Analysis of Successive Focused Ion Beam Slices by Scanning Electron Imaging and 3D Reconstruction

2005 ◽  
Vol 908 ◽  
Author(s):  
Terence Yeoh ◽  
Neil Ives ◽  
Nathan Presser ◽  
Gary Stupian ◽  
Martin Leung ◽  
...  
Keyword(s):  
Focused Ion Beam ◽  
Ion Beam ◽  
Three Dimensional ◽  
Complex Mixture ◽  
Dimensional Reconstruction ◽  
Microscope Imaging ◽  
End Point Detection ◽  
Electron Imaging ◽  
Point Detection ◽  
Scanning Electron

AbstractAn antifuse structure was analyzed using scanning electron microscope imaging and focused ion beam image slicing to generate a form of three-dimensional microscopy. This method reveals nanometer scale features that could not be easily imaged using a single focused ion beam cross-section. A novel end-point detection technique has been developed to control the thickness of the slice to about 2 nm. Voxel imaging and interpretive three-dimensional reconstruction was used to resolve volumes as small as 2 cubic nm3. It was determined that the fusing region for an antifuse is a complex mixture of material phases with an elliptical volume approximately 75 nm in diameter.

Three Dimensional Reconstruction of Solid Oxide Fuel Cell Electrodes Using Focused Ion Beam - Scanning Electron Microscopy

2019 ◽  
Vol 7 (1) ◽  
pp. 1879-1887 ◽  
Author(s):  
James Wilson ◽  
Worawarit Kobsiriphat ◽  
Roberto Mendoza ◽  
Hsun-Yi Chen ◽  
Tom Hines ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Three Dimensional ◽  
Solid Oxide ◽  
Oxide Fuel ◽  
Fuel Cell Electrodes ◽  
Dimensional Reconstruction ◽  
Scanning Electron

scholarly journals Nephrocytes are part of the spectrum of filtration epithelial diversity

2020 ◽  
Vol 382 (3) ◽  
pp. 609-625
Author(s):  
Takayuki Miyaki ◽  
Yuto Kawasaki ◽  
Akira Matsumoto ◽  
Soichiro Kakuta ◽  
Tatsuo Sakai ◽  
...  
Keyword(s):  
Body Fluid ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Three Dimensional ◽  
Model System ◽  
The Body ◽  
Toxic Substances ◽  
Cellular Architecture ◽  
Animal Phylogeny ◽  
Scanning Electron

AbstractThe excretory system produces urine by ultrafiltration via a filtration epithelium. Podocytes are widely found as filtration epithelial cells in eucoelomates. In some animal taxa, including insects and crustaceans, nephrocytes serve to separate toxic substances from the body fluid, in addition to podocytes. Drosophila nephrocytes have been recently utilized as a model system to study podocyte function and disease. However, functionality and cellular architecture are strikingly different between Drosophila nephrocytes and eucoelomate podocytes, and the phylogenetic relationship between these cells remains enigmatic. In this study, using focused-ion beam-scanning electron microscopy (FIB-SEM) tomography, we revealed three-dimensional architecture of decapod nephrocytes with unprecedented accuracy—they filled an enormous gap, which can be called “missing link,” in the evolutionary diversity of podocytes and nephrocytes. Thus, we concluded that nephrocytes are part of the spectrum of filtration epithelial diversity in animal phylogeny.

scholarly journals FIB-SEM Three-Dimensional Tomography for Characterization of Carbon-Based Materials

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Nan Nan ◽  
Jingxin Wang
Keyword(s):  
Focused Ion Beam ◽  
Ion Beam ◽  
Three Dimensional ◽  
3D Tomography ◽  
Tomography System ◽  
Internal Structures ◽  
Potential Applications ◽  
Scanning Electron ◽  
Carbon Based

A review on the recent advances of the three-dimensional (3D) characterization of carbon-based materials was conducted by focused ion beam-scanning electron microscope (FIB-SEM) tomography. Current studies and further potential applications of the FIB-SEM 3D tomography technique for carbon-based materials were discussed. The goal of this paper is to highlight the advances of FIB-SEM 3D reconstruction to reveal the high and accurate resolution of internal structures of carbon-based materials and provide suggestions for the adoption and improvement of the FIB-SEM tomography system for a broad carbon-based research to achieve the best examination performances and enhance the development of innovative carbon-based materials.

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