Determination some Immunological Aspects in Pulmonary Tuberculosis Patients in Qadisiyah Province

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Syoof Khowman Alramahy ◽  
Akram Hadi Hamza
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Statistical Difference ◽  
Positive Culture ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
The Mean ◽  
Igg Level

This study was carried out to study of some immunological aspects among the pulmonary Tuberculosis patients infected with causative agent, Mycobacterium tuberculosis. A Total of 200 sputum samples were collected from patients attending the consultant Clinic for Chest and Respiratory disease center, Diwaniya. Control group (No=15) also included. According to acid fast stain of sputum, the patients were classified as positive (No=91,45.5%) and negative (No=109,54.5, Lowenstein Jensen medium used for the cultivation of samples, on which 70% of sputum samples where positive culture for this microorganism. The grown microorganism were identified as M. tuberculosis, based on positive A.F.B, Niacin producers ,negative for catlase at 68c. The mean IgG level was l184.053±76.684 mg/100 ml in tuberculosis group compared with 1016.533 ± 44.882 mg/100ml in control group, rendering the statistical difference significant. For IgA and IgM levels, they were at mean of 315.880±38.552 mg/100 ml and 119.527±8.464 mg/100 ml in control group compared with 396.358±38.776 mg/100 ml and 134.207±11.696 mg/100 ml in patients group respectively with significant difference

scholarly journals Metode mikrokoloni (slide culture) sebagai metode diagnostic alternative yang lebih cepat untuk diagnosis tuberculosis paru

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Eko Budi Koendhori ◽  
Setio Harsono
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Wide Distribution ◽  
Tuberculosis Patients ◽  
Slide Culture ◽  
Significant Difference ◽  
Lowenstein Jensen ◽  
Time Required ◽  
Microscope Slides ◽  
Method Analysis

Despite wide distribution of pulmonary tuberculosis in Indonesia, its diagnosis is still an important issue to be dealt with. Fourty seven sputums from pulmonary tuberculosis patients in Surabaya were examined to detect Mycobacterium tuberculosis using three methods, i.e. the acidfast stain Ziehl Neelsen, microcolony (slide culture) and Lowenstein Jensen. Sputums were collected spontaneously from the patients. All of them were decontaminated and centrifuged. After the supernatant fluids were carefully decanted, the sediments were resuspended in 1 ml of 10 mMphosphate buffer (pH 7.4) and the suspensions were then inoculated on to two 76 x 13 mm glass microscope slides. One of them was stained by Ziehl Neelsen method and the other was inoculated into microcolony media for seven days and the waste suspension was inoculated into LowensteinJensen media. The results of the microcolony method analysis were compared with the Ziehl Neelsen staining. Employing McNemar test, a significant difference was observed between the microcolony method and the Ziehl Neelsen staining (?² = 5,88). The sensitivity and spesificity ofmicrocolony were 100% and 89% while the Ziehl Neelsen were 60% and 84% respectively. In conclusions microcolony method was better compared with the Ziehl Neelsen staining in the detection of Mycobacterium tuberculosis. Microcolony method was able to reduce time required todetect Mycobacterium tuberculosis in patient suspected with pulmonary tuberculosis.

scholarly journals Poor Performance of a Novel Serological Test for Diagnosis of Pulmonary Tuberculosis in Bangui, Central African Republic

2006 ◽  
Vol 13 (6) ◽  
pp. 702-703 ◽  
Author(s):  
Eric Kassa-Kelembho ◽  
Edith Kassa ◽  
Germain Zandanga ◽  
Yves-Brillant Service ◽  
Albert Ignaleamoko ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Microscopic Examination ◽  
Central African Republic ◽  
Serological Test ◽  
Positive Culture ◽  
Poor Performance ◽  
Sputum Smear ◽  
Tuberculosis Patients

ABSTRACT We assessed the performance of a serological test for tuberculosis (SDHO Laboratories Inc., Canada) in our setting. Among 68 of 99 suspected pulmonary tuberculosis patients who were scored as having tuberculosis on the basis of Mycobacterium tuberculosis-positive culture, the sensitivity of the serological test was lower than that of sputum smear microscopic examination (20.6% versus 80.9%, respectively; P < 0.000001).

