New HPTLC method, with systematic mobile-phase optimization, for determination of six apolar heterocyclic aromatic amines

2004 ◽  
Vol 17 (100) ◽  
pp. 431-434 ◽  
Author(s):  
Gertrud Morlock
Keyword(s):  
Aromatic Amines ◽  
Mobile Phase ◽  
Heterocyclic Aromatic Amines ◽  
Mobile Phase Optimization ◽  
Phase Optimization ◽  
Hptlc Method

Suitability of ionic liquids as mobile-phase additives in HPLC with fluorescence and UV detection for the determination of heterocyclic aromatic amines

2010 ◽  
Vol 33 (2) ◽  
pp. 182-190 ◽  
Author(s):  
Aurora Martín-Calero ◽  
Gracian Tejral ◽  
Juan H. Ayala ◽  
Venerando González ◽  
Ana M. Afonso
Keyword(s):  
Ionic Liquids ◽  
Aromatic Amines ◽  
Mobile Phase ◽  
Uv Detection ◽  
Heterocyclic Aromatic Amines ◽  
Mobile Phase Additives

RP-HPLC AND HPTLC STABILITY INDICATING ASSAY METHODS FOR IVERMECTIN IN BULK AND TABLET DOSAGE FORM

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (03) ◽  
pp. 32-42
Author(s):  
G. P Wani ◽  
◽  
S. B Jadhav
Keyword(s):  
Mobile Phase ◽  
Stress Testing ◽  
Hplc Method ◽  
Rp Hplc ◽  
Assay Methods ◽  
Photo Stability ◽  
Tablet Dosage ◽  
Hptlc Method ◽  
Alkaline Oxidation

Simple, rapid, precise, accurate RP-HPLC and HPTLC methods have been developed and validated for ivermectin in bulk and its marketed formulation. RP-HPLC method for drug was achieved on Grace C18 (250 mm X 4.6 ID, Particle size; 5 μ) column using mobile phase acetonitrile: 10 mM phosphate buffer (95:05 v/v) pH adjusted to 3 with o-phosphoric acid. Detection of drug was done at 245 nm. The retention time was found to be 5.83 min. HPTLC method for ivermectin was accomplished on a precoated silica gel aluminium plate 60F-254 (CAMAG Linomat 5), using toluene: methanol: glacial acetic acid (8:2:0.1 v/v/v) as a mobile phase. The densitometric scanning was performed at 245 nm which showed Rf 0.46 for ivermectin. The stress testing of the drug individually was carried out under acidic, alkaline, oxidation, photo-stability and thermal degradation conditions. The proposed methods were successfully applied for the determination of drug in bulk and its marketed formulation.

Mobile Phase Optimization in RPLC by an Iterative Regression Design

2021 ◽  
pp. 63-104
Author(s):  
Leo de Galan ◽  
Hugo A. H. Billiet
Keyword(s):  
Mobile Phase ◽  
Mobile Phase Optimization ◽  
Regression Design ◽  
Phase Optimization

Determination of heterocyclic aromatic amines by capillary electrophoresis coupled to mass spectrometry using in-line preconcentration

2003 ◽  
Vol 24 (17) ◽  
pp. 3075-3082 ◽  
Author(s):  
Sònia Sentellas ◽  
Encarnación Moyano ◽  
Lluís Puignou ◽  
M. Teresa Galceran
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Aromatic Amines ◽  
Heterocyclic Aromatic Amines

Determination of heterocyclic aromatic amines (HAs) content in samples of household-prepared meat dishes

2004 ◽  
Vol 802 (1) ◽  
pp. 95-106 ◽  
Author(s):  
L. Warzecha ◽  
B. Janoszka ◽  
U. Błaszczyk ◽  
M. Stróżyk ◽  
D. Bodzek ◽  
...  
Keyword(s):  
Aromatic Amines ◽  
Heterocyclic Aromatic Amines

scholarly journals Determination of Delafloxacin in Pharmaceutical Formulations Using a Green RP-HPTLC and NP-HPTLC Methods: A Comparative Study

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 359 ◽  
Author(s):  
Prawez Alam ◽  
Essam Ezzeldin ◽  
Muzaffar Iqbal ◽  
Gamal A.E. Mostafa ◽  
Md. Khalid Anwer ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Silica Gel ◽  
Solid Lipid Nanoparticles ◽  
Ternary Mixture ◽  
Mobile Phase ◽  
Pharmaceutical Formulations ◽  
Lipid Nanoparticles ◽  
Ammonia Solution ◽  
Hptlc Method

In this work; delafloxacin (DLFX) was determined using a validated green RP-HPTLC and NP-HPTLC methods in commercial tablets and in-house developed solid lipid nanoparticles (SLNs). RP-HPTLC determination of DLFX was performed using “RP-18 silica gel 60 F254S HPTLC plates”. However; NP-HPTLC estimation of DLFX was performed using “silica gel 60 F254S HPTLC plates”. For a green RP-HPTLC method; the ternary combination of ethanol:water:ammonia solution (5:4:2 v/v/v) was used as green mobile phase. However; for NP-HPTLC method; the ternary mixture of ethyl acetate: methanol: ammonia solution (5:4:2 v/v/v) was used as normal mobile phase. The analysis of DLFX was conducted in absorbance/reflectance mode of densitometry at λmax = 295 nm for both methods. RP-HPTLC method was found more accurate, precise, robust and sensitive for the analysis of DLFX compared with the NP-HPTLC method. The % assay of DLFX in commercial tablets and in-house developed SLNs was determined as 98.2 and 101.0%, respectively, using the green RP-HPTLC technique, however; the % assay of DLFX in commercial tablets and in-house developed SLNs was found to be 94.4 and 95.0%, respectively, using the NP-HPTLC method. Overall, the green RP-HPTLC method was found superior over the NP-HPTLC. Therefore, the proposed green RP-HPTLC method can be successfully applied for analysis of DLFX in commercial tablets, SLNs and other formulations containing DLFX.

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