Chemical composition andin vitroantioxidant activity of hydro-ethanolic extracts fromBauhinia forficatasubsp.pruinosaandB. variegata

2013 ◽  
Vol 64 (1) ◽  
pp. 21-33 ◽  
Author(s):  
Carla Sayago ◽  
Vanessa Camargo ◽  
F. Barbosa ◽  
Cláudia Gularte ◽  
Geovana Pereira ◽  
...  
2015 ◽  
Vol 43 (2) ◽  
pp. 327-334 ◽  
Author(s):  
Mihaela NICULAE ◽  
Laura STAN ◽  
Emoke PALL ◽  
Anamaria Ioana PAȘTIU ◽  
Iulia Maria BALACI ◽  
...  

The study was aimed to characterize the chemical composition and the antimicrobial activity of Romanian propolis ethanolic extracts (EEP) against antibiotic-sensitive and antibiotic-resistant E. coli strains isolated from bovine mastitis. The preliminary antimicrobial screening was performed by a disk diffusion method, followed by determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) based on broth microdilution assay; further, the synergistic action of propolis with antimicrobial drugs was assessed by a disk diffusion method on agar containing subinhibitory concentrations of propolis. For the chemical characterisation of EEP, the flavonoids (flavones/flavonols, flavanones/dihydroflavonols) and total phenolics were evaluated by spectrophotometric methods. The phenolic compounds of these extracts were also determined using HPLC. The results indicated for Romanian propolis ethanolic extracts the typical poplar composition profile with flavonoids and phenolic acids as main biological active compounds, with chromatographic analysis data confirmed also spectrophotometrically. In addition, positively correlated with the chemical composition, a strong antimicrobial efficacy was exhibited towards E. coli strains, along with interesting synergistic interaction with antibiotics that can be further investigated to obtain propolis-based formulation with antibacterial properties. Subsequent in vitro and in vivo studies evaluating the safety and efficacy are intended to consider propolis in veterinary therapeutic protocols.


Heliyon ◽  
2020 ◽  
Vol 6 (10) ◽  
pp. e05119
Author(s):  
Florencia Menis Candela ◽  
Walter Fabián Giordano ◽  
Patricia Liliana Quiroga ◽  
Franco Matías Escobar ◽  
Fernando Mañas ◽  
...  

InterConf ◽  
2021 ◽  
pp. 214-218
Author(s):  
Monika Stanciauskaite ◽  
Daiva Majiene ◽  
Liudas Ivanauskas ◽  
Kristina Ramanauskiene

The aim of this study was to prepare liquid aqueous and ethanolic extracts of poplar buds and to investigate their chemical composition.


2018 ◽  
Vol 19 (2) ◽  
Author(s):  
Magdalena Woźniak ◽  
Marta Babicka ◽  
Iwona Rissmann ◽  
Bogdan Kędzia ◽  
Izabela Ratajczak

Introduction. Propolis is one of bee products, which is increasingly widespread in medicine. An important aspect regarding propolis is research on the analysis of its chemical composition. Propolis extracts have been found to include phenolic compounds (flavonoids as well as phenolic acids and their esters), terpenes, amino acids, vitamins and elements. The elements analyzed in the propolis extracts include both macro- and microelements, such as: calcium, magnesium, iron or selenium. Aim. The aim of the study was to determine concentration of selected elements in commercial available ethanolic extracts of propolis. Material and methods. In three ethanolic extracts of propolis were determined concentration of 14 elements (Ca, Mg, K, Na, Zn, Si, Pb, Co, Cr, Cu, Fe, Mn, Cd and Ni) using flame atomic absorption spectrometry. Results. In all propolis extracts the highest concentration among analyzed elements was determined for sodium, and in the lowest level of concentration was analyzed for copper and manganese. The concentrations of analyzed elements in extracts differed slightly and the highest differences were observed for magnesium, potassium and copper. In all examined samples the concentration of heavy metals was under detection limit of used analytical method. Conclusions. The results of the research presented in the paper indicate that the ethanolic extracts of propolis, despite slightly differences in the concentrations of the analyzed elements, can be a rich source of macro- and microelements.


2017 ◽  
Vol 47 (3) ◽  
Author(s):  
Daiane Martini ◽  
Giselle Feliciani Barbosa ◽  
Rosemary Matias ◽  
Wolff Camargo Marques Filho ◽  
Nayara Zielasko Trombini Garcia

ABSTRACT: Apis melífera L. propolis is a resinous and balsamic material whose biological effects are related to its chemical composition. This chemical composition is greatly influenced by seasonality, so propolis from different seasons and regions has a different chemical composition. The increasing need for natural options to control fungi that cause damage to food crops makes propolis an alternative that deserves more research. In this context, the aim of this study was to evaluate the effect of seasonality on the antifungal potential of propolis collected in Campo Grande, Mato Grosso do Sul, Brazil, on the fungus Lasiodiplodia theobromae . Effects of different concentrations of ethanolic extracts of green propolis on the mycelial growth of the pathogen were evaluated. Concentrations of flavonoids and phenolic compounds in the samples were also determined by spectrophotometric methods. Results showed that the propolis extracts have a different chemical composition, potential fungi static effects on the tested fungus, and that there is interference of seasonality on the mycelial growth of the fungus, pointing to the concentration of 1250µg 100mL-1of the samples collected in the summer, in a first moment, as the most efficient.


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