scholarly journals Diagnostic value of serum IgG by Eliza to detecting Mycobacterium tuberculosis, Original Article

2021 ◽  
Vol 9 (1) ◽  
pp. 29-29
Author(s):  
Mehdi Haghdoost ◽  
Parisa Alizadeh Nazmi ◽  
Hamid Owaysee Osquee
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Immunoglobulin G ◽  
Diagnostic Value ◽  
World Health ◽  
Control Group ◽  
Reference Laboratory ◽  
Predictive Values ◽  
Serum Igg ◽  
Significant Difference

Introduction: In developing countries, which is an endemic region in terms of tuberculosis, there is an urgent need for fast, accurate, and inexpensive serological testing. The aim of this study was to determine the diagnostic value of patient serum IgG antibodies by ELISA in the diagnosis of Mycobacterium tuberculosis. Method: This case-control study was performed on patients with pulmonary tuberculosis in 2017-2020. After selecting the case (n = 30) and control (n = 30) subjects according to inclusion criteria, their blood samples were obtained and analyzed in the reference laboratory by standard kits for immunoglobulin G against 16, 36, and 40 kDa antigens of mycobacterium tuberculosis. Results: The mean age of the subjects was 47.07 (15.57%). The majority of participants were 46 (51.1%) women. There was no significant difference between the two groups regarding sex and age. serological examination of patients with pulmonary tuberculosis showed 25 positive results and only 4 of the control group had a positive result. Sensitivity, specificity, positive and negative predictive values of serology test were 83.3%, 86.67%, 86.20% and 87.88% respectively. Conclusion: Despite the acceptable sensitivity of the serologic immunoglobulin G test, according to the statement of World health organization (WHO), it did not possess an acceptable specificity. It is recommended that a a wider range of different antigens to be studied also it is essential to evaluate the diagnostic value of the other immunoglobulins inpatient in different stages of the disease.

scholarly journals DIFFERENCES OF INTERLEUKIN-18 AND INTERLEUKIN-10 LEVELS IN RIFAMPICIN RESISTANT AND RIFAMPICIN SENSITIVE PULMONARY TUBERCULOSIS PATIENTS IN DR. SOETOMO HOSPITAL SURABAYA

2020 ◽  
Vol 8 (2) ◽  
pp. 116
Author(s):  
Audrey Gracelia Riwu ◽  
Jusak Nugaraha ◽  
Yoes Prijatna Dachlan
Keyword(s):  
Pulmonary Tuberculosis ◽  
Inflammatory Cytokine ◽  
Interleukin 10 ◽  
Interleukin 18 ◽  
Research Subjects ◽  
Short Term Treatment ◽  
Tuberculosis Patients ◽  
Pulmonary Tb ◽  
Significant Difference ◽  
The Mean

Rifampicin is an anti-tuberculosis drug which has an efficient antimicrobial effect and the basis of a short-term treatment regimen for tuberculosis (TB) patients. Rifampicin plays an important role against the growth and slow metabolism of Bacilli M. tuberculosis. Resistance to rifampicin causes the duration of tuberculosis treatment to be longer. Interleukin-18 (IL-18) is a pro-inflammatory cytokine which plays a role in controlling the growth of M. tuberculosis through its ability to induce IFN-γ, while Interleukin-10 (IL-10) is an anti-inflammatory cytokine which plays a role in limiting tissue damage due to the inflammatory process and maintain tissue homeostasis. IL-18 and IL-10 has an important role in explaining the different degrees of inflammation in rifampicin resistant (RR) and rifampicin sensitive (RS) pulmonary tuberculosis patients. The purpose of this study is to determine different levels of IL-18 and IL-10 in new TB patients with RR and RS. This study was a retrospective cohort study with a cross-sectional design carried out from August-November 2018 in the TB-DOTS/MDR clinic at Dr. Soetomo Hospital, Surabaya. 50 research subjects were examined and grouped into two groups, namely pulmonary TB with RR (n = 25) and pulmonary TB with RS (n = 25) based on GeneXpert examination and anti-tuberculosis drug therapy ≤ 1 month. IL-18 and IL-10 were measured using the ELISA Method. Differences in IL-18 and IL-10 levels between groups were analyzed using the Mann-Whitney test. The mean level of IL-18 (pg/ml) in RR and RS pulmonary TB patients were 1273.53±749.86 and 787.96 ±589.28 respectively. The mean level of IL-10 (pg/ml) in RR and RS pulmonary TB patients were 125.25±118.32 and 128.81±135.77 repectively. The mean level of IL-18 in RR and RS pulmonary TB patients were found to have a significant difference, while the mean level of IL-10 did not have a significant difference. Keywords: Interleukin-18, Interleukin-10, Tuberculosis, Rifampicin Resistant, Rifampicin Sensitive

