is 1.0 ng/mL. Intra- and interassay precisions of MCT were ≤13% with an accuracy from 96.2% to 106.6%. The average recovery of the new method was >75.0%, and matrix effects were between 89.0% and 94.3%. Based on the pharmacokinetics data, the bioavailability of MCT in mice was 88.3% after oral administration. Conclusions. The results suggest that the newly standardized method for quantitative determination of MCT in whole blood is fast, reliable, specific, sensitive, and suitable for pharmacokinetic studies of MCT after intravenous or intragastric administration.

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