Phytotherapy of ulcerative dermatitis induced by Aeromonas hydrophila infection in goldfish ( Carassius auratus )

2010 ◽  
Vol 58 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Ramasamy Harikrishnan ◽  
Chellam Balasundaram ◽  
Young-Gun Moon ◽  
Man-Chul Kim ◽  
Ju-Sang Kim ◽  
...  

Goldfish ( Carassius auratus ) weighing 13 ± 2 g were administered intramuscularly a sublethal dose (1.8 × 10 3 cfu/ml) of Aeromonas hydrophila to induce ulcerative dermatitis. On day 3 and day 6 after infection the fish were dip-treated (for 5 min/day) with a tri-herbal concoction of Curcuma longa, Ocimum sanctum and Azadirachta indica (1%). The LD 50 value was recorded at a concentration of 2.3 × 10 4 between 30 and 36 days after infection. In the infected untreated group the cumulative mortality was higher, while in the early-treated group (day 3) there was no mortality. In the late-treated group (6th day) the mortality increased to 23.3% on day 36. In the infected group the size of ulcers progressively increased from 43.3% of the body length on day 18 to 86.7% on day 36. In the early-treated fish the size of ulcers was 23.3% of the body length on day 18; by day 36 after treatment the ulcer had completely healed. In contrast, there was only a moderate recovery in the late-treated group. From the results it can be concluded that early tri-herbal treatment ensures successful recovery from ulcerative dermatitis induced by A. hydrophila .

Author(s):  
Vjatscheslav Kuznetsov ◽  
Vladimir Kuznetsov

The article touches upon the problems of breeding goldfish Carassius Auratus (L.), abundance of juveniles, size and age composition of the catches and the growth of this species in small rivers (for example, the Kazanka River and the lower reaches of the Sviyaga River) in terms of stocking the Kuibyshev Reservoir by goldfish. It has been stated that the process of stocking small rivers with goldfish has much in common with that in the reservoir. Spawning in the Kazanka in 2016 proceeded in two stages, and the gonads contained the roe of 2 portions. The first mass spawning took place from 11 to 20 May, and the second spawning – from 11 to 20 June. Macrophytes, driftwood and washed away roots of trees served as substrate for spawning. During the reproduction period of the species in the river the proportion of females made 75.0%, males - 25.0%. In the lower reaches of the Sviyaga in the period of 1998-2001 the proportion of males in catches made 26.1-65.7%. The maximum number of goldfish larvae and fingerlings in the lower reaches of the Sviyaga was observed in 2007-2009 and in 2014. These years had a similar regime of water level, but different temperature conditions in the spring period. Males in the catches had smaller sizes and body weight, compared with females. In the Kazanka in 2013-2016 the average body length of females in catches ranged within 16.7-23.8 cm, and males – within 15.9-20.6 cm. The age composition of goldfish in the river in 2013 consisted of 4-8 summer fish of 2006-2008 generations. Among them prevailed individuals at the age of 6 (53.1%) of the 2006 generation. In 2014-2016 in the catches there were fish aged 6-9 years old, although there were individuals up to 15 years old. The body length of the similar aged individuals of both sexes was not significantly different. Student criterion between both sexes at different ages ranged from 0.06 to 0.84. Growth of individuals of different generations in 2007-2010 also did not differ reliably. Thus, the average body size of 6- year-old fish of these generations ranged from 17.7 to 18.1 cm. The growth of goldfish in different reaches of the Kuibyshev Reservoir was higher than in the Kazanka, but Fulton fatness coefficient in the river was lower. The average values of the fatness coefficient ranged from 3.24 to 3.63 in the river, and in the reservoir reaches they are from 2.93 to 3.07.


1968 ◽  
Vol 25 (9) ◽  
pp. 1797-1801 ◽  
Author(s):  
Jack H. Gakstatter

Immediately after goldfish (Carassius auratus (Linnaeus)) had been exposed for 8 hr in a static system to 0.05 ppm of 14C-aldrin, 14C-dieldrin was detected in various tissues. The percentages that were dieldrin increased with time in all of the tissue studied until at 32 days they were 93.9% or more except for visceral fat. Except in visceral fat, 50 and 100% of the residues were dieldrin within 2.5–5.4 and 31.5–92.4 days respectively. For visceral fat the corresponding times were 46.9 and 14,733 days.


2018 ◽  
Vol 65 (4) ◽  
Author(s):  
Arathi Dharmaratnam ◽  
T.Raja Swaminathan ◽  
Raj Kumar ◽  
V.S. Basheer

Aeromonas hydrophila was identified the causative agent of a disease outbreak in goldfish, Carassius auratus from four ornamental fish farms in Kerala, India. Seven bacterial isolates viz., Plesiomonas shigelloides (NPPS-1), Aeromonas hydrophila (NPAH-1, 2, 3 and 4), Citrobacter freundii (NPCF-1) and Acinetobacter spp. (NPA-1) were isolated from the affected fish. Further, all four A. hydrophila (NPAH-1, 2, 3 and 4) isolates were identified by amplification of gyrB and rpoD genes. The RAPD profile using 3 primers confirmed that all four A. hydrophila were genetically similar. No cytopathic effect was observed on goldfish fin (GFF) cell line after inoculation of the tissue homogenate from the affected fish and affected tissues were found negative for koi herpesvirus (KHV), cyprinid herpesvirus-2 (CyHV-2) and spring viraemia of carp virus (SVCV). Experimental challenge resulted in mortality of fish injected with A. hydrophila only. A. hydrophila was observed to be cytotoxic on GFF cell line and exhibited haemolytic activity on 5% sheep blood agar. A. hydrophila possessed multiple virulence genes viz., enterotoxins, haemolytic toxins and outer membrane protein as determined by PCR. A. hydrophila was sensitive to Cefixime, Chloramphenicol, Nitrofurantoin, Kanamycin, Ciprofloxacin, Furazolidone and Cefixime/Clavulanic acid. After treatment with the suggested antibiotics, the fish were recovered from the disease.


1952 ◽  
Vol 3 (2) ◽  
pp. 126 ◽  
Author(s):  
RJ Affleck

The colours of nacreous (mottled) fish are usually mottled but specimens do occur in which only one kind of the black, orange, or yellow chromatophores is present, while some lack pigments over most of the body. The colours appear metallic, pearl, or matt depending on the presence or absence of reflecting tissue in two definite layers. The hues seen in these fish are black, smoke, blue, orange, yellow, blood-red, and silver, which occur singly or in combinations. These hues are produced by black, orange, and yellow pigments in chromatophores, silver reflectillg tissue, and haemoglobin in the blood. The presence of a yellow pigment — a carotenoid — is reported for the first time. Although the colour red has been reported no red pigment has ever been described. An intense orange pigment will produce a red effect on a pale blood-red background. The nacreous (mottled) group of the goldfish is intermediate between the metallic (scaled) and matt (transparent) groups with regard to the amount of reflecting tissue. The colours of these fish may not be used as criteria of the groups.


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