scholarly journals Evaluating the Humoral Immunity and Interleukin 18 Receptor 1 in some Patients with Molluscum Contagiosum Infection

2016 ◽  
Vol 13 (1) ◽  
pp. 66-73
Author(s):  
Baghdad Science Journal
Keyword(s):  
Molluscum Contagiosum ◽  
The Body ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Interleukin 18 ◽  
Molluscum Contagiosum Virus ◽  
Significant Difference ◽  
The Mean ◽  
Diffusion Assay ◽  
Igg Level

The molluscum contagiosum virus (MCV) is a dermatotropic poxvirus. The causative agent of molluscum contagiosum (MC) is nonlethal, common and worldwide. Additionally, little inflammation is associated with MC papules. The present study aims to evaluate the immune status of MC patients by measuring the level of immunoglobulins IgG and IgM by using the radial immune diffusion assay (RIA) and the level of interleukin 18 receptor 1 (IL-18R1) by the Enzyme-linked immunosorbent assay (ELISA).The study is conducted during November 2013 to April, 2014 in outpatient clinic of Baquba Teaching Hospital. There are 75 patients, diagnosed with clinical lesions of MCV on different areas of the body, whose age is ranged between 2-50 years including 40(53.3%) males and 35(46.7%) females. The study includes 15 healthy persons age between 2-50 years. The level of IL 18R1 were significantly elevated in patients (677.15±874.22) compared with control (178.46±31.79 ng/ml). There is also a significant elevation in the mean level of serum IgM, where it is 1946.6±825.6 mg/dl while in control group is 140.1±68.7mg/dl. By contrast in patients with lower levels of IgG than the control, the mean serum IgG level in patient is 221.9±96.7 mg/dl while in the control is 1229.9±299.7 mg/dl. Finally, there is no significant difference between MC patients from rural area and urban area.

scholarly journals Detection of Serum Levels of IL-17 and CCL-5 in a Sample of Iraqi Pulmonary Tuberculosis Patients

2021 ◽  
pp. 2887-2893
Author(s):  
Entssar S. Hafid ◽  
May K. Ismael
Keyword(s):  
Pulmonary Tuberculosis ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Interleukin 17 ◽  
Cell Trafficking ◽  
Tuberculosis Patients ◽  
Cytokines And Chemokines ◽  
Immune Tissues ◽  
The Mean

      Cytokines and chemokines are small-secreted proteins involved in many aspects of cell development, differentiation, and activation functions. A prominent characteristic of these molecules is their effect on the immune system in relation to the development of cell trafficking and immune tissues and organs. Furthermore, they play an important role in initiating and coordinating the organized and sequential recruitment and activation of cells into Mycobacterium tuberculosis-infected lungs. We aimed to evaluate the levels of interleukin -17 (IL-17) and the chemotactic chemokine (C-C motif) ligand 5 (CCL5) in the sera of pulmonary tuberculosis (PTB) patients. About 90 subjects were included, involving 50 patients with pulmonary TB and 40 apparently healthy individuals who were selected as a control group. Sera were obtained for measuring IL-17 and CCL-5 levels by enzyme linked immunosorbent assay (ELISA). The results revealed that serum levels of IL-17 showed no significant differences between each patient's group and control. In contrast, the serum level of CCL-5 was significantly increased in pulmonary tuberculosis patients compared to control (P ≤0.01). The mean ±SE values of IL-17 level in PTB patients and controls were 43.06 ±3.64 and 41.009 ± 0.009 pg/ml, respectively. While, the mean ±SE values of CCL-5 level in PTB patients and controls were 455.40 ±25.35 and 80.86 ± 5.96 ng/L, respectively. The results of the current study suggest that high levels of CCL-5 in the sera of PTB patients may indicate an important role in the immunopathogenesis of the disease. Therefore, this chemokine could be considered as a useful biomarker for the severity of PTB infections.

