scholarly journals SPECIFIC GYRB SEQUENCE OF MYCOBACTERIUM TUBERCULOSIS CLINICAL ISOLATED FROM SPUTUM OF PULMONARY TUBERCULOSIS PATIENTS IN INDONESIA

2014 ◽  
Vol 3 (3) ◽  
pp. 143
Author(s):  
N. M. Mertaniasih ◽  
N. Wiqoyah ◽  
D. Kusumaningrum ◽  
S. Soedarsono ◽  
A. D. S. Perwitasari ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Tuberculosis Patients ◽  
Gyrb Sequence

Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Accurate Method ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Diagnostic Potential ◽  
Total Death ◽  
Smear Negative ◽  
Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Syoof Khowman Alramahy ◽  
Akram Hadi Hamza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Statistical Difference ◽  
Positive Culture ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
The Mean ◽  
Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

scholarly journals Gambaran leukosit urin pada pasien tuberkulosis paru dewasa di RSUP Prof. Dr. R. D. Kandou Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Pingkan C. Pasuhuk ◽  
Arthur E. Mongan ◽  
Mayer Wowor
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Dipstick Test ◽  
Chemical Test ◽  
Tuberculosis Patients ◽  
Negative Results ◽  
Normal Urine ◽  
Observational Descriptive Study ◽  
Positive Results ◽  
Urine Specimens

Abstract: Tuberculosis (TB) is an infectious diseases caused by Mycobacterium Tuberculosis. Consuming the anti-tuberculosis medicine such as streptomycin and rifampicin will cause nephrotoxic effect. In kidney disfunction especially the glomeruli, the number of leukocytes in the urine increase. The most common urinalysis tests are chemical test and microscopic test, especially the leukocyte urine test. These tests can be used to detect the kidney disfunction and the urinary tract infection. In normal urine, the result of the dipstick test is negative, and the result of microscopic test is 0-5/HPF. This study was aimed to obtain the description of leukocyte urinalysis in pulmonary tuberculosis patients at Prof. Dr. R. D. Kandou Hospital Manado. This was an observational descriptive study conducted in October-November 2016 at Prof. Dr. R. D. Kandou Hospital Manado. Samples were random urine specimens that met the predefined criteria. The results showed that based on the urinalysis, of 30 patients with pulmonary tuberculosis, 27 patients had negative results and 3 patients had positive results. Conclusion: There was no relationship between urine leucocyte and pulmonary tuberculosis in adult patients.Keywords: pulmonary tuberculosis, urinalysis, urine leukocyte Abstrak: Tuberkulosis (TB) merupakan penyakit infeksi yang disebabkan oleh Mycobacterium tuberculosis. Obat anti-tuberkulosis seperti streptomisn dan rifampisin memiliki efek nefrotoksik. Kerusakan ginjal terutama glomerulus dapat menimbulkan peningkatan leukosit dalam urin. Metode urinalisis yang sering digunakan yaitu uji kimia/ carik-celup dan mikroskopik. Pemeriksaan leukosit urin dapat digunakan untuk mengetahui adanya gangguan pada ginjal dan infeksi saluran kemih. Pada urin normal hasil pemeriksaan dipstick negatif dan hasil pemeriksaan mikroskopik urin 0-5 leukosit/LPB. Penelitian ini bertujuan untuk mendapatkan gambaran leukosit urin pasien tuberkulosis paru dewasa di RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian ialah deskriptif observasional dan dilakukan pada bulan Oktober-November 2016 di RSUP Prof. Dr. R. D. Kandou Manado. Sampel penelitian ialah sampel urin sewaktu dari semua pasien tuberkulosis paru yang memenuhi kriteria yang telah ditentukan. Hasil urinalisis dari 30 pasien terdiagnosis penyakit tuberkulosis paru mendapatkan 27 pasien dengan hasil negatif dan 3 pasien dengan hasil positif. Simpulan: Tidak terdapat hubungan antara leukosit urin dengan tuberkulosis paru dewasa. Kata kunci: TB paru, urinalisis, leukosit urin

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