Tocovid, a tocotrienol-rich vitamin E in preventing atrial fibrillation in post-coronary artery bypass grafting (CABG) surgery: A preliminary result

2021 ◽  
Author(s):  
Ahmad Farouk Musa ◽  
Jeswant Dillon ◽  
Mohamed Ezani Md T ◽  
Alwi Mohamed Yunus ◽  
Abdul Rais Sanusi ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Vitamin E ◽  
Hospital Stay ◽  
Incidence Rate ◽  
Statistical Difference ◽  
Control Group ◽  
Inflammatory Agent ◽  
Significant Difference ◽  
The Mean ◽  
Anti Inflammatory

Abstract Introduction: Post-operative atrial fibrillation (POAF) is associated with poor outcome, increased resource utilisation, morbidity and mortality. Its pathogenesis is initiated by systemic inflammation and oxidative stress. It is hypothesised that a potent antioxidant and anti-inflammatory agent such as tocotrienol, an isomer of Vitamin E, could prevent POAF.Aims: The aim of this study is to determine whether a potent antioxidative and anti-inflammatory agent, Tocovid, a tocotrienol-rich capsule, could reduce the incidence of POAF and affect the mortality and morbidity as well as the duration of ICU, HDU and hospital stay. Methods: This study was planned as a prospective, randomised, controlled trial with parallel groups. The control group received placebo containing palm superolein while the treatment group received Tocovid capsules. We investigated the incidence of POAF, the length of hospital stay (LoHS) after surgery and the health-related quality of life (HRQoL). Results: The recruitment started in January 2019 but the preliminary results are unblinded since the study is still ongoing. 202 patients have been recruited out of a target sample size of 250 as of January 2021. About 75% have completed the study and 6.4% were either lost during follow-up or withdrawn; 4% of them died. The mean age group was 61.44 ± 7.30 with no statistical difference between them, with males having a preponderance for AF. The incidence rate of POAF was 24.36% and the mean time for developing POAF was 55.38 ± 29.9 hours post-CABG. Obesity is not a predictive factor. No statistically significant difference was observed when comparing left atrial size, NYHA group, ejection fraction and the premorbid history. The mean cross-clamp time was 71 ± 34 minutes and the mean bypass time was 95 ± 46 minutes, with no statistical difference. There was a three-fold increase in death among patients with POAF (p=0.008) and an increase in the duration of ICU stay (p=0.01), the total duration of hospital stay (p=0.04) and reintubation (p=0.045).Conclusion: A relatively lower incidence rate of POAF was noted though the study is still ongoing. It remains to be seen if our prophylactic intervention using Tocovid would reduce the incidence of AF.

Elevated Concentrations of Interleukins and Leukotriene in Response to Mycobacterium Tuberculosis Infection

1997 ◽  
Vol 34 (2) ◽  
pp. 160-164 ◽  
Author(s):  
O El-Ahmady ◽  
M Mansour ◽  
H Zoeir ◽  
O Mansour
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Lipid Extraction ◽  
Mean Value ◽  
Tuberculosis Infection ◽  
Control Group ◽  
Healthy Controls ◽  
Mycobacterium Tuberculosis Infection ◽  
Tuberculosis Patients ◽  
Gm Csf

There is significant research in the role of interleukins in lung disease, as the cytokines are important mediators in the host response to mycobacterium tuberculosis infection. Plasma from patients with pulmonary tuberculosis (TB) and healthy controls were investigated for their content of granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-6 (IL-6) and leukotriene B4 (LTB4). LTB4 and IL-6 were measured by enzyme immunoassay after lipid extraction in the case of LTB4 while GM-CSF was measured by enzyme amplified sensitive immunoassay. Significantly elevated concentrations of IL-6 were found in far-advanced lesions of pulmonary tuberculosis patients, P < 0·05. However, nonsignificant increases of IL-6 were obtained in moderate lesions and minimal lesions compared to normal healthy subjects. Marked elevations of LTB4 were found in TB patients, the highest values being shown in patients with far-advanced lesions followed by moderately advanced and minimal lesions in relation to the mean value for normal healthy controls, P < 0·001 for all groups. 93% of the tuberculosis patients showed a higher level of LTB4 above the upper limit of the control group. In contrast there was no significant increase of GM-CSF in any of the TB subgroups. These results suggest that LTB4 and the interleukins may play a role in the pathogenesis of mycobacterium tuberculosis infection.

scholarly journals CORRELATION BETWEEN SERUM CALCIUM LEVELS AND SMEAR GRADING AMONG THE PULMONARY TUBERCULOSIS PATIENTS

2019 ◽  
Vol 3 (3) ◽  
Author(s):  
Pramod Sood ◽  
Sanjay Bhat
Keyword(s):  
Pulmonary Tuberculosis ◽  
Causes Of Death ◽  
Statistical Significance ◽  
Control Group ◽  
Healthy Individuals ◽  
Tuberculosis Patients ◽  
Cause Of Deaths ◽  
The Mean ◽  
The Difference ◽  
Group Serum

Background: Tuberculosis (TB) is contagious and airborne. TB was one of the top 10 causes of death worldwide in 2017.  It is also the leading killer of people with HIV and a major cause of deaths related to antimicrobial resistance. Methods: Microscopically confirmed TB patients were included in the cases group and healthy individuals in the control group. Serum Ca was estimated by kit method, by auto analyzer. ANOVA and t- test was used to find the statistical significance. Results: The mean serum Ca was 7.32±1.22 mg/dl in TB and 9.41±2.13 mg/dl in controls. The mean serum Ca levels were 7.22±1.20 mg/dl, 7.14±1.14 mg/dl, 7.12±1.10 mg/dl and 7.06±1.02 mg/dl respectively for scanty, 1+. 2+ and 3+; statistically the difference were not significant (P > 0.05) between the grades. Conclusion: Hypocalcemia observed in pulmonary tuberculosis patients. No correlation was found between the mean serum Ca levels and grading. Keywords: Calcium, TB, AFB.

scholarly journals The Impact of Heart Disease on the Characteristics of Pulmonary Tuberculosis: The Extent of Pulmonary Involvement, Evolution under Treatment & Treatment Outcome

2020 ◽  
pp. 1-4
Author(s):  
Anass Benali ◽  
Anass Benali ◽  
Ismail Abderrahmani Rhorfi ◽  
Hicham Souhi ◽  
Hanane El Ouazzani ◽  
...  
Keyword(s):  
Heart Disease ◽  
Pulmonary Tuberculosis ◽  
Pulmonary Involvement ◽  
Control Group ◽  
Tuberculosis Patients ◽  
Risk Of Death ◽  
Significant Difference ◽  
History Of ◽  
The Impact

Methods: We report a retrospective study analysing the characteristics of pulmonary tuberculosis in patients with pulmonary tuberculosis with heart disease and in tuberculosis patients with no obvious history, treated in the pulmonology department between 2017 and June 2020. Results: Tuberculosis patients with heart disease present more extensive forms than patients without a history (p <0.001), with more occurrence of treatment failure and sequelae (p = 0.01), and more risk of death compared to the control group without heart disease but without significant difference compared to the literature. While the two groups did not differ in terms of cure and negativation of bacteriological tests after two months of treatment (p> 0.05). Conclusion: Heart disease has a significant impact on the history of pulmonary tuberculosis therefore they affect the quality of life of patients.

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